Language ; Medicine, Chinese Traditional ; Terminology as Topic ; Translations

Objective To investigate the effect of hepatocyte growth factor(HGF)on the proliferation of human hepatocellualr carcinoma cells,and the mechanism of HGF-induced proliferation inhibition.Methods Human hepatocellualr carcinoma cell line HepG2 were treated with different concentrations of HGF for different time periods,and the proliferation of these cells was examined by colorimetric BrdU cell proliferation enzyme-linked immunosorbent assay.The expression of S-phase kinase associated protein 2(Skp2)was examined using Western blot and RT-PCR.Plasmids pcDNASkp2 was introduced into HepG2 cells,then the clones showing up-regulation of Skp2 were selected,and the effect of HGF on the proliferation in these clones was investigated.Results HGF inhibited the proliferation of hepatoma cells in a dose and time dependent manner.The expression of Skp2 was significantly suppressed by HGF.Furthermore,HGF did not suppress the proliferation of HepG2 cells transfected with Skp2.Conclusions This study suggests that HGF could inhibit HepG2 cell proliferation,and the down-regulation of Skp2 could be closely related to this suppressed proliferation.

Objective To explore the differences of occupation burnout of the soldiers in southern and northern Xinjiang and explore its related influencing factors.Methods Using stratified randomization sampling method,500 soldiers from south and north of xinjiang military were selected and investigated by Military Job Burnout Scale and Military Social Support Rating Scale.Results ① The military of northern soldiers'accomplishment,somatization,interpersonal relationship,social support score (5.57±3.29,5.53± 3.35,3.38±2.75,9.62± 10.15) was significantly higher than the soldiers who live in south of xinjiang (5.26±3.25,5.13±3.45,2.94±2.39,6.25±5.24) (t=2.091,-2.539,-4.011,7.227,P＜0.05).②Every dimensions of occupation bumout has significant correlation with social support,age,length of military service,marriage,level,working hours,income and job satisfaction (r=0.124,0.220,0.275,0.198,0.192,0.206,0.188,-0.383,P＜0.05).The total score of Military Job Bumout Scale has positive correlation with social support and level (r=0.122、0.410,P＜0.01).③Social support,working hours,job satisfaction and length of military service has forecast effect on the northern military occupation bumout.Social support and level can predict the southern military occupation burnout.Conclusion It is no obviously differences on the general occupational between the north and South Xinjiang Military.Social support is closely related to the job burnout of South and the North military.

Objective To explore the relationship among psychological stress, emotion regulation type and job burnout of military stationed in Xinjiang,and provide theoretical basis for intervening job burn-out in military stationed in Xinjiang.Methods 757 soldiers were chosen by random cluster sampling meth-od and measured with the job burnout scale for military personnel,the psychological stress self-evaluation test and the questionnaire of the soldier’ s emotion regulation type.Results ①The scores of somatization,self-evaluation,passive work slowdown and job burnout of the only-child soldiers ( respectively ( 6.34 ±4.86 ) , (7.66±4.16),(5.00±3.51),(28.60±15.82)) were significantly higher than those of the non-only-child sol-diers(respectively(5.34±4.51),(6.89±4.28),(4.25±3.22),(25.73±15.04) ( t=2.804,2.384,2.950, 2.446;P<0.05 or P<0.01) .For the score of the three factors ( sense of achievement,somatization,and pas-sive work slowdown),as well as the total score of job burnout,soldiers who had injury history were signifi-cantly higher than those who had not ( t=3.471,3.676,4.222,3.469;P<0.01) .The total score and five fac-tors scores of job burnout among soldiers who had high expectation were significantly lower than those soldier who had normal or less expectation ( P<0.05 or P<0.01).② Psychological stress,cognition attention,self comfort,training expectation and emotional appeal had significant predictive effect on job burnout ( t=14. 518,8.241,-4.332,2.990,-2.695;P<0.01).③Cognition attention played a mediating role between psy-chological stress and job burnout,and the rate of mediating effect was 18.8%.Self comfort played a mediating role between psychological stress and job burnout,and the rate of mediating effect was 4.4%.Conclusion -Only-child or not,injury history and training expectation have important influence on job burnout.Cognition attention and self comfort play mediating role between psychological stress and job burnout.

