Eighty patients with acute exacerbation of chronic obstructive pulmonary disease(COPD)complicated by pulmonary artery hypertention(PAH)were recruited in the study. All patients took conventional western medicine, among them 40 also received injection of Chinese herb medicine Shenxiong(study group)for 14 days. Mean pulmonary artery pressure(mPAP), plasma platelet alphagranule membrane protein(GMP-140), thromboxane B2(TXB2)and 6-keto-prostaglandin F1α(6-K-PGF1α)were measured before and after treatment in both groups. Compared with pretreatment levels and levels in control group mPAP, GMP-140 and TXB2 levels in study group were significantly decreased, while the levels of 6-K-PGF1α were significantly increased. The results indicate that Shenxiong injection may inhibit platelet activation and reduce mPAP.

Objective To evaluate the clinical and pathological features of drug-associated acute interstitial nephritis. Methods Clinical presentations and pathological features were investigated in 14 patients with drug-associated acute interstitial nephritis. Lymphocyt; stimulation test was performed to confirm, the offending drugs. Results All patients presented with acute renal failure, but typical clinical features were often absent. Renal biopsy was therefore needed to establish the diagnosis. Conclusion Lymphocyte stimulation test is a very useful means in the determination of offending drugs.

Objective To study the inclusion effect of isoandrographolide-?-cyclodextrin by method of UV,IR,and 1H-NMR.Methods The inclusion compound of isoandrographolide-?-cyclodextrin was prepared by saturated solution method assist microwave,equilibrium constants of inclusion complexes and molar ratio of isoandrogapholide-?-cyclodextrin were determined by the UV.The inclusion compound and their molar ratio were confirmed by IR and 1H-NMR.Results The isoandrographolide and ?-cyclodextrin formed inclusion compound.The equilibrium constants of inclusion complexes were 140.845 L/mol and the molar ratio was 1∶1.Conclusion The isoandrographolide could be inclused by ?-cyclodextrin and the inclusion effect is better.

The celiac ganglia from 9 mature guinea pigs of both sexes were fixed in a mixture of 2％ glutaraldehyde and 2.5％ depolymerized paraformaldehyde. The SIF cells in cryostat sections were discerned out through their eatecholamine fluorescence induced by the fixative. These cells were processed through routine procedures for electron microscopy after or without incubation for acid phosphatase (AcP). The ultrastructure and AcP activity of these cells were observed under an H-600 electron microscope.The SIF cells were found to be present in clusters adjecent to fenestrated capillaries. The exocytosis of vesicular granules of SIF cells were seen on the surface of their cell membrane facing the capillaries. In addition, some dark SIF cells which were more osmiophilic and rich in mitochondria were discovered in this ganglion. These ultrastructural features of the SIF cells indicate that they may perform functions of an endocrine and/or paracrine nature.After one. hour incubation in a Gomori-typed medium at 37℃, some granules. besides the typical lysosomes display the AcP activity. Although the nature of the AcP-positive granules hasn't been elucidated a speculation is laid on that the presence of the enzyme on these granules may play a role of regulation in replacing the intragranular contents.

Monoamine oxidase and thiamine pyrophosphatase activities were demonstrated ultracytochemically in the celiac ganglion and adrenal medulla of the guinea pig by Shannon's and Novikoff's method respectively. Monoamine oxidase activity was found frequently on the nuclear evelope, and ocassionally in mitochondrial outer compartment and cell membrane of the small intensely fluorescent (SIF) cells of the ganglion. Similar Iocalizations were also observed in chromaffin cells of the adrenal medulla. In pricipal neurons of the ganglion, a large amount of products of the monoamine oxidase reaction were found on the endoplasmic reticulium in addition to the nuclear envelope and mitochondrial membranes. Abundant thiaminepyrophosphatase activity was seen at the tran-face of the Golgi complex in the pricipal neurons, in contrast to which, both SIF cells and chromaffin cells exhibited little thiamine pyrophosphatase activity. The results suggested that catecholamine metabolism and the mode of functional activity in the SIF cells were different from those in the pricipal neurons but similar to those in the chromaffin cells of adrenal medulla.

