Objectives To study two airway humidification methods on airway humidification of tracheotomy patients. Methods Sixty patients with traeheotomy were evenly randomized into experiment group and control group:in the former,the method of continuous oxygen atomization was used and in the latter,that of intermittent intratracheal instillation was used.The effects were compared in terms of stimulating cough,sputum clot formation,frequency of suction phlegm,time spent on the airway humidification and incidence of pulmonary infection between the groups.Results The effects in the experiment group were significantly better than in the control group in terms of stimulating cough,sputum clot formation,frequency of suction phlegm,time spent on the airway humidification and incidence of pulmonary infection(all P<0.05).Conclusion Continuous oxygen atomization can improve the effects of airway humidification,reduce the incidence of pulmonary infection and improve the efficiency of nursing work.

Objective To investigate the epidemiological characteristics of patients with craniocerebral injury in primary level hospital of Pearl River Delta and provide evidences for developing reasonable prevention and management policy of this disease. Methods The gender,age,injury time,occupation,injury mechanism, injury information,the relation between the craniocerebral injury and the alcohol drink in the 1 898 patients with craniocerebral injury admitted to Changping People's Hospital of Dongguan in Guangdong Province were analyzed with descriptive epidemiological method. Results There were more male cases with craniocerebral injury than in female ones(1 402 cases vs. 496 cases). The major group of craniocerebral injury consisted of patients aged between 20 to 39 years old〔1088 cases(57.32%)〕. The incidence of the injury in productive workers was higher than that of employees in other kinds of work〔57.48%(1 091 cases)vs. 42.52%(807 cases)〕. The incidences of such cases were the highest between March to May〔28.71%(545 cases)〕and in October〔9.69%(184 cases)〕,December〔11.22%(213 cases)〕than those in other months. The peak days of the incidence in a week were Saturday〔392 cases(20.65%)〕and Sunday〔375 cases(19.77%)〕,but the rate of Thursday was the lowest〔201 cases(10.59%)〕. The peak time in a day was between 18 to 24 o'clock〔961 cases(50.63%)〕. Traffic accident was the leading cause of the injury accounting for 52.95%. The incidence of mild to moderate craniocerebral injury(79.98%)was much higher than that of severe and especially severe injury(20.02%). The alcohol drink was bound up with craniocerebral injury. Conclusion The occurrence of craniocerebral injury has certain regularities concerning the respects of patients' gender, age, occupation,injury time,injury mechanism,types of injury,etc and based on the epidemiological characteristics,a proper management policy should be taken to effectively reduce the morbidity of craniocerebral injury.

Objective To obtain different fragments of human carboxypeptidase H,and evaluate the diagnostic application of the recombination carboxypeptidase H in detecting autoantibody.Methods The coding gene of carboxypeptidase H was ob-tained by RT-PCR.The corresponding prokaryotic expression vectors were constructed and transformed into E.coli to in-duce the expression of the recombination different fragments of carboxypeptidase H.Using these antigen fragments as the coating antigens,the enzyme-linked immunosorbent assay (ELISA)was established for the detection of carboxypeptidase H autoantibody in 95 newly diagnosed type 2 diabetes patients.Results Three fragments of human carboxypeptidase H were obtained,in which the 42~476aa fragment antigen was ideal one.Using the full-length carboxypeptidase H as coating anti-gen,the positive rate of carboxypeptidase H autoantibody was 8.42%.Conclusion Because of the favorable antigenicity,the 42~476aa fragment antigen of carboxypeptidase H could be the candidate antigen for discrimination and diagnosis of latent autoimmune diabetes in adults.

OBJECTIVE: To study the use of mobile phone sports software by the staffs of Centers for Disease Control( CDC)and its impact on their health. METHODS: Using cluster random sampling method,291 staff members of CDC in 7 prefecture-level cities in Hunan Province were selected as research subjects. The self-designed questionnaire was used to investigate the use of mobile phone sports software,and the Sub-Health Scale was used to investigate their health status.The impact of mobile phone sports software on their health was analyzed. RESULTS: The usage rate of mobile phone sports software was 28. 5%(83/291). The utilization rate of mobile phone sports software in postgraduate staffs was higher than that of undergraduates,college graduates and those with less than bachelor's degree( P < 0. 01). The usage rate of mobile exercise software of the CDC staff whose BMI was 18. 5 ~ kg/m~2 was lower than those whose BMI was 24. 0 ~ or 28. 0 ~kg/m~2(P < 0. 001). The sub-health incidence rate in those who used mobile sports software was lower than those who did not( P < 0. 05). The incidences of feeling fatigue,dizziness,lack of attention,upset,more dream,insomnia,poor memory in CDC staffs who used mobile phone sports software were lower than those who did not( P < 0. 05). CONCLUSION: The use of mobile phone sports software has positive effect on health promotion in CDC staffs.

Neuropathic pain is a chronic disease that severely afflicts the life and emotional status of patients, but currently available treatments are often ineffective. Novel therapeutic targets for the alleviation of neuropathic pain are urgently needed. Rhodojaponin VI, a grayanotoxin from Rhododendron molle, showed remarkable antinociceptive efficacy in models of neuropathic pain, but its biotargets and mechanisms are unknown. Given the reversible action of rhodojaponin VI and the narrow range over which its structure can be modified, we perforwmed thermal proteome profiling of the rat dorsal root ganglion to determine the protein target of rhodojaponin VI. N-Ethylmaleimide-sensitive fusion (NSF) was confirmed as the key target of rhodojaponin VI through biological and biophysical experiments. Functional validation showed for the first time that NSF facilitated trafficking of the Cav2.2 channel to induce an increase in Ca²⁺ current intensity, whereas rhodojaponin VI reversed the effects of NSF. In conclusion, rhodojaponin VI represents a unique class of analgesic natural products targeting Cav2.2 channels via NSF.