AIM: To study the effects of urocortin(Ucn) on the isolated heart tissues of spontaneously hypertensive rat(SHR).METHODS: Effects of Ucn on contractile force and heart rate were observed in the SHR and Wistar rat right atrium,left atrium and right ventricle strip.RESULTS: Ucn(1-10 nmol/L) concentration-dependently increased the contractile force in the SHR and Wistar rat isolated right atrium.Ucn increased the contractile force in the SHR by(31.1?14.9)% at 3 nmol/L and by(65.7?22.4)% at 10 nmol/L,and its inotropic effect was significantly greater than that in Wistar rat(P

Objective To explore the relationship between high altitude de-adaptation syndrome and training burnout of plateau-garrisoned soldiers after returning to the plain.Methods A total of 140 plateau-garrisoned soldiers who had returned to the plain were chosen by random cluster sampling and measured with the Training Burnout Test.They were divided into two groups according to the occurrence of acute mountain sickness.We compared the differences in training burnout between the two groups and analyzed the relationship between acute mountain sickness and training burnout. Results ①The incidence of high altitude de-adaptation syndrome of plateau-garrisoned soldiers was 80.00% after returning to the plain.There was statistically significant difference between plateau soldiers after returning to the plain,the plateau stability-keeping forces that returned to the plain (78.36%) (χ2 =0.188, P=0.664) and plateau migrants who returned to the plain (75.10%) (χ2 =1.279, P=0.258).Main symptoms of high altitude de-adaptation were fatigue (12.86%), dizziness (11.43%) and meakness (10.00%).②Compared with soldiers who had no high altitude de-adaptation syndrome, victims of de-adaptation syndrome had higher scores of training burnout, physical and psychological exhaustion and training-alienation (P<0.01).Compared with soldiers who had normal body mass indexes, those who were overweight had higher incidence of altitude de-adaptation (P<0.05).③The regression equation between plateau de-adaptation syndrome and training burnout of plateau soldiers after returning to the plain (P<0.01) is:Training burnout =0.498 ×plateau de-adaptation syndrome -0.126 ×age+0.038 ×length of military service +0.069 ×educational degree+0.029 ×body mass index.Conclusion There exist correlations between high altitude de-adaptation syndrome and training burnout of plateau soldiers after returning to the plain.Reducing the occurrence of high altitude de-adaptation syndrome can help reduce the degree of training burnout.

Objective To investigate the relationship among A-type behavior,job burnout and psychosomatic health in grassroots officers and soldiers stationed in Xinjiang, which provides the theoretical basis to maintain their psychosomatic health.Methods Evaluation was carried out with A-type behavior questionnaire, military job burnout scale and simple soldiers psychosomatic health scale in 1 939 grassroots officers and soldiers chosen by random cluster sampling method from army stationed in Xinjiang.Results ①The total scores and each factor scores of the scale had significant positive correlation among the physical scale, the job buruout scale and time hurry (TH) ,competitive and hostility (CH) (r=0.170-0.716, P＜0.01).②Job burnout entered into the physical and mental health regression equation and could explain 68.6％ of the variance(β=0.195, t=32.211, P＜0.01);TH, CH entered the regression equation of job burnout and could explain 29.5％ of the variance(t=4.925,4.427).③ TH indirectly affected physical and mental health through job burnout, the mediating effect of the amount was 0.27 ×0.83=0.2241;CH indirectly affected physical and mental health by job bumout,the mediating effect of the amount was 0.30×0.83 =0.2490.Conclusion Job burnout has fully mediated effect between TH, CH and physical and mental health.

Objective To develop recruits training burnout self-reported questionnaires, and test the reliability and validity.Methods 780 recruits(360 for first test ,420 for second test) in Xinjiang were randomly assesscd with training burnout self-reported questionnaires.Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were applied to the data, and tested the reliability and validity of recruits training burnout self-reported questionnaires.Results The questionnaires consisted of 13 items and 3 dimensions.One of the dimensions (physically and psychologically exhausted situation, training-alienation, low accomplishment) , explained 61.599％ of the total variance.Cronbach's α coefficient of the whole questionnaire was 0.837, Cronbach's α coefficient of each partial scale were between 0.775 and 0.807.Split-half reliability of the whole scale reached to 0.817, split-half reliability of each partial were between 0.758 and 0.793.The correlation coefficient among each dimensions were 0.267 to 0.569 (P＜0.01) ,and the coefficient between each dimensions and the total score were between 0.671 and 0.857(P＜0.01).Confirmatory factor analysis showed that three factor model was optimal (GFI =0.935, AGFI =0.902, NFI =0.907, CFI =0.936, IFI =0.935, RMSEA =0.050).The three dimensions and the total questionnaire had significant positive correlations with mental stress (r=-0.215--0.313, P＜ 0.01) and significant negative correlations with depression(r=0.319-0.602, P＜0.01).Conclusion The recruits training burnout self-reported questionnaires meets the requirements of psychological surveying, and could be used to text recruits training burnout.

