This paper proposes a teaching method on opening computer laboratory for students in medical colleges to learn and practice,designs the details of the teaching method on opening computer lab and discusses the managements of opening computer lab in our practice.

Objective:To study the influences of astragulas(黄芪) on cerebroischemiareperf usion injury in gerbils.Methods:The cerebral tissue delayed neuron death(DND) models of gerbil were established by means of ligating bilateral carotid arteries for 15 minutes then reperfusing for 48 hours and divided into shamoperation(SO) group,ischemiareperfusion(IR) group and astragulas injection (AI，黄芪注射液) group.The activities of Na ＋K＋ATPase and nitric oxide synthase(NOS) and the changes in contents of nitric oxide(NO) and excitat ory amino acid(EAA) in cerebral tissue were compared in different groups.Results:(1)In comparison with SO group the activities of Na＋K＋ATPase in IR and AI group were obviously decreased furthermore the decreases in IR group were mo re significant than those in AI group(all P<0.01).(2) The changes in co nten ts of lactic acid(LD) in cerebral tissue in all the groups had not statistically significance.(3)In comparison with SO group the activitis of NOS and the conten ts of NO in IR and AI group were reduced but the reductions in IR group were muc h more significant (P<0.05).(4)The contents of glutamic acid(Glu) in IR and AI group were higher than those in SO group and the increases in IR group w .05),meanwhile they in IR group were higher than those in AI group but the di fference was not significant.(6)Among all the groups the contents of γam inobutyric a cid (γGABA) and glycine(Gly) had not significant differences (all P>0.05).Conclusions:The mechanisms of astragulas on anticerebral ischemiareperfusion inj ury may be related to its preventing the reductions of activities of Na＋K ＋ATPase,NO S,and the decreases in synthetic volume of NO as well as its heightening the con tents of EAA after ischemia of brain tissue.

Objective To investigate the relationship between the levels of serum glial fibrillary acidic protein (GFAP) ,ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) ,CT findings and outcome in patients with severe traumatic brain injury .Methods En-zyme linked immunosorbent assay (ELISA) was conducted to detect the serum level of GFAP and UCH-L1 in 62 patients with se-vere traumatic brain injury at the time of 12 h and 36 h after the trauma .CT scans of the brain were obtained within 12 h of injury . Outcome was assessed by glasgow outcome scale (GOS) at 6th month .The relationship between GFAP ,UCH-1 ,CT findings and outcome were analyzed .56 healthy persons were selected as control group .Results The concentrations of serum GFAP and UCH-L1 of patients were significantly higher than those of the control group (P< 0 .05) ;GFAP levels were higher in patients with mass lesions than in those with diffuse injury while UCH-L1 levels were higher in patients with diffuse injury (P< 0 .05) ;the levels of GFAP and UCH-L1 of patients with unfavourable outcome(GOS 1 - 3 scores) were significantly higher than those of patients with favourable outcome(GOS 4 - 5 scores) ,and the concentrations of biomarkers were significantly negatively correlated with outcome . Conclusion Serum levels of GFAP and UCH-L1 are good predictors for severity and outcome in severe traumatic brain injury (TBI) .The levels of GFAP and UCH-L1 could reflect different injury pathways which were different in patients with mass lesions and diffuse injury remarkbly .GFAP and UCH-L1 could provide better characterization of subjects for specific types of cellular dam -age than that obtained with CT alone .

C-reactive protein(CRP)is the most sensitive indicator of acute phase proteins.A growing body of evidence suggests that atherosclerosis,as a main cause of cerebral infarction,is a chronic inflammtory process.The present studies have found that the CRP levels and genetic polymorphisms are associated with atherosclerosis and cerebral infarction.As compared with coronary artery disease,the association between high-sensitive CRP and ischemic stroke is more closely.This article reviews the advaces in research on the biological characteristics of CRP,the factors influencing CRP levels,the CRP levels and the correlation between genetic polymorphisms and cerebral infarction.

Vascular endothelial growth factor is a vascular endothelial cell-specific mitogen.It is the most important angiogenic factor.VEGF expresses highly after cerebral infarction.It plays importmt roles in angiogenesis and neuroprotection.At the same time,its overexpression will also increase vascular permeability;thereby it may aggravate brain edema.This article reviews the advances in research on VEGF and its receptors and cerebral infarction.

