BACKGROUND: Glomerular mesangial cell (GMC) is the chief target cell of diabetic nephrosis. A series of functional alternations on mesangial cell (MC) in high glucose condition results in constant development of the disease. Intervention of Chinese herb is very significant in retarding the progression of diabetic nephrosis.OBJECTIVE: To probe into GMC proliferation in high glucose condition and the effects of serum with tangshen tang contained on it.DESIGN: Controlled experiment was designed.SETTING: Laboratory Room of Second Affiliated Hospital to Zhejiang College of Traditional Chinese Medicine.MATERIALS: MCs of SD rats were taken as experimental objects collected from Internal Department of Kidney of Shanghai First People's Hospital in August 2003.Totally 45 SD rats of clean grade were employed from Experimental Animal Center of Zhejiang College of Chinese Medicine, of either sex,weighted with (180±20)g in October 2003 and they were prepared as the animals with herb-contained serum. Tangshen tang [composed of dahuang (Rhubarb), taoren (Semen Persicae), chong (insects), huangqi (Radix Astragali seu Hedysari), digupi (Cortex Lycii Radicis), huanglian (Rhizoma Coptidis),etc.] was concentrated as 3 g/mL after decoction and infiltration at ratio.METHODS: SD rats were randomized as normal group, tangshen tang group and captopril group, 15 rats in each. The serums with tangshen tang and captopril of different doses were applied in MCs. In normal group,gastric perfusion of equal volume of distilled water was applied everyday.Trypan blue staining and MTT method were used respectively to assay the effects on MC toxicity and proliferation after medication.MAIN OUTCOME MEASURES: Activity of GMC in rats after stimulation of medical-contained serums and cellproliferation.liferation was inhibited (P ＜ 0.05 , vs blank group) in constant high-glucose condition and the inhibited proliferation due to high glucose was reversed to various extents after the intervention of tangshentang and captoter the intervention of medical-contained serums on GMCs in rats in every group (cell activity was up to over 94% and P ＞ 0.05, vs blank group).CONCLUSION: High glucose inhibits MC proliferation and tangshentang stimulates MC proliferation.

Objective:To determine the effect of total parenteral nutrition(TPN) supplemented with glutamine(Gln) on the concentration of plasma lipopolysaccharide(LPS)of the multiple organ dysfunction syndrome(MODS) patients.Methods:40 MODS patients in the ICU were randomly divided into routine group(Group A,n=20),the Gln+TPN treatment Group (Group B,n=20).The two groups of patients received treatment of parenteral nutrition with equal nitrogen and equal calorie.Group A were given routine TPN,and Group B were given routine TPN and Gln 0.27 g/(kg?d)[i.e.dipeptiven 0.4 g/(kg?d)] for seven days.The concentration of plasma Gln and LPS were checked before the treatment of TPN and on the first,the third and the seventh day after the treatment of TPN.At the same time 20 healthy blood donators were chosen as the healthy control group(Group C).Results:Before treatment the concentration of plasma Gln of the two patient groups were both lower than that of Group C(P0.05).On the first,the third and the seventh day of the treatment of TPN,the concentration of plasma Gln of Group A was lower than that of Group B(P0.05).After the treatment of Gln plus TPN,the concentration of plasma LPS of Group B was lower than that of Group A(P0.05).Conclusion:The Gln(dipeptiven) can help to decrease the concentration of plasma LPS and the TPN treatment time of the MODS patients.

OBJECTIVE To investigate the antimicrobial resistances of Gram-negative bacilli isolated from patients with lower respiratory infection in intensive care unit(ICU),so as to provide reasonable selections for antibiotic in clinical treatment.METHODS The phlegm specimens from ICU inpatients with lower respiratory tract infection were collected from 2004 to 2007,and drug sensitivity test was performed according to NCCLS standards.RESULTS Gram-negative bacilli were accounted for 75.2% of total strains.The most predominant pathogens were Pseudomonas aeruginosa(42.7%),Acinetobacter baumannii(16.3%),Sternotrophomonas maltophilia(10.4%),Escherichia coli(10.0%),and Klebsiella pneumoniae(6.7%).The prevalence of ESBLs in E.coli and K.pneumoniae increased from 54.5% and 37.5 % in 2004 to 65.6 % and 52.7% in 2007.ESBLs non-producing strains showed lower resistance rates to the aminoglycosides,quinolones and the third and fourth generation cephalosporin than those of ESBLs producing strains.Nonfermentative Gram-negative bacilli were very highly resistant to most antimicrobial agents.CONCLUSIONS The drug resistance rates of Gram-negative bacilli of lower respiratory tract are serious.It is important to investigate the antimicrobial resistances of pathogenic bacteria and rationally use of antibiotics.

