In the teaching reform of cytobiology,we should first formulate a reasonable syllabus according to training goals,pay attention to course content reform,strengthen connection with other preclinical medicine subjects,combine quality-oriented education in class instruction,and cultivate students scientific and original spirit.The experimental courses should be offered from higher level and be conformed with subject development.The teacher should have innovative thinking,adopt diversified teaching methods,and fully arouse students' study enthusiasm.

Objective To probe into the application feature and use tendency of traditional Chinese medicine (TCM) injections in our hospital;To evaluate rational drug use and provide reference for related researches. Methods TCM injections in our hospital during 2010-2012 were analyzed by employing the analytic method of defined daily dose, with a purpose to evaluate frequency of drug use and rationality of medication expenses. Results From 2010 to 2012, drug possession rate of TCM injections in our hospital was 4.07%-4.47%;the amount of medication accounted for 10.50%to 12.69%of the amount of all drugs;the annual growth rate increased significantly, among which TCM injections used for removing blood stasis took the lead;frequency of drug use rose annually;medication synchrony in 3 years indicated that daily expensesof Shenkang Injection, Aidi Injection, salvianolateinjection, safflor yellow sodium chloride injection and other TCM injections were on the high side. Conclusion From 2010 to 2012, TCM injections in the clinical application in our hospital were of relatively high frequency, and the TCM injections used for removing blood stasis took the lead. Daily expenses of TCM injections drugs were on the high side and there may be unreasonable application.

A new method of the separation of hydrocarbons and glycerides in liver oil of Mustelus jriseas was designed. The Waters Prep-500A Liquid Chromatograph with silica gel column and differential refractive index detector was used. The mobile phase was petroleum ether and ethyl acetate. The pure hydrocarbons' and glycerides were obtained from the liver oil.

Objective To investigate the relationship between the serum concentration of amiodarone and QT dispersion.Methods The serum concentration of Am and DEA were determined by HPLC during the treatment in forty Patients to study the relationship between the serum concentration and QTd.Results After using amiodarone,QT interval was significantly increased[(383±31)ms vs(418±29)ms,P＜0.05].In the same time QTd was significantly decreased[(34±12)ms vs(21±9)ms,P＜0.05].With maintaining dosage,the serum concentration of DEA had positive relationship with the increment of QT(r=0.50,P＜0.05).While with loading dosage,the serum concentration of amiodarone had no relationship with it.The serum concentration of amiodarone had no relationshiP with the decrement of QTd in all patients.And it varied in a large range.Between the groups of different therapeutic effect,the serum concentration had no statistical difference.Two patients appeared the adverse effects.Their serum concentration of amiodarone weren't higher than the average concentration of the group.Conclusion The serum concentration of amiodarone couldn't be an index tO estimate the effect and the occurrence of adverse effects.

Tripterygium Hypoglaucum Hutch and Tripterygium Wilfordii Hook.f are all Evonymus alata family Tripterygium Wilfordii genus plants.They are generally used in the clinical treatment in department of dermatology.They resemble each other not only in some chemical constitutions,but also in some pharmacologic actions(such as anti-inflammatory,anti-tumor,immune suppression,antifertility and so on),but they are all poisonous plants.When they are used for therapy,they can induce some toxic reactions,even the death of patients.In order to facilitate the adoption of the two drugs,we review their pharmacologic actions.

Objective To study the role of bacterial collagenase in wound healing with observing capillary neovascularization, epidermis regeneration and collagen fiber reparation in vivo. Method A total of 28 New Zealand rabbits were made into models of superficial soft tissue trauma and divided into two groups. In the experimental group, the right limbs of rabbits were traumatized intentionally in the same length and depth, and their wounds were treated with bacterial collagenase liquor 133 MIU/mL, and the wounds were sutured. Similarly, in the control group, the left limbs of the rabbits were modeled in the same way and their wounds were managed with physiological saline instead and closed. Their wounds were observed continually 1, 2, 3, 5, 7, 10 and 14 days after traumatized. Data were analyzed statistically by using One-way ANOVA, LSD-test, and Wilcoxon rank test. Results There were no significant differences in capillary neovascularization and epidermis regeneration between two groups ( F = 0.12, P = 0.740 and F = 0.95, P = 0.33, respectively), but significant difference in collagen fiber reparation was found between two groups (F = 6.63, P = 0.01). Conclusions In the progress of wound healing, bacterial collagenase can improve the collagen fiber reparation without effects on the new capillary vasculogenesis and epidermis regeneration. The bacterial collagenase does not play a positive role in wound healing.

Objective To analyse status of peripheral blood leukemic cells detected by flow cytometry in patients with acute leu‐kemia(AL) ,and to provide references for evaluating clinical efficacy and prognosis of AL .Methods The peripheral blood specimens of 87 cases of patients with AL ,including 53 cases of patients with acute myelocytic leukemia and 34 cases of patients with acute lymphoblastic leukemia ,were detected by using flow cytometry ,morphological changes in bone marrow cells were detected ,as well . Results The sensitivity ,specificity and positive predictive value in determination of acute myelocytic leukemia was 95 .6% ,34 .5%and 81 .3% respectively ,and those in acute lymphoblastic leukemia was 87 .3% ,45 .6% and 68 .9% respectively ,statistically signif‐icant differences were found in sensitivity ,specificity and positive predictive value (P<0 .05) .A total of 19 cases with negative mini‐mal residual disease had recurrence(26 .31% ) after 24 months ,and 68 cases with positive minimal residual disease had recurrence (86 .76% ) after 24 months ,and the recurrence rate between the two groups was statistically significant (P<0 .05) .Among all pa‐tients with positive minimal residual disease ,the recurrence rate in patients with high expression level of minimal residual disease (88 .23% ) was higher than that in patients with low expression level of minimal residual disease (47 .09% ) ,and the difference was statistically significant (P<0 .05) .Conclusion Flow cytometric analysis of peripheral blood leukemic cells may has significance for diagnosing relapse of AL and guiding clinical medication .

Objective To evaluate the therapeutic efficacy of the comprehensive auricular point therapy in treating post-stroke urinary incontinence. Method A hundred patients with post-stoke urinary incontinence were randomized into a treatment group and a control group, 50 in each group. The treatment group was intervened by comprehensive auricular point therapy, while the control group was by rehabilitation training. The therapeutic efficacies were evaluated after 2 treatment courses. Result The total effective rate was 82.0%in the treatment group versus 62.0%in the control group, and the difference was statistically insignificant (P>0.05). Conclusion Comprehensive auricular point therapy is effective in treating post-stroke urinary incontinence.

Functional dyspepsia( FD)is a common functional gastrointestinal disorder,its pathogenesis has not been fully clarified. Recent studies showed that gastrointestinal mucosal low-grade inflammation had been found in patients with FD,and impaired mucosal integrity,psychological factor and abnormal brain-gut axis may promote the inflammation formation,which may also contribute to the onset and persistence of FD. This article reviewed the advances in study on gastrointestinal mucosal low-grade inflammation in FD.

OBJECTIVE: To discuss the countermeasures in enforcing medical intellectual property rights strategy in China. METHODS: The urgency and status quo of enforcing medical intellectual property rights strategy in China were analyzed, and the corresponding countermeasures were put forward. RESULTS & CONCLUSION: Speed up the enforcement of medical intellectual property rights strategy is the only way toward developing medicine economy and improving our international competition edge.