OBJECTIVE:To determine the concentration of ozagrel in human plasma by RP-HPLC.METHODS:The sample separation was performed on a SinoChrom OPS-AP column.The mobile phase consisted of 0.025 mol?L-1 potassium dihydrogen phosphate buffer solution-acetonitrile(85∶15) with a flow rate of 0.8 mL?min-1 and a detection wavelength of 274 nm.The internal standard was levodropropizine.RESULTS:The linear range of ozagrel was 0.7～21.3 ?g?mL-1,(r=0.999 6).The extraction recovery of ozagrel was 83.78%～89.14% and the methodological recovery of it was 101.95%～106.68%.The intra-day RSD and inter-day RSD were 1.33%～3.27% and 2.72%～5.97%,respectively.CONCLUSION:This method is simple,rapid,accurate and reproducible,and it is applicable for the pharmacokinetic study of ozagrel in human plasma.

Objective To explore the curative effect of oral cancer radical surgery plus free anterolateral thigh flap repair and analyze the influence factors of skin flap necrosis.Methods The data of 98 patients who underwent oral cancer radical surgery with concurrent free anterolateral thigh flap repair in our hospital from January 2010 to June 2015 were retrospectively analyzed.The complete survival rate of skin flaps and incidence of complications in all patients were statistically analyzed.The age,diabetes,infection in implanted skin flap area,smoking and mental status,etc.of patients with survived or necrotic skin flaps were compared between patients with survival flaps and patients with flap necrosis,and multivariate Logistic regression analysis was performed.Results Among the 98 cases,86 cases of flaps survived completely and the survival rate was 87.8%.Fifteen cases with the skin flap necrosis,the incidence of complications was 15.3%,including 7 cases of surgi-cal site infection and 5 cases of skin flap necrosis with massive hemorrhage.The donor site incisions of all patients were primary healing and the patients were satisfied with the postoperative appearance.Among the patients with flap necrosis,the incidence rates of ≥60 years old,with diabetes,infection in flap implanted area,smoking and poor mental state (41.7%,41.7%,58.3%,75.0%,83.3%)were higher than those corresponding proportions in the patients with with survival flaps(P <0.05).Multivariate Logistic regression analysis showed that age,diabe-tes,infection in implanted skin flap area,smoking and poor mental status were the influencing factors for flap necrosis.Conclusion Oral cancer radical surgery with concurrent free anterolateral thigh flap repair has good curative effects.The age,combined with diabetes,smoking,infec-tion in flap implanted area and poor mental state are the risk factors for flap necrosis in patients with oral cancer after flap repair,which should be given intervention to improve the survival rate of skin flap in clinic.

[Objective]To explore the clinic results of bi-posterolateral fusion combined with coralline hydroxyapatite interbody fusion,a three point stable spine fusion technique in the treatment of degenerative vertebra disc disorder.[Method]Bi-posterolateral fusion combined with posterior interbody fusion were performed to treat 41 cases of degenerative vertebra disc disorder from August 1998 to August 2006.The patients included 18 men and 23 women;their average age was 48 years.The slippage and grade of spondylolisthesis before and after spine fusion were investigated.The severity and slippage of radiographic measurements were also recorded,along with the average follow up of 19.1 month(range 12-48 months).The three point spine fusion applied autologous bone chips for bi-posterolateral fusion and one cubic block of coralline hydroxyapatite for anterior inter body fusion which achieved by PLIF technique.[Result]In the 41 patients who had underwent three point spine fusion surgery,only 1/41 patients experienced nerve root pain after surgery,and in most cases clinical symptoms were improved greatly.In 21/41 patients who had severe lower back pain completely disappeared after surgery,and in 9/41 cases these symptoms were minimal.The clinical evaluation according to the symptoms before the operation was excellent 2.4%(1/41),good 19.5%(8/41),fair 21.9%(9/41),bad 56.1%(23/41);after operation excellent 68.3%(28/41),good 29.2%(12/41),fair 2.4%(1/41),bad 0.0%(0/41).A radiographic measurements showed the anterior disc height was 8.05?2.35mm before operation and 11.44?3.38mm after operation(P

OBJECTIVE:To compare between the HPLC method and gravimetric analysis in the determination of the content of sodium new Houttuyfonte injection.METHODS:The content of sodium new Houttuyfonte injection was determined by HPLC and gravimetric analysis respectively.HPLC condition: Zorba extent-C18 column(250 mm?4.6 mm,5 ?m) detected by UV(286 nm),and the mobile phase consisted of methanol-water-tetrabutyl ammonium hydroxide(70:30:0.3).The procedure of gravimetric analysis was in line with state drug standard.RESULTS: The determination results were notably different between the two methods.CONCLUSION:Gravimetric analysis showed no specificity in the determination of sodium new Houttuyfonte injection.

Objective To find out factors affecting physicians' prescribing conduct so as to get rid of the negative effects and reduce medical costs. Methods Analyses were conducted by reviewing relevant literature published at home and abroad. Results Factors affecting physicians' prescribing conduct include: the medical security system, the hospital compensation mechanism, the requisitioning party, pharmaceuticals per se, and promotion of sales. Conclusion To get rid of the negative effects of physicians' prescribing conduct, it is necessary to rationalize the hospital compensation mechanism, strengthen the control of drug examination, approval, purchase and sale, and provide physicians with accurate pharmaceutical information.

