AIM:To establish an HPLC method for determining geniposide and liquiritin in Zhizi Baipi Decoction(Fructus Gardeniae,Cortex Phellodendri Chinensis,Radix et Rhizoma Glycyrrhizae). METHODS: A Kromasil C_(18) Column(4.6 mm?250 mm,5 ?m) was used.The mobile phase was methanol-0.5% glacial acetic acid for gradient elution.The flow rate was 1.0 mL/min,?_1=238 nm(detect the geniposide),?_2=276 nm(detect the liquiritin).The column temperature was at 30 ℃. RESULTS: The linear range of geniposide was within 49.1 ?g/mL-147.4 ?g/mL,r=(0.999 8),the recovery was(99.40%)(n=9).The linear range of liquiritin was within 6.1 ?g/mL-18.4 ?g/mL,r=(0.999 9),the recovery was 99.94%(n=9). CONCLUSION: This method has good reproducibility.It can be used for quality control in the production of Zhizi Baipi Decoction.

Objective To establish a HPLC method for determination of hypoxanthine and xanthine in Syngnathus.Methods A Lichrosper C_(18) Column was used.The mobile phase was methanol-0.1%HAc.Walvelength was 254nm.Results The linear range of hypoxanthine was within 5.91～94.56?g?mL~(-1),r=0.9999,sample recovery rate was 99.22%,RSD=(1.25)%.The linear range of Xanthine was within 2.04~32.64?g?mL~(-1),r=0.9998,sample recovery rate was 98.05%,RSD=1.21%.Conclusion This method has good repeatability and flexibility.It can be used for quality control in production of Syngnathus.

AIM: To establish a HPLC for determination of puerarin and baicalin in Gegen Qinlian Micropill. METHODS: A Kromasil C 18 Column was used. The mobile phase was methanol-0.2% phosphoric acid. Puerarin and baicalin were determined by dual wavelength, ? 1=250nm. ? 2=315nm. RESULTS: The linear range of puerarin was within 32.0 ng—288.0 ng,r=0.9998 and the sample recovery was 100.17 % (RSD=1.00%(n=9)). The linear range of baicalin was within 52.6 ng～447.1 ng, r=0.9999 and the sample recovery was 100.06 % (RSD=1.06% (n=9)). CONCLUSION: This method has good repeatability and flexibility. It can be used for quality control of Gegen Qinlian Micropill.

AIM: To establish a HPLC for determination of baicalin and emodin in Yiqing Capsule(Radix Scutellarial, Radix et Rhizoma Rhei). METHODS:A Kromasil C_(18) Column was used. The mobile phase was methanol -0.2% phosphoric acid for gradient elution. Baicalin and emodin were determined by dual wavelength, ?_1=315 nm, ?_2=266 nm. RESULTS:The linear range of baicalin was within 64.40-322.00 ?g?mL~(-1) (r=(0.999 8)). The average recovery was 99.96%,RSD was 1.57%(n=9). The linear range of emodin was within( 0.82)-13.20 ?g?mL~(-1)(r=0.999 9).The average revovery was 98.90%, RSD was 1.25%(n=9). CONCLUSION: This method has good repeatability and flexibility. It can be used for quality control in production of Yiqing Capsule. (Nan

AIM: To establish a HPLC method for determination of puerarin and paeoniflorin in Jingfukang Granules(Radix Puerariae, Radix Paeoniae Alba, etc.). METHODS: A Kromasil C 18 Column was used. The mobile phase was methanol water. Puerarin and paeoniflorin were determined by HPLC(dual wavelength, ? 1=250nm,? 2=230nm). RESULTS: The linear range of puerarin was within 99.6ng～996.0ng, r =0.9998, sample recovery was 100.69%, RSD =1.04%( n =9), respectively. The linear range of paeoniflorin was within 119.6ng～ 1315.6 ng, r =0.9999, sample recovery was 100.30%, RSD =1.24%( n =9), respectively. CONCLUSION: This method is simple, reliable and has good repeatability. It can be used for quality control of Jingfukang Granules in production.

