Objective To explore the effectiveness of individual rehabilitation therapy in patients with nonspecific low back pain.Methods A total of 50 patients with nonspecific low back pain ( NLBP) between January 2013 and January 2015 were enrolled in this study.Individual rehabilitation therapy was performed based on their different pathogenesis and risk factor of low back pain.Visual analogue score (VAS),Oswestry disability index ( ODI) and Japanese orthopaedic association ( JOA) were used for the assessment of effectiveness of individual rehabilitation therapy.Results Compared with pre-rehabilitation, VAS and ODI scores were significantly decreased at two-week after rehabilitation(P<0.05),and were further decreased at six-week after rehabilitation(P<0.01).The mean score of JOA of 50 patients before rehabilitation was (10.83 ±2.94) score,which was decreased to (24.37±7.55 )score at six-week after rehabilitation(P<0.05),with 12 patients were cured,32 were improved obviously,6 were improved moderately.The rate of efficacy was 100%.Conclusion Individual rehabilitation therapy was an effective option for NLBP,with significant decrease of pain.

OBJECTIVETo systematically evaluate prostate health index (PHI) in predicting the results of prostate biopsy in the diagnosis of prostate cancer.

METHODSWe retrieved the literature relevant to the value of PHI in predicting prostate cancer at prostate biopsy published from the inception to February 2014 in the databanks of PubMed (1966 - 2014), CNKI (1982 - 2014), VIP (1989 - 2014), and Cochrane Library (1999 - 2014). According to the inclusion and exclusion criteria and retrieval strategies, we extracted the data, evaluated the quality of the included literature, and performed meta-analysis using the Meta-Disc 1.4 software.

RESULTSTotally, 64 articles were identified, of which 52 were excluded and 12 included with 1 430 cases of prostate cancer and 2 159 normal or BPH controls. There was heterogeneity among the included studies. Based on the random effect model, in predicting the results of prostate biopsy in the diagnosis of prostate cancer, serum PHI exhibited a pooled sensitivity of 55.1% (95% CI: 0.525 - 0.577), a pooled specificity of 71.5% (95% CI: 0.695 - 0.734), a positive likelihood ratio (P-LR) of 2.379 (95% CI: 1.922 - 2.943), a negative likelihood ratio (N-LR) of 0.515 (95% CI: 0.428 - 0.619), a diagnosis odds ratio (DOR) of 5.268 (95% CI: 3.870 - 7.170), a summary receiver operating characteristic curve--area under the curve (SROC AUC) of 0.7578, and a Q * index of 0.6999.

CONCLUSIONSerum PHI plays a role of auxiliary diagnosis in detecting prostate cancer and can be used in the prediction of the results of prostate biopsy.

Aged ; Biopsy, Needle ; Humans ; Male ; Middle Aged ; Prostate ; pathology ; Prostatic Neoplasms ; diagnosis ; pathology

Objective　To find out if an unique blood mode-vasculogenic mimicry (VM) exist in prostate cancer and analyze its relationship with prognosis.　Methods　The age of the patients was 59 to 72 years,with a mean age of (66.7 ± 11.0) years.All had been confirmed as prostate cancer by biopsy.The level of serum PSA was 15.6 to 76.7 μg/L,with a mean level of (34.6 ± 1.7 ) μg/L.A histochemical and immunohistochemical dual staining method for PAS-CD31 and MMP-2 in 96 prostatic adenocarcinomas were conducted to explore if VM exist in prostate cancer,describe its morphology and distribution.The different expression of MMP-2 in VM positive and VM negative tissue were.The clinical data were collected and analyzed to explore the relationship between disease progression and VM.　Results　Among 96 cases,24 cases were found VM positive,the others were negative.The boundary membrane of VM was PAS positive,which was continuous or incontinuous. A positive correlation between VM density and high Gleason score were found.The tPSA were higher in the VM positive cases than that in VM negatives.The VM positive patients had poorer prognosis than that of the negatives.The MMP-2 expression was found correlated with VM positive (rs =0.60,P ＜ 0.01 ).　Conclusions　VM exists in the prostate cancer as an independent structure,and it is more common in the high-grade cancer.The progression-free survival of VM positive patients could be worse than the negative.MMP-2 and VM could be the indicators of poor prognosis.

