Previously we reported effects of acupuncture to the stellate ganglion on measurements of skin electroconductivity by a Neurometer.
By using 8 healthy adults and 23 patients, we measured effects of electrical acupuncture to the stellate ganglion on carotid blood flow, deep tissue temperature, blood pressure and pulse rate in order to clarify the influence on functions of the autonomic nervous system.
After electrical acupuncture to the right stellate ganglion (SGA), the right and left carotid blood flow decreased with 4-9% and 7-12%, respectively. Deep tissue temperature of the right anterior forearm after SGA showed a little increase (with no significance), while the temperature increased with 0.4-0.7°C after the right stellate ganglion block (SGB). Deep tissue temperature of the left anterior forearm showed no significant change after SGA, while it increased with 0.1-0.3°C significantly after SGB. Systolic blood pressure increased with 2-4mmHg after SGA and this also increased with 9-11mmHg after SGB. Those increases were significant. While the pulse rate decreased (2bpm) significantly after SGA, it increased (4-6bpm) significantly after SGB.
Except the decreased pulse rate after SGA, the other data did not support a common hypothesis that electrical acupuncture to the stellate ganglion suppresses the sympathetic system as SGB.

Previously we reported that acupuncture to the stellate ganglion stimulates sympathetic nervous system while it decreases heart rate.
As it is said that R-R intervals in electrocardiogram (ECG) reflect functions of parasympathetic nervous system, we measured effects of electrical acupuncture to the stellate ganglion (SG) or traditional acupuncture points (AP) on the meridians (H7: SHENMEN and P4: HSIMEN) on R-R intervals in ECG by using 24 patients; 12 each in SG-group and AP-group.
Results are as follows; (1) Means of heart rate (HR) decreased with 1-3bpm in the both groups; (2) Coefficients of variation (CV) of HR increased with 1-2% in the both groups; (3) Means of R-R intervals prolonged with 39-47msec in the SG-group and 20-44msec in the AP-group, respectively; (4) CV of R-R intervals increased with 1% in the SG-group only.
It is suggested that acupuncture stimulates not only sympathetic nervous system, but also parasympathetic nervous system.

An accidental break of an acupuncture needle is one of the most serious complication in acupuncture.
A German Shepherd weighed 25kg was anesthetized with pentobarbital.
Then, unused 7 needles of 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0cm long each were inserted at 7 acupuncture points.
The movement of broken acupuncture needles were observed by X-ray for 72 days and pathological examination of tissue surrounding broken needles was also done.
While broken needles inserted nearby the joints were found moving much around, others inserted in the region of straight bones stayed almost.
The dog was dissected 72 days after the preparation. Three broken needles were found just beneath the muscular fascia and two broken needles were in the subcutaneous tissue. Another broken needle in the lumbar region was penetrated into the lumbar spinal cord and the other needle in the region of neck was lost.
Pathological examination showed chronic inflammatory changes such as cellular infiltration and muscle fiber necrosis in the lumbar spinal cord and the thigh region, but little changes in the other four regions.

On twenty patients and ten healthy adult volunteers, pain threshold was measured by a dolorimeter (Pain meter NYT-5) to clarify whether analgesic effects of electrical acupuncture on various body surface may be changed by acupuncture points or not.
Electrical acupuncture was performed to the traditional acupuncture points on the two meridians. A group is the combination of LI 10 (Shousanli) and LI 14 (Hoku) on the right side, the other is the combination of ST 36 (Tsusanli) and ST 40 (Liangchiu) on the right side.
Measured points of pain threshold are (1) face, (the point 2cm above from the center of both eyelids), (2) (3) left and right upper extremities (LI 11; Chuchih) and (4) (5) left and right lower extremities (ST 41; Chiehhsi).
In the Shousanli-Hoku group, pain thresholds of face, both upper extrimities increased significantly. However, those of both lower extremities were not significantly changed.
In the Tsusanli-Liangchiu group, pain thresholds of the upper and lower extremities on both sides increased significantly. However there were no significant changes in the forehead.
In comparison of two groups, increases of pain thresholds of both lower extremities were observed in significantly more cases with acupuncture to Tsusanli and Liangchiu points than those with Shousanli and Hoku points by the Chi-square test (p<0.01). From this fact, acupuncture of Tsusanli-Liangchiu points seems to be more effective on the lower extremities than that of the Shousanli-Hoku points.
Therefore, we conclude that effects on pain threshold by electrical acupuncture depend on various points on meridians and are not evenly shown on the whole body surface.

It is said that Ryodoraku reflects the dysfunction of the autonomic nerve in spite of no objective evidence for the theory.
Using 60 patients received right stellate ganglion block, we measured 24 typical determinate points of ryodoraku with a neurometer and an electrodermometer.
With a neurometer, an average current value of 6 points of the right upper extremity decreased in 52% as well as that of 6 points of the left decreased in 62%. Both the right and the left lower extremities decreased in 91% and 85%.
With an electrodermometer, the average impedance of the right upper extremity increased in 108%.
Finally, the sympathetic blockade showed decrease in a current value with a neurometer and increase in impedance with an electrodermometer.

