Objective:To observe the effect of low intensity pulsed ultrasound on early experimental osteoarthritis of rabbit knees.Methods:Experimental osteoarthritis of rabbit knees were made by Hulth's method.A total of 30 rabbits were divided averagely into 3 groups randomly.One hind knee of 10 rabbits of each group were treated with LIPU randomly while the others as control for 2、4 and 8 weeks respectively.All animals were sacrificed after LIPU.Then the histo-histochemical changes were evaluated by Mankin's score method.Results:Mankin's scores showed that the pathological changes in LIPU group were slighter than those in control group.Conclusion:LIPU can slow down the disease progress of experimental osteoarthritis of rabbit knee.

OBJECTIVE:To establish early warning mechanisms of antibiotics drug resistance,and to provide reference for ra-tional drug use in the clinic. METHODS:The consumption amount of top 3 to 10 drugs in the list of consumption sum and amount in outpatient prescription and inpatient medical orders of our hospital during 2014 was analyzed statistically,including antibiotics, anti-tumor drugs,drugs for cardiovascular system,drugs for digestive system,biologicals. Top 5 Gram-positive bacterial and Gram-negative bacterial which induced infection in our hospital in Nov. 2014 was analyzed statistically in terms of resistant rate. RE-SULTS:Total consumption sum of drugs was 16 038.28 ten thousand yuan in our hospital in 2014,accounting for 27.95%;antibi-otics drugs accounted for 9.55%(1 531.44 ten thousand yuan) of total consumption sum,drugs for cardiovascular system 3.30%(529.60 ten thousand yuan),drugs for digestive system 8.32%(1 333.68 ten thousand yuan),anti-tumor drugs 2.93%(469.27 ten thousand yuan),and biologicals 3.50%(561.22 ten thousand yuan). 1 575 bacterial samples were detected in Nov. 2014,and bacte-rial were detected in 358 samples with positive rate of 22.73%. 136 Gram-positive bacterial were detected,and top 5 were Staphylo-coccus epidermidis, Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium and Streptococcus agalactiae. 222 Gram-negative bacterial were detected,and top 5 were Escherichia coli,Klebsiella pneumoniae,Acinetobacter baumannii,Hae-mophilus influenzae and Enterobacter cloacae. CONCLUSIONS:Five categories of drugs used in our hospital is rational compara-tively,and the proprotion of antibiotics in total consumption sum is much lower than other hospitals;control and standardization of antibiotics use has made some achievements.

Objective]Observe the clinical effects of acupuncture treatment of athletes uterine bleeding. [Method]This study included 90 patients with uterine bleeding, randomly divided into three groups, treatment group 30 cases treated by acupuncture. The western medicine control group 30 cases treated with western medicine. Chinese medicine control group 30 cases were treated by traditional Chinese medicinal broth. One treatment course(after 30d) observation curative effect.[Results]The western medicine control group 15 cases cured, 6 cases were markedly effective, 6 cases better, 3 had no effect, the total effective rate was 90%. Traditional Chinese medicine control group 21 cases cured, 6 cases had marked effect, improvement in 3 cases, the total effective rate was 100%. Treatment group: 24 cases were cured, 3 cases had marked effect, improvement in 3 cases, the total effective rate was 100%.[Conclusions]Three treatments had curative effect, but the side effects of western medicine stopped taking the drug repeatedly; for the special athletes, the acupuncture treatment is worthy of popularization and application.

Objective To investigate the influence of laparoscopic resection of colorectal cancer on (postoperative) micrometastasis of tumor cells.Methods Using cytokeratin,CK-20 mRNA as target gene,the RT-PCR method was used to examine the pre-and post-operative changes in the number of tumor cells in the peripheral blood of 48 cases of colorectal cancer.Results After operation,the number of tumor cells in(peripheral) blood was significantly higher in the laparoscopic surgery group as compared to that of traditional(laparotomy) operation group(P0.05),but was significantly related to positive rate of proliferating cell nuclear antigen(PCNA)(P

Tyrosine kinase receptors mediate many critical cellular functions that contribute to tumor progression and metastasis.Recepteur d'origine nantais(RON)belongs to a subfamily of receptor tyrosine kinases(RTK)with unique expression patterns and biological activities.RON is activated by a serum-derived growth factor macrophage stimulating protein(MSP),primarily expressed in cells of epithelial origin.RON activation induced cell transformation,growth,migration and matrix invasion.We review RON molecular structure,activation,and carcinogenic mechanism related.It will contribute to cancel treatment and to discuss RON expression in the tumor.