BACKGROUND: Intravascular stent implantation is one of the main methods for intra-extracranial artery stenosis. The safety and efficacy of stent implantation has been elevated, and symptoms or ischemic advert events with artery stenosis has been reduced. However, risks of thrombosis, cerebralhemorrhage, restenosis, and hyperperfusion still existed. OBJECTIVE: To investigate the influence of atorvastatin on ischemic adverse events following cerebral middle artery stent implantation.METHODS: Twenty-four patients received cerebral middle artery stent implantation were randomly divided into 2 groups. In the treatment group, patients were received atorvastatin 40 mg + plavix 75 mg + aspirin enteric-coated tablet 300 mg, once per day; in the control group, patients were received plavix 75 mg + aspirin enteric-coated tablet 300 rag, once per day. Ischemic adverse events, such as in-stent restenosis, transient ischemic attack, cerebral infarction, or re-interventional therapy were observed by transcranial Doppler, digital subtraction angiography, nuclear magnetic resonance imaging, diffusion weighted imaging, blood lipid level and C-reactive protein level examinations prior to and at months 1, 3, 6 and 12 after stent implantation.RESULTS AND CONCLUSION: Compared with the control group, the levels of blood lipid and C-reactive protein were obviously decreased at months 1, 3, 6 and 12 after treatment (P<0.05, P<0.01 ), with dramatically declined ischemic adverse events (P<0.05, P<0.01 ). Compared with before operation, the levels of blood lipid and C-reactive protein were decreased after operation in the treatment group (P<0.05, P<0.01). No server adverse events occurred in patients treated by atorvastatin, which showed 40 mg was safe for patients. The results revealed that atorvastatin combined with antiplatelet therapy can prevent ischemic adverse events following cerebral middle artery stent implantation.

Objective; To explore the possible effect of hyperthermia combined with Prog on a human tongue cancer cell line Tca8113 and drug-resistance cell line Tca8113/BLM in vitro. Methods;The effect of hyperthermia combined with Prog on Tca8113 and Tca8113/ BLM cells were determined by MTT. The concentration of ADM, the expression of P-gp and MRP were detected by flow cytometry (FCM). Results;Compared with Prog or hyperthermia, the hyperthermia at 41 X. for 1 h combined with Prog showed obvious inhibitory effect in Tca8113 and Tca8113/BLM cells(P<0.01). The concentration of ADM in Tca8113 and Tca8113/BLM cells increased obvi-ously(P<0.01). Expression levels of P-gp and MRP in Tca8113 and Tca8113/BLM cells decreased significantly. Conclusion:The hyperthermia combined with Prog can increase the intracellular concentration of ADM in Tca8113 and Tca8113 /BLM cells, which reverse the drug-resistance of Tca8113/BLM cells to BLM and down-regulate expressions of P-gp and MRP.

Objective To explore the relationship between the level of high-sensitivity C-reactive protein (hs-CRP),CRP 1059G/C gene polymorphism and cerebral infarction(CI).Methods The CRP 1059G/C genotype and allele frequencies were assayed by polymerase chain reaction-restricted fragments length polymorphism (PCR-RFLP) in 105 patients with CI and 121 controls.The level of serum hs-CRP was detected by immune-turbidimetry.The relationship between the condition of CI patients,the level of serum hs-CRP and CRP 1059G/C genotype and allele frequencies were anaysed.Results Compared to the control group,the CRP 1059G/G genotype and G allele frequencies in CI group were statistically higher,G/C+C/C genotypes and C allele frequencies were statistically lower(all P

Objective To investigate mother-to-child transmission of HIV-1 and the factors that affect transmission. Methods 136 children who were born to HIV positive mothers in a high HIV prevalence region were interviewed and blood samples were examined for anti-HIV anti-HGV and HIV RNA or HIV DNA. Results Among 136 children who were born to HIV positive mothers, 50 were infected with HIV. The MTCT rate was 36.8% (50/136). Thirty eight DNA fragments of HIV-1 gag gene P17 obtained from HIV-1 seropositive mothers were amplified by PCR from uncultured peripheral blood mononuclear cells (PBMCs). Sequence analyses showed that all of them were HIV-1 B subtype. The HIV vertical transmission rate among mothers with AIDS (67.4%, 31/46) was significantly greater than among those with HIV alone (21.1%, 19/90), P