0.05],but patients with grade 2 and 3 had significantly lower Plasma CO levels,[(15.4?1.4),(12.5?1.7)vs(21.1?2.4)mmol/L,P

Objective To investigate the value of intraoperative cholangiography in laparoscopic cholecystectomy(LC). Method Intraoperative cholangiography was attempted in 1226 patients, and was successful in 1180(95.5%). Result Stones were found in the common bile duct in 78 patients, bile duct injury in seven and accessory hepatic duct in two were found.Conclusion Intraoperative cholangiography is a simply and useful procedure in providing clear imaging of biliary system, improving the success rate of LC and reducing the chance of bile duct injury.

BACKGROUND: Personality obstacle is a special type of psychopathy. Since it can reflect personal psychological traits, such as emotion, cognition, behavior mode and the ways one gets along with others, it may lead to meaningful functional impairments and psychological pains if they obviously deviate the normal range, resulting in functional malajustment to society, occupation, social ethics, rules and law. Therefore it deserves further investigation with respect to its criminological significance.OBJECTIVE: To investigate the influence of personality traits, age, educational degree, and marriage state before serving the sentence on criminal behavior of female offenders.DESIGN: A retrospective and control study based on patients.SETTING: Center of psychological sanitary in a hospital of a university.PARTICIPANTS: This study was carried out in the Psychological Sanitary Center of the First Affiliated Hospital of Chongqing Medical College between April 2004 and May 2004. Totally 223 female offenders who incarcerated in Chongqing Female prison were selected. Inclusion criteria: All participants aged from 18 to 60 years old and received above elementary school education. Exclusion criteria: Those who have definite body ailments or psychopaths. Finally totally 204 valid questionnaires were collected from female offenders who were then divided into violent criminal group( n = 120) and non-violent criminal group ( n = 84).METHODS: Totally 223 female offenders who incarcerated in Chongqing Female Prison were asked to fill personality diagnostic questionnaires (PDQ-R), Esonk' personality questionnaires(EPQ), and symptom self-rating chart(SCL-90). Finally totally 204 effective questionnaires were collected for statistical analysis with SAS software.MAIN OUTCOME MEASURES: Comparison of scores for three questionnaires between the two groups; relationship between general condition(age,educational degree and marriage state before incarceration) with the scores for the three questionnaires.RESULTS: The total score for PDQ-R was above 30 in both the two groups and without significant difference between them, ( P ＞ 0.05); the different results for EPQ between the two group was of statistical significance( P ＞ 0. 05); but the total scores for SCL-90 were markedly different between the two groups,( P ＜ 0.01 ); only "educational degree" was found positively correlated with "histrionic trait"; all items except "psychopathy" were close lined with the general state in EPQ questionnaires( P ＜ 0.05); "impulsion and the number of positive items" in SCL-90 were found related with the general condition( P＜ 0.05).CONCLUSION:All criminals have personality obstacle of different type and different degree, but the difference of personality traits between violent female offenders and non-violent female offenders was of no statistical significance.

Objective To explore the effect of extended nursing mainly by stoma therapists on the self-care ability of permanent colostomy patients group.Methods Seventy-eight patients with permanent colostomy were equally randomized by random digits table into control group and observation group.The former received routine discharge education by duty nurses and the latter the extended nursing mainly by stoma therapists. Three months after discharge,the exercise of self-care agency scale (ESCA) was used to conduct the assessments for comparisons of two groups.Result The score on ESCA in the observation group was significantly higher than that of the control group (P<0.01).Conclusion The extended nursing mainly by stoma therapists can improve colostomy patients’quality of life effectively and help them more knowledgeable about the stoma knowledge and skills to enhance self-care ability.

