Eighty patients with acute exacerbation of chronic obstructive pulmonary disease(COPD)complicated by pulmonary artery hypertention(PAH)were recruited in the study. All patients took conventional western medicine, among them 40 also received injection of Chinese herb medicine Shenxiong(study group)for 14 days. Mean pulmonary artery pressure(mPAP), plasma platelet alphagranule membrane protein(GMP-140), thromboxane B2(TXB2)and 6-keto-prostaglandin F1α(6-K-PGF1α)were measured before and after treatment in both groups. Compared with pretreatment levels and levels in control group mPAP, GMP-140 and TXB2 levels in study group were significantly decreased, while the levels of 6-K-PGF1α were significantly increased. The results indicate that Shenxiong injection may inhibit platelet activation and reduce mPAP.

Humans ; Metabolic Syndrome

The celiac ganglia from 9 mature guinea pigs of both sexes were fixed in a mixture of 2％ glutaraldehyde and 2.5％ depolymerized paraformaldehyde. The SIF cells in cryostat sections were discerned out through their eatecholamine fluorescence induced by the fixative. These cells were processed through routine procedures for electron microscopy after or without incubation for acid phosphatase (AcP). The ultrastructure and AcP activity of these cells were observed under an H-600 electron microscope.The SIF cells were found to be present in clusters adjecent to fenestrated capillaries. The exocytosis of vesicular granules of SIF cells were seen on the surface of their cell membrane facing the capillaries. In addition, some dark SIF cells which were more osmiophilic and rich in mitochondria were discovered in this ganglion. These ultrastructural features of the SIF cells indicate that they may perform functions of an endocrine and/or paracrine nature.After one. hour incubation in a Gomori-typed medium at 37℃, some granules. besides the typical lysosomes display the AcP activity. Although the nature of the AcP-positive granules hasn't been elucidated a speculation is laid on that the presence of the enzyme on these granules may play a role of regulation in replacing the intragranular contents.

Monoamine oxidase and thiamine pyrophosphatase activities were demonstrated ultracytochemically in the celiac ganglion and adrenal medulla of the guinea pig by Shannon's and Novikoff's method respectively. Monoamine oxidase activity was found frequently on the nuclear evelope, and ocassionally in mitochondrial outer compartment and cell membrane of the small intensely fluorescent (SIF) cells of the ganglion. Similar Iocalizations were also observed in chromaffin cells of the adrenal medulla. In pricipal neurons of the ganglion, a large amount of products of the monoamine oxidase reaction were found on the endoplasmic reticulium in addition to the nuclear envelope and mitochondrial membranes. Abundant thiaminepyrophosphatase activity was seen at the tran-face of the Golgi complex in the pricipal neurons, in contrast to which, both SIF cells and chromaffin cells exhibited little thiamine pyrophosphatase activity. The results suggested that catecholamine metabolism and the mode of functional activity in the SIF cells were different from those in the pricipal neurons but similar to those in the chromaffin cells of adrenal medulla.

Objective To explore the effect of oxidative stress on intermittent hypoxia induced-hip-pocampal injury in rats. Methods 30 adult male Sprague-Dawley rats were random divided into three groups ( 10 rats in each group), control group( CON group), intermittent group( IH group), and melatonin group( MEL group). The levels of MDA and SOD were detected by colorimetric method, and RT-PCR was used to examine the mRNA levels of the Cu/ZnSOD, GPx, CAT in hippocampal tissues. Results The level of MDA in IH group was ( 1. 68 ±0. 23) μmol/g, and it was obviously higher than that in control group (1.25±0.14)μmol/g and MEL group(1.35 ±0.18) μmoL/g ( P ＜0.05, P ＜0.01). In IH group, the activity of SOD and the mRNA levels of the Cu/ZnSOD,GPx and CAT were 43.01 ±4. 96 103NU/g, 0.25±0. 02,0. 34 ±0. 09,0. 38 ±0. 03 respectively, which were significantly lower than those in control group(61.12 ±5.68 103NU/g protein,0. 48 ±0.06,0. 55±0.07,0.57 ±0.04) and MEL group (55.98 ±4.65 103 NU/g,0.43 ± 0.08,0.54 ± 0.05,0.53 ± 0.07 ) ( P ＜ 0.05, P ＜ 0. 01 ). Conclusion Intermittent hypoxia can induce hippocampal injury in rats by oxidative stress, and melatonin can inhibit intermittent hypoxia induced-oxidant stress, so it can protect intermittent hypoxia induced-hippocampal injury in rats.

Objective To evaluate the value of serum CA 19-9 levels in predicting resectability of pancreatic cancer. Methods Serum CA 19-9 levels of 52 patients with pancreatic cancer who were possible to be curatively resected according to the imaging were determined; the gold standard was respectability. An receiver operating characteristic (ROC) curve was constructed for the CA 19-9 levels; the point that lies closest to the upper left-hand comer of the graph is chosen as the cut-off point, the sensitivity, specificity,positive and negative predictive values were calculated. Results There were 29 (55.8%) resectable and 23 (44.2%) inoperable cases of pancreatic cancers. The CA19-9 level in resectable patients was (159.6±170.9)U/ml, and it was (944.4 ±773.4) U/ml in inoperable cases. The area under the ROC curve was 0. 918 ( ＞ 0.9, P ＜ 0.01 ), 95% confidence interval were 0.843 ～ 0.992. The CA19-9 level was (353.2) U/ml according to the cut-off point, the sensitivity and specificity was 93.1% and 78.3%, respectively; positive and negative predictive values was 84. 4% and 90. 0% , respectively. Conclusions Preoperative serum CA19-9 level may be a useful marker for further evaluating the respectability of pancreatic cancer who are possible to be resected according to the imaging studies.

