The characteristics of non-arteritic anterior ischemic optic neuropathy (NAION) include sudden decline of visual acuity, sector-shaped visual field defect connected with the physiological blind spot or hemi-visual field defect, and optic disc edema. Early angiographic abnormalities of optic disc and peripapillary choroidal circulation are important cues for the diagnosis. Angiography combined with visual field test can make the diagnosis more accurate. The pathological mechanism and process of NAION and ischemic cardiovascular/cerebrovascular disease are essentially the same. There are several critical issues in this field we need focus on, including strengthening the cooperation with relevant professional disciplines; improving the ocular ischemia through drugs, intervention and other means; preventing more serious cardiovascular events and intervening in a number of high-risk populations to reduce the incidence of AION.

Objective To investigate the clinical efficacy of Zhuang medical sinew therapy forcervical spondylotic radiculopathy and provide reliable experimental data and scientific evidence for the establishment of clinical technical operation standardization.Method Eighty patients with cervical spondylotic radiculopathy were randomly allocated to treatment and control groups, 40 cases each.The control group took Jingfukang granules with hot water and the treatment group received Zhuang medical sinew therapy.The therapeutic effects were compared after one course of treatment.The 20-point scale score and the VAS score were recorded in the patients before and after treatment.Result Thirty-eight patients in the treatment group and 36 patients in the control group completed the required treatments. The total efficacy rate was 92.1% in the treatment group and 72.2% in the control group;there was a statistically significant difference between the twogroups(P<0.05).There were statistically significant post-treatment differences in the Japanese 20-point scale score and the VAS score between the two groups (P<0.05).Theresults showed that the Japanese 20-point scale score and the VAS score improved more in thetreatment group.Conclusion Zhuang medical sinewtherapy has a marked effect oncervical spondylotic radiculopathy.

Automatic Data Processing ; Computer-Aided Design ; Cone-Beam Computed Tomography ; Expert Systems ; Humans ; Imaging, Three-Dimensional ; Orthodontics, Corrective ; Robotics ; Surgery, Computer-Assisted

AIM:To analyse the risk factors of age, sex, course, best corrected visual acuity(BCVA),diopter and fundus features of high myopes with progressive high myopia. METHODS: A total of 167 patients with high myopes were categorized into four groups: group 1,age of 29 years or younger; group 2,between the age of 30 to 49 years; group 3,between the age of 50 to 69 years and group 4,age of 70 years or older. The refractive errors of all patients were measured without cycloplegia with an autorefractometer. Data of the spherical equivalent(SE) of the refractive errors in diopters (D)and fundus examed by direct ophthalmoscope were used in statistical analyses.RESULTS: The number of female was statistically larger than that of male(P＜0.01),also the disease course was correlated to the age. The visual acuity of high myopes significantly decreased as they grew older including the higher incidence of lacquer cracker, submacular hemorrhage, Fuchs spots, chorioretinal atrophy . CONCLUSION: Female maybe a risk factor of high myopia, advanced age is an important factor of visual acuity decrease. High myopes ought to be treated early to delay the progress of myopia and development of macular degeneration.

