Birds,especially wild waterfowls,are a natural reservoir of influenza virus,though poultry,mammalians and even human beings are also thought to be the potential hosts.Influenza virus can be classified into subtypes according to the characteristics of the nucleocapsid protein and membrane protein,each subtype with its own most adaptable host,so called host range restriction.However,the outbreak and spreading of influenza in different species has aroused increasing attention since its disastrous consequence on the ecological system of the earth.Large-scale epidemic survey,prediction of possible mutation of the virus,development and application of vaccines are key actions to be taken to prevent the pandemic of influenza.This article offers an overview of the ecology of influenza virus,features of recent great outbreaks of avian influenza,and relevant issues of concern.

In this paper, it was reported that rat thymocytes harbor both high and low affinity ACTHR. The proper number of thymocytes used for deterting ACTHR varies from 1?10~6 /0.2 ml to 2 ?10~6 /ml, the maximum binding of ACTH to ACTHR occurs at 20 minutes. The IC_(50) for ACTH concentration is 800 nM or so. The saturation curves show that thymocyte ACTHRs are saturated at the concentration of 2.5 nM of ~(125)IACTH. There are high affinity ACTHR of 840 sites/cell on thymocyte (KD=0.15nM, Bmax = 2. 8 fmol/2 ? 10~6 cells); whereas low affinity ACTHRs are 12400 sites/cell (KD=4.2 nM, Bmax = 41.5 fmol/2 ? 10~6 cells).Moreover amputation stress can reduce thymocyte ACTHR remarkably.

The pandemic outbreak of influenza has been started from Mexico in 2009 to 70 countries during 2 months. On 11th of June , WHO announced influenza pandemic alert level rose to the highest level 6, which means the first influenza pandemic in 21st century is coming. Till 6th of July, 94 512 confirmed cases from more than 120 countries and areas were reported, including 429 cases were died. The genetic fragment of swine, poultry sources and human influenza viruses are contained in this strain, A/H1N1 influenza virus, of the pandemic. It is of great significance of studying the genetic reassortment, evolution and its biological characteristics of this virus strain to prevent and control the pandemic. At present, the genetic evolution of strain has been identified, and the potential biological characteristics have been analyzed by genetic traits, however, clinical manifestation should be further concerned, and the tendency of influenza pandemic and genetic changes need to be monitored closely. The complexity of influenza virus ecosystems, mutation of genome, and easy to preserve in "Nature Gene Pool" and reassortment, make the influenza pandemic inevitable. We should face the threat of influenza pandemic, enhance the surveillance of influenza virus in ecosystems, strengthen the epidemiological investigation, develop the vaccines and drugs, and establish an effective public health security system, in order to reduce the destruction of the influenza pandemic.

A variety of molecular markers are related to the prognosis of gastric cancer,such as the the loss expression of AT rich interactive domain 1A (ARID1A),the overexpression of CD133 and survivin are releated to the incidence and poor prognosis of gastric cancer; leptin,CD44 and microRNA (miRNA) are releated to the invasion and metastasis of gastric cancer; the low expression of secreted protein acidic and rich in cysteine (SPARC) contributes to the angiogenesis of gastric cancer,but the relationship between SPARC and prognosis needs further study; the expression and gene polymorphism of excision repair cross-complementing 1 (ERCC1) may be useful for choice of medication in the treatment of gastric cancer.

Objective To observe the clinical curative effect of NB -UVB combined with prednisone on neuralgia following herpes zoster .Methods 84 patients with neuralgia following herpes zoster were randomly divided into treatment group and control group , with 42 in each.The treatment group were given NB -UVB 3 times weekly, and oral prednisone tablet 10mg three times daily for 14 days.The control group was treated with prednisone tablet 10mg three times and indomethacin 25 mg two times daily for 14 days.The visual analogue scale (VAS) was used to evaluate the therapeutic effects in the two groups .Results The effective rate of the treatment group was 88.1%, and that of the control group was 69.05%.The therapeutic effect in the treatment group was statistically significant higher than that of the control group (χ2 =5.974, p=0.015).No marked side effects were observed in both groups .Con-clusion NB-UVB combined with prednisone is effect in the treatment of neuralgia following herpes zostert .

