RESULTS AND CONCLUSION:(1) Motor function: The motor function scores were better in the acupuncture + rehabilitation group than in the model, acupuncture, and rehabilitation groups (P< 0.05). (2) Growth associated protein-43 and synaptophysin expression: immunohistochemical staining revealed that compared with the model group, growth associated protein-43 expression at 2-4 weeks and synaptophysin expression at 4 and 5 weeks were higher in the acupuncture and acupuncture + rehabilitation groups(P<0.05); the increase in above expression was most significant in the acupuncture + rehabilitation group (P<0.05). (3) Results confirmed that acupuncture combined with rehabilitation promoted growth associated protein-43 and synaptophysin expression in rats with cerebral palsy and exerted neuroprotective effect.

The study of traditional Chinese medicine and its complex prescription gradually adopted the methods of serum pharmacology, which has become a new researching thought and a gained more attention. In this article, the background of relevant researches, methodological discussions, analysis of the advantages and problems of serum pharmacology were reviewed. The serum pharmacology, for its unique value, will certainly has a brilliant prospect in its application to the research of traditional Chinese medicine and its complex prescription.

Sentinel lymph node biopsy has been an important part of breast cancer surgery,which includes reactive dye method,radionuclide method and the combination of both. However,the radionucli-ded tracer is not approved domestically,which limits its clinical application. Near-infrared fluorescent method depends on near-infrared light. It activates indocyanine green to produce fluorescence and then flu-orescent image device can be used to observe the lymphatic system,which is a real time and dynamic de-tection for lymphangion and lymph node. Furthermore,the definite incision site can be located,which less-ens the damage of tissues. The fluorescent method can avoid some disadvantages of the above methods. However,the shadowless lamp should be turned off during the operation,which prolongs the operation time. Due to the quenching effect,the brightness of fluorescence will attenuate and the detected depth will be limited. Combination of near-infrared fluorescence method and reactive dye method may improve the detection,but further large-scale clinical trials are required for confirmation.

Immunocompromised hosts(ICH)is a particular population drawing more current attention with the susceptibility to infections,especially to the opportunistic pathogens.When infections occur in ICH,the mortality increases significantly.The clinical manifestations and radiological characteristics are untypical and non-specific,so the correct diagnosis in good time is arduous.But the rapid and correct diagnosis is primary and principle to lower the mortality of the infections of ICH,so this article aims to generalize the diagnosis phronesis of pulmonary infections of ICH-to elaborate the key points of the assessment and evaluation of ICH;the principle of differential diagnosis of pulmonary infections in ICH;clinical evaluation program of the pulmonary infiltration in ICH. Abstract:Summ ary:Immunocomprom ised hosts(ICH) is a particu lar popu lation draw ing more current attention w ith the susceptib il-ity to infections,espec ially to the opportun istic pathogens.W hen infections occur in ICH,the mortality increases sign ifi-cantly.The c lin icalm an ifestations and rad iological characteristics are untyp ical and non-spec ific,so the correct d iagnosis in good tim e is arduous.But the rap id and correct d iagnosis is prim ary and princ ip le to lower the mortality of the infections of ICH,so th is artic le aim s to generalize the d iagnosis phronesis of pu lmonary infections of ICH-to elaborate the key points of the assessm ent and evaluation of ICH;the princ ip le of d ifferential d iagnosis of pu lmonary infections in ICH;c lin ical e-valuation program of the pu lmonary infiltration in ICH.

ObjectiveTo assess the microscopic features of alopecia areata(AA) by using in vivo confocal laser scanning microscopy(CLSM).MethodsBetween January 2010 and May 2011,26 male and 20 female patients diagnosed with AA were enrolled in this study.AA lesions and perilesional normal skin were examined by means of in vivo CLSM.Tissue specimens were obtained from the lesions and perilesional normal skin of 10 out of the 46 patients and subjected to routine histopathologic examination.ResultsThe number of hair follicles per unit area(cm2) was decreased in lesions of progressive AA and resting AA compared with normal perilesional skin(134.856 ± 18.301 and 147.159 ± 17.536 vs.301.613 ± 35.317,both P ＜ 0.05).Although the quantity of hair follicles increased in lesions of recovery AA((227.778 ± 16.861 )/cm2),but was still less than that in the normal perilesional skin (P ＜ 0.05).There was a lack of hair shaft in follicles,as well as an inflammatory infiltration in hair follicles,around hair follicles and capillaries in superficial dermis in lesions of progressive AA.The inflammatory infiltration was attenuated in lesions of resting AA.In lesions of recovery AA,the infiltration was further attenuated with an apparent growth of lanugos and terminal hairs.ConclusionsAs far as AA lesions are concerned,CLSM images are consistent with histopathological findings.CLSM may serve as a promising tool for monitoring efficacy and predicting prognosis based on hair growth cycle,hair follicle number,and inflammatory infiltration degree.