Objective To preliminarily investigate the effect of ribosomal protein S7 on apoptosis of HeLa cervical cancer cells.Methods The previously constructed recombinant plasmid pIRES2-EGFP-RPS7 was transfected into HeLa cells,the empty vector pIRES2-EGFP transfected cells as control.Enhanced green fluorescent protein(EGFP)expressing cells were quantified by flow cytometry,and RPS7 protein level was also determined by Western blotting.Cell apoptosis of both RPS7 over-expression cells and knockdown cells were evaluated by flow cytometry after staining using allophycocyanin labeled Annexin-V.Results Apoptotic cell level in the obtained RPS7 transient over-expression HeLa cells was significantly higher than that of vector con-trol cells [(1 0.00 ±0.60)% vs (5.73 ±0.61 )%],with a statistic difference (t =8.63,P =0.001 ). Moreover,the apoptotic level in RPS7 knockdown cells was lower than that in control cells [(3.08 ± 0.49)% vs (5.97 ±0.63)%],with a statistic difference (t =6.40,P =0.003).Conclusion Up-regula-tion of RPS7 may promote apoptosis,while down-regulation of RPS7 may inhibit apoptosis of HeLa cells,indi-cating that RPS7 may play roles in regulating cell apoptosis.

Objective To investigate effects of xeroderma pigmentosum B(XPB) gene on IL-6 induced proliferation and apoptosis in human vascular smooth muscle cells (VSMC).Methods Recombinant plasmid pcDNA3.1-XPB and vacant vector plasmid pcDNA3.1 were transfected stably into VSMC by liposome,and these cells were incubated with IL-6 at a 100 U/ml for 48 hours.The experiments were divided into six groups:blank control group; pcDNA3.1 group; pcDNA3.1-XPB group;IL-6 group; IL-6 + pcDNA3.1 group; IL-6 + pcDNA3.1-XPB group.The expression levels of XPB,Bcl-2,Bax and wild type p53 (wt-p53) were detected through reverse transcription polymerase chain reaction (RT-PCR) and Western blotting.The cell survival,cell cycle and apoptosis were examined with 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry,respectively.Results The transfection of pcDNA3.1-XPB increased the expression of XPB,Bax and wt-p53 (P＜0.05 or P＜0.01),decreased the expression of Bcl-2 (P＜0.05 or P＜0.01),and reduced the IL-6 induced effects on decreasing the expression of Bax and wt-p53 and increasing the expression of Bcl-2(P＜0.05 or P＜0.01).The over expression of XPB inhibited the cell growth(q=2.95,P＜ 0.05),and reduced the positive effects of IL-6 on VSMC growth ( 102.6 +6.2) ％ vs.(124.5 + 7.9) ％,q=3.49,P＜0.05.The over expression of XPB increased the apoptosis rate of VSMC(P＜0.01 ) and the cell amounts of G0/G1 phase (q=2.99,P＜ 0.05),decreased the cell amounts of S phase(q=3.05,P＜0.05),and reduced the IL-6 induced effects on decreasing the apoptosis rate of VSMC(5.9±2.1)％ vs.(0.3±0.1)％,q=7.53,P＜0.01; the cell amounts of G0/G1 phase(70.9±6.7) ％ vs.(54.8±2.9) ％,q=6.91,P＜0.01 ;and on increasing the cell amounts of Sphase(20.2+3.6)％ vs.(36.4+7.2)％,q=8.54,P＜0.01.Conclusions XPB gene could inhibit VSMC proliferation,promote VSMC apoptosis,and reduce the effects that IL-6 promotes VSMC proliferation and inhibits VSMC apoptosis.Therefore,XPB gene is likely to be potential molecular target for treatment of atherosclerosis.