1.Identification, synthesis and characterization of an unknown process related impurity in eslicarbazepine acetate active pharmaceutical ingredient by LC/ESI-IT/MS, 1H, 13C and 1H-1H COSY NMR
Thomas Saji ; Paul Kumar Saroj ; Joshi Chandra Subhash ; Kumar Vineet ; Agarwal Ashutosh ; Vir Dharam
Journal of Pharmaceutical Analysis 2014;(5):339-344
A new impurity was detected during high performance liquid chromatographic (HPLC) analysis of eslicarbazepine acetate active pharmaceutical ingredient. The structure of unknown impurity was postulated based on liquid chromatography mass spectrometry using electrospray ionization and ion trap analyzer (LC/ESI-IT/MS) analysis. Proposed structure of impurity was unambiguously confirmed by synthesis followed by characterization using 1H, 13C nuclear magnetic resonance spectrometry (NMR), 1H-1H correlation spectro-scopy (COSY) and infrared spectroscopy (IR). Based on the spectroscopic and spectrometric data, unknown impurity was characterized as 5-carbamoyl-10,11-dihydro-5H-dibenzo[b,f]azepin-10-yl propionate.
2.Epidemiology of patients admitted to a major trauma centre in northern India.
Devarshi RASTOGI ; Sanjay MEENA ; Vineet SHARMA ; Girish Kumar SINGH
Chinese Journal of Traumatology 2014;17(2):103-107
OBJECTIVETrauma in India is an increasingly significant problem, particularly in light of rapid development and increasing motorization. Social changes are resulting in alterations in the epidemiology of trauma. The aim of the study was to assess the various epidemiological parameters that influence the cause of injury in the patients admitted to a major trauma centre in northern India.
METHODSAn observational study of 748 patients chosen by random assortment was carried out over a period of 1 year (August 2008 to July 2009). Age, sex, injury type and pattern were noted. Injury mode of upper and lower limbs was also noted.
RESULTSInjuries occur predominately in the age group of 15-30 years. Males incurred more injury with male to female ratio of 6:1. The most vulnerable group was motorcycle users. Among the injured, farmers were the most commonly involved. Blunt injuries (94.92%) were much more common than penetrating injuries. Among patients with head injury, two-wheeler related accidents were the most common (40.3%). Most spinal cord injuries were caused by falls from height (51.09%). Most lower limb fractures were simple type. Compound fractures of the lower limb were more common than upper limb fractures.
CONCLUSIONStrict enforcement of traffic rules, combined with improved infrastructure and behavior change can decrease the burden of road traffic accidents in India and other developing countries. This study could assist in raising the profile of road traffic accidents as a public health problem which needs to be addressed as a preventable cause of mortality and morbidity, and planning appropriate interventions for this major challenge. Preventive strategies should be made on the basis of these epidemiological trends.
Accidents, Traffic ; statistics & numerical data ; Adolescent ; Adult ; Aged ; Craniocerebral Trauma ; epidemiology ; Female ; Humans ; India ; epidemiology ; Male ; Middle Aged ; Spinal Cord Injuries ; epidemiology ; Wounds and Injuries ; epidemiology
3.Mycoherbicidal Potential of Phaeoacremonium italicum, A New Pathogen of Eichhornia crassipes Infesting Harike Wetland, India.
Birinderjit SINGH ; Sanjai SAXENA ; Vineet MESHRAM ; Maneek KUMAR
Mycobiology 2016;44(2):85-92
Mycoherbicides are exclusive biotechnology products which offer a non-chemical solution to control noxious weeds on the land as well as aquatic in systems, viz a viz saving environment from hazardous impact of synthetic chemicals. The present paper highlights the mycobiota associated with Eichhornia crassipes infesting Harike wetland area of Punjab and evaluation of their pathogenic potential for futuristic application as a mycoherbicide. Of the 20 isolates tested by leaf detached assay and whole plant bioassays, only one isolate (#8 BJSSL) caused 100% damage to E. crassipes. Further, the culture filtrate of this isolate also exhibited a similar damage to the leaves in an in vitro detached leaf assay. The potential isolate was identified as Phaeoacremonium italicum using classical and modern molecular methods. This is the first report of P. italicum as a pathogen of E. crassipes and of its potential use as a biological control agent for the management of water hyacinth.
