1.Studies on Operative Treatment of Huge Rhe gmatogenous Retinal Detachment
Journal of Third Military Medical University 1983;0(04):-
Twelve patients with huge rhegmatogenous retinal detachment were treated with electrocoagulation and/or cryosurgery supplemented with extrascleral buckling, annular knotting or macular sling technique. 11 cases were cured but failure occurred to one case. After treatment, the vision was improved in various degrees in different patients.It is concluded that even though the rhegma is large and the retina is wholly detached, the cure rate can be increased if careful preoperative examination and preparation is made, appropriate operative measures selected, electrocoagulation and cryosurgery reasonably adjusted, and the rhegma securely buckled.
5.Blue light-induced replicative senescence of rat retinal pigment epithelial cells
Yifeng KE ; Song CHEN ; Rui HAO
Chinese Journal of Ocular Fundus Diseases 2009;25(6):462-465
Objective To investigate the relationship between exposure intensity and illumination time of blue light and replicative senescence of rat retinal pigment epithelial (RPE) ceils.Methods Thirty-six 12-14 weeks Wistar rats were kept in the cage with a blue-light bulb [(450±10) nm],and were randomly divided into four groups (no light,nature light,500 lx light and 1000 lx light illumination),each has nine rats.The rats in each group were further divided into three subgroups according to illumination time (one month,two months or three months).Eyeballs were collected after intraperitoneal injection of 10% chloral hydrate.The right eye of each rat was embedded in paraffin and sectioned for hematoxylin-eosin (HE)staining,while frozen sections of the left eye were stained for the senescence-associated β-galactosidase (SA-β-Gal).The data were analyzed by SPSS11.5 statistical software.Results The amounts of SA-β-Gal positive RPE cells were significantly different between all groups under the same illumination time 17 (P=0.000),and between all subgroups of different illumination time with same exposure intensity (P<0.01)except for the control group (no light).Conclusion Blue-light can induce replicative senescence in rat RPE cells in an intensity and time-dependent manner.
8.Sorting methods of breast cancer stem cells
Chongyong GUO ; Keying SONG ; Ke LI
Journal of International Oncology 2011;38(5):358-361
In recent years, the theory of cancer stem cells has provided a new perspective on the treatment of cancers including breast cancer. The accurate sorting of breast cancer stem cells is critical. The sorting procedure is consist of 4 steps: isolation of side population, serum-free suspension culture,determination of specific cell surface markers and of the activity of aldehyde dehydrogenase 1 ( ALDH1 ) enzymatic through the ALDEFLUOR assay. Some studies choose drug resistance as an additional method for sorting cancer stem cells because of the enrichment of cancer stem cells after chemotherapy . The controversy about the sorting outcome of breast cancer stem cell mainly focus on molecular markers like CD44+ CD24- and ALDH1+ . The problem needed to be settled is to identify which sorting method and markers are appropriate.
9.Early Intervention on Intelligent Development of Premature Infants
Zunyu KE ; Meng MING ; Song LIANG
Chinese Mental Health Journal 1991;0(04):-
Objective:To investigate the effect of early intervention on intelligent development of the premature infants. Method:62 premature infants were randomly assigned to the intervention group and the control group. The early intervention began from the birth of the infants to their 2 years old.Result:At the age of one and two, the mental development index (MDI) and psychomotor development index (PDI) were significantly higher in the intervention group than in the control.Conclusion:Early intervention can improve intelligent development of premature infants.
10.Immune-related gene expression profile in multiple sclerosis with gene chips
Guiqin SONG ; Weiping WU ; Ke ZHU ;
Chinese Journal of Neurology 2001;0(01):-
Objective To construct the immune genes expression profile database of multiple sclerosis (MS) and to screen the high immune related candidate genes by using microarray analysis Methods Polymerase chain reaction (PCR) products on behalf of 484 immune related genes from mixed tissue library were assembled on the modified Oako glass slide The general RNA from MS patients and normal persons lymphocytes was transcripted to cDNA by RT PCR and labelled with cy 3 and cy 5 The two probes were mixed and hybridized with the above mentioned gene chips Scan array 4000 was used for scanning the hybridizing signals and GenePix Pro3 0 for date analysis Results Of the total 484 double genes monitored,22 genes of group one showed changes in expression level of the ratio outside 0 5 and 2.0;27 genes differ in group 2;72 genes change in group 3 With the same consistent gene ID of the double genes, the group one was 6,the group tow was 9 and the group three was 30 Conclusions These results show that the different expression of immune related genes might occur between MS patients and normal persons It might also afford a view of the changes that these genes should be probably related to the occurrence, development or progress of MS The cell immune related genes might be the important and most immune related genes involved in MS pathogenesis The results suggest the deeper insights into the mechanism,relapsing and treatment of MS