Objective To investigate the distribution of authors,funding,institutions and regions of Chinese Journal of Orthopaedics between 2010 and 2015,understand the types and characteristics of authors＇ cooperation,identify the core author,core institutions and core regions,as well as evaluate academic level and journal quality from the view of literature citation.Methods According to Chinese Science Citation Database (referred CSCD) and bibliometric methods,we made statistical analysis,including the number of papers,cooperation,core authors,regional distribution of authors,systematic distribution of authors,productive institutions,funding sources and distribution.On the basis of Chinese science and technology journal citation reports (Compendex) from 2013 to 2015,the main indicators and coupling relationship of bone surgery journals were evaluated.Results From 2010 to 2015,Chinese Journal of Orthopaedics published 1 342 papers.There were 1 009 first authors,among which 802 authors (79.48％,802/1 009) published only one paper; 1 293 co-author papers contributed to 96.35％ cooperation rate and 5.69 degree of cooperation.Forty-nine papers (3.65％,49/1 342) had only one author,and 799 papers (59.54％,799/1 342) were published by affiliated Hospital,321 papers (23.92％,321/1 342) by provincial and municipal hospitals,138 papers (10.28％,138/1 342) by military hospital.There were 63 (6.24％,63/1 009) core authors publishing 252 papers (18.78％,252/1 342),and 20 productive institution publishing 630 papers (46.94％,630/1 342) in which Beijing was ranked at No.1 with 238 papers (17.73％,238/1 342).From 2010 to 2015,Chinese Journal of Orthopaedics publishing papers got 652 funding.From 2012 to 2014,its core total citations were 10 153,ranked No.1 in bone surgery journals in 2012 and ranked No.2 in 2013-2014; impact factors rose from ranked No.4 in 2012 to ranked No.2 in 2014,and the comprehensive evaluation of total score ranked No.1 in 2012-2014.Conclusion Chinese Journal of Orthopaedics has a strong team with a large number of authors,extensive source of institutions,a wide regional distribution,high cooperation rate,and strong ability to attract assistance funded paper.Affiliated Hospital and provincial and municipal hospitals are the main source of paper in the journal.Its core total citations,impact factor,subject diffusion index,academic impact indicators,comprehensive evaluation score were located in front rank of the same field of journals.

Objective To evaluate the expression of immunohistochemistry markers p53, p16 and Ki-67 in penile cancer and penile intraepithelial neoplasia (PIN). Methods 33 patients who suffered penile cancer and received organ-sparing surgery from Jan 2004 to Dec 2013 were retrospectively analysed. The expression of p53, p16 and Ki-67 were detected by EnVision/HRP two steps immunohistochemistry. Results The positive expression rates of p53 in normal penile epithelium, low-grate penile intraepithelial neoplasia, high-grate penile intraepithelial neoplasia, well-differentiated squamous cell carcinoma and poor-differentiated squamous cell carcinoma were 0, 16.7 %, 38.5 %, 30.0 % and 66.7 % respectively. The expression rates of p16 were 20.0 %, 50.0 %, 53.8 %, 70.0 % and 33.3 % respectively. The expression rates of Ki-67 were 0, 16.7 %, 61.5 %, 65.0 % and 75.0 % respectively. Conclusions The combined detection of p16 and Ki-67 can contribute to discover PIN and penile carcinoma much more earlier. p53 may be related to the occurrence and development of low differentiated squamous cell carcinoma, and p16 may be related to the occurrence and development of well-differentiated squamous cell carcinoma.

Objective To determine the levels of radiation-induced expressions of HIF-1α and Survivin in three human lymphoma cell lines with or without HIF-1a specific antagonist or HIF-1a siRNA transfection and to explore the possible mechanism of cell radioresistance.Methods Namalwa,Ramos,Raji cells were irradiated with X-rays with or without the combination of echinomycin (EC),a HIF-1α specific inhibitor,or transfection of HIF-1α siRNA.Then the expression levels of HIF-1α and Survivin proteins and mRNAs were determined by Western blot and real-time PCR,respectively.Results After irradiation,the expression level of HIF-1α protein was up-regulated at 10-20 h followed by up-regulation of survivin protein at 24 h (t =7.53-31.31,P ＜ 0.01 ) in three lymphoma cell lines.Radiation-induced up-expression of survivin protein was significantly reduced by either HIF-1α inhibition or EC treatment (t =7.21-32.81,P ＜0.01 ).However,radiation failed to induce up-erpression of survivin at both mRNA and protein levels after transfection with HIF-1α siRNA.Conclusion HIF-1α-Survivin pathway is activated in human lymphoma cells after radiation,which might be involved in the cell radioresistance.

Plasma levels of homocysteine (Hcy),folic acid (FA) and Vitamin B12 (VB12) were measured in 120 patients of colon adenomatous polyps and 40 healthy subjects.Plasma Hcy levels in colon adenomatous polyps patients were significantly higher than those in controls[(14.24 ± 0.52) μmol/L vs.(10.93 ± 0.81) μmol/L,P =0.0014)],while plasma FA levels in polyps patients were lower than those in controls [(6.26 ± 0.27) μg/L vs.(10.74 ± 0.85) μg/L,P ＜ 0.0001].There was no difference in plasma VB12 levels between two groups(P =0.7100).The plasma Hcy levels in patients with multiple colon polyps were higher than those with single colon polyps (P =0.0092),while the plasma FA levels demonstrated contrary results (P =0.0047).With the pathological type progression,the plasma Hcy levels were gradually raised,and FA levels gradually decreased.Plasma levels of FA,Hcy and VB12 were not correlated with the location and distribution of polyps.These results suggest that plasma FA and Hcy levels may be related to disease progress in patients with colon adenomatous polyps.