1.Rotavirus vaccine and health-care utilization for rotavirus gastroenteritis in Tsu City, Japan
Kazutoyo Asada ; Hajime Kamiya ; Shigeru Suga ; Mizuho Nagao ; Ryoji Ichimi ; Takao Fujisawa ; Masakazu Umemoto ; Takaaki Tanaka ; Hiroaki Ito ; Shigeki Tanaka ; Masaru Ido ; Koki Taniguchi ; Toshiaki Ihara ; Takashi Nakano
Western Pacific Surveillance and Response 2016;7(4):21-36
Background: Rotavirus vaccines were introduced in Japan in November 2011. We evaluated the subsequent reduction of the health-care burden of rotavirus gastroenteritis.
Methods: We conducted active surveillance for rotavirus gastroenteritis among children under 5 years old before and after the vaccine introduction. We surveyed hospitalization rates for rotavirus gastroenteritis in children in Tsu City, Mie Prefecture, Japan, from 2007 to 2015 and surveyed the number of outpatient visits at a Tsu City clinic from 2010 to 2015. Stool samples were obtained for rotavirus testing and genotype investigation. We assessed rotavirus vaccine coverage for infants living in Tsu City.
Results: In the pre-vaccine years (2007-2011), hospitalization rates for rotavirus gastroenteritis in children under 5 years old were 5.5, 4.3, 3.1 and 3.9 cases per 1000 person-years, respectively. In the post-vaccine years (2011-2015), the rates were 3.0, 3.5, 0.8 and 0.6 cases per 1000 person-years, respectively. The hospitalization rate decreased significantly in the 2013-2014 and 2014-2015 seasons compared to the average of the seasons before vaccine introduction (p < 0.0001). In one pre-vaccine year (2010-2011), the number of outpatient visits due to the rotavirus infection was 66. In the post-vaccine years (2011-2015), the numbers for each season was 23, 23, 7 and 5, respectively. The most dominant rotavirus genotype shifted from G3P[8] to G1P[8] and to G2P[4]. The coverage of one dose of rotavirus vaccine in Tsu City was 56.5% in 2014.
Conclusion: After the vaccine introduction, the hospitalization rates and outpatient visits for rotavirus gastroenteritis greatly decreased.
2.Serotype and Nucleotide Analysis of Human Rotavirus Isolates in Korea.
Kwang Jong CHA ; Jin Ook SONG ; Hong Chan CHO ; Yong Hee KIM ; Dae Yeul YU ; Joong Bok LEE ; Chong Kee LEE ; Taniguchi KOKI ; Jae Hyeun YU
Journal of the Korean Society of Virology 1999;29(2):75-86
Rotaviruses belong to Reoviridae causes diarrhea in human beings as well as domestic animals. This study was conducted to see what type of human rotaviruses are distributed in Seoul and Kyung-gi province. Twenty two of 81 patients showed rotavirus positive with diagnostic kit and RNA electropherosis. We isolated all of rotaviruses from the patients. Electropherotypes of 22 isolates showed 4:2:3 :2 patters whereas those migration patterns were long type. All of those isolates belonged to group 4. Twenty out of 22 isolates reacted with monoclonal antibodies specific to G1, P1A and subgroup II, whereas rest of them, 4-29 and K-30 reacted with subgroup I specific monoclonal antibody. The nucleotide sequence of an isolate K-21 showed 98~100% and 90~96% homologies with those of Wa and KU strain, respectively.
Animals, Domestic
;
Antibodies, Monoclonal
;
Base Sequence
;
Diarrhea
;
Gyeonggi-do
;
Humans*
;
Korea*
;
Reoviridae
;
RNA
;
Rotavirus*
;
Seoul
3.Serotype and Nucleotide Analysis of Human Rotavirus Isolates in Korea.
Kwang Jong CHA ; Jin Ook SONG ; Hong Chan CHO ; Yong Hee KIM ; Dae Yeul YU ; Joong Bok LEE ; Chong Kee LEE ; Taniguchi KOKI ; Jae Hyeun YU
Journal of the Korean Society of Virology 1999;29(2):75-86
Rotaviruses belong to Reoviridae causes diarrhea in human beings as well as domestic animals. This study was conducted to see what type of human rotaviruses are distributed in Seoul and Kyung-gi province. Twenty two of 81 patients showed rotavirus positive with diagnostic kit and RNA electropherosis. We isolated all of rotaviruses from the patients. Electropherotypes of 22 isolates showed 4:2:3 :2 patters whereas those migration patterns were long type. All of those isolates belonged to group 4. Twenty out of 22 isolates reacted with monoclonal antibodies specific to G1, P1A and subgroup II, whereas rest of them, 4-29 and K-30 reacted with subgroup I specific monoclonal antibody. The nucleotide sequence of an isolate K-21 showed 98~100% and 90~96% homologies with those of Wa and KU strain, respectively.
Animals, Domestic
;
Antibodies, Monoclonal
;
Base Sequence
;
Diarrhea
;
Gyeonggi-do
;
Humans*
;
Korea*
;
Reoviridae
;
RNA
;
Rotavirus*
;
Seoul
4.Isolation, Serotyping and Nucleotide Sequence Analysis of Bovine Ratavirus Isolated from Korean Native Cattle.
Jae Hyeun YU ; Kwang Jong CHA ; Eung Ryool KIM ; You Seong KIM ; Young Kun LEE ; Jin Ook SONG ; Hong Chan CHO ; Ji Sun JU ; Bum Suk PARK ; Dea Hwan YOO ; Se Min KIM ; Byoung Jun JI ; Joong Bok LEE ; Shozo URASAWA ; Taniguchi KOKI ; Harry B GREENBERG
Journal of the Korean Society of Virology 2000;30(3):189-202
No Abstract Available.
Animals
;
Base Sequence*
;
Cattle*
;
Serotyping*