Objective: To construct the lentiviral plenti6/V5-D-TOPO vector containing TβR Ⅱ Dnglytk and control vector by developing a lentivirus mediated gene transfer program incorporating a herpes simplex virus thymidine kinase(HSVtk) in the dominant negative TGF-β type Ⅱ receptor (TβR Ⅱ Dnglytk) expression vector. Methods: The PCR methods were used to amplify the genes TβR Ⅱ DN and HSV-tk from the respective plasmids. Then the genes were Linked by recombinant PCR technology to construct the fusion gene TβR Ⅱ Dnglytk and control vector TRANSglytk. According to the operation manual (from the Invitrogen company), ToPe cloning technology were used to construct the plasmids of plenti6/VS-D-TOPO(R)-TβRⅡ Dnglytk and plenti6/VS-D-TOPO -TRANSglytk. Both of the constructed plasmids were verified by sequencing.Results: The constructions of the plasmids of plenti6/V5-D-TOPO(R)-TβR Ⅱ Dnglytk and plenti6/V5-D-TOPO(R)-TRANSglytk were completed smoothly. The DNA sequencing results showed that both the plasmids were constructed correcdy and can be used in the production of infectious lentivirus vectors. Conclusion: Using TOPO cloning technology in the construction of the plasmids of plenti6/VS-D-TOPO -TβR Ⅱ Dnglytk and plenti6/VS-D-TOPO(R)-TRANSglytk and recombinant PCR in the fusion genes are feasible. The recombinant PCR combined with ToPe cloning technology can be the simple, highly efficient and rapid way to construct lentiviral vector and construction of plenti6/V5-D-TOPO(R)-TβR Ⅱ Dnglytk, which will lay a foundation for tumor immunotherapy.

Objective To explore the level of hypersensitive C-reactive protein (hs-CRP)in the patients with acute ischemic stroke(AIS)and its clinical significance.Methods The BNⅡhighly sensitive automatical scattering turbidimetric immunity analy-zer was adopted to detect serum hs-CRP level in 64 cases of AIS and contemporaneous 32 individuals with healthy physical examina-tion as the control group.The data of AIS cases were retrospectively analyzed.Results The positive rate of hs-CRP was 53.3% in the AIS group and 9% in the control group with statistically significant difference between them(P <0.05);the overall levels of hs-CRP in the AIS group was in inconformity to the normal distribution,the median was 4.26mg/L,which in the control group was (0.99 ± 0.72 )mg/L,there was statistically significant difference between them (P < 0.05 );the average level of hs-CRP was (5.03±1.51)mg/L in the AIS male group and (2.79 ± 1.09)mg/L in the AIS female group,the difference between them had sta-tostical significance(P <0.05).Conclusion The serum hs-CRP level in the AIS patients is higher than that in the healthy crowd with statistical difference(P <0.05).The age and sex may be the causes influencing serum level of hs-CRP in the AIS patients.The average age in the AIS patients with positive hs-CRP is higher than that with negative hs-CRP.Middle-aged and elderly people with hypertension and diabetes are much more dangerous than healthy people in getting AIS.

Objective:To investigate the changes of expression levels of TLR9 and IL-6 in the periodontal tissue during the experimental tooth movement of the rats, and the effecst of the MT01 on the expression of TLR9,TRAF6 and IL-6 in periodontal tissue,and to clarify its related mechanisms.Methods:Thirty Wistar rats were randomly divided into MT01 intervention group(n=6) and non-MT01 group(n=24).Force of 0.49 N was applied to move the upper first molars mesially. The rats in Non-MT01 intervention group were sacrificed on the days 3,7,14 and 21, and the rats in MT01 intervention group were all sacrificed on the day 7. RT-qPCR was used to detect the expression levels of TLR9,TRAF6 and IL-6 mRNA in maxillary first molar alveolar bone in each group.Results:The expression levels of IL-6 and TLR9 mRNA in loaded side were higher than those in control side(P<0.05), and reached the maximum levels on the day 7(P<0.01);with the interpose of MT01, the expression levels of TLR9 and TRAF6 mRNA were lower than control side(P<0.01).Conclusion: MT01 could down-regulate the expression levels of TLR9 and TRAF6 during orthodontic tooth movement and eventually resists the inflammation during the tooth movement.

