Objective To study the effects of different concentrations of antisense oligodeoxynucleotides (ASODN) on human pancreatic cancer cell line PaTu8988s. Methods Human pancreatic cancer cell line PaTu8988s in exponent growth stage are adopted. We observe the effect of different time point on the PaTu8988s cell at 12、24、48 and 72 hour. Results The inhibitory rate on PaTu8988s cell line is 42.25%、66.29%、69.55%、74.58% and 66.20%、91.43%、98.18%、98.33% for ASODN concentrations of 50 ?g/ml and 100 ?g/ml at 12、24、48 and 72 hour, respectively. Conclusion The inhibitory effect of ASODN began from 12 hour and becomes more obvious at 48～72 hour. The higher the concentration of ASODN, the earlier the peak of inhibited rate.

[Objective]To explore Professor LU Keda's clinical experience in treating IgA nephropathy with Yiqi Yangyin Qufeng prescription.[Methods]Through outpatient learning with teacher,consulting IgA nephropathy related literature,Professor LU's clinical experience in treating IgA nephropathy with Yiqi Yangyin Qufeng Prescription was summarized,and one medical case was provided to support the treatment.[Results]Professor LU points out that the early IgA nephropathy patients are deficient in spleen and kidney,and always invaded by wind easily.He applies the thought of syndrome differentiation and prescription called"state target binding"from Academician TONG Xiaolin,taking"Qi and Yin deficiency syndrome"as the state,and the physical and chemical indexes"proteinuria and hematuria"as the target,using Yiqi Yangyin Qufeng Prescription for treatment.The medical case mentioned later was identified as early-stage IgA nephropathy,belonged to Qi and Yin deficiency syndrome.The treatment was to invigorate the spleen and tonify Qi,nourish Yin and promote body fluid and dispel wind.It had great curative effect by using Yiqi Yangyin Qufeng Prescription.[Conclusion]Professor LU's experience in establishing the treatment of IgA nephropathy from the perspective of"state target syndrome differentiation"is worth learning and inheriting.

OBJECTIVETo investigate the effects of activation of the GLP-1 receptor on the p38MAPK signaling pathway in hepatic stellate cells (HSCs).

METHODSHSCs were isolated and identified according to morphological features; the levels of GLP-1R protein were determined by western blotting.The HSCs were randomly divided into a control grouP (normal saline treatment) and experimental grouP(liraglutide treatment); after 120 hours, the expression of p38MAPK mRNA was examined by RT-PCR and of phosphorylated (p)-p38MAPK protein was detected by western blotting.

RESULTSGLP-1R proteins were detected in the HSCs. Compared with the control group, the experimental group showed significantly decreased p38MAPK mRNA and p-p38MAPK protein (both P < 0.01).

CONCLUSIONThe p38MAPK signaling pathway could be down-regulated when GLP-1R is activated in HSCs.

Cells, Cultured ; Glucagon-Like Peptide 1 ; analogs & derivatives ; pharmacology ; Glucagon-Like Peptide-1 Receptor ; Hepatic Stellate Cells ; metabolism ; Humans ; Liraglutide ; MAP Kinase Signaling System ; RNA, Messenger ; Receptors, Glucagon ; metabolism ; p38 Mitogen-Activated Protein Kinases ; metabolism

Objective To exploring the distribution characteristics of renal disease pathology spectrum in Zhejiang Provincial Hospital of Traditional Chinese Medicine.Methods The pathological data of 602 patients who underwent renal biopsy at Zhejiang Provincial Hospital of Traditional Chinese Medicine from January 2016 to June 2021 were collected and retrospectively analyzed.Results ①Among the 602 patients,there were 319 males and 283 females,with a male to female ratio of 1.13∶1.The patient's age was 9-78 years old,average(44.44±15.22)years old.There are statistical differences in the gender ratio and age distribution of various pathological types.②There were 471 cases of primary glomerular disease(PGD),117 cases of secondary glomerular disease(SGD),5 cases of tubulointerstitial nephritis,and 9 cases of hereditary kidney disease.③The most common PGD is immunoglobulin(Ig)nephropathy(242 cases),followed by membranous nephropathy(110 cases).④The most common SGD was diabetes nephropathy(42 cases),followed by lupus nephritis(22 cases).Conclusion PGD is the most common pathological type of renal biopsy,with IgA nephropathy and membranous nephropathy being the most common.Diabetes nephropathy is the most common SGD.In recent years,the incidence rate of diabetes nephropathy has increased significantly.