Biological Assay
;
Biotechnology
;
Eichhornia*
;
Fungi
;
In Vitro Techniques
;
India*
;
Plant Weeds
;
Plants
;
Wetlands*
4.Correlation of fecal calprotectin and patient-reported outcome measures in patients with ulcerative colitis
Nagesh KAMAT ; Sudheer K VUYYURU ; Saurabh KEDIA ; Pabitra SAHU ; Bhaskar KANTE ; Peeyush KUMAR ; Mukesh Kumar RANJAN ; Mukesh Kumar SINGH ; Sambuddha KUMAR ; Vikas SACHDEV ; Govind MAKHARIA ; Vineet AHUJA
Intestinal Research 2022;20(2):269-273
5.Prevalence of hepatitis B, hepatitis C and human immunodeficiency viral infections in patients with inflammatory bowel disease in north India.
Parnita HARSH ; Vipin GUPTA ; Saurabh KEDIA ; Sawan BOPANNA ; Sucharita PILLI ; SURENDERNATH ; Govind Kumar MAKHARIA ; Vineet AHUJA
Intestinal Research 2017;15(1):97-102
BACKGROUND/AIMS: Patients with inflammatory bowel disease (IBD) often require immunosuppressive therapy and blood transfusions and therefore are at a high risk of contracting infections due to hepatitis B (HBV) and hepatitis C (HCV) and human immunodeficiency virus (HIV). In the present study, we assessed the prevalence of these infections in patients with IBD. METHODS: This retrospective study included 908 consecutive patients with IBD (ulcerative colitis [UC], n=581; Crohn's disease [CD], n=327) who were receiving care at a tertiary care center. Ninety-five patients with intestinal tuberculosis (ITB) were recruited as disease controls. Prospectively maintained patient databases were reviewed for the prevalence of HBV surface antigen, anti-HCV antibodies, and HIV (enzyme-linked immunosorbent assay method). HCV RNA was examined in patients who tested positive for anti-HCV antibodies. Prevalence data of the study were compared with that of the general Indian population (HBV, 3.7%; HCV, 1%; HIV, 0.3%). RESULTS: The prevalence of HBV, HCV, and HIV was 2.4%, 1.4%, and 0.1%, respectively, in the 908 patients with IBD. Among the 581 patients with UC, 2.2% (12/541) had HBV, 1.7% (9/517) had HCV, and 0.2% (1/499) had HIV. Among the 327 patients with CD, 2.8% (8/288) had HBV, 0.7% (2/273) had HCV, and 0% (0/277) had HIV. One patient with CD had HBV and HCV coinfection. The prevalence of HBV, HCV, and HIV in patients with ITB was 5.9% (4/67), 1.8% (1/57), and 1.2% (1/84), respectively. CONCLUSIONS: The prevalence of HBV, HCV, and HIV in north Indian patients with IBD is similar to the prevalence of these viruses in the general community. Nonetheless, the high risk of flare after immunosuppressive therapy mandates routine screening of patients with IBD for viral markers.
