AIM: To establish a simple and repeatable orthotopic animal model of the A549 cell line.METHODS: A549 cells in matrigel were inoculated into left lung or right flank of nude mouse. The growth pattern of A549 tumors and the chemotherapeutic efficacy of carboplatinum were studied. RESULTS: Tumors generated and dispersed widespreadly to contralateral lung. Tumors in lung were less susceptible to carboplatinum than those growing in flank. CONCLUSION: A simple and repeatable orthotopic animal model of the A549 cell line was presented, this allows researchers to evaluate novel compounds and new drugs for treatment of human lung cancer.KEY WORDS A549 cell line; orthotopic model; nude mice

Objective To study the effect of new generation histone deacetylase inhibitor LBH589 single-drug or combined with the mouse serum which contains the radix echinopsis on multiple myeloma (MM) cell line U266 and their mechanism.Methods The acetylation level of α-tublin which was a substrate of HDAC6 was detected by Western blot.The chemical force between Heat shock protein 90 (HSP90) and its client proteins was detected by immune precipitation (IP).Results The different concentrations of LBH589 single drug (0,20,50 nmol/L),and 50 nmol/L combined with the mouse serum which contained the radix echinopsis (1 g/ml) were able to inhibit the proliferation of U266 cell.With the increase of drug concentration and the extension of time,the acetylation levels of α-tublin and HSP90 increased gradually (at 24 or 48 hours) in a dose dependent (P ＜ 0.05).The inhibition of LBH589 combined with the mouse serum was stronger than that of LBH589 single drug (P ＜ 0.05).Conclusion LBH589 could inhibit the growth of MM cells and their cell cycles,and induce the apoptosis of MM cell line U266.

Cardiopulmonary resuscitation is an important part of standardized residency training. There are certain flaws in the various commonly used training and assessment mode. In the exploration of the new mode, "step-by-step" training helps to learn skills step by step. "Low intensity and high frequency" training not only focuses on key points and difficulties, and lightens the burden of the teachers and residents, but also can improve the training effect through repeated reinforcement. Combining different training methods organically to establish a "diversified" training mode can make full use of the advantages of different methods and make up for each other's shortcomings. In addition, the "veto power" should be introduced into the assessment. Scenario simulation with role play may be an effective way to train teamwork ability, but it still needs further exploration.

Objective To understand the knowledge of sexual and reproductive health of undergraduates with different nationalities and provide scientific basis for sexual and reproductive health education in the nationalities university.Methods A self-developed questionnaire about sexual,reproductive health and sexual attitude was used to carry out investigation among 2 200 freshman.Results Formal education (family and school) do not become the main source of the sex knowledge; there were statistic significance of the comparison of sexual,reproductive knowledge and sexual attitude among minority undergraduates and Han nationality undergraduates,as well as among undergraduates from different minorities.Conclusions The undergraduates are thirsty for gaining general sexual knowledge from school and family during the period of university.It is necessary to improve sexual and reproductive knowledge through various ways for minority undergraduates.

On the basis of Shay's ulcer model, we have studied the effects of food re. striction time on the experimental gastric ulcer in rats. The results indicat that each restricted group forms gastric ulcer after ligation of pylorus 18 hr. There are a postive correlation between the restricted time and the severity of gastric ulcer, and a negative correlation between the severity of gastric ulcer and the amount of gastric acid secretion. We suggested that Shay's ulcer model does not belong to peptic ulcer, but belonges to one of the stress ulcers. The restricted time may play an important role in the mechanism for this ulcer model.

