Objective To investigate the expressions of metalloproteinase-9(MMP-9)in serum,brain and aorta matrix and tissue inhibitor of metalloproteinase-1(TIMP-1) in renovascular hypertensive rats(RHR),and to evaluate the association between blood pressure and levels of matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metalloproteinase-1(TIMP-1).Methods Eighty healthy male SD rats were randomly divided into RHR group(n=40) and sham-operated group(n=40).Hypertension was induced by two-kidney,two-clip(2K-2C)clamps.Systolic blood pressure(SBP) was measured every 2 weeks during 12 weeks using a tail pressure meter.Stroke was confirmed by Longa's five-point scale and pathological examination.The expressions of MMP-9 and TIMP-1 in the brain and aorta tissues were detected by Western blot and immunohistochemistry.The levels of serum MMP-9 and TIMP-1 were measured by double-antibody sandwich enzyme-linked immunosorbent assay(DAS-ELISA).Results Compared with the sham-operated group,SBP stayed significantly elevated in the RHR group at 2,4,6,8,10 and 12weeks after the operation [(157±9.0) vs.(128±7.0),(176±10.0) vs.(122±6.0),(194±8.0)vs.(117±6.5),(202±12.0)vs.(124±8.0),(218±15.0) vs.(126.±8.5),and(224±20.0)vs.(129.±9.0) mm Hg,all P< 0.05].12 weeks after the surgery,the level of serum MMP-9 in the RHR group was kept significantly higher than that in the sham-operated group [(783.4±109.79)vs.(573.4±109.59) ng/mL,P<0.05],and the serum TIMP-1 level was lower in the RHR group than that in the sham-operated group[(313.02±83.9) vs.(976.19±191.1) pg/mL,P<0.05].MMP-9 expressions were significantly higher in the brain and aorta in the RHR group than that in the sham-operated group(both P<0.05),and TIMP-1 expressions were lower than that in the sham-operated group(both P<0.05).The Pearson correlation analysis showed that MMP-9 levels in serum,brain and aorta were positively correlated with systolic blood pressure(r=0.557,r=0.774 and r=0.661,all P<0.05),and TIMP-1 levels were negatively correlated with systolic blood pressure(r=-0.481,r=-0.535 and r=-0.685,all P<0.01).Conclusions Hypertension induces increased MMP-9 and decreased TIMP-1 in serum,brain and aorta in renovascular hypertensive rats.There are consistent alterations of circulating and tissue MMP-9 and TIMP-1 levels in renovascular hypertensive rats.There is a relationship between increased blood pressure and high MMP-9 and low TIMP-1 in serum and tissues.

Objective To investigate the relationship between apolipoprotein B (ApoB),apolipoprotein A Ⅰ (ApoA Ⅰ) and their ratios and intracranial cerebral atherosclerotic stenosis (ICAS) in patients with acute ischemic stroke.Methods The patients with large artery atherosclerotic stroke were enrolled retrospectively.The patients were divided into either an ICAS group or a non-ICAS group based on their vascular imaging data.The blood pressure,blood lipids,blood glucose,ApoB,ApoA Ⅰ,and ApoB/ApoA Ⅰ ratios and demographic data were collected.The differences of the above indicators were compared between the two groups.Results A total of 360 patients with large artery atherosclerotic stroke were enrolled.There were 177 patients in the ICAS group (49.2％) and 183 in the non-ICAS group (50.8％).There were significant differences in the constituent ratios of the patients with hypertension,diabetes and coronary heart disease,as well as the levels of low-density lipoprotein cholesterol,ApoB and ApoA Ⅰ and ApoB/ApoA Ⅰ ratios between the 2 groups (all P ＜0.05).Multivariable logistic regression analysis showed that hypertension (odds ratio [OR] 1.75,95％ confidence interval [CI] 1.04-2.93; P =0.035),diabetes mellitus (OR 2.09,95％ CI 1.31-3.32; P =0.002),coronary heart disease (OR 2.68,95％ CI 1.09-6.57; P =0.031),ApoB ≥ 0.84 g/L (0.84-1.00 g/L:OR 2.68,95％ CI 1.30-5.56; 1.00-1.16 g/L:OR 3.95,95％ CI 1.87-8.40; ＞ 1.00 g/L:OR 6.41,95％ CI 2.82-14.49) and ApoB/ApoA Ⅰ ratio ≥0.60 (0.60-0.73:OR 1.92,95％ CI 1.14-3.24; 0.74-0.91:OR 1.79,95％ CI 1.06-3.02; ＞0.91:OR 3.30,95％ CI 1.92-5.67) were the independent risk factors for ICAS,while ApoA Ⅰ ＞ 1.28 g/L was an independent protective factor for ICAS (OR 0.39,95％ CI 0.16-0.98; P=0.044).Conclusions The increased ApoB level and ApoB/ApoA Ⅰ ratio are the independent risk factors for ICAS,and the increased ApoA Ⅰ level is an independent protective factor for ICAS in patients with acute ischemic stroke.The ApoB/ApoA Ⅰ ratio can be used as a biomarker of ICAS in patients with ischemic stroke in Chinese population.

