Objective:Tumor suppressor gene nitrogen permease regulator like 2 (NPRL2), also called tumor suppressor candidate gene 4 (TUSC4), widely exists in all kinds of human tissues and is highly conserved in different kinds of biological species. The expression of NPRL2 is markedly decreased in many human tumor tissues, such as lung cancer, breast cancer,renal carcinoma, et al. NPRL2 is located in human chromosome 3p2.13, and participates in DNA mismatch modification, regulation of signal transduction du-ring cell cycle progression, and apoptosis induction. NPRL2 gene inactivation is related with the initiation and progression of several malignant tumors. The mechanism underlying the tumor-inhibiting activity of NPRL2 gene is not clear and needs to be further investigated.

BACKGROUND:Along with the medicine development.the prevention and treatment of the atrophy of denervated skeletal muscle have made a remarkable progress,but the clinical curative effect is still not satisfied OBJECTIVE:To summarize the current status of the prevention and treatment methods of denervated skeletal muscle atrophy,and to find a more effective method for the prevention and treatment of the denervated of skeletal muscle atrophy METHODS:Using"denervation,muscle atrophy,treatment"in English for the search term,Medline database from January 1998 to January 2008 was retrieved.Using"denervation,muscle atrophy,treatment:in Chinese for the search term,the Chinese Journal Full-text Database from January 1998 to January 2008,Wanfang Database from January 1998 to January 2008,and Journal of Clinical Rehabilitation and Tissue Engineering Research from January 1 998 to January 2008 were searched.Literature was limited to English and Chinese languages.The muscular endurance and contractile force,muscle wet weight of denervated nerve,and skeletal muscle repair served as the evaluation index The studies on the micro-surgical procedures,physical therapy,biological and chemical therapies,gene therapy for the atrophy of denervated skeletal muscle were included.Except the above methods,other therapies to the denervated skeletal muscle atrophy were excluded.RESULTS AND CONCLUSION:After peripheral nerve damage.the skeletal muscle is denervated and then inevitably atrophy occurs Therefore,the exploration of prevention and treatment of denervated skeletal muscle atrophy has attracted the interests from many domestic and foreign scholars.will certainly become the important task and the research hot spot in the 21st century in the peripheral nerve domain The microsurgery surgery,physiotherapy,biology and chemotherapeutics,gene therapy are the main effective prevention methods for the atrophy of denervated skeletal muscle.At present,this domain.s research already presents the multiple perspectives and various tendencies.The prevention and treatment of denervated skeletal muscle atrophy has already given the pointed management,but in the improvement microcycle,but much works need to carry on in fields of improving microcirculation,preventing cell apoptosis,suppressing the collagen excessive growth,as well as adjusting myogenic regulatory factors expression using gene therapy method in the gene level,but also had the.Along with the tissue engineering,cell culture science,molecular biology,genetic engineering and so on,an unceasing development will be obtained regarding the prevention and treatment of denervated skeletal muscle atrophy.

Atomic layer deposition has been employed for postsynthesis treatment of octadecyl bonded silica (ODS) as a typical reversed-phase stationary phase for high performance liquid chromatography (HPLC) ,in an effort to alleviate peak tailing for basic compounds. With hexamethyldisilazane( HMDS) as an endcapping agent,the ODS packing materials were heated to 250℃,and maintained at that temperature for 6 h,affording packing materials which are highly inert to basic compounds. Chromatographic performance in terms of retention factor and tailing factor of the resulting phases was evaluated using phenol/pyridine and naphthalene/ami-triptyline pairs as probes. The results were compared with those for the same packing treated by liquid phase method and for the commercial products including Zorbax SB-C_(18) and Kromasil C_(18). The separation characteristics of the ODS phase obtained by atomic layer deposition appear to be superior to that by liquid phase method and,comparable with or even better than the commercial products studied. Being solvent-free process amenable to mass production,the described method provides an economical and eco-friendly approach to manufacturing HPLC packing materials on industrial scale.

Objective:To sutdy the effects of the polysaccharide(S3) separated out in 60% alcohol aqueous from OCA on their immunomodulatory functions in mice.Methods:After screening the most effective component of S3 by phagocytic function of macrophage,further tests about the effects both on the humoral immunity and the cellular immunity of S3 in mice were studied.Results:S3 simulated the production of the antibody on the haemolysin test,selectively enhanced ConA induced murine proliferation of lymphocytes(P

This article described the application of the phantom system in the experiment teaching of Endodontics.Through the process of teaching,we realized that the way of simulation in the experiment teaching not only can enhance the enthusiasm of students,but also can consolidate the basis of theoretical knowledge and improve the clinical skills of students.The system has become the necessary equipment and means in experimental teaching of modern stomatology.It is worth further promotion and application in dental teaching.

Objective:To study the chemical constituents in methanol parts of Toddalia asiatica ( Linn) Lam. Methods: The compounds were isolated by silica gel, Sephadex LH-20 column chromatograph and TLC. The structures were elucidated by physico-chemical properties and spectral analysis. Results:Nine compounds were obtained and elucidated as dictamnine(1), hesperetin(2), 4-hydroxy-N-methylproline(3), 8-hydroxy-dihydrochelerythrine(4), hesperetin7-O-β-D-glucopyranoside(5), daucosterol(6), hes-peridin(7), diosmin(8) and neohesperidin(9). Conclusion:Compound 5, 6, 8 and 9 are isolated from the plant for the first time.

