Objective To study the distribution and pharmacokinetics of curcumin in tumor-bearing mice.Methods Curcumin injection(100 mg/kg)was injected to the tumor-bearing mice through tail vein.The liver,kidney,lung,heart,parenchymal tumor and ascitic fluid were acquired to abstract curcumin at 20,40,100 minutes after injection respectively.A HPLC method was established for determination of curcumin.Results Curcumin contents in the liver,kidney,lung,heart,parenchymal tumor and ascitic fluid were 6.40,0.98,0.13,0.06,0.05 and 0.02 ? g/g after injection of 20 minutes respectively.While after 40 minutes,the contents reduced to 0.20,0.06 and 0.02 ? g/g in the liver,kidney and lung respectively,and could not been detected in other tissues.After 100 minutes,curcumin was only detectable in the liver(0.04 ? g/g).Conclusion Curcumin distribution is focused in the liver of tumor-bearing mice,while the focal concentration is lower .Metabolism of curcumin in vivo is fast.

This study aims to investigate the function of lmo2363 gene in stress resistance of Liste-ria monocytogenes strain LM83-1.In this study,the lmo2363 gene deletion strain and complement-ation strain of Listeria monocytogenes were constructed using overlapping extended PCR and ho-mologous recombination techniques,and the growth ability,stress survival rate and biofilm forma-tion ability of wild,deletion strain and complementation strain were compared under different stress environments.lmo2363 gene deletion strain and complementation strain of Listeria monocy-togenes were successfully constructed in this experiment.The growth curves showed that the growth capacity of the deletion strain was weaker than the wild strain LM83-1 under 4 ℃,7％NaCl,10％NaCl,3.5％ethanol,4.0％ethanol and pH5 stress(P＜0.001).The results of stress survival test showed that the survival rate of the deletion strain was significantly lower than the wild strain after 1 h treatment with pH3 and 10 mmol/L H2 O2 stress(P＜0.010).The biofilm forming ability of the deletion strain was decreased compared with that of the wild strain(P＜0.050).This study confirmed that lmo2363 gene mediated the adaptation of LM to low temperature,high osmotic pressure,ethanol and acid stress environment and affected the formation of LM bio-film.This study laid a foundation for further exploring the function of lmo2363 gene in the stress resistance process of Listeria monocytogenes.