Objective To study the effect of dexmedetomidine on stress response and inflammatory reaction of patients with hysterectomy.Methods 96 patients with hysterectomy who received therapy from October 2014 to October 2016 in Jinhua traditional Chinese medicine hospital were selected.According to random number table,those patients were divided into the observation group(n=48)and the control group(n=48).Two groups were treated with total intravenous anesthesia,the observation group was treated with intravenous infusion of dexmedetomidine1μg·kg-1 before anesthesia,pump is completed within 15 minutes,then continuous injection of 0.5μg·kg-1·h-1 speed to the 30min before the end of the operation; the control group was given the same way of infusion of normal saline.The operation time,blood loss,recovery time,extubation time and length of stay were recorded,and the hanges of mean arterial pressure(MAP),heart rate(HR),hydrogen peroxide(H2O2),malondialdehyde(MDA),total antioxidant status(TAS),tumor necrosis factor(TNF)-α,interferon(IFN)-γ,interleukin(IL)-10 at T0(before anesthesia),T1(at the end of pneumoperitoneum),T2(after the end of pneumoperitoneum 30min),T3(postoperative 24h)were recorded.Results There was no significant difference between the two groups in operation time,blood loss,recovery time,extubation time and length of stay; in the control group,the MAP was significantly reduce at T1,T2 was significantly higher,the HR was significantly reduce at T1(P<0.05); there was no significant change of MAP and HR levels in the observation group at each time point; the H2O2 and MDA in the observation group were lower than that of the control group at T1,T2 and T3,and the TAS was higher than that of the control group(P<0.05); the TNF-α,IFN-γ and IL-10 in the observation group were lower than that of the control group at T1,T2 and T3(P<0.05).Conclusion Intravenous infusion of dexmedetomidine before anesthesia is well for patients with hysterectomy,it'is helpful to maintain the hemodynamic stability,and effectively alleviate the stress response,inflammation reaction,it's worth promoting.

Aim To investigate the relationship between the antitumor activity of berberine and the inhibition of Wnt/?-catenin signaling pathway. Methods The antitumor activities of berberine and total alkaloids of Coptis were assessed for their ability to inhibit the proliferation and to induce apopotosis of human colon cancer lines. A well-established Tcf-4 responsive reporter was introduced into human colon cancer lines to test whether berberine and total alkaloids inhibited the ?-catenin/Tcf4 activity in these cancer cells.Results The proliferation of HCT116 and SW480 cells was inhibited substantially within the concentration ranges of 5 to 40 mg?L~(1) of berberine. 72 hours after treament both HCT116 and SW480 cells underwent apparent apopotosis as demonstrated by Hoechst 33258 staining. Total alkaloids of Coptis had similar effects to berberine when comparable concentrations of it with berberine were used.Berberine effectively and specifically inhibited the Tcf4 reporter activity, but not the positive control reporter at the same experimental conditions. Conclusion Our findings suggest that berberine may be mainly responsible for the antitumor activity of total alkaloids of Coptis and this effect may be mediated at least in part by its inhibitory effect on the Wnt/?-catenin signaling pathway, which is aberrantly activated in many types of human cancer.

Strengthening basic animal operation training is not only beneficial to improving students’ practice ability so as to help students lay a solid foundation for clinic treatment and research work.,but also plays an important role in physiological scientific experiment teaching,which is essential for improving teaching quality. We have an active investigation in this aspect.

OBJECTIVE:To investigate the consumption of drugs after the inplementation of total cost prospective payment sys-tem(PPS),and to provide reference for hospital drug cost control. METHODS:The data of prescriptions collected from 9 hospital during 2007-2014 were divided into group A,B and C according to PPS,and then summarized statistically in respects of increase rate of total consumption sum,consumption sum ratio of major category;the drug cost per time of outpatient department,emergen-cy department and inpatient department were calculated as well as consumption sum ratio of self-paid drugs;the dosage per time of drugs in blood pressure and diabetes prescription were also calculated. RESULTS:The total consumption sum of drug increased slowly after the implementation of total cost PPS;the consumption sum ratio of major category kept stable,while that of anti-infec-tive agent decreased;the emergency drug cost per time achieved a negative growth,and outpatient and inpatient drug cost per time increased slightly. The proportion of self-paid drugs was relatively stable. The dosage per time of drugs in hypertension and diabetes prescriptions was stable,too. CONCLUSIONS:Total cost PPS is useful in controlling the rapid growth of drug costs,and promote the reasonable drug use. The consumption sum of self-paid drugs are well controlled. The increase of drug cost per time in outpa-tient and inpatient should arouse the attention of the relevant departments. In addition,it has no effect on drug dosage for the pa-tients with hypertension and diabetes.

