1.Experimental Study of Effect of Acupuncture on Behaviors of Mice Model of Rapid Senilized Dementia
Jingxian HAN ; Ping LI ; Qingzhong LIU
Journal of Traditional Chinese Medicine 1993;0(04):-
By adopting scoring method on the degree of senilization and methods of high cross frame labyrinthine experiment and shuttle box on the basis of systematic observation on behavior characteristics of rapid senilized dementia in SAM - P/8 model, the effect of acupuncture method for wakening the consciousness and opening of orifices was observed. Results showed SAM -P/8 revealed apparent senilized characteristics of brain, being in a low intelligent condition, which is a rather ideal model for the study of natural damentia in the elderly. The acupuncture methods revealed an action of delaying senility process, improving the condition of frightening and increasing the ability of learning and memory.
2.Clinical analysis of nasal sinus mucocele with eye symptoms as main manifestation: 3 cases report.
GU QINGJIA ; LI JINGXIAN ; FAN JIANGANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(7):666-667
Endoscopic sinus surgery is effective to nasal sinus mucocele with eye symptoms as main manifestation. It is very importment to raise the awareness of the disease and to prompt imaging examination. Three cases were reviewed. One mucocele was found in the frontal sinus ethmoid sinus,1 in the fronto-ethmoid sinus and 1 in the spheno-ethmoid sinus. All cases were preoperatively diagnosed by CT, MRI or intranasal endoscopy. Nasal sinus mucocele with eye symptoms as main manifestation should be early diagnosed. Endoscopic sinus surgery is a safe and effective method for the treatment of nasal sinus mucocele,and could be the primary choice for it. All cases were treated by nasal endoscopic sinus surgery. The majority of symptoms, such as exophthalmos, epiphora and diplopia, disappeared in all patients. However, vision recovery was observed only in some patients.
Diplopia
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complications
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Endoscopy
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Ethmoid Sinus
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pathology
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Exophthalmos
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complications
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Frontal Sinus
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pathology
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Humans
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Lacrimal Apparatus Diseases
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complications
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Magnetic Resonance Imaging
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Mucocele
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complications
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diagnosis
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Paranasal Sinus Diseases
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complications
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diagnosis
3.Clinical analysis of treatment effect on 64 patients with intractable epistaxis under nasal endoscopy and selection of nasal packing materials.
Qingjia GU ; Jingxian LI ; Jiangang FAN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(3):198-200
Adolescent
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Adult
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Aged
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Endoscopy
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Epistaxis
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surgery
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Female
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Humans
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Male
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Middle Aged
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Nasal Surgical Procedures
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Young Adult
4.Isolation and Identification of Coumarin in Dendrobium denneanum Cultivated in Sichuan and Its Quality Standard Study
Min LI ; Youping LIU ; Jingxian FAN ; Ling ZHANG ; Huiyong LI
China Pharmacy 2001;0(07):-
OBJECTIVE:To establish the quality creteria of Dendrobium denneanum cultivated in Sichuan.METHODS: The reference substance had been isolated and identified.HPLC and TLC were applied for determination and identification respectively.RESULTS: The coumarin control had been successfully isolated and the TLC spots were clear.The linear range of coumarin was 0.096~0.480 ?g with an average recovery of 99.1%(RSD=1.44%,n=6).CONCLUSION: The results can overall reflect the internal quality of D.denneanum cultivated in Sichuan and provide basis for quality evaluation and development of D.denneanum from Sichuan.
5.Study on Chemical Constitutes of Ligusticum chuanxiong and Content Determination of Ligustilide
Ling ZHANG ; Youping LIU ; Min LI ; Huiyong LI ; Jingxian FAN
China Pharmacy 2005;0(15):-
OBJECTIVE:To study the chemical constitutes of Ligusticum chuanxiong and to establish the method for the content determination of ligustilide. METHODS:The compounds were extracted and percolated by ethanol. Then the samples were separated using silica gel and identified by 1H-NMR,13C-NMR data.HPLC was used to assay the contents of ligustilide. RESULTS:Three compounds were isolated and their structures were identified as Z-ligustilide,Z-6,8',7,3'-diligustilide and Z,Z'-6,6',7,3'a-diligustilide. The contents of ligustilide were no less than 0.70%.CONCLUSION:The separation method can be used to prepare high-purity ligustilide reference substance. And the determination method can be used for quality control of L. chuanxiong.
6.The effects of isoflurane on the ratio of Bcl-2/Bax in rat fetal brain and spatial learning ability at juvenile age
Yujuan LI ; Chuiliang LIU ; Jingxian ZENG ; Yifan ZHAO
Chinese Journal of Behavioral Medicine and Brain Science 2010;19(1):4-6
Objective To determine whether fetal rats exposure to isoflurane will cause postnatal learning and memory deficits,and change Bcl-2/Bax ratio in the hippocampus CA1 and retrosplenial cortex in fetal brain of rats. Methods Twenty-eight Sprague Dawley pregnant rats at gestation day 21 (E21) were randomly divided into isoflurane treatment group(n=14) and sham control group(n=14). Rats in isoflurane treatment group were ex-posed to 1.3% isoflurane in a carrying gas of 30% oxygen, balance nitrogen for 6 h in a warmed, humidified cham-ber. For sham control group,animals were treated at the same condition with only carrying gas. In behavior study,the spatial learning and memory ability at juvenile ages was determined with the Morris Water Maze(MWM). In immunohistochemistry study,changes of Bcl-2 and Bax expression in hippocampus CA1 and retrosplenial cortex in the fetus brain after isoflurane treatment at 2 hours was performed by using immunofluorecence staining.Results In the MWM training, the escape latency to platform in the place trials showed no significant difference between the two groups,but the postnatal rats in 1.3% isoflurane group showed obviously improved retention of memory by spending more percentage of time swimming in the probe quadrant as compared to the control animals ((42.33±2.31) s vs (33.2±2.15) s, t=2.21, P<0.05) in the probe test. Compared to controls, 1.3% isoflu-rane exposure for 6 h to the pregnant rats increased the intensity of Bcl-2, decreased the intensity of Bax, and sig-nificantly increased the Bcl-2/Bax ratio in the fetal hippocampal CA1 region (4.40±0. 86 vs 1.31±0.32, t=3. 378, P<0.01) and the fetal retrosplenial cortex (5.07±1.27 vs 1.47±0.48, t=2.656, P < 0.05) respec-tively. Conclusion 1.3% isoflurane exposure in pregnant rats significantly improves the spatial retention memo-ry of their rat pups at a juvenile age and increases the Bcl-2/Bax ratio in the hippocampal CA1 region and the ret-resplenial cortex in the fetal rat brains.