It has been told that acupuncture to the stellate ganglion suppresses the sympathetic activity. By using 15 patients, we made measurements of electrical current of the skin at the 24 typical determinate points of Ryodoraku with a Neurometer before and after electrical acupuncture to the right stellate ganglion.
After 20min rest in the supine position, average values of 6 points of the right and left upper extremities decreased with 30% and 27%, and those of the right and the left lower extremities decreased with 34% and 31%, respectively.
Immediately after 30min of electrical acupuncture, average values of 6 points of the right and left upper and the right and left lower extremities increased with 9%, 12%, 17% and 9%, respectively.
Twenty minutes after the right stellate ganglion block, average values of 6 points of the right upper extremity decreased with 16%, and those of the left one, the right and left lower extremities decreased with 9%, 18% and 14%, respectively.
These data suggest that acupuncture to the stellate ganglion stimulates the sympathetic tone by showing increase of electrical conductivity of the skin, while the stellate ganglion block shows the decrease.

Objective: To optimize aluminum packaging used for medicinal products based on universal design (UD), the material properties of this packaging were evaluated.  In addition, a questionnaire on the usability of this packaging was conducted for the same purpose.
Methods: Tear resistances of the films used for both aluminum and polypropylene packaging were measured using a tensile tester. To evaluate tear linearity of both packaging types, gap widths from standard line to tear line were measured after the samples were torn 75 mm in length.  A survey of 480 pharmacists was conducted on usability.
Results: Regarding material properties, the maximum value of tear resistance for aluminum packaging was approximately 28 times higher than that for polypropylene packaging.  The maximum gap width from standard line to tear line was approximately 10 mm for both types of packaging.  Regarding the questionnaire, the total response rate was 41.0%.  In valid responses, approximately 90% of pharmacists experienced frustration when opening aluminum packaging and requested improvements in usability. In addition, 88% of pharmacists opened packaging in the longitudinal direction by hands.  For the important point in usability of aluminum packaging, the following answers were confirmed: “speed of opening (43%),” “ease of tearing (31%)” and “ease of taking blisters out (20%).”
Conclusion: Our results suggest that current aluminum packaging is difficult to open and tear linearly based on the evaluation of material properties.  These points are consistent with the concerns highlighted in the survey of pharmacists.  We therefore conclude that the points to be improved when considering UD are the ease of opening and linear tearing for more efficient use of aluminum packaging in a clinical setting.

Previously we reported that acupuncture to the stellate ganglion stimulates the parasympathetic nervous system as well as the sympathetic nervous system. We measured effects of laser acupuncture to the stellate ganglion (SGL) or electrical acupuncture to the same (SGA) on R-R intervals, deep tissue temperature and blood presure by using 45 patients; 12 in SGL-group and 33 in SGA-group.
Results are as follows; (1) Means of heart rate (HR) decreased with 2bpm in the SGL-group, 3bpm in the SGA-group. (2) Coefficient of variation (CV) of R-R intervals increased with 1% in the SGA-group only. (3) Deep tissue temperature of the ipsilateral anterior forearm of the stimulation did not increase significantly after SGA, while deep tissue temperature increased with 0.3-0.5°C after SGL. Deep tissue temperature of the contralateral anterior forearm showed no significant change in the both groups. (4) Systolic blood pressure increased with 2-4mmHg after SGA and 4-5mmHg after SGL, respectively.
It is suggested that SGL increases deep tissue temperature by affecting autonomic nervous system, but not directly.

This study was performed to clarify whether local anesthesia or sympathetic ganglion block abolishes the effects of electrical acupuncture (EA) on the autonomic nervous system or not.
Twelve healthy adult volunteers and 24 patients are divided into the following three experimental groups of each 12. EA was performed to the traditional acupuncture points on the meridians (H7; SHENMEN and CX4; HSIMEN). (1) In control group, only EA was performed. (2) In stellate ganglion block (SGB) group, EA was performed after unilateral SGB. (3) In local anesthesia (LA) group, EA was performed after local anesthesia on the same acupuncture points. The measured parameters on the autonomic nervous system functions were heart rates, R-R intervals in ECG, blood pressure and deep tissue temperature.
Results are as follows; Means of heart rate decreased significantly by EA in both control and SGB groups. Systolic blood pressure, diastolic blood pressure and RR-CV showed no significant changes by EA in all groups. Deep tissue temperature of the anterior forearm ipsilateral to the stimulation side decreased significantly by EA in SGB group. Deep tissure temperature of the anterior forearm contralateral to the stimuli decreased significantly by EA in both control and SGB groups. In LA group, however, all parameters were not changed by EA.
In summary, the effects of electrical acupuncture on the autonomic nervous system were not much different between control and SGB groups. It seems that one side of stellate ganglion block dose not interfere with the effects of acupuncture. On the other hand, any changes on the autonomic nervous system by electrical acupuncture were not observed in LA group. The fact showed that local anesthesia abolishes the effects of acupuncture on site. Local anesthesia blocks peripheral nerves and nerve endings. Therefore, we conclude that the effects of acupuncture on the autonomic nervous system are transmitted through the peripheral nerves, especially through the sensory nerves.