Objective To study the etiology of surgical infection and antibiotic resistance of infective bacteria,so as to provide evidence for clinical treatment.Method The composition and antibiotic resistance in surgical specimens of pathogenic bacteria from January 2008 to December 2010 was analyzed retrospectively.Results A total of 321 pathogens isolated,Gram-negative bacteria 201,accounting for 62.6％,Gram-positive cocci 114,accounting for 35.5％,Fungus 6,accounting for 1.9％.Escherichia coli 116(36.1％),Klebsiella pneumoniae 24(7.5％),Staphylococcus aureus 34(10.6％),Pseudomonas aeruginosa 18(5.6％),Enterococcus faecalis 16(5.0％).Staphylococcus species of Staphylococci methicillinresistant Staphylococcus atureus(MRS),Coagulase-negative staphylococci MRS and ultra wide-spectrum beta-lactamases(ESBLs)detection rate was 52.9％(18/34),83.7％(36/43)and 35.0％(49/140),respectively.The resistance rates of Gram-negative bacilli to piperacillin/tazobactam,ceftazidime,amikacin,imipenem and meropenem were below 20％,Gram-positive cocci,staphylococci to new compound sulfamethoxazole,minocycline,rifampin,vancomycin,teicoplanin and fusidic acid resistance rates were less than 40％,various pathogens had varies resistance.Conclusions Specimens of surgical infection pathogens widely distribute in different drug-resistant bacteria,some strains of multi-drug resistant infection,causing clinical failure of empirical therapy,clinical laboratory susceptibility results should be based on the rational use of antibiotics,to avoid the overuse of antibiotics.

Objective:To investigate the association between anxiety and the change of humoral immune functions and its correlation with HLA-DQB1 polymorphisms.Methods:Total 31 resident doctors were selected randomly and tested by State-Trait Anxiety Inventory(STAI).IgG,IgA,IgM,complement C3 and complement C4 were detected with BECKMAN array360 system;HLA-DQB1*02、*03、*04、*05 and*06 alleles were individually amplified by polymerase chain reaction(PCR)using exon2 group-specific primers.The correlation between immune function and HLA-DQB1 polgmorphisms were investigated.Results:Statistical analysis showed that there was positive correlation with State Anxiety (Ta) and complement C3,either Trait Anxiety (Tc) and complement C3.There was significant difference between HLA-DQB1*02 positive and negative in Ta (P＜0.05),while no difference in complement C3(P＞0.05).There was significant difference between HLA-DQB1*04 positive and negative in Ta and Tc(P＜0.05),while no difference in complement C3(P＞0.05).Conclusion:Anxiety could change some humoral immune functions and this is related with HLA-DQB1 polymorphism.

To establish a new method for the determination of chondroitin sulfate. The method was based on the interaction of sodium chondroitin sulfate with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole to yield a violet product, 6-mercapto-3-substituted-s-triazolo-\[4,3-b\]-s-tetrzaine derivatives. Absorbance at 540 nm obeyed Beer's Law within the concentration range from 0.1 to 1.0 g?L~(-1)and the results were precise and accurate. The method was operationally simple and had a good precision and sensitivity.

Objective To investigate the efficacy of intensity-modulated radiation therapy(IMRT)and the prognosis factors in advanced cervical cancer.Methods A total of 218 cases of cervical cancer patients treated in Affiliated Tumor Hospital,Guangxi Medical University,between July 2007 and July 2012,were divided into IMRT group and routine radiotherapy group (conventional group,94 vs 124 cases).To compare the short-term effects of two groups by Chi-square test,and the incidence rate of 1,3,5-year survival by rank sum test ; to compare the acute and chronic toxicity of the two groups by rank sum test.And to analysis prognostic factors by log-rank test and Cox proportional hazard model and multiple linear regression analysis.Results (1) The effective rate in IMRT group was 95.7％ (90/94),which in the conventional group was 86.3％ (107/124; P ＜0.01).The 1,3,5-year overall survival rates in IMRT group were not significant difference than those in conventional group (87.6％ vs 90.1％,73.4％ vs 81.5％,and 72.3 ％ vs 75.7％ ;all P ＞ 0.05).The rate of acute gastrointestinal toxicity was 41.5 ％ (39/94) in IMRT group,vs 42.7％ (53/124) in conventional group(P ＜ 0.01); and the incidence of acute hematopoietic toxicity in IMRT group was 76.6％ (72/94),which was 78.2％ (97/124) in conventional group (P ＜0.01) ; the incidence of acute genitourinary toxicity was 12.8％ (12/94) in IMRT and 8.1％ (10/124) in conventional group(P =0.248).The late gastrointestinal,genitourinary and hematopoietic toxicities were 18.1％ (17/94),16.0％ (15/94) and 25.5％ (24/94),which were lower than those in conventional group 91.9％ (114/124),47.6％ (59/124) and 56.4％ (70/124),respectively (all P ＜0.01).(2) Univariate analysis showed that the International Federation of Gynecology and Obstetrics (FIGO) stage,histological grade and tumor size were closely associated with 5 years disease-free survival rates and overall survival rates (all P ＜ 0.05).Multivariate analysis showed that FIGO stage and histological grade were closely associated with 5 year overall survival rates (all P ＜ 0.01).FIGO stage and histological grade were independent factors to patients' 5-year overall survival rates in cervical cancer (P ＜0.01).Conclusion IMRT would be to reduce the acute and chronic toxicity,and to improve the quality of patients' life,but did not improve the 5 years survival rate in advanced cervical carcinoma.