Objective To investigate the association of cholecystectomy with non-alcoholic fatty liver.Methods The 1 10 adults treated by cholecystectomy,who underwent physical examination in the Third Affiliated Hospital of Southern Medical University from January 201 1 to December 2013,were included in the study.None of the subjects had a history of viral hepatitis and alcohol abuse.All subjects under-went CT scanning and measurement of serum triglyceride and cholesterol levels to confirm the diagnosis of non-alcoholic fatty liver.Results Among the 110 subjects,42 (38.18%)had non-alcoholic fatty liver,including 28 males (45.90%)and 14 females (28.59%);78 (70.91%)had increased blood lipids,and 32 (29.09%)had normal blood lipids.Conclusion Cholecystectomy may be a risk factor for NAFLD.

The purifying operating room with tidiness, antisepsis and easy operation is widely used in more hospitals. The same time, depurative flow, manufacturing skill and technical standards of it are still developing. But filter maintenance and replacing as key component has major difference in different region and season. Through four-year exploration and practice, the methods of filter maintenance and replacing were explained, which carry out the questions of consumption and energy efficiency.

Oil of Eveningprimrose was extracted from the seed of Oenothera biennis by supercritical CO2 extraction.A yield of 20% oil was obtained at 50℃ and 25MPa. Its ?-linolenic acid content was 10. 6%. Acid value and saponification number of the oil was determined.

Objective: To explore the coping strategies of students of military medical university, and the relationship between coping strategies and mental health. Methods: 4786 students of military medical university were assessed with the Simplified Coping Style and SCL-90. Results: 30～40% of the student chose active strategies when facing stresses. Fewer students adopt negative strategies (2～38%). The SCL-90 score of the students of military medical university were lower than students of other military schools. Positive coping strategies were associated with less SCL-90 score (fewer somatic and mental discomfort).Conclusion:Coping strategies are associated with mental health in student of military medical university.

Objective To investigate the effects of Atorvastatin Combined with valsartan on the degree of coronary artery lesion and the level of serum lipoprotein and C reactive protein in patients with coronary heart disea.Methods 95 cases of patients with coronary heart disease from September 2015 to September 2016 in our hospital were randomly divided into observation group and control group,the control group were treated with atorvastatin,observation group of patients in the control group patients on the basis of the combination of valsartan treatment,severity of coronary artery disease,serum lipid and protein levels in patients with C reaction protein levels before and after treatment were compared between two groups.Results After treatment,the patients in the observation group were fibrous plaque,calcified plaque,lipid plaque,mixed plaque were significantly decreased,and lower than that of control group,and control group before and after treatment of fibrous plaque,calcified plaque had no obvious change after treatment,the observation group were HDL,LDL,TG,TC were(2.12±1.01),3.27±0.94),(1.53±0.98),(3.35±1.78)was significantly higher than the control group,the difference was significant,2 months after treatment,3 months to observe the levels in patients with C reactive protein were(10.27±1.78)and(7.26±2.63)was significantly lower than the control group with significant difference,with statistical significance(P<0.05).Conclusion Atorvastatin Combined with valsartan can help to reduce the coronary plaque,regulate lipid metabolism,reduce the level of C reactive protein.

0 05). The detective rate of (85 7%) CgA in the P53 protein positive group(21 cases) was significantly higher than that of 54 5% the P53 protein negative group(55 cases). Conclusions The positive rate of CgA was increased with corresponding to the lower down of differentiation grades . Very close relationship is found between the expression of P53 protein and the incidences of CgA positive cell. It suggested that the expression of P53 protein might play an important role in neuroendocrine differentiation in ovarian mucinous tumors.

[Summary] Between February 2010 and November 2014, a retractor fabricated by stainless steel tongue depressor was used in 30 cases of small incision appendectomy in our department .All the operations were performed via an incision 1.5-3 cm in length.No complications were found .Such self designed retractor can be clinically utilized with characteristics of cheapness , safety, and good exposure of operative field .

Objective:To explore the application effect of small private online course (SPOC) teaching mode in clinical practice teaching of thoracic surgery.Methods:A total of 56 undergraduate students who were internships from May to July 2018 were selected as subjects. They were randomly divided into experimental group and control group. SPOC teaching mode and traditional teaching mode were respectively adopted in the practice teaching, and the teaching effect was evaluated by questionnaires and written examinations. SPSS 22.0 was used for statistical analysis, ttest for independent-sample test, and chi-square test for counting data. There were significant differences when P<0.05. Results:The questionnaire showed that the six teaching effect evaluation indicators of the experimental group were higher than those of the control group ( P<0.01). In terms of written test assessment, the average score of the experimental group was significantly higher than that of the control group, with significant differences ( P<0.05). Conclusion:The SPOC teaching mode can improve the teaching effect of clinical practice in thoracic surgery and improve students' self-learning ability.