Objective The main purpose is to establish a simple method of analytical run length definition through combination of control-based quality control(QC) and average of normals method(AON).Methods Eight test items with different analytical performance were chosen.First,the individualized control-based quality control strategy was designed in the direction of sigma metrics,and the suitable AON rules were selected for each analyte.Then,the selected AON rules were applied to the patient data of successive five workdays.Meanwhile,new individualized control-based QC procedures were also used at 8:00,10:00,12:00 and 14:00 in those days.At last,AON QC result were compared with control-based QC result to define the analytical run for 8 items and the strategy through which laboratory can optimize analytical run length.Results The error detection power of AON algorithms was as good as control-based QC whose performance was excellent.Analytical run length for 8 items involved in this study were defined as follows:triglyceride,potassium,total protein: 8 hours,Chlorine: 6 hours,magnesium:4 hours,calcium,carbon dioxide combining power,sodium: 2 hours.Conclusions For the items with performance above 3.5 sigma,the analytical run was defined mainly depending upon control-based QC,and AON QC was just used to validate control-based result.For the items with performance below 3.5 sigma,the analytical run was defined mainly depending upon AON QC.

Objective To study the change of DNA polymerase beta and XRCC1's expression during malignant celI differentiation.Methods The Eca-109 cells were divided inm 2 groups:differentiation group which cultured with 8-Br-cAMP and control group.The 2 groups cells were cultured 48h simultaneously.The immunocytochemistry was performed to detect the expression of DNA polymerase beta and XRCC1.Results Compared with control group,the expression of DNA polymerase beta and XRCC1 was decreased simultaneously(P＜0.05).Conclusion The differentiation agent can down-regulate the expression of DNA polymerase beta and XRCC1,suggesting that overexpressed DNA polymerase beta and XRCC1 maybe result in mutator phenotype.

Objective: To explore the effect of different selenium sources on the function of humoral immunity and antioxidant capacity of rabbits. Method: Thirty-five rabbits were randomly divided into seven groups and vaccinated with rabbit haemorrhagic disease (RHD) dead vaccine. At the same time, rabbits were injected respectively with sodium selenite (0.1 mg/kg bw and 0.3 mg/kg bw), Kappa-selenocanageenan (0.1 mg/kgbw and 0.3 mg/kg bw), DL-selenomethionine (0.1 mg/kg bw and 0.3 mg/kg bw) and physiological saline as control. Antibody against RHD, activity of GSH-Px and content of MDA in rabbit serum were detected on 0, 10, 20, 30d after inoculation. Results: Sodium selenite (0.1 mg/kg bw), Kappa- selenocanageenan (0.3 mg/kg), and DL-selenomethionine (0.3mg/kg bw) could significantly increase the level of RHD antibody. Sodium selenite (0.3 mg/kg bw) and Kappa-selenocanageenan (0.3mg/kg bw) improved the activity of GSH-Px. All selenium groups could decrease serum MDA, but Kappa-selenocanageenan (0.3 mg/kg bw) showed the best effect. Conclusion: Kappa-selenocanageenan (0.3 mg/kg bw) was better than the lower dosage and other selenium sources in the effects on the function in humoral immunity and antioxidant capacity of rabbits.