A differential diagnosis between primary and metastatic cancers is required in lung cancer patients with a history of breast cancer, However, the limited morphological findings are not enough to differentiate many atypical cases. Essential immunohistochemical and molecular markers are helpful for differential diagnosis. Combined markers currently specific and sensitive to lung or breast carcinomas can improve the efficacy of differential diagnosis between primary lung cancers and metastatic breast cancers.

Peripheral T cells are closely related to nature killer( NK)cells,and share some immunophenotypic and functional properties with NK cells.Peripheral T-cell and NK cell lymphomas are heterogeneous groups of lymphomas,and the subgroup classification is complicated.Currently,the pathogenetic molecular lesions remain not to be deciphered for most entities.However,novel insights into the features of angioimmunoblastic T-cell lymphoma displaying correlation with normal helper T cells,the gene of anaplastic large cell lymphoma,and potential therapeutic biomarkers,have been gained from molecular technology application.

Actins ; metabolism ; Adult ; Glomus Tumor ; metabolism ; pathology ; surgery ; Humans ; Male ; Trachea ; pathology ; Tracheal Neoplasms ; metabolism ; pathology ; surgery ; Tracheotomy ; Vimentin ; metabolism

Acupuncture Therapy ; Defecation ; Female ; Humans ; Ileus ; physiopathology ; therapy ; Middle Aged

Objective To investigate the effects of arginine combined with leptin in treatment of rats with a-cute pancreatitis. Methods Sixty Wister rats were randomly divided into sham operation group (group A), acute pancreatitis group (group B),arginine group (group C),leptin group (group D),arginine plus leptin group (group E),with 12 rats in each group. Acute panereatitis model was developed by retrograde injection of 5% sodium tauro-cholate into cholangiopancreatography in rats. After 6 hours, medicine were given by intraperitoneal injection, ab-dominal aortic blood and pancreatic specimens were taken for measurement of tumor necrosis factor-α(TNF-α), ser-um amylase,nuclear factor-κB (NF-κB) activity of pancreatic tissue, and pathological damage score of pancreatic tissue. Results TNF-α,Amylase,NF-κB activity, pathologic scores were(1.206±0.446)μg/L, (1617.2±18.8) U/L,0.174±0.013,0.6±0. 2 in group A;(3.201±0.321) μg/L, (4643.8±303.4) U/L,0.397±0. 025,7.8±1.3 in group B;(1.845±0.404) μg/L,(3370.9±173.2)U/L,0.255±0.026,3.9±1.1 in group C;(1.996±0.374) μg/L, (3693.7±208.0)U/L,0.274±0.062,4.2±1.2 in group D;(1.440±0.287)μg/L, (2501.0±771.4)U/L,0.211±0.027,2.3±1.0 in group E. Compared with group A, the indicators were significantly in-creased in group B (P < 0.05). Compared with group B, these indicators were reduced in group C and D (P <0.05). There was no significant difference between group C and group D. These indicators in group E were signifi-cantly lower than those in group C and D (P < 0.05). NF-κB in pancreatic tissue were positively correlated with other indicators(P <0.01). Conclusions In treatment of acme pancreatitis in rats, arginine combined with leptin has idenditfied effect, and the effect of combination therapy is better than that of separate medicine.

Objective To investigate the characters of morphology change and protein expres-sion in progressed renal cell carcinoma after the treatment of sorafinib. Methods Clinical data of 2 cases with progressed renal cell carcinoma treated with sorafenib were collected. The HE slices were reviewed. Immunochemistry was used to detect the expression of Vimentin, AE1/AE3, CK7, CK8, CK18, CD10, VEGF, VEGFR2, p53 and Ki-67 levels. Results There was no difference in patho-logic type between before and after the therapy of sorafenib. Both of the 2 cases were showed degener-ation in tumor cell in different degree with fibrosis and necrosis. The expression of renal cell carcinoma related antigens (Vimentin, AE1/AE3, CK7, CK8, CK18 and CD10) had no difference before and af-ter the treatment of sorafinib. The expressions of VEGF, VEGFR2, p53 and Ki-67 were increased, and the expression of Bcl-2 was decreased after the therapy of sorafinib. Conclusions There may be some morphologie differences between the metastatic tumor or the recurrent tumor and primary tumor because of the treatment of sorafenib. However the pathologic type is the same before and after the treatment of sorafenib. The main differences are the degeneration of the tumor cell and fibrosis after the treatment of sorafenib. The expression changes of VEGF and VEGFR2 may be related to the sor-afenib application.

