1.Evaluation of antibody response of sheep to foot-and-mouth disease vaccine prepared by using different MontanideTM oil adjuvants
Cokcahskan, C. ; Turkoglu, T. ; Tuncer-Goktuna, P. ; Sarreyupoglu, B. ; Aras-Uzun, E. ; Gunduzalp, C. ; Kurkcu, A. ; Kara, O. ; Karakaya, M. ; Kulyaz, V.
Tropical Biomedicine 2021;38(No.1):154-159
Despite the widespread use of the conventional inactivated foot-and-mouth disease (FMD)
vaccine, its immunogenicity is poor and the duration of its protection is short. In this study,
humoral response to commercial ready-to-use MontanideTM ISA 201 VG and MontanideTM
ISA 61 VG oil adjuvants and a common adjuvant MontanideTM ISA 206 VG developed by Seppic
Inc., were evaluated for FMD antigens in sheep and double oil emulsion (w/o/w) formulations
of MontanideTM ISA 201 and 206 and single oil emulsion (w/o) of MontanideTM ISA 61 have
been prepared by using current FMDV antigens (O/TUR/07, A/ASIA/G-VII, A/TUR/16 and ASIA/
TUR/15). The animals (n=48) were vaccinated subcutaneously with formulations and five
sheep were maintained as an unvaccinated control group. Blood samples were taken at day
0, 7, 14, 21, 28, 60, 90, 120 and 150. Virus neutralization and liquid phase blocking ELISA tests
were used to compare antibody response to vaccines prepared by using different MontanideTM
mineral oils. The results showed that vaccines prepared by using MontanideTM ISA 61 and
201 gave better antibody response to FMD antigens than MontanideTM ISA 206 formulation,
although results were not statistically significant for certain days of sampling. Moreover,
the overall type O antibody response of MontanideTM ISA 201 was found to be superior to
MontanideTM ISA 61.
2.Determination of the antimicrobial property of oregano (Plectranthus amboinicus (Lour.) Spreng.) crude aqueous leaf extract against throat pathogens using broth and checkerboard dilution methods.
Sofia Isabel T. Manlubatan ; Kara Mae H. Matias ; Kenneth Roy P. Mendoza ; Sheryll Anne R. Manalili ; Kris Conrad M. Mangunay ; Christine Mae G. Olivar ; Johnnel G. Pahila ; Greco Mark B. Malijan ; Paulo Miguel G. Manzanilla ; Marie Abigail M. Marin ; Ramon Joseph Y. Mata ; Abdel Hadi M. Mohammad Isa ; Carla Maja Lizl A. Montañ ; a ; Maika Kamille M. Mortell ; Mark Terence P. Mujer ; Patricia A. Nacianceno ; Maria Regina Rocio S. Naval ; Joseph V. Orañ ; o ; Gabriel M. Ozoa ; Alfredo P. Pacheco ; Leonila E. Casanova ; Cleotilde H. How
Acta Medica Philippina 2022;56(5):6-16
Objective: This study aimed to determine the antimicrobial property of oregano (Plectranthus amboinicus) crude leaf extract against pathogens that infect the throat, specifically Streptococcus pyogenes and Candida albicans using the broth and checkerboard dilution methods.
Methods: This study employed an experimental study design using broth dilution method for the determination of the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) against Streptococcus pyogenes ATCC 19615, and minimum fungicidal concentration (MFC) against Candida albicans ATCC 14053 of P. amboinicus crude extract (PA extract). Checkerboard dilution method was then used for determination of the synergy between PA extract and the standard antimicrobials.
Results: In vitro growth inhibition of S. pyogenes (MIC 0.13 g/mL) and C. albicans (MIC 0.03 g/mL) was exhibited by the PA extract. The highest concentration of PA extract used in this study was not sufficient to demonstrate bactericidal and fungicidal activity (MBC >0.25 g/mL, MFC >0.25 g/mL). Results of checkerboard dilution method revealed that PA extract when combined with either penicillin (for S. pyogenes) or nystatin (for C. albicans) demonstrated indifference.
Conclusion: The crude extract of Plectranthus amboinicus has the capability to inhibit the growth of both S. pyogenes and C. albicans. This demonstrates its potential use in the treatment of throat infections caused by these organisms.
Key Words: Plectranthus amboinicus, oregano, antimicrobial, throat infections, herbal medicine
Origanum ; Herbal Medicine
Result Analysis
Print
Save
E-mail