Objective Our hypothesis was if we rendered host' s cytotoxic tumor lymphocytes insensitive to TGF-β,these immune cells could be able to overcome the TGF-β mediated immunosuppression and reject the tumor.We aimed to develop a lentivirus mediated gene transfer program incorporating a herpes simplex virus thymidine kinase(HSV-tk)in our dominant negative TGF-β type Ⅱ receptor(TβRⅡDNglytk)expression vector.So we first need to construct the lentiviral pLenti6/V5-D-TOPO vector containing TβRⅡDNglytk and produce the recombinant lentivirus as the transfection vector.Methods PCR were used to amplify the genes TβRⅡDN and HSV-tk from the respective plasmids.Then the genes were linked by recombinant PCR technology to construct the fusion gene TβRⅡDNglytk and control vector TRANSglytk.According to the operation manual from the Invitrogen company,TOPO cloning technology was used to construct the plasmids of pLenti6/V5-D-TOPO(R)-TβRⅡDNglytk and pLenti6/V5-D-TOPO(R)-TRANSglytk.Both of the constructed plasmids were verified by sequencing.ViraPowerTM Lentiviral System and 293 FT cells provided by Invitrogen were used to produce the recombinant lentivirus vector,the tillers of the lentivirus were determined by 293 cells.Results The construction of the plasmids of pLenti6/V5-D-TOPO(R)-TβRⅡDNglytk and pLenti6/V5-D-TOPO(R)-TRANSglytk were completed succefully.The DNA sequencing results showed that both the plasmids were constructed correctly.Using them we successfully produced infectious lentivirus vectors with appropriate tilters.Conclusions Using TOPO cloning technology in the construction of the plasmids of pLenti6/V5-D-TOPO(R)-TβRⅡDNglytk and pLenti6/V5-D-TOPO(R)-TRANSglytk and recombinant PCR in the fusion genes is feasible.Recombinant PCR combine with TOPO cloning technology can be a simple,highly efficient and rapid way to construct lentiviral vector.The production of infectious lentivirus with appropriate tilters using 293FT is suitable and feasible.The construction of pLenti6/V5-D-TOPO(R)-TβRⅡDNglytk and production of the infectious lentivirus will lay a foundation for immunotherapy of prostate cancer.

Objective To investigate the changes of serum cardiac troponin I(cTnI)and brain natriuretic peptide(BNP)in heart failure of children with pneumonia and their relationship with heart function.Methods Thirty healthy children aged from 5 months to 3 years old were randomly selected with 17 male and 13 female(healthy group).Thirty children with severe heart failure aged from 3 months to 2 years old were selected at the same time with 21 male and 9 female(heart failure group).Thirty children with ordinary pneumonia aged from 3 months to 3 years old were also sampled with 16 male and 14 female(ordinary pneumonia group).The peripheral bloods of 2-3 mL of all children were taken.The BNP level were measured by enzyme-linked immunosorbent assay and the cTnI level was determined by micro-particle enzyme immunoluminescent.Left ventricular ejection fraction(LVEF) and left ventricular fractional shor-tening(LVFS)were detected by echocardiography.SPSS 11.0 software was used to analyze the data.Results The levels of cTnI [(0.389?0.030) ?g/L] and BNP [(0.572?0.090) ?g/L] of heart failure group increased significantly compared with healthy and ordinary pneumonia group,while their LVEF and LVFS decreased significantly(Pa