Objective Establishment and characterization of healthy donor's ??T cell clones.Methods ??T cells were cloned by limiting dilution after positive sorting with 60Co irradiated allogeneic PBMC as feeder cells.Flow cytometry analysis and molecular biology technique were then used to identify ??T cell clones.MTT assay was used to verify their proliferation after incubated with epitope peptides recognized by ??T cells.Results A ??T cell clone had been established.The subtype of this clone was V ?9 V ?2 without expression of CD4 and CD8.Further studies indicated that epitope peptide EP6 could not only specifically bind to ??T cell clone but also trigger the proliferation of ??T cell clone.Conclusion A healthy donor's ??T cell clone was successfully established,which laid a solid foundation for further study on ??T cells.

OBJECTIVE：To study the anti-asthmatic effects of artesunate on the durg-induced asthmat.METHOD:Drug-induced asthmatic model of guinea-pig. RESULTS:Using nubilous inhalation or intragastric administration,artesunate significantly inhibited the guinea-pig model asthma in a dose-dependent manner.It's ED50 were (99.95±7.69)mg.L-1(n=4) and (15.64±1.25)mg.kg-1.d-1(n=4) respectively. CONCLUSION:artesunate showed anti-athmatic effect in guinea-pig asthmatic model.

In order to promote rehabilitation exercises for breast cancer patients and to improve their quality of life,as well as to promote the development of nursing profession, our hospital has estab-lished a specialistnursing clinic for breast cancer since January 2016. We standardized nurses' qualification and training requirements, and performed management in patients'symptom assessment, nursingtechniqueguidance for breast cancer, sleep guidance, health education, long-term follow up, etc. After one year of implementation, symptom assessment was conducted 1283 times, rehabilitationexerciseguidance was 329 times, lymphatic drainage guidance was 297 times, sleep guidance was 159 times, health education was 591 times, sexual life with spouse guidance was 107 times, in-hospital nursing consultation was 48 times, family on-site demonstration was 29 times. The satis-faction rate was 98.4% which was higher than average level of the hospital according to outpatient investigation.

This article first introduces the current situation and existing problems of understanding the relation-ship between informed consent and doctor-patient communication in clinical practice,And then discusses from the ethical perspective, strengthen the positive role of the principle of informed consent.This paper under the principle of informed consent presented methods to improve the doctor-patient communication:to guide the doctor-patient communication informed consent, to achieve effective communication way of informed consent, strengthening the positive role of informed consent and some other suggestions.

Objective To analyze the clinical characteristics,therapy and follow-up of the patients with ulcerative colitis (UC).Methods Collect the date of 77 inpatients with UC at the Second Affiliated Hospital of Chongqing Medical University be-tween November,2009 and october,2014,and assigned the patients randomly.Results 69.9% of the patients who were in the ac-tivity stage were moderate severity.E2 (46.6%,34/73)and E3 (50.7%,37/73)were the common lesions range.The main clinical manifestations were abdominal pain,diarrhea,mucopurulent bloody stool and bloody stool.The enteroscopy mostly showed that in-testinal mucosa hyperemia,eddma,erosion,and small ulcers had the common features of UC.The common biopsy results were chro-nic inflammation and/or erosion.The average value of serum ALB decreases while the severity of UC patients increases.Drug thera-py was the main treatment of UC.The maintenance therapy was aminosalicylic acid,the effective ratio of treatment is 89.0%(55/73).Conclusion UC treatment plan basically follow the consensus and we should enhance the follow-up of UC patients.

Objective To investigate the clinical efficacy of electroacupuncture at Huatuo jiaji points plus acupuncture point injection in treating postherpetic neuralgia.Methods Sixty-two patients with postherpetic neuralgia were randomly allocated to a treatment group of 32 cases and a control group of 30 cases. The treatment group received electroacupuncture at Huatuo jiaji points plus acupuncture point injection and the control group, oral administration of carbamazepine tablets, vitamin B1 and vitamin B12. The pre-treatment and post-treatment clinical symptom score and VAS score were recorded in the two groups and the clinical therapeutic effects were compared between the two groups.Results The total efficacy rate was 93.8% in the treatment group and 66.7% in the control group; there was a statistically significant difference between the two groups (P<0.01). There were statistically significant pre-/post-treatment differences in clinical symptom item scores (pain, pruritus, burning sensation and sleep) in the two groups (P<0.05). There was a statistically significant pre-/post-treatment difference in the VAS score in the two groups (P<0.05). There was a statistically significant difference in pre-/post-treatment VAS score difference value between the treatment and control groups (P<0.05).Conclusion Electroacupuncture at Huatuo jiaji points plus acupuncture point injection is an effective way to treat postherpetic neuralgia.