Chromatography, Ion Exchange ; Hemoglobins, Abnormal ; analysis ; Humans ; Male

OBJECTIVE To study the classification of the ?-lactamases produced by Citrobacter freundii.METHODS To detect the ?-lactamases produced by 33 strains of C.freundii with nitrocefin test,a multi-disk test(synergy test and antagonized test) for the inducible AmpC ?-lactamases(IABLs),AmpC ?-lactamases(ABLs) phenotype test,extended-spectrum ?-lactamases(ESBLs) screening test,and carbapenem-hydrolyzing ?-lactamases(CHBLs).RESULTS In the 33 strains,29 strains produced the ?-lactamases(87.9%);the nitrocefin tests of 27 from the 29 strains were positive(81.8%);two strains produced penicillinases,3 strains produced cephalosporinases,5 strains produced broad-spectrum ?-lactamases(BSBLs),11 strains produced ESBLs alone,and 6 strains produced ESBLs+ high-productive ABLs,the rate of ESBLs produced by the 33 strains of C.freundii was 51.5%,and 2 strains produced CHBLs(all produced ESBLs+ metallo-?-lactamase).CONCLUSIONS The rate of ?-lactamases,rate of ESBLs and co-produced rate is very high.There are six kinds of ?-lactamases produced by the 33 strains of C.freundii,mainly BSBLs and ESBLs.

OBJECTIVE To study the classification the ?-lactamases produced by Providencia spp.METHODS To detect the ?-lactamases produced by 19 strains of Providencia spp with nitrocefin test,a multi-disk test(synergy test,antagonized test for the inducible AmpC ?-lactamases(IABLs),AmpC ?-lactamases(ABLs) phenotype test and extended-spectrum ?-lactamases(ESBLs) screening test,carbapenem-hydrolyzing ?-lactamases(CHBLs).RESULTS The nitrocefin tests of all of 19 strains were positive,producing the ?-lactamases(100.0%);eight strains produced broad-spectrum ?-lactamases(BSBLs,42.1%) and ESBLs(42.1%),respectively,one strain produced overexpression of AmpC,3 strains produced CHBLs.CONCLUSIONS The rate of ?-lactamases produced by the 19 strains of Providencia spp is very high,there are three kinds of ?-lactamases,mainly BSBLs and ESBLs.

OBJECTIVE To have a research on the detection of Mycobacterium tuberculosis by fluorescence chip of molecular beacon probe.METHODS Fluorescence chip for the detection of M.tuberculosis was designed,based on the better bonding force and specificity of molecular beacon and target molecule.Hybridization efficiency was optimized,which included the concentration and purity of molecular beacon probe,hybridization temperature,hybridization time,the prescription of hybridization solution and the comparing of molecular beacon fluorescence signals of 4 molecules.RESULTS Molecular beacon fluorescence signal approached the highest when the final concentration of Mg was 5 mmol/L and the amplification efficiency of PCR approached the highest.Half extended molecular beacon probe could bind nice with chip when it was middle labeled with quench group.The results of 3 hybridization solutions were as follows: 0.15% SDS better than 0.2% SDS,10?SSC better than 5?SSC.Strong signal of hybridization reaction could be detected when the concentration of Cy3-BDH molecular beacon probe was 15 ?mol/L,the concentration of FAM-DABCLYE probe was 9 ?mol/L and the reaction time was 7 h.The efficiency of fluorescence quenching was higher when the molecule was consisted of Cy3-BDH,other than Cy3-DABCLYE.CONCLUSIONS The design of half extended molecular beacon probe tactfully makes use of the advantages of the high bonding force and specificity of molecular beacon probe on the chip technology.Fluorescence chip of molecular beacon for M.tuberculosis has satisfactory detecting rate,specificity and sensibility.

Aim: Exploration of ways to train young surgical teachers' teaching ability Method: organizing medical training courses;establishing expert training commission;establishing subject teaching group to give guidance;Improving the estimating quality systems of internet education.Result:To relieve the tension of the shortage of teachers caused by enlarged enrollment and heavy teaching tasks in order to adapt to the current rapid development of medical education Conclusion:Pertinent training will be an effective way to improve the young teachers' teaching consciousness,mental diathesis and ability.