Objective: To develop a type of oral biofilm to treat ora l ulcer.Methods:Chitosan film was prepared with chitosan only,comp osite chitosan film was prepared with chitosan(7 mg/cm 2),aloe (51 mg/cm 2),di caine(0.55 mg/cm 2) and EGF(40 IU/cm 2).Oral ulcer was made by local applicati on of crystal NaOH in 90 SD rats. The rats were divided into 3 groups with 30 in each. The oral ulcer in each group was treated by chitosan film(group C), compo site chitosan film(group CC) or without treatment(control). The ulcer healing wa s obsered by naked eye and examined pathologically. Results:The average healing time(d) of the ulcer in the groups of C,CC and control was 6.2 5 ? 1.29 ,5.58?1.3 and 8.75?2.49 respectively (C or CC vs Control P0.05). 2 days after treatment higher PCNA expression was observ ed in group CC than that in control(P

Objective:To assess the effect of basic fibroblast growth factor(bFGF) and chitosan(a water soluble derivation) on human periodontal ligament fibroblasts(HPDLFs). Methods:In vitro cultured HPDLFs of passage 5-7 were in culture medium only(group 1), or exposed to 10 ng/ml of bFGF(group 2), 10 ng/ml of bFGF combined with 0.2 mg/ml chitosan(group 3),10 ng/ml of bFGF combined with 2 mg/ml chitosan(group 4),0.2 mg/ml of chitosan(group 5) or 2 mg/ml of chitosan(group 6) for 5 days respectively. Cell proliferation was examined by MTT assay,alkaline phosphatase activity and osteocalcin synthesis were measured by AMP method and radioimmunoassay respectively.Results:Higher proliferation of HPDLFs was observed in group 2 and 3,higher alkaline phosphatase activity in group 5 and 6, and higher osteocalcin synthesis in group 3 and 4.Conclusions:bFGF combined with chitosan(0.2 mg/ml) may increase the proliferation of HPDLFs, stimulate HPDLFs to differentiate into osteoblasts.

This paper introduced a hand function rehabilitation system based on motor imagery (MI) brain-computer interface for hand function rehabilitation of stroke patients. The rehabilitation system contains three subsystems. Offline training subsystem displays the blank screen, a left or right hand movement video and arrow in turn, which respectively reminders patients to rest and make preparations for MI and instruct them how to do MI, and be doing MI. Finally, the patients' electroephalography (EEG) signals are acquired and processed togenerate a recognition model. Model update online training subsystem presents the black screen and a left or right arrow, the meanings ofwhich are the same as those in offline training subsystem. Then the acquired EEG signals are analyzed according to the established recognitionmodel. Next, the analysis result is derived to control the hand movement video to be played. The video can also act as a visual feedback,which makes patients' EEG signals easier to be recognized. The updated and more effective recognition model is built at last. Virtual reality(VR) online training subsystem constructs 3D grid models of VR scene, a 3D man model and its hand animations in the 3Dmax. Then, all ofthem are imported into Unity3D. The control methods of the animations are also designed in Unity3D. In the end, the patients' EEG signalsare analyzed according to the updated recognition model, thus controlling the hand movements of the 3D man in real time. The developedsystem has many characteristics, such as multilevel training and more immersion, which hopefully promotes the plasticity of central nervoussystem. The designed system provides new treatments for post-stroke hand function rehabilitation and further lays the foundation for family-mode rehabilitation.

CMV,HHV-6,HHV-7 and HHV-8 DNA in unstimulated whole saliva from 245 HIV-infected subjects and 30 healthy controls were examined by nested-PCR assays.Prevalence of CMV,HHV-6,HHV-7 and HHV-8 in saliva of HIV-infected subjects was 34.7%, 83.3%,70.2% and 14.3% respectively,that of the controls 10.0%,56.7%,70.0% and 0% respectively(between 2 groups,P <0.01).There was no difference of detection rates of the four HHVs in saliva between HIV patients with HAART(n =100)and non-HAART (n =145)(P >0.05).Multi-infection was observed in all subject.