Objective To determine the impact of body mass index(BMI)on clinical outcomes in patients underwent coronary revascularization.Methods The DESIRE-plus(Drug-Eluting Stent Impact on Revascularization-plus)was a single-center registry study of coronary revascularization in our institution between 1 July 2004 and 30 September 2005.We analyzed patients with complete data of BMI from the DESIRE-plus trial,grouped by BMI(low BMI group:BMI

Objective To evaluate the advantage of laparoscopic total mesorectal excision (TME) and the clinical value of pull-through anastomosis in the treatment of rectal carcinoma. Methods From November 2005 to December 2006, laparoscopic TME combined with pull-through anastomosis was carried out on 8 patients with rectal cancer. Results In all of the 8 patients, TME was completed under a laparoscope without additional incision, and the pull-through anastomosis was performed by hand.The mean operation time was 220 minutes (range, 180 to 300). The patients were followed up for 8-18 months (mean, 13 months), during which none of them developed implantation at puncture sites or local recurrence. Conclusions Laparoscopy provides a direct, magnified and reliable view for TME. Pull-though anastomosis is a convenient and low-cost method.

Objective To investigate and compare the short-term and long-term outcomes between percutaneous coronary intervention(PCI) or coronary artery bypass graft(CABG) in coronary heart disease(CHD) patients comorbid with diabetes mellitus.Methods The DESIRE register study(Drug-Cluting Stent Impact on REvascularization) was a single-center retrospective study which enrolled 3763 patients who received revascularization therapy during two distinct pesiod.Our study included 670 CHD patients from the whole DESIRE population who received revascularization during July 2003 to June 2004 and comorbid with diabetes mellitus.According to the revasularizaton strategy,the patients were divided into the PCI group(n=400) and the CABG group(n=270).Adverse cardiac and vascular events were the composite endpoints which included all-cause of death,non-fatal myocardial infarction,non-fatal stroke and revascularization.Results Baseline data had no difference between the two groups.In-hospital adverse cardiac events in the PCI group was fewer than in the CABG group(0.5% vs 4.1%,P=0.001) as shown by a lower mortality in the PCI group compared with the CABG group(0.5% vs 3.3%,P=0.012).Duration of follow-up in the PCI group and the CABG group was 592.6?121.3 days and 581.5?148.3 days respectively.The incidence s of long-term adverse cardiac events had no differnce between the two groups,but an increase trend was observed in the PCI group compared with the CABG group(14.2% vs 9.0%,P=0.056),owing to a higher percentage of revascularization(8.5% vs 2.1%,P=0.001).Two groups had a similar incidence of death,non-fatal myocardial infarction,non-fatal stroke during the follow-up period.Conclusion With the wide-spread of DES application,in-hospital adverse cardiac events in the PCI group was fewer than in the CABG group especially in terms of mortality.Long-term adverse cardiac events had no differnce between two groups,but PCI group had more revascularization.

Aim To determine whether there existed melatonin receptor (MR) in adult hepatoma tissue and to observe its property of binding.Methods Immuno-histochemistry was used to detect MR and identify its subcellular distribution. Specific binding and kinetic analyses of melatonin receptor were measured by radioligand binding assay.Results The results of immunohistochemistry showed that MT1 and MT2 existed in membrane, cytosol and nucleus of the hepatoma cells, but the expression of them principally localized in the membrane and cytosol. The specific binding assay properties of MR were presented as follows: the maximum binding capacity (B_(max)) was (0.29±0.07) pmol·g~(-1) protein. Equilibrium dissociation constant (K_d) was (48.7±6.5) pmol·L~(-1). The trait of MR, saturation and reversibility, was detected by ~(125)I-Mel specific binding kinetic analyses.Conclusions MR exists in adult hepatoma cells, furthermore, the subtypes of MR (MT1 and MT2) coexist in the membrane and cytosol, respectively,whose characters of the specific binding sites were low binding capacity,high affinity,saturation and reversibility.

Objective To study the quality control of yinpian of Isatis Root.Methods Optical microscope was used to identify the microscopic features,TLC was adopted to identify the arginine in Isatis Root,and HPIC-ELSD was used to detect the quality of arginine.Results and Conclusion There is no difference between yinpian and crude powder of Isatis Root.Arginine can be detected bv TLC.The moisture contents.the total ash contents and the acid-insoluble ash contents in the yinpian should be less than 9.0%,10.0%,and 2.0% respectively;while the alcohol-soluble extractive contents and the contents of arginine in Isatis Root should be more than 25.0% and 2.01% respectively.