BACKGROUND: The use of human umbilical cord blood(CB) as a source of hematopoietic stem cell transplantation for the treatment of pediatric diseases has been well established. In previous study, we reported the method of improving the recovery rate of cryopreserved CB mononuclear cells after thawing and the possibility of long-term cryopreservation. In this study we compared the recovery rate of the cryopreserved mononuclear cells from large volume of CB according to various freezing conditions and stored CB mononuclear cells as a source of transplantation. METHODS: Twenty five CB from Kyung-pook National University Hospital and the Hana Obstetric/Gynecology Hospital, Taegu, Korea were used. The mononuclear cell separation was done with Hypaque and CD34+ cells were collected by Fenwal with the monoclonal antibody and immunobeads within 6 hours after collection. The cell count and the viability rate was done by hemocytometer with trypan blue exclusion method. The mononuclear cells were cryopreserved with two different concentrations of fetal bovine serum and of dimethylsulfoxide and with two different methods of direct and programmed freezing in the liquid nitrogen. The recovery rate was compared after thawing. The differentiation ability of thawed mononuclear cell was performed with in vitro colony culture using methylcellulose medium and GM-CSF. The family history of inherited blood cell diseases, tests for viral(HIV, HBV, HCV and CMV) and bacterial (syphilis, etc) contamination and HLA and blood typing were done for the future transplantation source. RESULTS: The mean volume of collected CB was 117(49-150)ml and the mean number of mononuclear cells was 5.0(2.8-7.5) x 10(6)/ml. The viability rate was 95.3-98.6%. The recovery rate of mononuclear cells was higher in the group of medium containing 10% of fetal bovine serum and 10% of dimethylsulfoxide after 30 days programmed freezing in liquid nitrogen(-196 degrees C). The recovery rate of long-term(180 days) cryopreserved mononuclear cells was 72.8%. The colony formations in culture of mononuclear cells were not different between before-and 14 days after-freezing. The seven total volume of CB mononuclear cells which had no family history of inherited blood cell diseases, no viral and bacterial contamination were cryopreserved after HLA and blood typing for the future transplantation. CONCLUSION: In summary, because the long-term cryopreservation of CB mononuclear cells was possible with good recovery rate we expect the establishment of CB bank will overcome the limitation of donor in Korea in the near future.
Blood Cells ; Blood Grouping and Crossmatching ; Cell Count ; Cell Separation ; Cryopreservation* ; Cytomegalovirus ; Daegu ; Diatrizoate ; Dimethyl Sulfoxide ; Fetal Blood* ; Freezing ; Granulocyte-Macrophage Colony-Stimulating Factor ; Hematopoietic Stem Cell Transplantation* ; Hematopoietic Stem Cells* ; HIV ; Humans ; Korea ; Methylcellulose ; Nitrogen ; Syphilis ; Tissue Donors ; Trypan Blue ; Umbilical Cord

No abstract available.
Bone Marrow Transplantation* ; Bone Marrow*

No abstract available.
Hemostasis* ; Thrombosis*

Acute cerebellitis is a rare inflammatory syndrome and is one of the important causes of acute cerebellar dysfunction in children. The cerebrospinal fluid (CSF) IgG index is most often tested clinically in the diagnosis of multiple sclerosis. However, it is not specific to multiple sclerosis, and can be elevated in a variety of neurologic diseases. A 7-year-old boy with acute cerebellitis presenting with sudden onset headache and subtle cerebellar dysfunction demonstrated an elevated CSF IgG index (1.1) and an absence of oligoclonal bands. On the seventeenth day, the follow-up CSF IgG index was 0.71. Two-month and one-year follow-up magnetic resonance imaging revealed cerebellar atrophy, although the patient showed no neurologic deficit. To the best of our knowledge, this case report is the first to describe acute cerebellitis accompanied by an elevated CSF IgG index and an absence of CSF oligoclonal IgG bands. These findings suggest that a breach in the blood-brain-barrier might occur in acute cerebellitis.

No abstract available.
Superoxide Dismutase* ; Superoxides*

No abstract available.
Daejeon*

No abstract available.
Female* ; Fetus* ; Humans ; Male*

No abstract available.
Hemophilia A* ; Humans

A case of the neurovascalar island pedicle graft for restoration of sensation after amputation of both thumb fingertip pulp in 17 years old male patient is presented at Department of Orthopedic Surgery, College of Medicine, Korea University. Tho island pedicle graft is suggested by Esser firstly and it is very difficult and excitement. At postoperative follow up check within one month, we obtained very good result without other complications.
Amputation ; Follow-Up Studies ; Humans ; Korea ; Male ; Orthopedics ; Sensation ; Thumb ; Transplants

No abstract available.
Humans ; Infertility* ; Infertility, Male* ; Male ; Male*