Objective To investigate the application of laparoscope,duodenoscope and choledochoscope in the treatment of intra-and extrahepatic bile duct stone.Methods The clinical data of 3 16 patients with intraand extrahepatic bile duct stone who were admitted to the Bethune First Hospital from April 2007 to August 2010were retrospectively analyzed.There were 269 patients with cholecystolithiasis and choledocholithiasis,10 patients with cholesystolithiasis,choledocholithiasis and hepatolithiagis,and 37 patients with choledocholithiasis.Laparoscopic cholecystectomy(LC)+laparoscopic common bile duct exploration(LCBDE)+choledochoscopy was applied to patients with hepatolithiasis or with the diameter of common bile duct≥10 mm;endoscopic sphincterotomy (EST)+LC or LC+EST was applied to patients with the diameter of common bile duct between 10 mm and 5 mm and the diameter of cystic duct<5 mm;LC+laparoscopic transcystic common bile duct exploration(TC-CBDE)+choledochoscopy wag applied to patients with the diameter of common bile duct≤5 mm and the diameter of cystic duct≥5 mm.Results The success rate of operation was 96.8%(306/316).A total of 163 patients received LC +LCBDE+T-tube drainage+choledochoscopy,and the mean operation time,expense,duration of hospital stay were 93.6 minutes,2.8×104 yuan and 9.8 days,respectively,and 5 patients had complications postoperatively.Fifty-four patients received EST+LC,and the mean operation time,expense,duration of hospital stay were 45.0minutes,6.6 days,2.3×104yuan,respectively,and 1 patient had complication postoperatively.Sixty-seven patients received LC+EST,and the mean operation time,expense and duration of hospital stay were 40.0minutes,6.1 days,2.4×104 yuan,respectively,and 2 patients had complication postoperatively.Thirty-two patients received one-stage repair of common bile duct and LC+TC-CBDE+choledochoscopy,and the mean operation time,expense and duration of hospital stay were 97.3 minutes,7.3 days and 2.5×104yuan,respectively,and 1 patient had complication postoperatively.A total of 272 patients were followed up for 12 months,except for 6 patients with recurrence of common bile duct stone,no residual stone or biliary stricture was etected.Conclusion Combined application of laparoscope,duodenoscope and choledochoscope has advantages of less trauma,quick ecovery and fewer complications in the treatment of intra-and extrahepatic bile duct stone.

Objective To explore and establish a kind of dissertation evaluation system which can fully reflects the characteristics of academic medical doctoral training.Methods 41 medical doctoral dissertation evaluation systems and related literatures were collected,to get the current situation of this dissertation evaluation system in our country and find the key points of various evaluation systems.A questionnaire survey was conducted among 350 doctoral supervisors coming from 6 universities so as to define the framework and connotation of evaluation indicators.Using the Delphi expert consultation method,the connotation of evaluation indicators and composition as well as the weight of various indicators was improved.Results The doctoral supervisors who participated in the research considered that building the dissertation evaluation system should follow the principle of science,innovation and fairness,and the evaluation frame-work should have secondary index and evaluation elements.The evaluation contents concluded choosing topic,literature review,basic theory and professional knowledge,paper writing,innovation,research ability and academic morals and so on.And on the basis of this,the paper explored and developed a more scientific and comparative dissertation quality evaluation system with medical specialty.Conclusion The cultivation units should take the educational goal of evaluation target and the requirements of awarding degree into consideration,seeking opinions fully to the first-line supervisors and combing quantitative and qualitative evaluation effectively with scientific and operable principles when specifying evaluation system.

Cyclic amp receptor protein (CRP) is a global transcriptional factor in many prokaryotes, capable of governing nearly half of the total genes in Escherichia coli. Through the method of error-prone PCR or DNA shuffling, we can first obtain CRP mutant library and then get the expected cell phenotype with enhanced resistance. In this article, we reviewed the following desired phenotype: enhanced tolerance towards oxidative stress, improved osmotolerance, enhanced organic solvent (toluene) tolerance, improved acetate tolerance of E. coli fermentation and improved ethanol tolerance during bio-ethanol production. We then concluded that CRP can also be applied in other host cells to get desired phenotypes. Last, we predicted potential applications of mutant CRP transcriptional factor.
Cyclic AMP Receptor Protein ; biosynthesis ; DNA Shuffling ; Escherichia coli ; metabolism ; Fermentation ; Metabolic Engineering

