In the teaching reform of cytobiology,we should first formulate a reasonable syllabus according to training goals,pay attention to course content reform,strengthen connection with other preclinical medicine subjects,combine quality-oriented education in class instruction,and cultivate students scientific and original spirit.The experimental courses should be offered from higher level and be conformed with subject development.The teacher should have innovative thinking,adopt diversified teaching methods,and fully arouse students' study enthusiasm.

Objective To investigate the relationship between the serum concentration of amiodarone and QT dispersion.Methods The serum concentration of Am and DEA were determined by HPLC during the treatment in forty Patients to study the relationship between the serum concentration and QTd.Results After using amiodarone,QT interval was significantly increased[(383±31)ms vs(418±29)ms,P＜0.05].In the same time QTd was significantly decreased[(34±12)ms vs(21±9)ms,P＜0.05].With maintaining dosage,the serum concentration of DEA had positive relationship with the increment of QT(r=0.50,P＜0.05).While with loading dosage,the serum concentration of amiodarone had no relationship with it.The serum concentration of amiodarone had no relationshiP with the decrement of QTd in all patients.And it varied in a large range.Between the groups of different therapeutic effect,the serum concentration had no statistical difference.Two patients appeared the adverse effects.Their serum concentration of amiodarone weren't higher than the average concentration of the group.Conclusion The serum concentration of amiodarone couldn't be an index tO estimate the effect and the occurrence of adverse effects.

Objective To probe into the application feature and use tendency of traditional Chinese medicine (TCM) injections in our hospital;To evaluate rational drug use and provide reference for related researches. Methods TCM injections in our hospital during 2010-2012 were analyzed by employing the analytic method of defined daily dose, with a purpose to evaluate frequency of drug use and rationality of medication expenses. Results From 2010 to 2012, drug possession rate of TCM injections in our hospital was 4.07%-4.47%;the amount of medication accounted for 10.50%to 12.69%of the amount of all drugs;the annual growth rate increased significantly, among which TCM injections used for removing blood stasis took the lead;frequency of drug use rose annually;medication synchrony in 3 years indicated that daily expensesof Shenkang Injection, Aidi Injection, salvianolateinjection, safflor yellow sodium chloride injection and other TCM injections were on the high side. Conclusion From 2010 to 2012, TCM injections in the clinical application in our hospital were of relatively high frequency, and the TCM injections used for removing blood stasis took the lead. Daily expenses of TCM injections drugs were on the high side and there may be unreasonable application.

A new method of the separation of hydrocarbons and glycerides in liver oil of Mustelus jriseas was designed. The Waters Prep-500A Liquid Chromatograph with silica gel column and differential refractive index detector was used. The mobile phase was petroleum ether and ethyl acetate. The pure hydrocarbons' and glycerides were obtained from the liver oil.

Tripterygium Hypoglaucum Hutch and Tripterygium Wilfordii Hook.f are all Evonymus alata family Tripterygium Wilfordii genus plants.They are generally used in the clinical treatment in department of dermatology.They resemble each other not only in some chemical constitutions,but also in some pharmacologic actions(such as anti-inflammatory,anti-tumor,immune suppression,antifertility and so on),but they are all poisonous plants.When they are used for therapy,they can induce some toxic reactions,even the death of patients.In order to facilitate the adoption of the two drugs,we review their pharmacologic actions.

As we know more and more about asthma pathogenesis and chronic inflammatory disorder mechanism,and additional immunomodulator options become available.Studies have suggested that Th2 cell differentiation increases,and Th1 cell differentiation reduces in asthma.Therefore,it becomes an important immunotherapy method to modulate the Th1/Th2 response by promoting Th1 cytokines and inhibiting Th2 cytokines.At present,the use of immunomodulator such as anti-IgE antibody,anti-IL-5 agents,CpG oligodeoxynucleotides,imiquimod,bacterial extracts,Chinese medicine in asthma has already made some progress,and besidse it demonstrate great efficacy in clinical.

Objective To investigate the effects and the safety of different dose of simvastatin on aged patients with hyperlipidemia.Method The 70 patients were divided into two groups at random. 40 in group A was given orally simvastatin 20 mg/d, and 30 patients in group B was given orally simvastatin 40 mg/d,respectively; The levels serum total cholesterol(TCH), low-density lipoprotein cholesterol(LDL-C), high-density lipoprotein cholesterol(HDL-C) and triglyceride(TG) were measured following the 4th week, the 8th week and the 12th week . And the changes of the functions of the patients'liver and kidney, the serum glucose level and the activities of myocardial enzymes in the two groups before and after the simbastatin treatment were also observed. Results In the 4th , 8th and 12th week at the treatment with simvastatin 20mg/d, the patients' TCH levels were decreased by 32 1%,32 1%,36 0% respectively;the TG levels were decreased by 35 1%,49 6%,52 9% respectively;the LDL levels were decreased by 39 3%,37 3%,35 2% respectively;and the HDL-C levels were increased by 2 0%,0 8%,8 3%, respectively. While in the 4th, 8th and 12th week at the treatment of simvastatin 40mg/d,the patients' TCH levels were decreased by 41 8%,48 0%,51 0%, TG levels were decreased by 18 4%,32 3%,38 4%, the LDL levels were decreased by 42 4%,47 5%,50 8%, and the HDL-C levels were increased by 1 0%,2 0%,13 0% respectively. There were no obviously changes of the results of the tests of the liver function,the kidney function,the blood glucose level and the myocardial enzymes before and after both treatment groups in the different dose of the simvastain. Conclusion The effects and the safety of the treatments of simvastatin on aged patients with hyperlipidemia were similar at the dose of 20mg/d and the dose of 40mg/d.

Primary nephrotic syndrome(PNS) is a common kidney disease. However,its mechanisms remain unclear,and immunity might to play an important role in PNS. This article will review the pathology from cellular immunity,humoral immunity and the immunity involved in podocytes. It is useful for further understand-ing,and it may help guide the diagnosis,prognosis and therapeutic strategies.

Long-term care is to be one of the issues of common concern to the older persons,with the development process of China's aging population,the older persons with disabilities will become the major groups in the number of disabled population.They need to get more attention by society as they become a class of special groups.Domestic and foreign scholars have conducted a lot of research on the longterm care,the relevant progress are summarized below.

(-)-Epigallocatechin-3-gallate (EGCG),a natural medicine with anti-neoplastic activity,significantly prevent cancers via inhibiting tumor cell proliferation,suppressing angiogenesis and promoting apoptosis,etc.Furthermore,the new discoveries about anti-tumor mechanisms of EGCG in digestive system,reproductive system and respiratory system shorten the course of EGCG application in clinical.