7.Comparative analysis on the performance evaluation of hospitals between China and some countries
Rongqin JIANG ; Jingxian LI ; Dan HU ; Aichen GE ; Jiaying CHEN
Chinese Journal of Health Policy 2016;9(12):62-67
This study aimed at comparing the United States, Britain, Australia, the Netherlands and China’s national performance evaluation, and sum up the experience to provide a theoretical basis for the China’s development of hospital performance evaluation system. The study found that China needs to consider the patient's perspective, to establish a fixed third-party performance evaluation agencies, establish an effective incentive mechanism and feedback mechanism and combine a variety of assessment methods in the development of hospital performance evaluation index system.
8.In vitro susceptibility of Geotrichum silvicola to five antifungal agents and electron microscopic observation of terbinafine-treated Geotrichum silvicola
Lining WU ; Chen LI ; Dan WANG ; Binglan CAO ; Jingxian ZHU
Chinese Journal of Dermatology 2016;49(9):626-629
Objective To evaluate in vitro antifungal activities of five clinically common antifungal agents against Geotrichum silvicola,and to observe morphological changes of Geotrichum silvicola after treatment with terbinafine.Methods Five antifungal agents commonly used in clinic,including terbinafine (TER),amphotericin B (AmB),flucytosine (FC),fluconazol (FCZ) and itraconazole (ICZ),were tested in this study.According to the National Committee for Clinical Laboratory Standards (NCCLS) M27-A2 document and relevant literatures,a susceptibility test was performed to determine the minimal inhibitory concentration (MIC) values of the five antifungal agents against G eotrichum silvicola.Scanning electron microscopy (SEM) was used to observe morphological changes of Geotrichum silvicola treated with different concentrations of TER.Results The MIC values against Geotrichum silvicola were as follows:TER,0.01 μg/ml;AmB,0.4 μg/ml;FC,2 μg/ml;FCZ,2.69 μg/ml;ICZ,0.25 μg/ml.Geotrichum silvicola was sensitive to TER,AmB,FC and FCZ,and sensitive to ICZ in a dose-dependent manner.SEM revealed that after TER treatment,the surface of Geotrichum silvicola became rough and shrinking with irregular defects and holes,and even gave a fragment-like appearance.Conclusions Among the five tested antifungal agents,TER shows the strongest activity against Geotrichum silvicola.Within a certain range,the higher the concentration of TER,the more serious the damage to Geotrichum silvicola.
9.Osthole reduced Aβ synthesis by up-regulatingmiRNA-107 in neurons transfected with APP595/596 gene
Honghe XIAO ; Yanan JIAO ; Yuhui YAN ; Hongyan LI ; Jingxian YANG
Chinese Pharmacological Bulletin 2017;33(8):1079-1085
Aim To investigate the neuroprotective effects of osthole(Ost)on the primary cultured cortical neurons transfected with APP595/596 gene and its underlying mechanism.Methods Neonatal mouse cortical neurons were transfected with APP595/596 gene to establish AD cell models for the further study.Then,the cell viability was detected by CCK-8 assay,and the leakage of lactate dehydrogenase(LDH)was assayed by LDH kit to evaluate the injury degree.Transferase-mediated nick end labeling(TUNEL)was used to evaluate the cell apoptosis.The expression of β-amyloid peptide(Aβ)and β-site APP cleaving enzyme 1(BACE1)was measured by immunofluorescence,while the miRNA-107 was measured by RT-PCR.Results Compared to model group,Ost could significantly improve the neurons viability,decrease the LDH release and prevent the apoptosis.Ost also inhibited the expression of Aβ and BACE1 at protein level,while enhanced the expression of miRNA-107 at gene level.Conclusion Ost plays a neuroprotective role in neurons transfected with APP595/596 gene in part through up-regulating miRNA-107.
10.ESTIMATION OF NITROGEN RETENTION BY N-BALANCESTUDIES AND THE ADEQUATE PROTEIN INTAKEOF PRESCHOOL CHILDREN
Wenzhen PANG ; Eryi XU ; Shouqin LI ; Shuping CHE ; Jingxian MO
Acta Nutrimenta Sinica 1956;0(04):-
Nitrogen balance of normal developed, healthy preschool children 4-7 yrs old, lodged in the kindergartens was studied. 105 preschool children were divided into 15 groups at different protein intakes ranged from 6.28g /MJ to 10.35g/MJ. Prominant correlationship was shown between the intake of protein g/MJ (x) and the retained nitrogen g/kg (y), r= 0.6709, n= 15, p