Objective To observe the effect of Boari bladder muscle flap surgery in nephron sparing surgery for patients with middle and lower ureteral carcinoma. Methods Totally 80 patients with middle and lower ureteral carcinoma underwent surgery in our hospital from March 2009 to March 2012 were selected and divided into the control group and the observation group,with 40 cases in each group. Patients of the control group were treated with ureteral carcinoma radical prostatectomy while patients of the observation group were treated with Boari bladder muscle flap surgery,and all of them were followed up for 3 years after operation. Observed the renal function,renal tumor markers and the level of inflammatory symptoms of the 2 groups before the operation and 3 months after the operation,and compared the long-term progno-sis of the 2 groups. Results There was no significant difference between the 2 groups in renal function index before operation. The level of BUN,Scr,blood urea and serum uric acid index in the 2 groups were all increased compared with those before operation,but the increase of the control group was significantly higher with statistically significant differences (P<0. 05). The tumor markers CA19-9, Survivin and in-flammatory marks WBC,NEC%,IL-6,hs-CRP had no significant difference before operation,and all the level of the 2 groups were decreased after operation,but there was still no difference between the 2 groups (P>0. 05). The survival rate 1 year,2 years and 3 years after operation also had no significant difference between the 2 groups (P>0. 05). Conclusion The use of Boari bladder muscle flap surgery in nephron sparing surgery for patients with middle and lower ureteral carcinoma can effectively maintain the renal function of patients without affecting the radical and long-term prognosis.

Objectives: To investigate the distribution of common pathogens and their antibiotic resistance from patiens with catheter related sepsis (CRS).Methods: Catheter bacteria cultrure and antibiotic sensitivity test were performed from 69 patiens with CRS.Results: The common pathogens in CRS were fungi (41.1%),Gram-positive cocci (35.6%)and Gram-negtive bacilli (23.3%). Non-C. albicans species were major pathogen (19/30 stranins).The most strains were staphylococcus epidermidis in Gram-positive cocci and the most of them were Methicillin resistant.No vancomycin resistant strains were found. The Gram negative bacilli were often resistant to third generation cephalosporens.Conclusions: The dorminant pathogens of CRS are fungi and gram positive cocci and we should pay more attention to pathogens of resistence to antibiotics. In order to control CRS, CVC must be used reasonably and shorten the duration of retention.

Objective To explore the predisposing factors in the development of acute respiratory failure after abdominal surgery and the factors affecting the therapeutic effect of mechanical ventilation. Methods A (retrospective) study was undertaken for acute respiratory failure after abdominal surgery in 91 patients. The (underline) diseases, introducing causes and efficacy of mechanical ventilation were retrospectively analysed. (Results) Postoperative pneumonia was the cause of acute respiratory failure in 53 cases and ARDS caused by severe abdominal infection and severe acute pancreatitis in 38 cases. Of the 91 cases, complicated with COPD in 38 cases, severe malnutrion 32 cases, and hypokalemia 14 cases. Respiratory failure occurred at(4.08?2.45)days after operation. The duration of mechanical ventilation was(21.66?21.42)days; 33 cases died, and 58 cases were successfully recovered with mechanical ventilation.Conclusions The (management) of acute respiratory failure after abdominal asurgery should be rational use of mechanical (ventilation), adjustment of weaning strategy and avoidance of dependance on mechanical ventilation. Timely treatment of the primary disease, effective control of abdominal infection and aggressive symptomatic and (supportive) treatment are factors that affect the success or failure of mechanical ventilation.

OBJECTIVETo study the biocompatibility of porous calcium phosphate ceramics nanocomposite.