Antigens, Surface
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Biomarkers
;
Blood Transfusion
;
Coinfection
;
Colitis
;
Colitis, Ulcerative
;
Crohn Disease
;
Hepatitis B*
;
Hepatitis C Antibodies
;
Hepatitis C*
;
Hepatitis*
;
HIV
;
Humans*
;
India*
;
Inflammatory Bowel Diseases*
;
Mass Screening
;
Prevalence*
;
Prospective Studies
;
Retrospective Studies
;
RNA
;
Tertiary Care Centers
;
Tuberculosis
6.Efficacy of Nasobiliary Tubes and Biliary Stents in Management of Patients with Bile Leak after Liver Transplantation: A Systematic Review
Ali RAZA ; Anam OMER ; Sara IQBAL ; Vineet GUDSOORKAR ; Pramoda KODURU ; Kumar KRISHNAN
Clinical Endoscopy 2019;52(2):159-167
BACKGROUND/AIMS: Bile leak is one of the most common complications of liver transplantation. The treatment options for bile leaks include conservative management, surgical re-intervention, percutaneous drainage and endoscopic drainage. We aimed to perform a systematic review to identify the efficacy of endoscopic treatment in the resolution of post-transplant bile leaks. METHODS: Two independent reviewers performed systematic literature search in PubMed, ISI Web of Science, grey literature and relevant references in May 2017. Human studies in English with documented post-liver transplant bile leaks were included. RESULTS: Thirty-four studies were included in the final analysis. The pooled efficacy of biliary stents for the resolution of post-transplant bile leaks was 82.43% compared with 87.15% efficacy of nasobiliary tubes. The efficacy of biliary stents was lower for anastomotic leaks (69.23%) compared to T-tube (90.9%) or cut-surface/ cystic duct stump related leaks (92.8%). Similarly, the efficacy of nasobiliary tube was also lower for anastomotic leaks (58.33%) compared to T-tube or cut-surface related leaks (100%). CONCLUSIONS: In this systematic review, the overall efficacy was 82.43% in biliary stent group, and 87.15% in nasobiliary tube group. Both biliary stent and nasobiliary tube were more effective in managing non-anastomotic leaks compared to anastomotic leaks.
Anastomotic Leak
;
Bile
;
Biliary Fistula
;
Cystic Duct
;
Drainage
;
Humans
;
Liver Transplantation
;
Liver
;
Stents
7.Selective M1 macrophage polarization in granuloma-positive and granuloma-negative Crohn's disease, in comparison to intestinal tuberculosis.
Prasenjit DAS ; Ritika RAMPAL ; Sonakshi UDINIA ; Tarun KUMAR ; Sucharita PILLI ; Nahid WARI ; Imtiaz Khan AHMED ; Saurabh KEDIA ; Siddhartha Datta GUPTA ; Dhiraj KUMAR ; Vineet AHUJA
Intestinal Research 2018;16(3):426-435
BACKGROUND/AIMS: Classical M1 macrophage activation exhibits an inflammatory phenotype while alternative M2 macrophage activation exhibits an anti-inflammatory phenotype. We aimed to determine whether there are discriminant patterns of macrophage polarization in Crohn's disease (CD) and intestinal tuberculosis (iTB). METHODS: Colonic mucosal biopsies from 29 patients with iTB, 50 with CD, and 19 controls were examined. Dual colored immunohistochemistry was performed for iNOS/CD68 (an M1φ marker) and CD163/CD68 (an M2φ marker), and the ratio of M1φ to M2φ was assessed. To establish the innate nature of macrophage polarization, we analyzed the extent of mitochondrial depolarization, a key marker of inflammatory responses, in monocyte-derived macrophages obtained from CD and iTB patients, following interferon-γ treatment. RESULTS: M1φ polarization was more prominent in CD biopsies (P=0.002) than in iTB (P=0.2) and control biopsies. In granuloma-positive biopsies, including those in CD, M1φ predominance was significant (P=0.001). In iTB, the densities of M1φ did not differ between granuloma-positive and granuloma-negative biopsies (P=0.1). Interestingly, higher M1φ polarization in CD biopsies correlated with high inflammatory response exhibited by peripheral blood-derived monocytes from these patients. CONCLUSIONS: Proinflammatory M1φ polarization was more common in colonic mucosa of CD patients, especially in the presence of mucosal granulomas. Further characterization of the innate immune system could help in clarifying the pathology of iTB and CD.
