Hospitals and their affiliated institutions established by the same organization (hereinafter, Organization A) provide level-specific training programs to cultivate nurses who are independent professionals and continuously perform high-quality nursing practice, but no program has been developed for mid-level nurses. In this study, the current status of continuing education for mid-level nurses and the educational content and teaching method required by mid-level nurses were investigated in order to develop basic educational materials for them. We conducted an anonymous self-administered questionnaire survey of 355 nurses who had ≥10 years of experience in nursing (excluding those in management) and were working at hospitals established by Organization A. A qualitative descriptive analysis of 192 survey responses (response rate, 54.0%) was conducted by simply summarizing qualitative answers and by categorizing written answers through inductive classification in content analysis. The rate of participation in workshops held inside and outside the hospitals in the past 1 year was 92.5% and 69.2%, respectively. When asked about their feeling toward workshops, 56% of the nurses answered by saying “they wanted to attend workshops if attendance was considered as a part of the job”. When asked about whether their preference on educational content and teaching method had been incorporated, 56% of the nurses answered “unsure”. Among the nurses surveyed, 25% did not attend in-hospital workshops because “they were tired” and 24% did not attend out-of-hospital workshops because “the venues were far away”. In addition, 61% of the nurses wanted to attend workshops that help improve professional skills as a mid-level nurse, 54% selected “nursing practice skills” as educational content, and 47% preferred “lecture/practice”, all for the reason that “practice is the mother of all skills”. Our future challenge is to establish a training program for nursing practice skills that teaches how to perform the skills.