Objective To construct and identify the efficacy of a lentiviral vector harboring RNAi sequence targe-ting NSBP1 gene. Methods Three siRNA targeting the NSBP1 mRNA were designed, the pGCSIL-GFP-NSBP1 lentivirus vectors were constructed and confirmed by DNA sequencing. A total of 293T cells were co-transfected with pGCSIL-GFP-NSBP1, pHelper1.0 and pHelper2.0 for the virus stocks produced, the titer of the virus was test-ed. After lentivirus transfecting into DU145 ceils, Western-blot and MTT methods were used to determine the ex-pression and biological activity of NSBP1 gene, the cells were transplanted into nude mice, then inhibitive effect was observed. Results PCR analysis and DNA sequencing demonstrated that the RNAi sequence targeting the hu-man NSBP1 gene was successfully inserted into the lentiviral vector. The titer of the recombinant]entiviral vector was 2 × 10~8TU/mL. NSBP1 protein expression level in transfected cells was significantly decreased and growth rate of cells transfected with lentivirus was decreased by MTT assay, the downregulation of NSBP1 reduced growth rate of transplantated tumor, whereas tumorgenicity was not influenced. Conclusion The construction of the]entiviral vector of NSBP1 has been successfully prepared and NSBP1 plays an important regulatory role in androgen-inde-pendent prostate cancer cell proliferation.

Objective To assess the dose reduction potential of adaptive statistical iterative reconstruction(ASiR)and model-based iterative reconstruction(MBIR)in pelvic CT with a standard male phantom.Methods A Fluke Biomedical RANDO standard male phantom was scanned with discovery CT750 HD using different tube currents.CT images were reconstructed with FBP,50％ASiR and MBIR.The CT value,the image noise and the contrast-to-noise ratio(CNR)for the sacral vertebra relative to muscle were measured.The volume CT dose indexes(CTDIvo1)and dose-length product(DLP)were recorded.Results Compared with FBP,using 50％ASiR and MBIR had significant reduced image noise and greater CNR.The effective minimal tube currents for displaying sacral vertebra were 250 mA(FBP),180 mA(50％ASiR),and 100 mA(MBIR).With the similar image quality using FBP,the dose was reduced by 28.0％ and 59.9％ using 50％ASiR and MBIR,respectively.Conclusions Using advanced iterative algorithms can reduce image noise,improve CNR,and reduce the radiation dose in pelvic CT examination.

Objective To study the apoptosis pathway of human lung adenocarcinoma cell line A549 induced by 1-β-D-arabinofuranosylcytosine (Ara-C) in vitro. Methods A549 cells were incubated with Ara-C for 72hours in vitro. Biological changes of apoptotic cells were studied by TUNEL staining. Morphological changes of the A549 cells treated with Ara-C were observed by transmission electron microscope. The expressions of p53 and p73 were investigated by Western blotting. Results 1.Apoptotic rates of A549 cells exposure to Ara-C studied by TUNEL staining were higher than that of the control (P＜0.01). 2.Apoptosis body was apparently observed by transmission electron microscope. 3.Endogenous p73 but not p53 was induced and activated in dose-dependent manner upon Ara-C treatment by Western blotting.Conclusion Ara-C can effectively induce apoptosis of A549 cells. DNA damage-induced apoptosis of A549 cells treated by Ara-C is independent of functional p53.Up-regulation of p73 may play an important role that enhances the sensitivity of A549 cells to Ara-C and be partly responsible for p53-independent apoptosis.

Objective:To observe the clinical efficacy of the three-step modified shoulder joint mobilization in treating scapulohumeral periarthritis (SP).Methods:Totally 80 subjects were recruited and divided into a treatment group and a control group by using the random number table, 40 in each group. The treatment group was intervened by the three-step modified shoulder joint mobilization; the control group was by oral administration of Celebrex. After successive 4-week treatment, the improvement of shoulder pain and motor function was observed.Results:The total effective rate was 87.5% in the treatment group versus 80.0% in the control group, and the difference was statistically significant (P<0.05).Conclusion:The three-step modified shoulder joint mobilization is easy-to-operate and effective in treating SP.