Objective To explore the risk factors,composition of pathogen and drug sensitivity of pulmonary infection in neurosurgical intensive care unit(NICU) and provide basis forclinical effective prevention and appropriate treatment.Methods Retrospective analysis the detected pathogenic strains,theirdrug resistance and predisposing factors of the cases of pulmonary infection in NICU from January 2010 to September 2011.Results Among the isolated 602 bacteriastrains,363 strains (60.2％ ) were G-bacteria,71 strains ( 11.8％ ) were G + bacteria,168 strains (28 ％ )were molds.In G-bacteria,the main strains are acinetobacter and pseudomonas aeruginosa,and in G+ bacteria,staphylococcus epidermidis based most.Conclusion Acinetobacter has become the dominant flora of pulmonary infection in NICU and it has multidrug resistance to antibiotics in clinical.The Specific pathogen should be indentified through bacteriological examination of sputum as soon as possible,and the antibiotics should be used alternatively based on their drug sensitivity to control lung infection in critically ill patients.

Objective To understand the the current situation of the slogans in the schistosomiasis health education from the linguistic aspect.Methods Sixty-two slogans in the schistosomiasis health education were collected,then the rationality and validity of them were analyzed from two aspects :the language form and the semantic content.Results Most of the slogans had the appropriate language form and the rational semantic content,they could propagandize the policy and educate the people very well,but there were still some slogans had the inappropriate language form or have the irrational semantic content and so on.Conclusion In order to make the slogans in the schistosomiasis health education more effective,we should attach importance to the language form and the semantic content of them.

Objective:To observe the clinical effect of Desensitizer Oravive combined Er:YAG laser irradiation on dentin hypersen-sitivity.Methods:240 molars in 86 patients with dentin hypersensitivity were divided into 2 groups(n =1 20).In control group De-sensitizer Oravive was applied to the teeth surface for 30 s;in combination group the teeth surfaces were treated by Er:YAG laser irra-diation for 60 s,and then by Desensitizer oravive for 30 s.Each tooth was treated only once.Pain and pain relief were evaluated by VAS before treatment and immediately,4,8 and 1 2 weeks after treatment.Data were statistically analyzed.Results:Before treat-ment,VAS showed no significant difference between the groups(P >0.05).Both groups exhibited lower VAS after treatment(P <0. 05),VAS values were lower(P <0.05)and the effective rate was higher in the combination group than those in the control group at each treatment stage(P <0.05).Conclusion:Desensitizer Oravive combined with Er:YAG laser is more effective than Desensitizer Oravive in the treatment of dentin hypersensitivity.

Objective To analyze the discourse right construction of China’s medical aid to Africa,so as to provide evidenc?es for improving the effect and sustainable development of China’s medical aid to Africa. Methods The documents of the dis?course right that China constructed in the medical aid to Africa were selected at different periods as discourse samples. The achievement and deficiency were analyzed from four aspects,namely confident,charismatic,influential,and dominant. Re?sults China’s medical team made much in the discourse right construction in their aid to Africa,but some China’s medical team members were still too cautious and too low?key. China’s medical team gained the trust of the people of Africa,but the lan?guage communication difficulties still hampered in China’s medical aid to Africa. Chinese medical team were widely praised by the African society,but in the west,some African media,even China’s media still neglected to report China’s doctors. China’s international discourse right were greatly improved,but western countries still dominated the public opinion. Conclusion Chi?na should refer to the actual situation of medical aid to Africa to strengthen the construction of discourse right.

Aim To investigate the toxicity of isopsor-alen in HepG2 cells and its effects on bile acid, bile acid synthesis and transport. Methods Cell viability was evaluated by MTT assay and bile acid was deter-mined inside HepG2 cells, with exposure to various isopsoralen for 24h. The mRNA transcription of BSEP, MRP2, MRP3, NTCP, OATP2, OSTα, CYP7A1, CYP27 A1 , FXR and PXR were assessed by real-time PCR. Results The cell viability was decreased dose-dependently with isopsoralen in HepG2 cells, and IC50 was 118. 1μmol·L-1 exposure to isopsoralen for 24h. Bile acid inside cells significantly increased with 100 and 400 μmol · L-1 isopsoralen. Isopsoralen caused the down-regulation of MRP2 , MRP3 , CYP7 A1 mRNA at 25 μmol · L-1 . Beside these, the up-regulation of OATP2,OSTα,CYP27A1,FXR,PXR with 100 μmol· L-1 isopsoralen, but there was no significant change of BSEP and NTCP. Conclusion The results show that isopsoralen induces bile acid accumulation and cytotox-icity which may be associated with the down-regulation of MRP2, MRP3 in HepG2 cells.