Objective To investigate the feasibility and timing of laparoscopic cholecystectomy(LC) for the treatment of acute pancreatitis accompanying gallstones. Methods A retrospective analysis was made on clinical data of 21 patients with acute pancreatitis accompanying gallstones treated by LC between January 1999 and August 2003 in this hospital. Results The LC was performed 20~40 days after the onset of pancreatitis and 15 days after the recovery of serum and urinary amylase levels.An abdominal drainage tube was placed in 9 patients.Postoperative recovery was uneventful in 19 patients,who got out of bed on the surgery day or on the first postoperative day and began to take food 12 hours after operation,with the abdominal drainage tube removed 24~72 hours postoperatively and no complications encountered.Recurrence of acute pancreatitis happened in 2 patients on the 3 postoperative days and was relieved from symptoms with conservative treatment.The duration of postoperative hospital stay was 4~20 days(mean,7 days).Follow-up checkups in the 21 patients for 6 months ~ 5 years(mean,19 months) found no recurrence. Conclusions Laparoscopic cholecystectomy is feasible and safe for patients with acute pancreatitis accompanying gallstones in the period of 20~40 days after the onset of pancreatitis.Careful patient selection,proper peri-operative treatment,strict adherence to techniques,and use of postoperative drainage are considered essential.

Objective: To study the protective mechanism of Donepezil on apoptosis of PC12 cells induced by amyloid beta protein. Methods: PC12 cells were cultured as previously,then amyloid beta protein and Donepezil were used to treat PC12 cells.Morphology observation,TUNEL and immunocytochemistry were employed to investigate the damage of PC12 cells induced by A?,protection of Donepezil on apoptosis of PC12 cells and the expression of Bcl2 and Caspase-3 in A?-treated group and Donepezil-treated group. Results: A? induced apoptosis of PC12 cells in a dose-dependent way;Compared to the group treated with A? only and the group post-treated with donepezil, the apoptosis index of PC12 cells(17.29?0.83)% and expression of Caspase-3(26.46?2.87)% were significantly downregulated in the group pretreated with donepezil(P0.05). Conclusion: This study demonstrated that Donepezil can protect PC12 cells against apoptosis induced with amyloid protein,which was closely related to the time of interference.The mechanism of protection was involved with the expression of Bcl-2 and Caspase-3.

Anticoagulants are recommended for the prevention and treatment of a wide variety of thromboembolic events.Although existing anticoagulants are effective,their usage is limited by parenteral administration or the requirement for frequent monitoring and subsequent dose adjustment.Therefore,there is an urgent need for novel,oral agents with a predictable anticoagulant action.Because of its key position in the coagulation cascade and its limited roles outside of coagulation,Factor Xa has presented as an attractive target for novel anticoagulants.As a result,the past decade has witnessed an explosion of research into small-molecule,oral,direct Factor Xa inhibitors,and some are now in clinical development.Rivaroxaban is currently furthest ahead in its developmental program,having entered phase III in 3 indications.It is hoped that,before long,these anticoagulants will allow us to enter an era of convenient,oral anticoagulation,without the need for regular monitoring or dose adjustment.

Transjugular intrahepatic portosystemic stent shunt(TIPSS)was per- formed in 30 cases of liver cirrhosis with portal hypertention.Operations were susceeded in 29 cases with only one failure.Complications happened in 7 cases,In 29 patients the average blood bilirubin was 25.12?9.80 mmol/l before TIPSS turning to 50.46?34.50 mmol/l after TIPSS;The average blood amonia was 152.33?65.30 ?g/dl before TIPSS changing to 233.33?599.96 ?g/dl after TIPSS.The contributing factors were due to liver function state and shunting channel diameter for causing hepatic encephalopathy and jaundice.Other complications such as multipule liver abscesses,pneumonia,bacteriemia were related to the accompanied diseases and operative technique.The authors introduced the principles of pre- vention and treatment of complications,together with the indications and contraindications of TIPSS.

Heterogeneous-nuclear ribonucleoproteins A1 (hnRNP A1) is one of the important members of hnRNPs family. The function of hnRNP A1 is closely related to RNA transcription, mRNA translation, splicing of splicing sites, splicing site selection, pre-RNA maturation and degradation, cell proliferation and transformation. Overexpression of hnRNP A1 in various types of digestive system tumors is associated with poor prognosis and may serve as an early biomarker for cancer. This article reviews the recent advances in the development of hnRNP A1 and characterizes their roles in cellular and gene expression and in the development of human tumors, highlighting that hnRNP A1 is likely to be an important indicator for tumor prognosis and a potential drug target for the treatment.