Objective To evaluate the laparoscopic hysterectomy surgery continuous infusion of low-dose sufentanil combined with propofol and remifentanil anesthesia postoperative hyperalgesia influence.Methods Patients were collected from June 2013 to June 2015 in our hospital undergoing laparoscopic hysterectomy 60 cases.According to the principles of randomized, divided into sufentanil group (SF group) and 30 cases of normal saline group (NS group) 30 cases.Two intraoperative remifentanil 0.1-0.2 μg/(kg? min) and propofol 4-8 mg/(kg? h) to maintain anesthesia.After the operation began, SF group and NS group were sufentanil and saline.Record calls eyes, recovery of spontaneous breathing, extubation time;after waking 5 min, 30 min, 12 h, 24 h, 48 h pain (visual analogue scales, VAS) score; before induction (T0), after induction (T1), after intubation (T2), surgery (T3), extubation (T4), after extubation 10 min (T5) heart rate (HR) and mean arterial pressure (MAP); excessive sedation, respiratory depression, intraoperative awareness, restless when awake, intraoperative after nausea/vomiting, tramadol and so on.Results SF group and NS group recovery of spontaneous breathing, eye opening and extubation call time was not statistically significant;after SF group wake 5 min, 30 min and 4 h VAS scores lower than the NS group (P <0.05); in T4, T5 point comparison, SF group MAP and HR lower than the NS group (P <0.05); SF group restless when awake, pain (VAS score≥3), postoperative use of tramadol was lower than NS group (P<0.05).Conclusion Intraoperative continuous infusion of low-dose sufentanil improve laparoscopic hysterectomy in patients under anesthesia using propofol combined with remifentanil hyperalgesia, with good prospects for clinical application of anesthesia.

Objective: To analyze the application value of radionuclide myocardial perfusion imaging in the localization and diagnosis for stable angina pectoris in clinical practice. Methods: A total of 70 patients who had been diagnosed with stable angina pectoris (SAP) were enrolled, and all patients underwent ATP-loaded ATP-99Tcm-MIBI (99Tcm-MIBI) myocardial perfusion imaging (MPI) and coronary angiography (CAG) were used to compare the accuracy of MPI imaging in localization and diagnosis for SAP. Results:In the 70 cases of patients with two methods of examination: ①In MPI diagnosis, positive patients were 50 cases, and the positive predictive value of SAP, sensitivity, negative predictive value and specificity were, respectively, 92.0%, 90.2%, 75.0% and 78.9%. There were 45 patients were consistently diagnosed as SAP by the two methods and the total compliance rate was 90.0%. ②in 70 patients, 51 cases were diagnosed as SAP by CAG examination, the positive rate was 72.9%; ③in 50 MPI positive patients, 46 patients were consistent with the results of CAG diagnosis, and the positive rate was 92.0%. The difference between the two methods was statistically significant (x2= 5.72, P<0.05). Conclusion: 99Tcm-MIBI myocardial perfusion imaging can be used as a gatekeeper in patients with stable angina pectoris, and has high application value in the location and diagnosis for stable angina pectoris. The diagnosis and treatment links of stable angina pectoris can save costs and provide a reliable evidencet for clinical practice.

Objective To study CT value in diagnosis and differential diagnosis of submandibular tumor.Methods The CT features of submandibular tumors proved by pathology in 20 cases were analyzed retrospectively and compared with pathologic findings. Double-blind was used to evaluate the value of CT in differentiating both malignant and benign submandibular tumors. Results Of 20 patients,8 were benign tumors, 6 appeared as homogeneous density and well-defined, 2 appeared as inhomogeneous density and partial ill-defined.12 case were malignant of them, 10 were irregular in shape, 8 were heterogeneous indensity, 7 were ill-defined, 5 were well-defined in shape, 4 were homogeneous indensity. Double-blind indicated that 75% submandibular tumors could be qualified by CT, but could not determine the histological type of tumors. Conclusion CT can differentiate most of malignant submandibular tumors from benign tumors, but can not be used to categorize the histological type of tumors.

Objective To explore the efficacy and protective mechanism of calpain, a calcium activated neutral proteinase as a vaccine candidate molecule. Methods Anti calpain sera were prepared by immunized BALB/c mice with recombinant calpain. Anti calpain sera, schistosomula of Schistosoma japonicum , and mouse eosinophils were incubated at 37 ?C , 5% CO 2 for 48 hours, and the adherence of eosinophils to schistosomula and its schistosomulacidal efficacy were observed. Results Mice immunized with recombinant calpain produced a high level IgG antibodies specific to the antigen immunized. Immunoblotting analysis showed murine anti recombinant calpain sera bound specifically to recombinant calpain. Ninety six percent of schistosomula were surrounded by cells when incubated with mouse eosinophils, and a significant number of dead schistosomula was observed at 48 hours when incubated with mouse serum and eosinophils as compared with control serum and eosinophils ( P

Objective To study the influences of chitosan on swimming time, changes of blood glucose and lactic acid in mice. Methods The test groups were given chitosan by oral administration,and eighteen days later, the swimming time,blood glucose and lactic acid were determined. Results The swimming time of test groups was obviously longer than that of the control group.After thirty-minutes swimming, the elimination of blood lactic acid and the recover of blood glucose were distinctly faster than those of the control group. Conclusion Chitosan had anti-fatigue effect on mice.