Drug pairs,an important ingredient in prescription compatibility,is the bridge between Chinese materia medica and prescriptions.This paper discusses the effect of drug pairs in treating diabetes and its complications,thus,studys the effective prescriptions and methods of treating diabetes and its complications.

The main pathogenesis of diabetes is that spleen weakness losing transports induces nutrient substance metabolic disturbance in the body,so as results the patient's GLU elevated.Spleen weakness is the foundation of diabetes and its complications.In the therapy it should be treated from the spleen using activating spleen-energy method.In this article it proposed that in clinic we could apply the five methods in terms of dissipating dampness,dispersing the depressed liver-energy,promoting blood flow,eliminating phlegm and warm the kidney to activate spleen-energy in treating diabetes and its complications.

Objective:To discuss the influence of Didang Decoction on glomerulosclerosis.Methods:The glomerulosclerosis model was established by uninephrectom and injection of adriamyci,and was treated by modif ied Didang Decoction and taken western medicine Losartan as the comparison group.To observe 24-hour urinary protein and kidney function of model group and each treatment group.Reverse transcriptase Polymerase Chain Reaction(RT-PCR) was used to determine the expression of TIMP-1 and PAI-1 in each group of rats kidney tissue.Results:Didang Decoction could decline elevated 24-hour urinary protein and improve kidney function,and decline the expression of TIMP-1 and PAI-1mRNA in glomerulosclerosis rats.Conclusion:Didang decoction could delay renal failure,it may be related to the down-regulated expression of TIMP-1 and PAI-1mRNA.

Three methods for isolation of nitrite oxidizers-dilution method, silica-gel plate method and enrichment culture-capillary pipette method were compared. Among them the dilution method was most feasible and efficient.