Objective To study the rehabilitative effect of velvet antler polypeptides-PLGA compound membrane on peripheral nerve injury.Methods Two doses(3 and 15 mg?g~(-1)) of velvet antler polypeptides-PLGA compound membrane(thickness 50 ?m) were used to wrap the sutures of severed sciatic nerves,which were compared with nerve sutures only.2,4 and 6 weeks post-operation,general morphological,electrophysiological,histological and electron microscopic observations and examinations were investigated.Results There were no ulcers in the nars and toes in the treatment group,light conglutination appeared in nerve anastomosis and around tissues.At 6th week post-operation,conglutination was alleviated obviously than 2 weeks post-operation;findings from latency inducing potentials of calf triceps dominated by sciatic nerves showed that recover ratios(%) in treatment group((2 weeks:)9.07?1.44,8.02?1.41;6 weeks: 49.87?9.69,50.11?6.11) were better than those in control group((2 weeks:)2.52?1.83;6 weeks:30.31?6.32) obviously in each time(P

Systematic pharmacological evaluation of material basis for the efficacy of Chinese herbal formula is one of the key issues in Chinese materia medica(CMM),which is in urgent need of solution.However,its scientific connotation is still controversial in academic circles.The material basis for the efficacy of Chinese herbal formula is the total non-endogenous bioactive compound(derived either directly or indirectly from this formula) exerting the therapeutic effects,which is generated by the interaction of the formula with human body and reaches certain concentration in the target organs or tissues.Systematic evaluation of material basis for the efficacy of Chinese herbal formula is the cornerstone of modernization and internationalization of CMM,while the quality control of CMM is its prerequisite.

By digestion with concentration sulfate acid and fuming nitri c acid under refluxing coprecipitation with Fe(OH)3, colorimetry with phenylfluorone-cetyl trimethyl ammonium bromide (CTMAB), germanium (Ge) contents of 18 kinds of Chinese medicinal herbs were determined. The Ge contents in Ganoderma lucidum and oldenladia diffusa were higher than those of others (2.46 and 0.35 ?10-8, respectively).In this method, the recoveries of Ge added to various herbs were in the range of 91%~93%. The detection limit and coefficient variation were 198 ng/g and 0.89%, respectively.

Objective To study the clinical efficacy and safety of rapamycin in combination with CsA and steroid to prevent the acute rejection of kidney transplantation. Methods In an open-label,multi-center study,there were 100 primary renal allograft recipients with cadaveric donors enrolled from 4 transplantation centers in China. The immunosuppressive regimen was the triple therapy of rapamycin in combination with CsA and steriod. Rapamycin was administered in 48 h after grafting. The first dose of rapamycin was 6 mg /day and the maintenance dose was 2 mg /day. Results Eighty-four recipients were followed up for more than 6 months. Rapamycin was discontinued in 16 patients because of the adverse events and other reasons. Eight patients experienced acute rejection and 7 patients were reversed by methyprednisolon therapy. In 6 of the 7 patients,the dose of rapamycin was maintained 2 mg /day. The remaining one was added to 3 mg /day. No recurrence of AR was observed in a continuous follow-up of more than half-year. The most common and significant adverse events were hyperlipoidemia and abnormal liver function.Conclusions The combination of rapamycin with CsA and steroid to treat recipients of kidney transplantation is safe and efficient. There was a low incidence of AR but a high incidence of hyperlipoidemia and abnormal liver function. The rational regulation of the dose may reduce the incidence of the side-effects. Further observation and study are required for long-term application.

Being fundamental preparation for the research of cochlear implants, study of tinnitus's mechanism and establishment of the animal model, the spontaneous discharge of single unit of auditory nerve primary fibers in normal cat was observed and analyzed. They include: Ⅰ, How to identify the spontaneous discharge potential in auditory nerve primary firbers. Ⅱ, The waveform of the spontaneous discharge potential in auditory never, Ⅲ, The discharge rate of spontaneous discharge in auditory nerve primary fibers. Ⅳ, The temporal feature of the spontaneous discharge. Ⅴ, The origin of the spontaneous discharge.