Objective To investigate the expressions of Dapper1 in gastric carcinoma and elucidate its relationship with survivin and its role in tumor cell apoptosis. Methods Dapper1 mRNA was detected with RT-PCR using specimens from 30 cases of gastric carcinoma and the corresponding normal gastric mucosa.The pcDNA3.1-Dpr1 plasmid was transfected into SGC-7901 cells with LipofectamineTM 2000.The effect of upregulation of Dpr1 on SGC7901 cell apoptosis was determined by flow cytometry.The downregulation of survivin、Dvl-2 and β-catenin protein expression were detected by Western blot analysis.Results Downregulation of Dpr1 gene expression was observed in 17(57%)of 30 human gastric cancer and the downregulation was significantly correlated with the depth of invasion and the degree of differentiation (P＜0.05).Also,upregulation of Dpr1 mRNA and downregulation of survivin mRNA were detected after transfecting pcDNA3.1-Dpr1 plasmid in SGC7901 cells,which led to downregulation of survivin、Dvl-2、β-catenin protein and increase of the SGC7901 cell apoptosis rate from 2.89%to 13.96%.Conclusion Downregulation of Dp1l gene expression is common in human gastric carcinoma,and upregulation of Dpr1 results in significant inhibition of survivin expression which can induce apoptosis of SGC7901 cells.

Objective This study was to investigate the relationship between the activities of thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD) and clinicopathological parameters in human colorectal cancer,and the relationship between TP and/or DPD activity in tumor tissue and efficiency of chemotherapy. MethodsSixty-eight patients undergoing surgery for primary colorectal cancer were enrolled including 40 patients receiving postoperative adjuvant chemotherapy with 5-FU plus leucovorin(de Gramont regimen). The activities of TP and DPD both in tumor tissue and in normal tissue were determined by enzyme-linked immunosorbent assay(ELISA). Results The TP activity was significantly higher in tumor than in normal tissues(P

Objective To study the influence of transferring a dominant-negative Stat3 gene, Stat3?on colon cancer cells' proliferation and apoptosis in vitro. Methods Cell culture of human colon cancer cell line SW480 and transient transfection were used to evaluate the effect of transferring Stat3?to cancer cells. Cell proliferation, cell cycle and apoptosis were quantified by MTT and flow cytometry, respectively. The mRNA expression of Stat3's target gene cyclin D1 and bcl-xL was detected by reverse transcription polymerase chain reaction. Independent t tests were used for data statistics. Results 36 h after Stat3?plasmids transfection, proliferation of SW480 cells was significantly inhibited (t =5. 216,P = 0.006); cell proportion of G0/G1 phase increased from 40.37% to 67.25% and early apoptosis cells increased from 5. 34% to 24. 42% ; mRNA expression of cyclin Dl and bcl-xL declined significantly (t = 5.288,P=0.010;t=3.517,P=0.025). Conclusion Blocking Stat3 signaling pathway by transfection of Stat3?plasmid inhibits the proliferation and promotes apoptosis of colon cancer cells, which provides a experimental foundation of Stat3 targeted colon cancer gene therapy.

Objective:To investigate the application of in vitro fenestration endovascular aortic repair(fEVAR) in the juxtarenal abdominal aortic pseudoaneurysm and its up to mid-term results.Methods:The clinical data of 5 cases of juxtarenal abdominal aortic pseudoaneurysm from Oct 2016 to Jul 2019 at the Department of Vascular Surgery, Henan Provincial People′s Hospital was retrospectively analyzed, including therapy options, accesses, techniques of fenestration, bundle of the stent-graft, near to medium-term effects.Results:All patients were treated with fEVAR, the technical success rate was 100%. Stent modify time ranged from 50 to120 minutes, fEVAR time ranged from 75 to 210 minutes. The follow-up period was 15~42 months. All of the stents are in good position, there is no stent-related complications, and no deaths. Primary diseases are well controlled.Conclusion:The treatment for juxtarenal abdominal aortic pseudoaneurysms with fEVAR , as a full-intraluminal method, is of minimal invasion, few perioperative complications, low mortality. Result of up to mid-term follow up is satisfactory.

0.05) but a statistically significant negative correlation was noted between serum cholesterol and zinc (r= -0.9986, P