AIM: The influence of the seawater on the lipid peroxidation of the muscle tissue wounded by firearm was studied. METHODS: Both two limbs of rabbits were wounded by the steel ball of 250 mg. The wounded rabbits were divided into two group: One is seawater immersed group (SIG), which was dipped in artificial seawater for 30 min. Another is the single wounded group (SWG), which wasn't dipped in seawater. Then, muscle tissues 0.5 cm (A area), 1.5 cm (B area) and 2.5 cm (C area) from wound edge were cut for biochemical analysis at 3, 6, 12, 24 h of post-wound, respectively. The contents of ATP, MDA and SOD activity of all these tissues were detected, pre-wounded muscle tissues were used as control group. RESULTS: The content of MDA in SIG increased obviously at 3, 6 h, respectively, but it decreased slightly at 12 h and increased again at 24 h. The change in MDA in SWG is similar to that in SIG but more slightly. The changes in both the activity of SOD and the content of ATP in two groups is similar to that of MDA. CONCLUSION: Seawater induced more serious lipid peroxidation in the muscle tissue wounded by firearm. [

ObjectiveTo study the effect of lentivirus-mediated microRNA-21 RNAi on biological behaviors in human hepatic cancer cells.MethodsMicroRNA-21 specific siRNA gene was synthesized and cloned into the recombinant lentiviral vector,pGCSIL-GFP.HepG2 cells were infected by microRNA-21 siRNA recombinant lentivirus (miR-21-siRNA-Ⅳ).The HepG2 cells were devided into SI group,NC group and N group in vitro.The expression of the targets of miR-21 was detected by RT-PCR.Cell growth was analyzed by MTT assay.The invasion was dectected by tmnswell method.Apoptosis was detected by Hoechst33258.BALB/c nude mice were randomly divided into SI group and NC group.The growth of transplant tumors in BALB/c nude mice were observed.Results ( 1 ) The expression level of miR-21 was inhibited significantly by miR-21-siRNA-lⅣ.(2) The proliferation of HepG2 was also markedly suppressed in MTT at the 96 h point ( P =0.0031,P ＜ 0.05 ).(3) The number of cells that migrated through the chamber of SI group decreased ( P =0.0004,P ＜ 0.05 ).(4) The cell apopotosis in SI group increased markedly.In addition,the caspase 3 mRNA significantly increased ( P =0.0002,P ＜ 0.05 ).( 5 ) Tumor growth curve was not statistically different between groups ( P =0.0002,P ＜ 0.05 ).ConclusionsMicroRNA-21 specific siRNA suppresses the proliferation and migration of HepG2 cells and induces tumor cell apoptosis inhibiting the growth of transplanted tumor in Balb/c nude mice.

Objective To study the clinic pathologic characteristics and prognosis of rectal cancer between Uyghur patients and Han patients .Methods Data of 196 cases of rectal cancer (58 Uyghur and 138 Han) in the first affiliated hospital of Xinjiang medi‐cal university from January 2008 to February 2010 was collected and analyzed .Then analyzed the differences in their clinical patho‐logical features and prognosis .Results There were significant differences in sex ,age ,blood type ,occupation ,treatment time ,the first symptom ,histological type and DUKES between two nationals (P<0 .05);while there was no significant difference in educa‐tion ,concomitant diseases ,CEA ,tumor location ,tumor size ,gross type ,degree of differentiation and postoperative chemotherapy be‐tween two nationals(P>0 .05);the 5‐year overall survival rate of the patients from Uyghur was shorter than that of the patients from Han (P<0 .05);according to the multivariate analysis ,ethnic served as an independent prognostic factor in the patients who underwent radical resection of their rectal carcinoma (P<0 .05) .Conclusion The Uyghur rectal cancer patients are associated with significantly younger age ,later treatment ,later stages ,more aggressive histopathologic characteristics and significantly worse prog‐nosis than the Han patients .

Objective To discuss the therapeutic effect of ulinastatin combined with dexamethasone on blast-induced acute lung injury (ALI) in rabbits.Methods Thirty-eight New Zealand white rabbits with blast-induced ALI were divided into four groups according to the random number table:injury group (n =8),treatment group Ⅰ (dexamethasone alone,n =10),treatment group Ⅱ (ulinastatin alone,n =10),combination therapy group (dexamethasone + ulinastatin,n =10).In addition,8 uninjured rabbits were randomly selected as control group.Treatment groups were given relevant medication by intramuscular injection at 0.5,12,24,36 and 48 hours after successful modeling.Injury group and control group were given equal volume of isotonic saline at the same time.Parameters of respiratory rate,oxygenation index (PaO2/FiO2),TNF-α,IL-6,IL-8 and neutrophil elastase (NE)were detected before and afterfirst treatment at 6,24,48 and 72 hours.All rabbits were sacrificed after 72 hoars to detect TNF-α,IL-6,IL-8 and NE contents in bronchoalveolar lavage fluid (BALF) and to measure wet to dry lung weight ratio.HE staining was performed to observe pathological changes of lung tissues Results In contrast,respiratory rate was high in injury group at each time point,which increased to the peak (75.0 ± 7.4) times/min at 6 hours far higher than (33.0 ± 4.5) times/min in control group (P ＜0.01).Wet to dry lung weight ratio and TNF-α,IL-6 as well as IL-8 contents of the peripheral blood and BAI.F in injury group were significantly higher than those in control group (P ＜0.01).PaO2/ FiO2 in injury group was decreased to the lowest level of (180.5 ± 12.6)mmHg at 72 hours far lower than (403.7-8.0)mmHg in control group (P ＜0.01).While improvements were observed in treatment groups with respect to these indicators,and significantly much better results were detected in combination therapy group rather than in treatment groups Ⅰ and Ⅱ (P ＜ 0.01).Conclusion Ulinastatin combined with dexamethasone is an effective therapy on blast-induced ALI in rabbits,and the effect is significantly better than that with single use of uliinastatin or dexamethasone.