Objective To study the natural history of HIV infection among paid blood donors(PBD).Methods Two thousand three hundred and sixty four individuals infected with HIV/AIDS via blood donation in twelve villages of Henan province were included in this study,Retrospective data were analyzed in combination with a cross-sectional survey.Results The number of deaths were 709 among these 2 364 subjects under study by the end of 2004,with a mortality rate of 30.1%.Among 712 deaths,32.8% were companied with serious lung infections,18.1% were complicated with serious diarrhea,8.2%-with central nervous infections,8.2%-with cirrhosis,6.6%-with various types of cancers,3% died of suicide and 23% died with unclear cause.Of them 544 AIDS patients died before the ARV treatment was available in rural areas of Henan at the end of 2002,with a mortality rate of 23.0%.The median survival period for individuals with HIV infection was 9.5 years,and the median incubation period was about 8.5 years.Conclusion The median aurvival period for individuals with HIV infection via blood donation in rural areas of Henan was 9.5 years and the median incubation period was about 8.5 years among them.

Objective:To adopt the brain lesion model of mouse induced by overload aluminum to study the relationship between monoamine oxidase B disorder and neurodegeneration induced by overload aluminum(Al).Method:Aluminum overload models were established by injection of 3?l AlCl3 into lateral ventricle of miceeach day for 5d.The AlCl3 solutions injected in different modelswere with different concentrations and they were 0.125%,0.25%,0.5%.On d10,d20,and d30 after the final administration of AlCl3 solution,the learning and memory function of mice,pathomorphology of hippocampi,and MAO-B activity were determined.Results:Aluminum overload elevated MAO-B activity,caused karyopyknosis and loss of neurons in CA1 area of hippocampi,in dose-and time-dependent manners.Conclusion:The results indicate that neurodegeneration induced by Al toxicity may be related to the monoamine oxidase B homeostasis interfered by Al overload.

Objective: To explore influence of rehabilitation intervention on quality of life in patients with coronary heart disease (CHD). Methods: A total of 108 CHD patients were selected from department of cardiology of our hospital. They were randomly and equally divided into routine treatment group and rehabilitation intervention group (received bedside rehabilitation intervention based on routine treatment). The course of treatment was eight weeks. The medical outcomes 36 item short form health survey (SF-36) was used to assess quality of life before and after treatment of two groups. Results: There were no significant difference in scores of SF-36 at primary evaluation between two groups, P>0.05; when re-evaluation after eight-week treatment, there were no significant difference in all scores of SF-36 in routine treatment group compared with before treatment, P>0.05. Compared with primary evaluation and routine treatment group after treatment, there were significant improvements in all indexes [Physiology: bodily pain (69.89±16.84) vs. (54.62±16.77) vs. (56.63±6.74), role-physical (54.56±35.62) vs. (29.84±39.07) vs. (29.44±37.12), general health (65.74±15.82) vs. (48.49±8.79) vs. (48.28±10.29),Psychology: vitality (62.92±14.31) vs. (37.57±16.65) vs. (38.31±16.32), role-emotional (51.37±18.94) vs. (25.05±14.57) vs. (28.27±12.04), social functioning (74.02±9.35) vs. (47.49±9.87) vs. (51.35±9.29), mental health (72.18±13.26) vs. (55.69±14.05) vs. (58.43±11.36), P<0.01 all] except physical functioning in rehabilitation intervention group after treatment. Conclusion: Rehabilitation intervention can effectively improve quality of life in patients with coronary heart disease.

Objective To observe the clinical therapeutic effect of combinational therapy (Warming Needle Moxibustion &Tuina) on patients with knee joint osteoarthritis .Methods 72 cases with definite diagnosis of knee joint osteoarthritis were ran-domlydividedintotreatmentgroup(receivedWarmingNeedleMoxibustion & Tuina)orcontrolgroup(receivedTuina)with36pa-tients in each group .The pain degree and motor function of knee joint were assessed before and after 4-courses treatment .Results The overall effect rate in treatment group was 94 .45% ,while it was 83 .33% in control group ,the difference was statistically signif-icant(P<0 .05) .VAS score and Lysholm knee scoring scale indicated that significant difference was detected in both groups after treatment(P<0 .05) ,the difference between groups was significant measured by Lysholm knee scoring scale and VAS score (P<0 .05) .Conclusion Warming Needle Moxibustion & Tuina can relieve joint pain and improve motor function of patients with knee joint osteoarthritis effectively .

P-glycoprotein(P-gp)and CD44 have been characterized and are known to be determinants of multidrug resistance(MDR)and cancer metastases.Recent researches suggest that there is a correlation between P-glycoprotein(MDR)and CD44(invasion and metastases).A better understanding of any relationship between drug resistance and tumor metastasis may lead to more effective tumor treatment.