Anti-Bacterial Agents ; therapeutic use ; Communicable Diseases ; drug therapy ; Diagnosis, Differential ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Fever ; drug therapy ; etiology ; Humans ; Medicine, Chinese Traditional ; Meningitis, Meningococcal ; drug therapy ; Phytotherapy

By using immunocytochemistry (the whole mount stretch preparations and cryostat sections) and radioimmunoassay, the characteristics of distribution of calcitonin gene-related peptide (CGRP) in small intestine of rats and its change in acute intestinal radiation sickness are studied.The results show that all layers of the small intestine are rich in CGRP-immunoreactive(CGRP-I)nerves with a high density in the myenteric, submucosal and mucosal plexuses. Also it has a rather high density around the small blood vessels of the small intestine and intestinal crypts.Same CGRP-I neurons are seen in the myenteric and submucosal plexuses.In AIRS the level of CGRP in small intestine has a double-phase change which is lower at the 24th h followed by a higher level at 48th h, 72th h after the exposure. The results indicate that CGRP may be related to the regulation of motility, secretion,absorption sensation and regional blood flow of the gastrointestinal tract. CGRP is probably released under the stress of AIRS and participates in the mechanism of injury of AIRS through multiple ways, in particular, the influence on the regional blood flow and the increase of permeability of blood vessels.

By using immunocytochemistry (the whole mount stretch preparations and cryostat sections) and radioimmunoassay, the characteristics of distribution, origins and properties of calcitonin gene-related peptide (CGRP) were studied. The results show that all layers of small intestine were rich in CGRP-immu-noreactive(CGRP-I)nerves with a high density in myenteric, submucosal and mucosal plexuses. Also it had a rather high density around the small blood vessels of the small intestine and intestinal crypts. Some CGRP-I neurons were seen in the myenteric and submucosal plexuses which were the intrinsic origin of CGRP-I nerves of small intestine. And the denervation and injection of capsaicin demonstrated that the extrinsic CGRP-I nerves of small intestine with mainly sensory origin also existed. The characteristics of distribution of CGRP-I nerves in small intestine indicated that the CGRP-I nerves might be related with the regulation of motion, secretion, absorption, sensation and regional blood flow of the gastrointestinal tract.

Adult ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Female ; Humans ; Infertility, Female ; drug therapy ; Ovulation ; drug effects ; Ovulation Induction ; Phytotherapy

Objective To investigate and evaluate the biomechanical property of the skin in pig's back in order to provide the essential theoretical basis for clinical and skin products.Method Taking the skin in pig's back as experimental material,the monotonic tensile and cyclic tension-tension tests with difierent loading rates was researohed respectivaly.Meanwhile,with different loading directions and stress levels the creep and cy-clic tension-tension tests were also been studied experimentally.Result The capacity of resisting tensile,creep and cyclic deformation of pig's skin in the direction along the Langer's line is stronger than that perpen-dicuiar to the Langer's line.The creep curve of pig's skin is load-dependent and consisted of three phases a-bout deceleration phase,stabilization phase and destruction stage.Pig's skin exhibits apparent ratcheting un-der asymmetry stress cycle.Ratcheting deformation displays significant mean stress,stress amplitude and loading speed dependence.Condusion Based on the experiment,the biomechanics property of skin's vis-coelasticity and anisotropic feature have been sysmarie stadied,it's provide necessaw theoretical fundation for clinical and leather products.

Small RNA (miRNA) can regulate post-transcriptional level of mRNA. miRNA is closely related to the occurrence and development of a variety of human diseases such as gastric cancer, lung cancer, breast cancer, ovarian cancer, prostate cancer, pancreatic cancer, liver cancer, etc. Many kinds of miRNAs in human cancers are significantly abnomadly expressed. miRNA can be regarded as a marker for many human cancers diagnosis.

