Objective:To investigate the effect of pcDNA3.1 vector on house keeping gene expression and set guide to further study work in using pcDNA3.1. Methods:5 cell lines were transfected with pcDNA3.1,48 h post transfection total RNA was extracted and applied for real time PCR to detect the RNA level of GAPDH、 ?-actin、18sRNA difference.Meanwhile,extract total protein and analyze the GAPDH and?-actin expression by Western blot.Results:RNA and protein levels all show of a decrease in 5 cell lines after transfection,with the most obviously ?-actin.Conclusion:pcDNA3.1 might have non specifinhibition effect to cell,when using pcDNA3. 1 for gene function research,it is not recommend to use ?- actin as reference gene.

Chronic Disease ; drug therapy ; prevention & control ; Humans ; Longevity ; Medicine, Chinese Traditional

Drugs, Chinese Herbal ; Education, Medical ; Hematologic Diseases ; therapy ; Humans ; Medicine, Chinese Traditional ; Wills

China ; Humans ; Integrative Medicine ; trends

Drugs, Chinese Herbal ; administration & dosage ; standards ; Humans ; Injections, Intravenous ; Pharmaceutical Preparations ; administration & dosage ; standards ; Quality Control

Achievement ; Integrative Medicine ; Periodicals as Topic

ObjectiveTo investigate the risk factors for airway management after tracheal extubation in old patients. Methods280 patients underwent general anesthesia were enrolled in the study. The procedure of anesthesia induction and maintenance were in the routine method. The patients were with tracheal extubation under clinical standards after operation. Dyspnea was recorded after tracheal extubation. They were divided into dyspnea group and general group. Twelve perioperative variables, ten variables in operation and six post-operative variables of two groups were compared respectively. ResultsThe incidence of dyspnea after tracheal extubation was 8.6％. Analysis identified that obesity( BMI ≥25kg/m2 ), preoperative lung disease and without postoperative neostigmine were the significant risk factors for dyspnea. ConclusionBMI≥25kg/m2 ,preoperative lung disease and without antagonist muscular relaxant were the main risk factors for dyspnea after tracheal extubation in old patients.

The morbidity and mortality of intracerebral hemorrhage (ICH) are very high. Brain injury involves in several mechanisms after ICH, including the direct tissue destruction by the mechanical force on brain tissue surrounding the hernatoma in the process of hematoma formation, the release of clot-derived factors (thrombin, hemoglobin degradation products), inflammatory response and complement cascade reaction, etc. This article reviews the above mechanisms

Blood Circulation ; drug effects ; Cardiovascular Agents ; therapeutic use ; Cardiovascular Diseases ; prevention & control ; China ; Humans ; Medicine, Chinese Traditional

Academies and Institutes ; organization & administration ; China ; Combined Modality Therapy ; methods ; trends ; Drugs, Chinese Herbal ; therapeutic use ; Education, Medical, Continuing ; Humans ; Integrative Medicine ; legislation & jurisprudence ; trends ; International Cooperation ; Medicine, Chinese Traditional ; methods ; trends ; Western World