Objective To retrospectively analyze the correlation between uterine fibroid and ovarian blood supply, and to discuss the visualization of ovarian artery and uterine ovarian branch in uterine arterial embolization. Methods A total of 363 patients with clinically-confirmed uterine fibroid were enrolled in this study. Uterine arterial embolization was carried out in all patients. The visualization of ovarian artery and uterine ovarian branch during angiography was recorded. After the procedure the changes of uterine fibroid and ovarian functions were followed up. The results were statistically analyzed. Results The blood supply of uterine fibroid could be divided into three types: unilateral artery as the main source of blood supply (34.16%), bilateral balanced blood supply (43.80%) and unilateral uterine artery (22.04%). The visualization rate of uterine ovarian branch in bilateral balanced blood supply type was significantly higher than that of other two types (P<0.05). The visualization rate of ovarian artery at the side which was lack of blood vessels was higher than that at the opposite side (P< 0.05). Transient ovary functional impairment was observed in patients with visualization of uterine ovarian branch, which restored to normal in three months. No significant difference in the reduction of the tumor mass existed between each other among the different blood supply types. Conclusion The blood supply types of uterine fibroids have a parallel relationship with the ovarian blood supply from ovarian artery and/or uterine ovarian branch. Special attention should be paid to the uterine arterial embolization of the side that is lack of blood vessels, and it is needed to carefully observe the embolization extent, the flow velocity, etc. during the performance of embolization so as to avoid ovarian function impairment.

Objective To observe the cilioretinal artery and its relationship with central visual loss in central retinal artery occlusion(CRAO) patients.Methods A total of 140 CRAO patients (140 eyes) were enrolled in this study.The patients included 83 males and 57 females.The age was ranged from 42 to 75 years old,with an average of (55.70 ± 22.20) years.All the patients were affected unilaterally,including 79 right eyes and 61 left eyes.The disease duration was from 1 to 10 days,with a mean of (4.7±3.9)hours.Central vision and fluorescence fundus angiography were measured for all patients.The central visual loss was divided into 3 types:mild (≥0.1),moderate (finger counting to 0.08) and severe (no light perception to hand movement).The number,length and location of cilioretinal artery were observed.The correlation between cilioretinal artery and central visual loss was analyzed.Results There were 41 eyes (29.3％) with cilioretinal artery,which including 13 eyes (31.7％) with ≥3 cilioretinal arteries,23 eyes (56.1％) with 2 cilioretinal arteries,5 eyes (12.2％) with 1 cilioretinal arteries.The cilioretinal artery was within 1 disk diameter (DD) in length and not reached the macular area in 37 eyes (90.2％),was more than 1DD in length and reached the macular foveal area in 4 eyes (9.8％).The cilioretinal artery located in the temporal side of optic disk in 29 eyes (70.7％),and in other quadrant in 12 eyes (29.3％).The distribution of central visual loss degree as follow:mild in 15 eyes (10.7％),moderate in 50 eyes (35.7％),severe in 75 eyes (53.6％).The difference of central visual loss in the eyes with or without cilioretinal arteries was not significant (x2=0.16,P＞0.05).Conclusions Cilioretinal artery exists in 29.3％ CRAO eyes.There was no close correlation between cilioretinal artery and central visual loss.

Objective To observe the proportion changes of CD4+CD25+FOXP3+ T cells in peripheral blood of patients with Vogt-Koyanagi-Harada disease(VKH)before and after one month of treatment. Methods The peripheral blood samples from 15 patients with VKH disease before and after one month of treatment by glucocorticoid,and from 15 healthy volunteers were collected,and lymphoeytes were separated from them.CD4+CD25+regulatory T cells were Iabeled by antibodies of cell surface marker CD4、CD25 and transcription factor FOXP3.The proportion of CD4+CD25+FOXP3+ T cells were detected by flow cytometry. Results Before the treatment,the percentage of CD4+ CD25+FOXP3+ T cells in periphery blood was(0.30±0.19)%of CD4+ cell in VKH patients,and(1.41±0.52)%in control group,the difference was statistically significant(t=7.665,P＜0.01);after one month of treatment,the VKH patients group was(1.28±0.54)%which close to the control group.However there were two patients whose CD4+ CD25+ T cells inereased extraordinarily after one month of treatment. Conclusions The proportion of CD4+ CD25+ FOCP3+ T cells in periphery blood in VKH patients were lower than control group obviously before treatment,but were close to eontrol group after treatment.Those results indicated that VKH diseases may be associated with the decreased proportion of CD4+ CD25+ regulatory T cells.