1.The effect of bFGF on wound healing of mice lips
Chinese Journal of Pathophysiology 1986;0(02):-
The skin and tunica mucosa of mice lips were cut off by surgical opera-tion. To the local wound of mice lips was applied the basic fibroblast growth factorwhich was produced by gene engineering method. The time to achieve a complete healingtook 4-5 days. The time for control group, bFGF+heparin group and heparin grouptook 9-10 days. These resuits suggested that bFGF had an accelerat healing effect to thelip tissue of mice. Obviously heparin can inhibit the biological activity of basic fibro-blast growth factor.
2.A functional analysis of short stature homeobox (SHOX) gene promoter mutation in idiopathic short stature
Zhiya DONG ; Xiaofei DU ; Wei WANG ; Kenan QIN ; Yuan XIAO ; Defen WANG
Chinese Journal of Endocrinology and Metabolism 2009;25(2):147-149
Objective To investigate the effect of short stature homeobox (SHOX) gene promoter-372G →A mutation on the promoter activity and its mechanism.Methods The luciferase report gene vectors containing human SHOX gene promoter-372G or -372A were contructed.Their transcription activities were detected in chicken chondrocytes.Double-stranded DNA probes containing-372G or-372A were produced by PCR,and used for detecting the affinity with nuclear transcription factors by electrophoretic mobility shift assay(EMSA).Results The transcription activity in a-372A promoter construct was significantly higher than that in the wild type-372G (P<0.01).The result of EMSA showed that-372A gene mutation resulted in loss of the binding affinity to nuclear transcription factors.Conclusion The-372A mutation increases SHOX promoter activity with decreased DNA binding affinity to transcription factors,which may contribute to impaired long bone growth in patients with idio pathic short stature.
3.A HISTOLOGICAL AND HISTOCHEMICAL STUDY OF SOME PRESERVATING METHODS IN LOCAL ISCHEMIC KIDNEY
Yu SUN ; Minhui CHEN ; Weiqian LIU ; Kenan DU ; Bingyan ZHAN ; Yangguang WU ; Linglong WANG ; Rongyang WU ; Ruiqin WAN ; Xiaobin ZHANG
Acta Anatomica Sinica 1957;0(04):-
A comparative observation about histological and histochemical study were made onthe preservating methods of local ischemic kidney of dogs.The methods of this exp-eriment were divided into five groups:A.Local hypothermia;B.Hypothermic perfu-sion solution 500ml,4℃,containing Procaine 450 mg and Heparin 100 mg,infusedinto renal artery;C.Hypothermic perfusion solution 500ml,4℃,containing 20% Ma-nniton 32ml,25% MgSO_4 0.36 ml,Heparin 100 ml,infused into renal artery;D.Localhypothermia and heparin 30 mg injected into the same vessel.E.Local room tem-perature.The specimens were taken from the kidneys of the experimental dogs atvarious period after treated with every method.The results were as follows;A,D and E group presented irreversible histological changes within 4 hoursaftertreatment.Four hours later,the reaction of SDH,ATPase,AlPase werenegative.It suggested that the kidney damage was serious,as well as expressed thatthe effect of these preservating methods for ischemic kidney are not satisfactory.In B group,the histological and histochemical alteration is very light and almostreversible,during 60 days after treatment.In C group,hypothermic perfusionsolution containing Mannitol,MgSO_4 etc either during experiment or follow observa-tion for 60 days,no histological and histochemical alterations were found.It indica-ted that this method is an ideal perservating technique for ischemic kidney.