Objective:To investigate the effect of pcDNA3.1 vector on house keeping gene expression and set guide to further study work in using pcDNA3.1. Methods:5 cell lines were transfected with pcDNA3.1,48 h post transfection total RNA was extracted and applied for real time PCR to detect the RNA level of GAPDH、 ?-actin、18sRNA difference.Meanwhile,extract total protein and analyze the GAPDH and?-actin expression by Western blot.Results:RNA and protein levels all show of a decrease in 5 cell lines after transfection,with the most obviously ?-actin.Conclusion:pcDNA3.1 might have non specifinhibition effect to cell,when using pcDNA3. 1 for gene function research,it is not recommend to use ?- actin as reference gene.

Angina, Unstable ; diagnostic imaging ; therapy ; Angioplasty, Balloon, Coronary ; Coronary Angiography ; Humans ; Myocardial Infarction ; diagnostic imaging ; therapy ; Randomized Controlled Trials as Topic

Health Services ; Humans ; Medicine, Chinese Traditional ; Professional Practice

Cardiovascular Diseases ; Cardiovascular System ; Diet, Vegetarian ; Humans ; Integrative Medicine ; Vegetarians

Humans ; Hypertension ; therapy ; Integrative Medicine ; Medicine, Chinese Traditional

Drugs, Chinese Herbal ; Education, Medical ; Hematologic Diseases ; therapy ; Humans ; Medicine, Chinese Traditional ; Wills

China ; Humans ; Integrative Medicine ; trends

Chronic Disease ; drug therapy ; prevention & control ; Humans ; Longevity ; Medicine, Chinese Traditional

Critical Pathways ; Integrative Medicine

Esophageal Neoplasms ; mortality ; surgery ; Humans ; Quality of Life ; Survival Analysis