OBJECTIVE:To discuss the developing strategy of pharmaceutical enterprises run by private citizens in China.METHODS:Based on China’s realities,the confronting problems in pharmaceutical enterprises run by private citizens in China were analyzed.RESULTS&CONCLUSION:The privately running enterprises should take advantages of flexible or?ganization to strengthen innovation capacity,to achieve scale management with capital operation and to fasten the establishment of mordern product circulation.

Objective To observe the efficacy of advanced non-small cell lung cancer patients treated with CIK cell combined with chemotherapy.Methods65 cases of advanced lung cancer patients admitted in our hospital from February 2013 to January 2015 were retrospectively analyzed.The patients were randomly divided into observation group (34 cases) and control group (n=31).The levels of vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9) and carcinoembryonic antigen (CEA) in the two groups were observed before and after treatment.The patients in the control group were treated with chemotherapy on the basis of chemotherapy.Cytokeratin 19 (CYFRA21-1) levels, and clinical efficacy.Results①The patients with VEGF and MMP-9 values after treatment (298.67±36.49)ng/L, (1102.31±86.48)ng/mL, than the control group (459.19±45.28)ng/L, (1394.83±121.06)ng/mL, and the difference was significant (P<0.05).②CEA and CYFR21-1 value patients in the observation group were (14.16±1.08) ng/mL, (4.45±0.45) ng/mL, than the control group (17.86±1.84) ng/mL, (5.76±0.64) ng/mL, and the difference was significant (P<0.05).③The total observation group was significantly higher than patients 94.18% 74.19% efficiency, and the difference was statistically significant (P<0.05).ConclusionCIK cell biological therapy combined with chemotherapy for advanced lung cancer patients with a certain improvement is worthy of further research and application.

Objective To analyse clinical efficacy of Fangfengtongsheng powder combined with Flupentixol and Melitracen tablets in treatment of anorectal neurosis.Methods92 patients with anorectal neurosis from April 2014 to June 2016 were grouped two groups randomly, each with 46 cases.Two groups were treated with Flupentixol and Melitracen tablets and observation group was treated another with Fangfengtongsheng powder.Clinical efficacy of two groups were compared.Results①Total effective rate of observation group was 95.65%,higher than that of control group 78.26% (P<0.05).②After treatment,SAS and SDS score of observation group were lower than that of control group(P<0.05).③After treatment, anal bulge, anal pain, frequent defecation, anal foreign body sensation score and 8 dimensions of SF-36 scale score of observation group were better than that of control group(P<0.05).④The control group had 8.70% adverse reactions, compared with observation group 4.35%, the difference was not statistically significant.ConclusionFangfengtongsheng powder can improve the therapeutic effect of anorectal neurosis on the basis of Flupentixol and Melitracen tablets, relieve the anxiety level and improve the living standard of patients.

OBJECTIVE:To provide information for future development of pharmaceutical industry.METHODS:The pay output rates of pharmaceutical industry were compared between China and America as well as Japan.RESULTS:The present situation and relative position of pay output in Chinese pharmaceutical industry were made clear.CONCLUSION:According to the pay comparative analysis result,we should raise technology structure,product structure and industry structure.On the basis of that,we can get long-term advantage.

BACKGROUND: Atrial natriuretic peptide (ANP)-synthesizing cells distribute in all over the body. However, very little is known about the morphological localization and the distribution characteristics of ANP-synthesizing cells in different regions of rat stomach. OBJECTIVE: To investigate the ultrastructural localization and distribution characteristics of ANP-synthesizing cells in rat stomach. DESIGN: Repeated measurement experiment. SETTING: Chengde Medical Collage, Chengde, Hebei Province, China. MATERIALS: This study was performed at the Laboratory for Institute of Chinese Materia Medica Immunology (provincial key laboratory), Chengde Medical College between October 2004 and July 2007. Eighteen adult Wistar rats of either gender, weighing 175-300 g, were provided by the Laboratory Animal Center of Chengde Medical College. The disposal of animals was conducted in accordance with ethical guidelines for the use and care of animals. ANP antibody and serum (Santa Cruz Biotechnology, Inc, USA), Protein A-15 nm colloidal gold labeled (Sino-American Biotechnology Company, China), CMIAS image analysis system (Beijing University of Aeronautics and Astronautics, China) and JEM-1200EX transmission election microscope (Japan) were used in the study. METHODS: After gastric and right atrial tissues (as a positive control in immanohistochemical staining for ANP-synthesizing cells) were fleshly excised from anesthetized Wistar rats, the specimens were longitudinally harvested along rat gastric cardiac region, gastric fundic mucosa and gastric pyloric region. Gastric tissue was performed immunohistochemical staining (for positive control) together with right atrial tissue, to observe the distribution characteristics of ANP-synthesizing cells in different regions of rat gastric tissue, and to localize ANP-synthesizing cells. The ultrastructural localization of ANP-synthesizing cells in the rat gastric mucosa was performed by postembedding immunoelectron microscopy. The area-density percentage of ANP-synthesizing cells in the different regions of rat stomach (gastric cardiac region, pyloric region and fundic region) was calculated with a CMIAS image analysis system. MAIN OUTCOME MEASURES: Morphological appearance and localization of ANP-synthesizing cells as well as the different area-density percentage in different regions of rat stomach. RESULTS: As a positive control, ANP-synthesizing cells showed intense positive expression in atrial myocytes and cytoplasm and in gastric mucosa (mainly within the lower third of rat gastric mucosa). The morphological appearance of the individual ANP-synthesizing cell was variable, exhibiting round, pyramidal and flask shapes. Negative staining for ANP-synthesizing cells was detected in the lamina propria, submucosa, and smooth muscle. ANP-synthesizing cells existed in different regions of rat stomach and its area-density was the largest in the pyloric region. The distribution order of ANP-synthesizing cells in area-density was gastric cardiac region > gastric pyloric region > gastric fundic mucosa in mucosa layer. CONCLUSION: ANP-synthesizing cells mainly exist within the lower third of the rat gastric mucosa. The percentage of area-density of ANP-synthesizing cellswas variable in different regions of rat gastric mucosa, and its percentage of area-density was the largest in the gastric cardiac region.

