Aim To investigate the inhibitory effect of Evodiamine on JAK2/STAT3 signal pathway in human colorectal cancer cell line HCT-116 . Methods Cells were cultured with 6. 0 μmol·L-1 Evodiamine for 2, 4 and 6 h, respectively. Cell nuclear morphology was detected by Hoechst staining and protein expression levels of JAK2 , p-JAK2 , STAT3 and p-STAT3 were examined by Western blot. Cells were treated with dif-ferent concentrations of AG490 for 48 h to select proper working concentration and cells treated with 6 μmol · L-1 EVO and 50 μmol · L-1 AG490 to compare the modulatory effect of EVO with AG490 on JAK2/STAT3 signal pathway. Results Hoechst staining revealed that Evodiamine could induce cells apoptosis, chroma-tin condensation gathered and typical apoptotic mor-phological changes in a time-dependent manner;West-ern Blot suggested that EVO could inhibit p-STAT3 significantly. After treatment with AG490, JAK2/STAT3 signal pathway was inactivated, the inhibitory effect of EVO on p-STAT3 was stronger than that of AG490 , while EVO combined with AG490 could fur-ther inhibit the expression of p-STAT3 significantly. Conclusions The anticancer effect of Evodiamine is mainly mediated by the modulation of JAK2/STAT3 signal pathway in HCT-116 cells.

Objective To analyze the status of head circumference development and related factors of microcephaly among infants in Chongqing, China. Methods We included infants aged 7-24 months who had received child health care services at a Chongqing hospital from 1 February 2010 to 1 November 2014. We collected data of sex, age, head circumference, childbirth conditions, mother's pregnancy history, infant feeding patterns, and parents' educational level. Ultimately, 31 902 infants (17 511 males and 14 391 females) were analyzed. The t-test was used to compare head circumference by sex, and logistic regression models used to analyze the related factors of microcephaly. Odds ratios (OR) and 95%confidence intervals (CI) were calculated. Results Among the different age groups (7-8, 9-10, 11-12, 13-18, and 19-24 months), head circumferences for males were (44.3±1.3), (45.2±1.4), (46.1±1.5), (46.8±1.4), and (47.8±1.4) cm, respectively;for females, head circumferences were (43.1±1.3), (44.1±1.3), (44.8±1.4), (45.7±1.4), and (46.7±1.3)cm, respectively. Head circumference for males were all greater than those for girls (t-values were 32.09, 27.73, 32.21, 41.66, and 32.03, respectively; P<0.001). The rate of microcephaly was 3.2%(1 025/31 902) among infants. Birth weights and fetal times were related to microcephaly in infants, with OR (95%CI) values 0.15(0.13-0.18) and 1.31(1.11-1.54), respectively. Factors including urban location, delayed birth, cesarean section, formula feeding, and mixed feeding patterns might reduce the risks of microcephaly compared with suburban location, term birth, eutocia, and breastfeeding; OR (95% CI) values were 0.60 (0.52-0.68), 0.55 (0.44-0.70), 0.76 (0.67-0.87), 0.46 (0.32-0.67), and 0.51 (0.34-0.75), respectively. Conclusion Head circumference development among infants in Chongqing was satisfactory. Family environmental factors, birth conditions, and feeding patterns were found to be important factors related to microcephaly.

Objective To analyze the status of head circumference development and related factors of microcephaly among infants in Chongqing, China. Methods We included infants aged 7-24 months who had received child health care services at a Chongqing hospital from 1 February 2010 to 1 November 2014. We collected data of sex, age, head circumference, childbirth conditions, mother's pregnancy history, infant feeding patterns, and parents' educational level. Ultimately, 31 902 infants (17 511 males and 14 391 females) were analyzed. The t-test was used to compare head circumference by sex, and logistic regression models used to analyze the related factors of microcephaly. Odds ratios (OR) and 95%confidence intervals (CI) were calculated. Results Among the different age groups (7-8, 9-10, 11-12, 13-18, and 19-24 months), head circumferences for males were (44.3±1.3), (45.2±1.4), (46.1±1.5), (46.8±1.4), and (47.8±1.4) cm, respectively;for females, head circumferences were (43.1±1.3), (44.1±1.3), (44.8±1.4), (45.7±1.4), and (46.7±1.3)cm, respectively. Head circumference for males were all greater than those for girls (t-values were 32.09, 27.73, 32.21, 41.66, and 32.03, respectively; P<0.001). The rate of microcephaly was 3.2%(1 025/31 902) among infants. Birth weights and fetal times were related to microcephaly in infants, with OR (95%CI) values 0.15(0.13-0.18) and 1.31(1.11-1.54), respectively. Factors including urban location, delayed birth, cesarean section, formula feeding, and mixed feeding patterns might reduce the risks of microcephaly compared with suburban location, term birth, eutocia, and breastfeeding; OR (95% CI) values were 0.60 (0.52-0.68), 0.55 (0.44-0.70), 0.76 (0.67-0.87), 0.46 (0.32-0.67), and 0.51 (0.34-0.75), respectively. Conclusion Head circumference development among infants in Chongqing was satisfactory. Family environmental factors, birth conditions, and feeding patterns were found to be important factors related to microcephaly.

