1.Anti-human Cytomegalovirus Activity of Hot Water Extract from Sasa senanensis Rehder
Rie YAMADA ; Kazuhiko AKUZAWA ; Changxiao BI ; Zhuan LI ; Hidetaka SADANARI ; Keiko MATSUBARA ; Tsugiya MURAYAMA
Japanese Journal of Complementary and Alternative Medicine 2009;6(1):17-25
Objective: Anti-Human cytomegalovirus (HCMV) activity of Kumazasa (Sasa senanensis Rehder) extract were investigated in the human embryonic lung (HEL) fibroblast cell.
Method: This study examined the effect of hot water extract from Sasa senanensis Rehder on HCMV replication, viral-specific major immediate early (IE) gene expression, and protein synthesis.
Results: Treatment of HCMV-infected HEL cells with that Sasa senanensis Rehder extract inhibited the cytopathic effect and viral production in a dose-dependent manner. Further, real-time RT-PCR assay and Western blotting analysis revealed that Sasa senanensis Rehder extract decreased expression of the HCMV IE gene in virus-infected HEL cell.
Conclusion: These results suggest that Sasa senanensis Rehder extract have anti-HCMV activity by inhibitting of IE gene expression.
2.Anti-Human Cytomegalovirus Activity of Chemical Constituents from Kumazasa Hot Water Extract
Kazuhiko AKUZAWA ; Rie YAMADA ; Changxiao BI ; Hidetaka SADANARI ; Keiko MATSUBARA ; Yuuzou TSUCHIDA ; Kunitomo WATANABE ; Masayuki NINOMIYA ; Mamoru KOKETSU ; Tsugiya MURAYAMA
Japanese Journal of Complementary and Alternative Medicine 2010;7(1):25-33
Objective: Anti-human cytomegalovirus (HCMV) activity of 5 compounds, especially tricin, isolated from Kumazasa extract were investigated in the human embryonic lung (HEL) fibroblast cell.
Method: This study examined the effect of tricin on HCMV replication, viral-specific major immediate early (IE) gene expression, and protein synthesis.
Results: Pretreatment of HEL cells before HCMV infection with tricin inhibited viral production in a dose-dependent manner, as well as posttreatment of HCMV-infected HEL cells with tricin. Real-time RT-PCR assay and Western blotting analysis indicated that tricin decreased expression of the HCMV IE gene in virus-infected HEL cell.
Conclusion: These date suggest that the primary mechanism of anti-HCMV activity for tricin, isolated from Kumazasa hot water extract, inhibit IE gene expression.