Objective To evaluate the significance of immediate early 1 (IE) mRNA detection by nucleic acid sequence-based amplification (NASBA) in the diagnosis of human cytomegalovirus (HCMV) infection after renal transplantation.Methods The expression of IE-mRNA and pp67-(mRNA) was detected by NASBA in 55 patients after renal transplantation, and pp65 antigenemia (assays) were done for all cases.Results Twenty cases were positive for IE-mRNA. Thirteen cases were suffered from CMV disease with symptoms. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of IE-mRNA by NASBA were 92.3 %, 80.9 %, 60.0 % and 97.1 %, respectively. Time to first IE-mRNA positive detection after transplantation was shortest, compared with pp67 and antigenemia (P

Objective To investigate the clinical features, diagnosis and therapy of avascular necrosis of the femoral head after renal transplantation Methods Of the 24 renal transplantation patients with avascular necrosis of the femoral head 0.7 - 7.8 years postoperatively, 20 cases were unilateral and 4 bilateral respectively. According to the standard of Ficat's, all cases were classed as 0-Ⅳ stages. All cases accepted immunosuppressive therapy that included corticoids. The corticoids were reduced in dose at the time when the patients were diagnosed as having avascular necrosis of femoral head and withdrawn in 2 weeks. Surgical treatment was not done unless the expectant treatment was invalid.Results The clinical manifestations of femoral head avascular necrosis after renal transplantation included knee joint, hip joint, inguina or thigh pain in the earlier period (Ⅰ-Ⅱa stage) and hip joint pain in progression (Ⅱb-Ⅳ stage). Some cases were associated with hip joint flex, adduct, abduction and intorsion dysfunction. After expectant treatment, the symptoms of hip were alleviated or disappeared in 4 cases; In 16 cases, the symptoms of hip were relieved to some extent, but Ficat’s stage had no change. Eight cases had no reaction to expectant treatment and were subjected to surgical operation. In 4 cases, acute rejection occurred after corticoid withdrawal, reversed after therapy by MP and ATG and could tolerance corticoid withdrawal again.Conclusion Corticoid is the main reason of avascular necrosis of the femoral head after renal transplantation. The therapeutic effectiveness of avascular necrosis of the femoral head was not satisfactory and the emphasis was put on the prevention.

6.2 mmol/L) who underwent renal transplantation accepted pravastatin therapy (10 mg, qn) for 8 weeks. The changes of total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C) , triglyceride (TG), endothelin (ET) and nitrous oxide (NO) were measured before and after pravastatin treatment. By using high resolution ultrasound, endotheli-um-dependent relaxing function was measured before and post pravastatin treatment. Thirty people with normal blood cholesterol served as controls and subjected to the above examinations. Results In renal transplantation group, ET was significantly higher and NO significantly lower than in control group. After treatment with pravastatin, NO was significantly increased, while ET, TC, LDL-C and TG were significantly reduced. HDL-C was increased, but no significant difference was found. Flow-mediated vasodilations were greater after pravastatin treatment than before, but smaller than control group. Conclusion Pravastatin can theat dyslipidemia after renal transplantation and improve impaired endothelium-dependent vasodilation.

[Objective]To study the feasibility and significance of a safe arthroscopic entrance technique to posterior compartment of the knee joint.[Method]Exploratory surgery of posterior compartment of 239 knee joints was performed by arthroscope or its working instruments through the combined entrances of anteromedial,anterolateral,intercondylar notch,posteromedial,posterolateral and trans-septal.Their results were evaluated.[Result]With this technique,complete arthroscopic visualization of the posterior compartment of knee joint and arthroscopic procedures for this compartment could be easily performed in 216 cases(239 knees),except 5 cases with arthro-stiffness.There occurred 1 case of saphenous nerve injury but no complications such as injuries of popliteal neurovascular structures.[Conclusion]Combined entrances of posterior compartment of knee joint is a satisfactory approach for arthroscopic surgery of posterior compartment of knee joint and can give a high efficiency and quality of arthroscopic surgery for posterior compartment of knee joint.

Objective To study the effect of delayed cyclosporine(CsA) therapy combined with prophylactic use of antilympho-cyte globulin (ALG) (sequential quadruple immunosuppressive protocol) on early graft function in renal transplantation. Methods The experimental group included 90 renal recipients who were treated with posttransplant quadruple sequential therapy. Their short-term outcomes were compared with those of 215 recipients who were given CsA immediately after transplantation(control group). Results Not only was the recovery of renal allograft function faster in the experimental group ( P 0. 05). Conclusion Delayed CsA therapy in conjunction with prophylactic use of ALG is a desirable protocol for treating posltransplant renal recipients.