Objective To explore the association between lipid profiles and left ventricular hypertrophy in a Chinese general population. Methods We conducted a retrospective observational study to investigate the relationship between lipid markers [including triglycerides, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein (HDL) cholesterol, non-HDL-cholesterol, apolipoprotein A-I, apolipoprotein B, lipoprotein[a], and composite lipid profiles] and left ventricular hypertrophy. A total of 309,400 participants of two populations (one from Beijing and another from nationwide) who underwent physical examinations at different health management centers between 2009 and 2018 in China were included in the cross-sectional study. 7,475 participants who had multiple physical examinations and initially did not have left ventricular hypertrophy constituted a longitudinal cohort to analyze the association between lipid markers and the new-onset of left ventricular hypertrophy. Left ventricular hypertrophy was measured by echocardiography and defined as an end-diastolic thickness of the interventricular septum or left ventricle posterior wall > 11 mm. The Logistic regression model was used in the cross-sectional study. Cox model and Cox model with restricted cubic splines were used in the longitudinal cohort. Results In the cross-sectional study, for participants in the highest tertile of each lipid marker compared to the respective lowest, triglycerides [odds ratio (OR): 1.250, 95％CI: 1.060 to 1.474], HDL-cholesterol (OR: 0.780, 95％CI: 0.662 to 0.918), and lipoprotein(a) (OR: 1.311, 95％CI: 1.115 to 1.541) had an association with left ventricular hypertrophy. In the longitudinal cohort, for participants in the highest tertile of each lipid marker at the baseline compared to the respective lowest, triglycerides [hazard ratio (HR): 3.277, 95％CI: 1.720 to 6.244], HDL-cholesterol (HR: 0.516, 95％CI: 0.283 to 0.940), non-HDL-cholesterol (HR: 2.309, 95％CI: 1.296 to 4.112), apolipoprotein B (HR: 2.244, 95％CI: 1.251 to 4.032) showed an association with new-onset left ventricular hypertrophy. In the Cox model with forward stepwise selection, triglycerides were the only lipid markers entered into the final model. Conclusion Lipids levels, especially triglycerides, are associated with left ventricular hypertrophy. Controlling triglycerides level potentiate to be a strategy in harnessing cardiac remodeling but deserve to be further investigated.

Objective To understand the status and correlative factors on menarche and first spermatorrhea among children and adolescents,in Chongqing,in order to provide theoretical basis for carrying out sex and health education in this population.Methods By random stratified and cluster sampling,10 498 students (5 372 boys and 5 126 girls),5 to 18 years old and living in Chongqing urban districts,were enrolled.General situation and physical features of the population were studied.Statistics analysis system included logistic regression methods,t-test and chi-square test.Results For urban kids,first experience of spermatorrhea was 0.218 years later than those living in the rural areas (Z=-73.287,P＜0.001),but median age for girls in urban areas was 0.073 years earlier than in rural areas (Z=-71.589,P＜0.001).Except for factor as mother' s education level in the family (x2=21.564,P＜0.001),other family or environment related factors did not show significant difference between the two groups of boys (P＞0.05).However,significant difference appeared in average family income (x2=6.175,P=0.046) between two groups of girls.Data from the logistic analysis showed that BMI,hip circumference,height,weight,number of children in the family,time of sleep and the diet structure were associated with menarche.Correlative factors of boys' first spermatorrhea would include:high-energy snacks,hip circumference,weight,height,school type and mother's education level (P＜ 0.05).Conclusion First spermatorrhea and menstruation of boys and girls were closely related to environment of the family,diet and the time of sleep.