Objective:To investigate the effects of brain asymmetry on levels of IL-1?,IL-6 and suppressor of cytokine signaling-3(SOCS-3) in hypothalamus,to get better understanding of relationship between brain asymmetry and neuro-immuno-endocrine network at the molecular level.Methods:By using paw preference test,right-pawed,left-pawed,and ambidextrous mice model was established.After decapitation of the mice,hypothalamus was separated rapidly.Some samples were homogenized and used for detection of levels of interleukin-1?(IL-1?),interleukin-6(IL-6) by Enzyme-Linked-Immunosorbent Assay(ELISA).The other samples were applied to detect SOCS-3 expression level by RT-PCR.Results:(1)The level of IL-6 in hypothalamus:left-pawed were higher than right-pawed mice,there was significant difference in statistics( P 0.05).(3)The expression level of SOCS-3:the SOCS-3 expression of right-pawed showed higher than left-pawed mice in hypothalamus,revealing a significant difference( P

AIM: To investigate the influence of predator stress on hypothalamic-pituitary-adrenal (HPA) axis and IL-1?, IL-6 in mice with brain asymmetry. METHODS: By using paw preference test, right-pawed, left-pawed, and ambidextrous-pawed mice model were established. Mice with brain lateralization were exposed to their predator (cat). After acute and chronic predator stress by cats, EIA and ELISA were applied to detect plasma levels of corticosterone (CS), IL-1? and IL-6. RESULTS: (1) The level of plasma CS: in both acute and chronic predator stress group, right-pawed and ambidextrous mice had a higher level than that in their corresponding normal group (P

Objective To investigate the properties of cultured periosteal cells associated with bone morphogenetic protein(BMP) to find a new treatment for bone defects.Methods The periosteal cells were cultured,expanded and passage cultured in vitro to investigate the characteristics and functional changes of rabbit periosteal cells.Animal models of 1.0cm gaps with complete removal of the bilateral radius medium bone and periosteum were induced in 24 New Zealand rabbits,which were randomly divided into 4 groups to receive the defect repair with periosteal cells combined with BMP,periosteal cells,BMP and blank control respectively.X-ray and histological examination were made periodically after operation to evaluate the effectiveness of bone formation.Results Periosteal cell morphology can be divided into three stages: cell division phase,cell growth phase and cell functional phase.Cellular configuration had no significant difference with primary cells after anagenesis.The callus can be formed after the periosteal cells were transplanted into the bone defection.The osteogenesis ability of periosteal cells combined with BMP was better than that of the BMP,and the osteogenesis ability of the BMP was better than that of the periosteal cells.The mechanism of bone regeneration for periosteal cells was similar to intramembranous ossification,and that of BMP was similar to endochondral ossification.In the periosteal cells combined with BMP group,both intramembranous and endochondral ossification existed,and the former was obvious.Conclusion Both periosteal cells and BMP can improve the repair of bone defect in vivo.Cultured periosteal cells combined with BMP had substantial ability of synergistic osteogenesis,and it was an ideal treatment for bone defection.

Objective:To clarify the role of Aire on the production of regulatory T cells on mice.Methods:The distribution of CD4~+CD25~+T cells and the expression of Foxp3 mRNA from Aire~ -/- thymus and spleen were separately analyzed using FACS and real-time PCR.Results:Compared with control mice, the total numbers of thymocytes/splenocytes and T cells from Aire~ -/- mice did not show any significant changes; The total CD4~+CD25~+ regulatory T-cell number and Foxp3 mRNA expression did not exhibit any statistically difference; The ratios of CD4~+CD25~+T cells out of CD4~+T cells were similar based on adult /day3/day7 mice analysis.Conclusion:Aire gene does not affect the production of CD4~+CD25~+ regulatory T cells.

Objective To investigate the clinical roles of lysine methyltransferase 5A(KMT5A)in human hepatocellular carcinoma(HCC)and its functions in cell migration and invasion. Methods The expression levels of KMT5A of 60 cases were detected by immunohistochemistry(IHC). KMT5A siRNA was used to down-regulate the expression of KMT5A in SMMC-7721 cells. Cell migration and invasion were measured by wound healing assays and transwell assays,respectively. Immunoblotting was used to detect the expression of MMP-2 after siRNA trans-fection. miR-186 mimics were transfected into SMMC-7721 cells and the mRNA levels of KMT5A was detected by qRT-PCR after transfection. Results High expression of KMT5A was associated with large tumor diameter (>5 cm,P=0.047)and advanced TNM stage(Ⅲ+Ⅳ,P=0.035). The expression of KMT5A was knocked down by siRNA in SMMC-7721 cells. Down-regulation of KMT5A suppressed cell migration(P=0.031,P=0.006)and invasion(P=0.010),and impaired MMP-2 expression(P=0.040). Overexpression of miR-186 could significantly inhibit the expression of KMT5A(P = 0.007). Conclusions Over-expression of KMT5A in HCC tissues associ-ates with poor clinical features. KMT5A knockdown inhibits the migration and invasion on HCC cells.