Objective To assess oral health knowledge and behaviors of self-care among HIV infected patients in Yunnan, China. Methods Questionnaire survey was performed to collect the information about behaviors of oral self-care and seeking medical advice and diets of 216 HIV-infected patients who came from the Third Hospital of Kunming. The data were then statistically analyzed. Results Half of the patients were diagnosed over six months. The smoking of the HIV-positive were serious and the gingival bleeding was less, but there was obvious discomfort in oral cavity when eating. In addition,the standard method of cleaning teeth was below 20%, less of 50%patients could clean the oral cavity in time after diet and they didn't replace their toothbrushes timely. Over half of patients asked artificial tooth and exodontias on treatment. Conclusion It is necessary to pay more attention to the oral health knowledge and self-care behaviors of HIV-infected patients so as to improve the quality of life.

OBJECTIVETo assess the incidence of oral candidiasis and its influencing factors in patients with human immunodeficiency virus infection/acquired immunodeficiency syndrome (HIV/AIDS).

METHODSAn oral examination was conducted in the 1 566 HIV/AIDS patients in the Third Hospital of Kunming from March 2008 to September 2012 (M/F: 1 062/504, age range: 0.2 to 84.0 years old). The HIV viral load (HIV- RNA) and peripheral blood CD4 count were respectively analyzed by Bayer Q340 fluorescence signal surveying instrument (bDNA method) and flow cytometry analysis. The information on usage of highly active anti-retroviral (HAART) drugs and transmission of HIV were obtained through questionnaires. The incidence of oral candidiasis in patients with different HIV-RNA levels and CD4 count and the use of HAART was analyzed and compared.

RESULTSThe total incidence of oral candidosis was 31.0% (486/1 566) and there was no difference in sex. The oral lesions were presented by three types, psudomembranous candidosis (PC), erythematous candidosis (EC) and angular cheilitis (AC), and the morbidity was 13.9% (217/1 566), 17.0% (267/1 566) and 4.9% (77/1 566), respectively. The average level of CD4 count in psudomembranous candidosis, erythematous candidosis and angular cheilitis [81.0 (146.0), 74.0 (152.0) and 69.0 (121.5) cell/µl] showed no significant difference (P > 0.05). The incidence of oral candidiasis in non-HAART and HAART subjects were 36.3% (402/1 107) and 18.3% (84/459), respectively (P = 0.000). The CD4 count and absolute counts of HIV viral load in oral candidiasis patients and non-oral candidiasis patients had significant difference (Z = -10.261, P = 0.000 and Z = -4.762, P = 0.000).

CONCLUSIONSThe morbidity of oral candidiasis in HIV/AIDS patients in Yunnan Province was high, including PC, EC and AC and hyperplastic candidosis was not detected. The incidence was related to the degree of immune suppression and HIV viral load.

AIDS-Related Opportunistic Infections ; epidemiology ; Acquired Immunodeficiency Syndrome ; complications ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; CD4 Lymphocyte Count ; Candidiasis, Oral ; complications ; epidemiology ; Cheilitis ; Child ; Child, Preschool ; China ; epidemiology ; HIV Infections ; Humans ; Incidence ; Infant ; Middle Aged ; Mouth Diseases

OBJECTIVETo investigate the knowledge, attitude of the AIDS prevention and occupational safety in dentist in Kunming and west part of Yunnan and evaluate the effect of education and intervention.

METHOD165 dentists in Yunnan were tested by KABP questionnaire before education. All of the 165 dentists participated a course on AIDS prevention. After that course the same questionnaire was answered by participants.

RESULTThe correct rates obviously increased. The understanding rates of the oral prevention measure were obviously improved.

CONCLUSIONThe knowledge of AIDS prevention is very low in dentist in Kunming and west part of Yunnan. The education intervention can effectively improve the understanding level of the AIDS prevention in dentist.

Acquired Immunodeficiency Syndrome ; prevention & control ; transmission ; China ; Dentists ; Education, Dental ; methods ; Humans ; Infectious Disease Transmission, Patient-to-Professional ; prevention & control ; Occupational Diseases ; prevention & control ; Occupational Health ; Surveys and Questionnaires

OBJECTIVETo determine the incidence of human herpes virus (HHV) 1-4 type including herpes simplex virus type-1 (HSV-1), herpes simplex virus type-2 (HSV-2), varicella-zoster virus (VZV), Epstein-Barr virus(EBV) in the saliva of human immunodeficiency virus (HIV) -infected patients.

