Background: Helicobacter pylori infection is difficult to diagnose in children, especially in developing countries where non - invasive methods such as urea - breath test are often not available. Objectives: To evaluate the sensitivity and specificity of a new monoclonal antibody - based antigen - in - stool enzyme immunoassay (Premier Platinum HpSA PLUS) for diagnosis of H. pylori infection in Vietnamese children. Subjects and method: Sensitivity of the antigen - in - stool test was evaluated in 232 children, 3 - 15 years of age, who were positive for H. pylori infection by culture from biopsies. For evaluation of the specificity 241 children of similar age with non - gastrointestinal conditions were included with blood and stool samples. Results:Among the culture - positive children, 224 were also positive by Premier Platinum HpSA PLUS. Among the control children 98 had negative for H. pylori infection as established by serology, using two ELiSAs (in house and Pyloriset EIA - Gill) and an immunblot (Helicoblot 2.1). These 93 childrenhad H. pylori negative also in the stool test. The sensitivity, specificity, and positive and negative predictive values of Premier Platinum HpSA PLUS were thus found to be 96.6%, 94.9%, 97.8%, and 92.1 %, respectively. Conclusion: The outcome have demonstrated Premium Platinum HpSA PLUS to be a reliable method for detection of H. pylori infection in Vietnamese children.
Helicobacter pylori/ pathogenicity ; Child

Background: Continuation of previous study of screening traditional medicinal plants with anti-diabetic effect, the hypoglycemic effect of glue extracted from Fructus Lycii which was named HPD was study. Objective: to investigate the hypoglycemic effect of HPD on diabetic mice caused by different doses of streptozocine. Subjects and methods: causing diabetes by streptozocine at difference doses was used to collect glucose intolerance mice, mild diabetic mice and severe diabetic mice. The effect of HPD p.o. at several doses was evaluated after 3 days of use. Results: By oral allocation with the dose of 500 and 1.000mg/kg body-weight for 3 days, HPD inhibited increasing blood glucose in streptozocine-induced glucose intolerance mice (intraperitoneal STZ 100 mg/kg). HPD 1000 mg/kg body weight had the potent hypoglycemic effect on streptozocine-induced diabetic mice, reverse the blood glucose self control ability of mild streptozicine-induced diabetic mice (STZ 120 mg/kg), meanwhile can not stop a hyperglycemic effect of high dose of STZ (STZ 180 mg/kg). Conclusion: HPD has the potent anti-hyperglycemic effect on streptozocine -induced diabetic rats. Anti-hyperglycemic of HPD is indirect under present of insulin\r\n', u'\r\n', u'\r\n', u'
Cornus ; Mice ; Inbred NOD ; Streptozocine ;

Anaplasma marginale and A. platys were detected and characterized (16S rDNA sequence analysis) from dairy and indigenous cattle, and the latter in domestic dogs in Vietnam. A phylogenetic tree was inferred from 26 representative strains/species of Anaplasma spp. including 10 new sequences from Vietnam. Seven of our Vietnamese sequences fell into the clade of A. marginale and 3 into A. platys, with strong nodal support of 99 and 90%, respectively. Low genetic distances (0.2–0.4%) within each species supported the identification. Anaplasma platys is able to infect humans. Our discovery of this species in cattle and domestic dogs raises considerable concern about zoonotic transmission in Vietnam. Further systematic investigations are needed to gain data for Anaplasma spp. and members of Anaplasmataceae in animal hosts, vectors and humans across Vietnam.
Anaplasma marginale ; Anaplasma ; Anaplasmataceae ; Animals ; Asian Continental Ancestry Group ; Cattle ; DNA, Ribosomal ; Dogs ; Humans ; Phylogeny ; Trees ; Vietnam