Objective: To provide reference for on-site medical emergency preparedness and response to nuclear facilities of our country. Methods: On the basis of laws, regulations and standards as well as our experience and research in this field. Results: To provide effective advice for on-site medical emergency preparedness and response. Conclusion:Introduce unclear accident influence, unclear accident emergency system and so on.

Objective To survey the prevalence of the pulmonary function test in Liaoning province and investigate the cognition of chronic obstructive pulmonary disease（COPD）patients to the test.Methods One hundred and twenty nine comprehensive hospitals of Grade Three and Grade Two have been included to survey the prevalence of the pulmonary function test by telephone.A cross-sectional study was carried out among 206 COPD outpatients to investigate their cognition to the pulmonary function test.Results The total prevalence of the hospitals with the equipment and ability to do pulmonary function tests was only 51.2%.The prevalence of the hospitals of Grade Three（79.4%）was higher than that of the hospitals of Grade Two（24.2%）.In 206 COPD patients,100 cases（48.5%）had been provided with pulmonary function test,53 cases（25.73%）had been diagnosed as COPD in the past.The patients diagnosed as COPD accounted for 33.8% of all chronic bronchitis patients surveyed.The span during the diagnosis of chronic bronchitis and the definitive diagnosis of COPD was 11.79±10.96 years.Conclusion The prevalence of pulmonary function test in Liaoning province is relatively low,especially in the comprehensive hospitals of Grade Two.Few chronic bronchitis patients received pulmonary function test.Thus the time span from the diagnosis of chronic bronchitis to definitive diagnosis of COPD is long as well.

Objective: Our aim was to evaluate the role of TGF-β1 mRNA in pulmonary fibrosis. Methods: The present study was taken to investigate the expressions of TGF-β1 mRNA in alveolar macrophage（AM) and lung tissues in different stages of bleomycin-induced pulmonary fibrosis in rats through reverse transcript polymerase chain reaction. Results: In bleomycin-treated rats, the expressions of TGF-β1 mRNA was remarkably increased in AM from 3rd day (P< 0.05) , and reached the peak on 7th day (P<0.01) , there was also a continuing high level from 14th day to 28th day (P>0.05). It showed similar pattern with that in lung tissues. Conclusion: There were TGF-β1 mRNA expression in both normal rats and pulmonary fibrosis rats. AMs seemed to be the main source of TGF-β1 mRNA in lung tissues, especially at stage of alveolitis.

The oral glucose tolerance test,insulin release test and C peptide relea se test were carried out in 69 hypertensive patients and 55 normotensive subjects. It was found that,the concentrations of blood glucose,serum insulin and C peptide and the occurrence rates of glucose metabolic abnormality(diabetes mellitus and impaired glucose tolerance)of hypertensive group were higher than those of normotensive control group.The co-existence of hyperglycemia and hyperinsulinemia suggests the existence of insulin resistance.

ObjectiveTo study of the impact on dose distribution by electron width of energy spectrum and angular distribution using Monte Carlo simulation.MethodsThe simulated electron energy spectrum and angular distributions was as the input parameters and percentage depth dose (PDD) and offaxis curves were simulated by a modified PENELOPE code package. Results PDD and off-axis dose distribution curves are almost the same and are not sensitive to energy spectrum width and angular distribution with the exception of energy spectrum width of 2.5 MeV with obviously different curves.ConclusionsIn the situation of clinical treatment,spectrum and angular distributions can be ignored when their width are not very large.It is helpful to save about 9％ time by using monoenergy beams in treatment planning system development.