Objective: Spinal muscular atrophy(SMA), an autosomal recessive neuromuscular degeneration of the anterior horn cells of the spinal cord and brain stem, results in one of the most common diseases with muscle fatigue and atrophy. Most SMA cases including all the types are due to the homozygous deletion of at least exon 7 within the survival motor neuron 1 (SMN-1) gene. Although a "golden standard" assay (PCR with mismatch primer followed by enzyme digestion) is very reliable for the identification of homozygous SMN-1 deletion, the carrier detection of heterozygous SMN-1 deletion remains a challenge. Methods: Some PCR-based gene dosage assays or multiplex PCR allow for the determination of the copy number of SMN-1 gene to identify heterozygous deletion, but these procedures are often time consuming and available on a limited clinical basis. Recently developed MLPA (multiplex ligation-dependent probe amplification) is an efficient procedure that can accurately analyze relative quantification to establish the copy number of the SMN gene. We performed a validation for simultaneous detection of homozygous SMN-1 deletions of SMA patients and heterozygous SMN-1 deletions of SMA carriers in a simple assay using a MLPA-SMA assay specific reagent. Results: Six out of 20 patients with SMA were found to have homozygous SMN-1 deletion, confirmed by the PCR/digestion assay. All 4 parents of the children with SMA had heterozygous SMN-1 deletion, confirmed by an independent relative quantitative analysis. Conclusion: MLPA provides a simple, rapid and accurate method of simultaneously detecting homozygous deletions and heterozygous deletions in a single assay for both SMN-1 and SMN-2 genes.

OBJECTIVE:To analyze the components of the volatile oil in different parts of Lonicera japonica Thunb.from Henan province.METHODS:The volatile oil was extracted from the buds,leaves,stems of L.japonica Thunb.by solid-phase micro-extraction,and the chemical compositions were identified by GC-MS combined with Kvotas retention index.The relative percentage of each constituent was determined by GC area normalization method.RESULTS:Thirty-nine compounds were identified from different parts of L.japonica Thunb.from Henan province,and seven of which were mutual in the buds,leaves,stems of L.japonica Thunb..CONCLUSION:This study serves as a scientific basis for the further development and utilization of L.japonica Thunb..

OBJECTIVE: To analyze the volatile components in the Eriobotrya japonica by headspace solid-phase microextraction-gas chromatography-mass spectrometry(SPME-GC-MS) and to optimize the operating conditions for SPME.METHODS: DB-5 MS elastic silica capillary column(30.0 m?250 ?m?0.25 ?m) was used as the chromatographic column with helium of high purity(99.999%) as carrier gas at a flow rate of 1.0 mL?min-1.The injector temperature was 250 ℃;MS condition: ionization mode was EI with electron energy of 70 eV and the mass scan range of 30～440 amu.RESULTS: After headspace absorption for 30 min at 80 ℃ using 65 ?m polydimethylsiloxane-divinyl-benzene extraction head followed by desorption for 1 min at 250 ℃,the E.japonica sample was separated and identified by GC-MS analysis.A total of 34 peaks were obtained and 29 components were identified which accounted for 97.42% of the total peak area,topping the list were benzaldehyde and 4-methoxybenzaldehyde.CONCLUSION: HS-SPME-GC-MS can be used for the rapid analysis of the volatile components in E.japonica.

OBJECTIVE:To conduct a quantitative analysis on the volatile constituents from the flower of Tamarix chinensis. METHODS:The volatile constituents were extracted from the flower of T. chinensis by solid-phase microextraction technique and component identification was carried out by GC-MS combined with retention index. RESULTS:36 kinds of compounds were identified,among which pentadecane took up a greatest proportion (9.80%),followed by 6,10,14-trimethyl-2-pentadecanone (7.61%),5,6-dihydro-6-pentyl-2H-pyran-2-one(6.83%),hexadecane(6.25%)and 5,6,7,7a-tetrahydro-4,4,7a-trimethyl-2(4H)-benzofuranone(5.13%).CONCLUSION:This study serves as a scientific basis for the further development and utilization of T. chinensis.