METHODSThe incidence of salivary HSV-1, HSV-2, VZV and EBV from 245 HIV-seropositive individuals and control group was used to investigate by polymerase chain reaction(PCR) or nested PCR. The data was analyzed by SPSS 18.0 statistical software.

RESULTSIn the 245 HIV-seropositive individuals, the detection rates of HSV-1, HSV-2, VZV, EBV were 29.0%, 3.3%, 4.1%, 82.0%. In the control group, the detection rates of HSV-1, HSV-2, VZV, EBV were 13.3%, 0, 0, 36.7%. Four HHVs were significantly more prevalent in the salivas of HIV-seropositive persons than those in the control group (P < 0.01). The detection rates of HSV-1, HSV-2, VZV and EBV DNA were no difference between the HIV-positive group with highly active antiretroviral therapy (HAART) and HIV-positive group without HAART (P > 0.05).

CONCLUSIONThere is a high prevalence of HHV infection in HIV-infected people in Yunnan. The most common virus are EBV, followed by HSV-1, but VZV and HSV-2 are rarely detected. HHV co-infection is also observed.

Adult ; China ; DNA, Viral ; HIV Infections ; Herpesvirus 3, Human ; Humans ; Incidence ; Polymerase Chain Reaction ; Prevalence ; Saliva

Objective To compare the discrepancies among results of three commonly used laboratory direct test methods for maximal oxygen uptake(VO2max),explore their linear regression relationships,mutual predictability and comparability.Methods Using a quasi-experimental design of cluster sampling and within-group interaction design,20 male cross-country skiers were tested for VO2max using the Bruce protocol(Method 1),90-second incremental load exercise on power bicycle(Method 2),and 1-minute incremental load exercise on treadmill(Method 3),with an interval of one week.The indepen-dent and dependent variable were the three VO2max test methods and the VO2max,respectively.Results Significant differences were found in the average VO2max of the three test results,with the value mea-sured by Method 1 ranking the first,followed by that assessed by Method 3 and Method 2(P<0.05).Moreover,the frequency of individual differences in the results of the three methods showed that the VO2max of Method 1 was about 6 and 3 ml/min·kg higher than that measured by Method 2 and 3.However,at the same treadmill speed,the average blood lactate evaluated using Method 3 was higher than Method 1,and the speed reached aerobic and anaerobic thresholds about one speed unit(1 km/h)lower than Method 1.Meanwhile,linear regression analyses of the test results between Method 1 and 2,as well as Method 1 and 3 showed that both the regression models and coefficients were statis-tically significant(P<0.001),with the R-squared values of 9.25 and 9.05,respectively.Conclusion The Bruce protocol performs best in assessing the maximum value of the athlete's VO2max phase,whose results have linear regression relationships with the other two methods,and can be used for pre-dicting their results.Moreover,athletes of different events and levels can choose different VO2max test methods accordingly.Lastly,the speed and heart rate ranges corresponding to the aerobic and anaero-bic thresholds can serve as an effective and convenient method to control the training intensity.

Objective: To investigate the characteristics of oral lesions in HIV infected patients aged 50 years or older in Yunnan.Methods: 315 HIV infected patients aged 50 years and older from January 2013 to December 2016 were included in a cross-sectional survey at the Third People's Hospital of Kunming. The characteristics of oral mucosal lesions and the relationship between the oral lesions and CD4 count level were analyzed. Results: 13 kinds of oral mucosal lesion in 185 out of the 315 cases (58. 73％) were observed. 122 cases were with candidiasis (38. 73％), including 68 (21. 59％) of pseudomembranous type, 52 (16. 51％) of erythema and 18 (5. 71％) of stomatitis; 25 (7. 94％) were with Hairy leukoplakia, 20 (6. 35％) with recurrent oral ulcer, 17 (5. 40％) with fissured tongue, 11 (3. 49％) with herpes simplex. 2 and 3 lesions were detected in 41 cases (13. 02％) and 1 case (0. 32％), respectively. The rate of simultaneous detection of more than 2 lesions and the detection rate of oral candidiasis were related to the CD4 count level. Conclusion: Oral mucosal lesions are common in elderly patients with HIV infection, and oral candidiasis is the main type.