Objective To compare the effect of establishment of Coronary angiography model in minipigs with right femoral Judkins catheter and 5F universal catheter .Methods Ninteen minipigs were randomized into two groups with right femoral Judkins catheter (ten minipigs)and 5F universal catheter(nine minipigs). Success rate,operation time,X-ray irradiation time were observed .Results The success rate, operation time ,X-ray irradiation time after Coronary angiography in right femoral Judkins catheter group was 100%,(5.68 ±3.53 ) min,(20.84 ±2.45 ) min,in 5F universal catheter group was 88.8%,(6.98 ± 4.13 )min,(26.23 ±8.45 )min.This difference was signifficant( P <0.01).Conclusion Coronary an-giography in minipigs with right femoral Judkins catheter has the advantage of high success rate , less opera-tion time, X-ray irradiation time , is more suit to coronay anatomy of the minipig .

Ischemic cerebrovascular disease(ICVD) is temporary or permanent reduction of blood flow of the main arteries of the brain caused by cerebral transient,sudden,reversible neurological dysfunction.Timely recovery of blood supply to the brain is the most important way to save the patient's life,but at the same time gave restore blood perfusion brain tissue organs brought new damage,which is cerebeal ischemia reperfusion injury(CIRI).How to build an ideal,efficient,easy to operate brain ischemia-reperfusion injury in animal models to simulate the clinical study,and how to improve and innovate this model by researchers in recent years were reviewed in this article.

Objective:To explore the clinical application value of multi-slice spiral CT in the diagnosis.Methods: Twenty nine patients with clinically suspected rib fractures and X-ray showed no 64 row helical CT scanning in patients with chest trauma fractures after reconstruction, multiplanar reconstruction and VR imaging.Results: Twenty nine patients with occult fracture of rib on the MPR, VR imaging were rib image good, 28 cases were single, multiple rib fractures in 1 cases, the total of 4 cases with rib fracture, incomplete fracture in 25 cases.Conclusion: The 64 row helical CT for occult rib fracture detection rates, diagnostic accordance rate was superior to X-ray plain film, various post-processing techniques applied to timely, accurate, intuitive display rib fracture location, shape, extent of chest trauma, occult rib fracture first choice inspection method.

Objective:To investigate effect of curcumin on serum gelatinases level in patients with unstable angina pectoris (UAP). Methods:A total of 80 UAP patients admitted from January 2010 to September 2010 were enrolled and randomly divided into curcumin treatment group (n=40) and routine treatment control group (n=40). Serum levels of gelatinases [contain matrix metalloproteinase (MMP)-2 and -9] of the two groups were measured before and 30d after treatment.Results: Compared with before treatment , after treatment, serum levels of gelatinases significantly decreased [MMP-2:(52.64±6.77)ng/ml vs.(32.65±1.67)ng/ml,MMP-9:(56.75±7.34)ng/ml vs.(35±1.88)ng/ml]in curcumin treatment group (P<0.01), and they were significantly lower than those of routine treatment control group[MMP-2:(32.65±1.67)ng/ml vs.(37.78±2.76)ng/ml,MMP9:(35±1.88)ng/ml vs.(40.23±1.95)ng/ml]],P<0.05. Conclusion:Curcumin could decrease serum levels of gelatinases in patients with unstable angina pectoris, and possesses effect stabilizing coronary artery plaque.

Neural and brain imaging has become one of the most challenging subjects and attracted more attentions. Neural and brain imaging can quantify morphological pattern, structure and function of brain and nerve system, which offers us not only a deeper understanding of brain and nerve system, but also improved effectiveness of clinical diagnosis and treatment of diseases. Optical Coherence Tomography (OCT) is a new imaging technique and has been widely used in areas of biology and medicine. Study on OCT technique applying to neural and brain imaging has drawn special attention and rapid developments have been achieved. The technique provides new ideas and methods to solve problems in neural and brain imaging and its potential needs to be discovered. This article re-views the latest techniques and development in OCT for neural and brain imaging. Advantages and disadvantages of the technique and foreground of the development are discussed.