Serial cryostat sections of the celiac ganglion were prepared from 30 guinea pigs.The process of De Da Torre's glyoxylic acid method and different histochmical rea-ctions were performed consecutively on the same section.Under the conditions employ-ed in this experiment,it was shown that the SIF cells,in contrast to the principalneurons,exhibited a more intense reation of adenosine triphosphatase,and a lessintense reaction of monoamine oxidase,lactic dehydrogenase and nonspecific esterase,and a negative reaction of alkaline phosphatase and acetylcholinesterase.The acidphosphatase reaction of most of SIF cells was weak as demonstrated with both theGomori's lead method and the simultaneously coupling method.The SIF cells showedless lipids,nucleic acids and about the same content in tyrosin as compared with theprincipal neurons.These histochemical properties of SIF cells,as well as their morphological charac-teristics of grouping in clusters and surrounding blood vessels,implicated that theyfunction in a different way from that of principal neurons,and probably as endocrineand/or paracrine cells in modulating nervous transmission in the celiac ganglion.

Stereological methods were applied in the qantitative ultrastructural analysis of the chromaffin cells in adrenal medulla of normal adult guinea pigs.The main result includes:(1)The average volume(V)of each cell(714?m~3)and its nucleus (167?m~3);(2)volume density(Vv)of mitochondria(0.082?m~3/?m~3),lysosomes (0.0045?m~3/?m~3),rough-endoplasmic reticulium(0.013?m~3/?m~3),smooth-endopas- mic reticulium including Golgi apparatus(0.024?m~3/?m~3)and granule vesicles (0.23?m~3/?m~3);(3)surface density(Sv)of cell membrane(0.87/?m~2/?m~3)and mitochondrial outer membrane(0.90?m~2/?m~3);(4)numerical density(Nv)of mitochondria(0.89/?m~3),lysosomes(0.11/?m~3)and granule vesicles(59.98/?m~3); (5)the mean diameter of granule vesicles(144 nm),In addition,several small- granule chromaffin cells were quantified separately from the general chromaffin cells.They contain granule vesicles with an average diameter of 97 nm and show a significant difference in surface density of cell membrane(1.54?m~2/?m~3)from that relevant value of general chromaffin cells(P

Objective To evaluate the expression of immunohistochemistry markers p53, p16 and Ki-67 in penile cancer and penile intraepithelial neoplasia (PIN). Methods 33 patients who suffered penile cancer and received organ-sparing surgery from Jan 2004 to Dec 2013 were retrospectively analysed. The expression of p53, p16 and Ki-67 were detected by EnVision/HRP two steps immunohistochemistry. Results The positive expression rates of p53 in normal penile epithelium, low-grate penile intraepithelial neoplasia, high-grate penile intraepithelial neoplasia, well-differentiated squamous cell carcinoma and poor-differentiated squamous cell carcinoma were 0, 16.7 %, 38.5 %, 30.0 % and 66.7 % respectively. The expression rates of p16 were 20.0 %, 50.0 %, 53.8 %, 70.0 % and 33.3 % respectively. The expression rates of Ki-67 were 0, 16.7 %, 61.5 %, 65.0 % and 75.0 % respectively. Conclusions The combined detection of p16 and Ki-67 can contribute to discover PIN and penile carcinoma much more earlier. p53 may be related to the occurrence and development of low differentiated squamous cell carcinoma, and p16 may be related to the occurrence and development of well-differentiated squamous cell carcinoma.

Bone Diseases ; chemically induced ; epidemiology ; China ; epidemiology ; Chronic Disease ; Fluoride Poisoning ; epidemiology ; etiology ; genetics ; Fluorosis, Dental ; epidemiology ; etiology ; Humans ; Prevalence

Humans ; Metabolic Syndrome