ObjectiveTo investigate the risk factors of early rebleeding after ruptured intracranial aneurysm embolization surgery.MethodsForty-two patients with early postoperative rebleeding after ruptured intracranial aneurysm embolization surgery(study group) were enrolled,and 40 patients without early postoperative rebleeding after ruptured intracranial aneurysm embolization surgery at the same period was selected randomly as control group.Single factor and multiple factors were analyzed by non-condition Logistic regression model.ResultsThe single factor analysis result showed that age ≥50,history of hypertension,diabetes history,ruptured frequency number ≥2,combined with vasospasm,postoperative anticoagulation therapy,postoperative blood pressure fluctuation ≥ 30 mm Hg (1 mm Hg =0.133 kPa),pseudoaneurysm,fatal embolism and most embolism in study group [81.0％(34/42),76.2％(32/42),66.7％(28/42),76.2％ (32/42),81.0％ (34/42),76.2％ (32/42),66.7％ (28/42),66.7％ (28/42),66.7％ (28/42) ]had statistical differences compared with those in control group [57.5％ (23/40),50.0％ (20/40),37.5％( 15/40 ),50.0％ ( 20/40 ),57.5％ ( 23/40 ),47.5％ ( 19/40 ),37.5％ ( 15/40 ),37.5％ (15/40),37.5％ ( 15/40) ](P ＜0.05).Further multiple factors analysis showed that age ≥50,history of hypertension,ruptured frequency number ≥ 2,combined with vasospasm,postoperative anticoagulation therapy,postoperative blood pressure fluctuation ≥ 30 mm Hg were the independent risk factors of early postoperative rebleeding after ruptured intracranial aneurysm embolization surgery (P ＜0.05).ConclusionsAge ≥50,history of hypertension,ruptured frequency number ≥ 2,combined with vasospasm,postoperative anticoagulation therapy,postoperative blood pressure fluctuation ≥ 30 mm Hg are the independent risk factors of early postoperative rebleeding after ruptured intracranial aneurysm embolization surgery.The necessary interventions to these risk factors have important significance,and can lower the incidence of early postoperative ruptured rebleeding after ruptured intracranial aneurysm embolization surgery and improve the prognosis.

As the major commercial source of natural rubber,Hevea brasiliensis attracts much attention.However,the heterozygous nature,long breeding cycle are strong limitations for conventional breeding.While genetic engineering,which can be used to widen the germplasm base and produce desirable agronomic traits quickly and efficiently,offers a viable alternative approach to complement traditional breeding.Comprehensive analysis indicates that in the past two decades,with calli derived from immature anther or integumental tissues of immature fruit as receptors,both biolistic and Agrobacterium-mediated transformation methods were employed for developping rubber genotypes with improved latex yield,tolerance to tapping panel dryness syndrome,producing high-value recombinant proteins,etc.Being recalcitrant to tissue culture,the transformation efficiency of Hevea is comparatively low,and the procedures are still needed to optimize.Finally,breeding objectives and strategies to improve transformation efficiency were also proposed in the review.

Objective · To investigate the histone methyltransferase capability of DOT1L-long form and its role in breast cancer metastasis.Methods · The existence of DOT1L-long form was confirmed by PCR, and the mRNA level of DOT1L was tested by real-time PCR. In HEK293T cells in which DOT1L canonical and DOT1L-long were overexpressed respectively, Western blotting was used to test the expression level of DOT1L and the histone methyltransferase capability. In the MCF10A cell line with inducible expression of DOT1L-long, real-time PCR was used to detect the mRNA level of epithelial-mesenchymal transition (EMT) marker, and transwell assay was used to detect the migration of breast cancer cells in which the expression level of DOT1L is low or high. Results · PCR demonstrated the existence of DOT1L-long form, and real-time PCR showed it widely exists in HCT116, T98G, MCF10A cells, etc. Western blotting showed the expression of DOT1L-long form and its H3K79 methyltransferase activity. In MCF10A cells in which overexpressed canonical DOT1L and DOT1L-long, mRNA levels of N-cadherin and fibronectine increased. Transwell showed canonical DOT1L and DOT1L-long both substantially increased the migration of breast cancer cells. Conclusion · The existence of DOT1L-long was confirmed and investigated, which is 202 amino acids longer than the canonical DOT1L, and is coded by a new exon, located between exon 27 and 28. Further, the DOT1L-long has H3K79 methyltransferase activity, and is able to promote breast cancer metastasis.

Bee/wasp sting associated ischemia stroke is very rare.Its early clinical manifestation,especial level of consciousness,is serious than general cerebral infarction.There is no specificity with laboratory examination.Most of the infarction lesions involve two or two more regions detection by brain computerized tomographic scanning/magnetic resonance imaging (CT/MRI).Parts of the occlusion vessels could recanalization.Therapy with hormones and antihistamine has certain effect.Most of patients have approving prognosis.The pathological mechanism is mainly associated with doctrine such as vascular inflammation,vascular spasm,immune hyperfunction,brain tissue hypoperfusion and retrograde stimulation of the superior cervical ganglion.