Objective To investigate the correlation of platelet-to-lymphocyte ratio(PLR)with depression in patients with maintenance hemodialysis(MHD)based on propensity score matching(PSM).Methods General information such as age,gender and underlying diseases,biochemical indicators,and Beck Depression Inventory(BDI)scores of patients in hemodialysis centers of 20 Grade Ⅲ and Level A hospitals in Guizhou Province from June to August 2023 were collected.Pa-tients were divided into depression group and non-depression group based on the occurrence of depres-sion,and differences in clinical characteristics were compared between the groups.A 1 to 1 propensi-ty score matching was used to obtain patients with similar baseline data(gender,age,education lev-el)in the depression and non-depression groups,and differences in clinical characteristics between the two groups were compared.Participants were divided into Q1,Q2,Q3 and Q4 groups according to the quartiles of PLR,and differences in clinical characteristics among the groups were compared.Lo-gistic regression analysis was used to evaluate the relationship between PLR and depression in MHD pa-tients.Receiver operating characteristic(ROC)curve was used to assess the value of PLR in predicting depression in MHD patients.Results A total of 2,081 MHD patients were included in this study,including 1,286 males(61.8％),with a mean age of(54.3±14.7)years.A total of 368 pa-tients(17.7％)had depression,and 359 pairs were successfully matched after propensity score matching.The depression group had higher rates of diabetes and PLR,but lower serum creatinine and hemoglobin levels(P＜0.05).The risk of depression in the PLR Q4 group was 22.654 times than that of the Q1 group(95％CI,12.713 to 40.369,P＜0.001).After initially adjusting for age,diabetes,and hypertension,the risk of depression in the PLR Q4 group was 28.272 times than that of the Q1 group(95％CI,15.390 to 51.937,P＜0.001).After further adjusting for serum creatinine,hemoglobin,albumin,white blood cell count,total cholesterol,monocyte-to-lymphocyte ratio,systemic immune-inflammation index,and neutrophil-to-lymphocyte ratio,the risk of depres-sion in the PLR Q4 group was 10.557 times than that of the Q1 group(95％CI,3.461 to 32.201,P＜0.001).The ROC curve analysis results indicated that the sensitivity of PLR in predicting de-pression in MHD patients was 78.0％,the specificity was 73.5％,the optimal threshold was 114.14,and the area under the curve was 0.798(95％CI,0.765 to 0.832,P＜0.001).Conclu-sion PLR is an independent risk factor for depression and has a high predictive value for depres-sion in MHD patients.

Objective To investigate the correlation of platelet-to-lymphocyte ratio(PLR)with depression in patients with maintenance hemodialysis(MHD)based on propensity score matching(PSM).Methods General information such as age,gender and underlying diseases,biochemical indicators,and Beck Depression Inventory(BDI)scores of patients in hemodialysis centers of 20 Grade Ⅲ and Level A hospitals in Guizhou Province from June to August 2023 were collected.Pa-tients were divided into depression group and non-depression group based on the occurrence of depres-sion,and differences in clinical characteristics were compared between the groups.A 1 to 1 propensi-ty score matching was used to obtain patients with similar baseline data(gender,age,education lev-el)in the depression and non-depression groups,and differences in clinical characteristics between the two groups were compared.Participants were divided into Q1,Q2,Q3 and Q4 groups according to the quartiles of PLR,and differences in clinical characteristics among the groups were compared.Lo-gistic regression analysis was used to evaluate the relationship between PLR and depression in MHD pa-tients.Receiver operating characteristic(ROC)curve was used to assess the value of PLR in predicting depression in MHD patients.Results A total of 2,081 MHD patients were included in this study,including 1,286 males(61.8％),with a mean age of(54.3±14.7)years.A total of 368 pa-tients(17.7％)had depression,and 359 pairs were successfully matched after propensity score matching.The depression group had higher rates of diabetes and PLR,but lower serum creatinine and hemoglobin levels(P＜0.05).The risk of depression in the PLR Q4 group was 22.654 times than that of the Q1 group(95％CI,12.713 to 40.369,P＜0.001).After initially adjusting for age,diabetes,and hypertension,the risk of depression in the PLR Q4 group was 28.272 times than that of the Q1 group(95％CI,15.390 to 51.937,P＜0.001).After further adjusting for serum creatinine,hemoglobin,albumin,white blood cell count,total cholesterol,monocyte-to-lymphocyte ratio,systemic immune-inflammation index,and neutrophil-to-lymphocyte ratio,the risk of depres-sion in the PLR Q4 group was 10.557 times than that of the Q1 group(95％CI,3.461 to 32.201,P＜0.001).The ROC curve analysis results indicated that the sensitivity of PLR in predicting de-pression in MHD patients was 78.0％,the specificity was 73.5％,the optimal threshold was 114.14,and the area under the curve was 0.798(95％CI,0.765 to 0.832,P＜0.001).Conclu-sion PLR is an independent risk factor for depression and has a high predictive value for depres-sion in MHD patients.