The research and quality control of traditional Chinese medicine has meaningful importance, because it has influence not only in the health and treatment of patients, but also in the solid growth and development of phar-maceuticals companies. In some cases, for the complex of TCM, the common QC method on single or multi-target compounds can't really and truly disclose the quality of the Chinese materia medica. Therefore, a lot of researchers do plenty of works to make clear the effectiveness basis, to improve the quality and realize the modernization of TCM. All of these works close together with modern analysis and separation technology. In this article, a novel analy-sis technology-UltraPerformance Convergence Chromatography (UPC2) based its characters and applications should be introduced. It should be a helpful technology for the TCM researchers to facilitate the study and QC works.

AIM: To investigate the changes of vascular endothelial growth factor (VEGF) in the pulmonary circulation of rats with different hypoxia and hypercapnia duration. METHODS: Forty SD rats were randomly divided into normol control group (N), exposed to hypoxia hypercapnia for 2 weeks group (T), for 4 weeks group (F), for 8 weeks group (E). The levels of VEGF were measured and the ultrastructure of pulmonary arterioles was observed by electron microscopy. RESULTS: Mean pulmonary arterial pressure (mPAP), weight ratio of RV to LV+S, the levels of VEGF in serum and lung tissue, the expression of VEGF and VEGF mRNA in group T, F, E were significantly higher than that in group N. With the prolong duration, base of endothelial cell was narrowed, proliferation of smooth muscle cells and collagenous fibers of pulmonary arterioles in rats were increased gradually. CONCLUSIONS: Hypoxia and hypercapnia increase the expression of VEGF mRNA and synthesis of VEGF. VEGF may play an important role in the pathogenesis of hypoxia pulmonary hypertension and reconstruction of pulmonary artery.

Objective To study the expression of vascular endothelial growth factor(VEGF)in hepatic tissue of acute liver failure(ALF)rats after transplantation of microencapsulated hepatocytes.Methods The ALF model of rats was induced by D-galactosamine(D-Gal)1400 mg/kg.Then the ALF rats were divided into three groups 18 h after injection of D-Gal:ALF model group,free hepatocyte transplantation group,and microencapsulated hepatocyte transplantation group,50 rats in each group,respectively.Each group was divided into 7 subgroups,and the rats were sacrificed at 24,36,48,72,120,168 and 240 h respectively after injection of D-Gal.The expression of proliferating cell nuclear antigen(PCNA) and VEGF in liver tissue of ALF rats was detected by immunohistochemistry method.VEGF mRNA in liver tissue was detected by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR).Comparison among groups was done by one way ANOVA.Results Compared with ALF model group,the expression of PCNA protein and in free hepatocyet transplantation group in microencapsulated hepatocyte transplantation group began to increase obviously at 36 h after injection of D-Gal(F=26.26,P<0.05).Compare to that of ALF model and free hepatocyte transplantation group,the expression of VEGF protein in microencapsulated hepatocyte transplantation group increased significantly at 36 h after injection of D-Gal(F=25.44,P<0.05),which decreased slower and was still higher at 240 h after injection of D-Gal(F=220.25,P<0.05).Compared with ALF model group,the expression of VEGF mRNA in free hepatocyte transplantation group increased obviously at 24 h(48.0±1.9 vs 56.7±9.1;F=3.54,P<0.05).And that in microencapsulated hepatocyte transplantation group was higher than free hepatocyte transplantation group at 48 h(100.7±1.9 vs 94.5±1.4;F=47.82,P<0.05),which was still significantly higher than free hepatocyte transplantation group at 240 h after injection of D-Gal(F=21.70,P<0.05). Conclusion Transplantation of microencapsulated hepatocytes could upregulate the expression of VEGF in liver tissues and promote the regeneration of hepatocytes in ALF rats.

Objective: To investigate the stamina,the role of antioxidant system in severe acute pancreatitis(SAP),and the effect of fish oil treatment in rats.Methods:Thirty-six male Sprague-Dawley rats(SD) were randomly divided into three groups,including SAP group(sodium chloride treatment ) (group NSG,n=12),fish oil treatment group (group FOG,n=12),and control group (group CG,n=12).SAP was induced by intergraded injection of 3.5% sodium tanrocholate to biliopancreatic duct of SD rats in group NSG and FOG.The group NSG rats were treated by subcutaneous injection of sodium chloride,while the group FOG rats were treated by subcutaneous injection of fish oil for 7 days.Then the improved tail suspension test was observed at the first,third,fifth,seventh day.12 rats in each group were respectively sacrificed after 7 day.The activity of serum antioxidant enzymes (MDA,GSH-PH) and the concentration of serum amylase were measured in each group,and the levels of threshold on the area and the total immobility time were measured in each group.The severity of pancreatitis was analyzed according to the histopathological morphology.Results: Compared to group NSG,the severity of pancreatitis was significantly decreased in group FOG.The activity of MDA was significantly increased in group NSG than that in group CG (P<0.01) ,while the activity of MDA in group FOG was decreased than that in group NSG (P<0.05) .The activity of GSH-PH was significantly decreased in group NSG than that in group CG(P<0.01),while the activity of GSH-PH was increased in group GOG than that in group NSG (P<0.05).THE level of threshold on the area was decreased in group NSG than that in group CG(P<0.01),while the level of threshold on the area was increased in group GOG than that in group NSG.(P<0.01) The total immobility time was significantly increased in group NSG than that in group CG(P<0.01),while the total immobility time was decreased in group GOG than that in group NSG(P<0.05).MDA was associated with the level of threshold on the area and the total immobility time.Conclusion: Fish oil has a positive effect on the activity of antioxidant system and behavior character in SAP rats.