METHODSThe biocompatibility was evaluated via experiments including the hemolysis test, hemopexis test, acute systemic toxicity test, pyrogen test, and intramuscular implant test, in which biphasic calcium phosphate nanocomposite (NanoBCP) presented as leaching solution, suspension or blocks of 5 mmx5 mmxl mm. Animals including New Zealand Rabbits, Kunming mice, SD rats were selected as the host.

RESULTSThe hemolysis of NanoBCP was 1.1% (<5%). Four coagulation index levels were within the normal range. In pyrogen test, the temperature of each experimental rat increased by 0.35, 0.40, 0.28 degrees C (<0.60 degrees C, in accordance with the pyrogen-free criterion for biomedical materials). No consequent death, dyspnoea, organ dysfunction, severe peritoneal irritation or ptosis was observed in acute systemic toxic test. Newly-formed fibrous tissue could be found after the implantation.

CONCLUSIONThe material possesses outstanding biocompatibility and degradability with no toxicity or irritation, contains no pyrogen, as well as better degradation properties than biphasic calcium phosphate.

Animals ; Biocompatible Materials ; Calcium Phosphates ; Ceramics ; Hydroxyapatites ; Mice ; Nanocomposites ; Prostheses and Implants ; Rabbits ; Rats ; Rats, Sprague-Dawley

OBJECTIVETo investigate the relationship between the stages of calcification of various teeth and cervical vertebral bone ages among children and adolescents in Chengdu.

METHODSThe study subjects consist of 256 children and adolescents in Chengdu with age ranging from 10 to 16 years. All panoramic radiographs and cephalometric radiographs were obtained. The relationship with the stages of chronological ages, calcification of various teeth and cervical vertebral bone ages were analyzed.

RESULTSThe Spearman rank correlation coefficient revealed highly significant relationships between cervical vertebral bone ages and chronological ages (r = 0.726, P < 0.01). Dental ages and chronological ages had medium relationship (r = 0.629, P < 0.01), also dental ages and cervical vertebral bone ages medium relationship (r = 0.668, P < 0.01).

CONCLUSIONCervical vertebral bone ages, chronological ages and dental calcification stages have correlation among children and adolescents in Chengdu. The cervical vertebral bone ages have high connection with chronological ages. Cervical vertebral bone age might be a better indicator in judging growth and development of children and adolescents during orthodontic treatment.

Adolescent ; Age Determination by Skeleton ; Cephalometry ; Child ; Female ; Humans ; Male ; Radiography, Panoramic ; Tooth

Objective　To determine the prevalence of human herpesvirus 8 (HHV-8) DNA in acute leukemia (AL) patients. Methods　The presence of HHV-8 DNA sequences in peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) from 50 AL patients was examined using polymerase chain reaction (PCR). Nine human hematopoietic cell lines and PBMC from 30 normal donors were also included. Results　HHV-8 DNA sequences were detected in one case of acute myelogenous leukemia (AML). The specimens from the bone marrow aspirate, peripheral blood and serum of this patient were all positive. None of the normal donors and human hematopoietic cell lines showed evidence of HHV-8 DNA. Conclusion　The results suggest that the prevalence of HHV-8 is low in AL in China.

Objective To investigate the effect of humidex combined with mean temperature and relative humidity on the incidence of bacillary dysentery in Hefei. Methods Daily counts of bacillary dysentery cases and weather data in Hefei were collected from January 1, 2006 to December 31, 2013. Then, the humidex was calculated from temperature and relative humidity. A Poisson generalized linear regression combined with distributed lag non-linear model was applied to analyze the relationship between humidex and the incidence of bacillary dysentery, after adjusting for long-term and seasonal trends, day of week and other weather confounders. Stratified analyses by gender, age and address were also conducted. Results The risk of bacillary dysentery increased with the rise of humidex. The adverse effect of high humidex (90 percentile of humidex) appeared in 2- days lag and it was the largest at 4-days lag (RR=1.063, 95%CI:1.037-1.090). Subgroup analyses indicated that all groups were affected by high humidex at lag 2-5 days. Conclusion High humidex could significantly increase the risk of bacillary dysentery, and the lagged effects were observed.