Biopsy
;
Colon
;
Crohn Disease*
;
Granuloma
;
Humans
;
Immune System
;
Immunohistochemistry
;
Macrophage Activation
;
Macrophages*
;
Monocytes
;
Mucous Membrane
;
Pathology
;
Phenotype
;
Tuberculosis*
8.Evaluation of Xpert MTB/RIF assay performance in the diagnosis of abdominal tuberculosis.
Suraj KUMAR ; Sawan BOPANNA ; Saurabh KEDIA ; Pratap MOULI ; Rajan DHINGRA ; Rajesh PADHAN ; Mikashmi KOHLI ; Jigyasa CHAUBEY ; Rohini SHARMA ; Prasenjit DAS ; S DATTAGUPTA ; Govind MAKHARIA ; SK SHARMA ; Vineet AHUJA
Intestinal Research 2017;15(2):187-194
BACKGROUND/AIMS: The use of genetic probes for the diagnosis of pulmonary tuberculosis (TB) has been well described. However, the role of these assays in the diagnosis of intestinal tuberculosis is unclear. We therefore assessed the diagnostic utility of the Xpert Mycobacterium tuberculosis/rifampicin (MTB/RIF) assay, and estimated the prevalence of multidrug-resistant (MDR) TB in the Indian population. METHODS: Of 99 patients recruited, 37 had intestinal TB; two control groups comprised 43 with Crohn's disease (CD) and 19 with irritable bowel syndrome. Colonoscopy was performed before starting any therapy; mucosal biopsies were subjected to histopathology, acid-fast bacilli staining, Lowenstein-Jensen culture, and nucleic acid amplification testing using the Xpert MTB/RIF assay. Patients were followed up for 6 months to confirm the diagnosis and response to therapy. A composite reference standard was used for diagnosis of TB and assessment of the diagnostic utility of the Xpert MTB/RIF assay. RESULTS: Of 37 intestinal TB patients, the Xpert MTB/RIF assay was positive in three of 37 (8.1%), but none had MDR-TB. The sensitivity, specificity, positive predictive value, and negative predictive value of the Xpert MTB/RIF assay was 8.1%, 100%, 100%, and, 64.2%, respectively. CONCLUSIONS: The Xpert MTB/RIF assay has low sensitivity but high specificity for intestinal TB, and may be helpful in endemic tuberculosis areas, when clinicians are faced with difficulty differentiating TB and CD. Based on the Xpert MTB/RIF assay, the prevalence of intestinal MDR-TB is low in the Indian population.
Biopsy
;
Colonoscopy
;
Crohn Disease
;
Diagnosis*
;
Humans
;
Irritable Bowel Syndrome
;
Mycobacterium
;
Nucleic Acid Amplification Techniques
;
Prevalence
;
Sensitivity and Specificity
;
Tuberculosis*
;
Tuberculosis, Multidrug-Resistant
;
Tuberculosis, Pulmonary
9.Bilateral first and second arch anomalies: a rare presentation.
Amit Pal SINGH ; Virad KUMAR ; Vineet NARULA ; Ravi MEHER ; Anoop RAJ
Singapore medical journal 2012;53(4):e74-6
Branchial sinuses are one of the most common congenital anomalies present. They are usually unilateral; bilateral cases are present but are rare. The presentation of bilateral branchial sinus anomalies along with bilateral first arch anomalies is very rare. Here, we present a case of bilateral first arch anomalies co-existing with bilateral second arch anomalies in a patient with no related family history and no associated syndrome.
Adolescent
;
Branchial Region
;
abnormalities
;
surgery
;
Cutaneous Fistula
;
surgery
;
Humans
;
Male
10.Needs monitoring with quetiapine.
Pugazhenthan THANGARAJU ; Sajitha VENKATESAN ; Vineet Kumar CHADHA
Chinese Medical Journal 2019;132(1):113-114