Objective: To develop an assay for the determination of cinnamaldehyde in Cortex Cinnamomi and Shentai Plaster. Methods: Hypersil C 18 column (200?4.6mm, 5?m) was used. The mobile phase consisted of a mixture of methanol acetonitrile water tetrahydrofuran (25∶15∶55∶5) with a flow rate of 0.8mL?min -1 . The detection wavelength was at 285 nm. Results: The calibration curve was linear in the range of 32～320ng for cinnamaldehyde ( r =0.9995). The average recoveries of cinnamaldehyde in Cortex Cinnamomi and Shentai Plaster were 97.8% (RSD=3.0%) and 92.2% (RSD=2.9%), respectively. Conclusion: This HPLC method is simple, rapid and accurate, and it can be used in the control of product quality and preparation process.

Object To study the HPLC fingerprints and establish a sensitive and specific method for controlling the quality of Radix Paeoniae Rubra (RPR) Methods The HPLC method was applied for quality assessment of RPR All 18 samples collected from different places were determined A Hypersil C 18 column (200 mm?4 6 mm, 5 ?m) was used with the mobile phase being acetonitril 0 025 mol/L H 3PO 4: THF (9 5∶90 5∶1 25), flow rate being 0 8 mL/min, detecting wavelength being 254 nm, the column temperature being at room temperture Results This method had a good repeatability and reproducibility, and the ratio of peaks' area of different habitat samples were different Conclusion The method is suitable to differentiate RPR form different sources conveniently, and can be used as a quality control method for this herb

AIM: To develop an RP HPLC method for determination of mangiferin and glycyrrhizic acid in Suanzaoren Decoction(Semen Ziziphi Spinosae, Poria, Rhizoma Ligustici Chuanxiong, Rhizoma Anemarrhenae, Radix Glycyrrhizae). METHODS: Hypersil C 18 column was used. The mobile phase consisted of acetonitrile water(13∶87,v/v) with 1% glacial acetic acid for mangiferin and methanol acetonitrile water(25∶15∶60,v/v) with 1% acetic acid for glycyrrhizic acid, respectively. Detection wavelengths were set at 320nm and 254nm, respectively. The internal standards were vanillin for mangiferin and mebendazole for glycyrrhizic acid, respectively. The flow rates were both 0.8mL?min -1 . RESULTS: The linear ranges for mangiferin and glycyrrhizic acid were 10.72～85.76?g?mL -1 ( r =0.9998) and 0.080～0.80mg?mL -1 ( r =0.9997), respectively. The average recoveries were 97.2% with RSD 3.0% and 97.6% with RSD 2.4%, respectively. CONCLUSION: This method is simple, rapid and accurate, and can be used for the determination of mangiferin and glycyrrhizic acid in Suanzaoren decoction.

Object To develop an RP-HPLC method for determination of spinosin and ferulic acid in Suanzaoren Decoction (SZRD). Methods Hypersil C 18 column was used. The mobile phase consisted of acetonitrile-water with 1% glacial acetic acid (17∶83) for spinosin and (16∶84) for ferulic acid. Detection wavelengths were set at 334 and 320 nm, respectively. The internal standard was p-hydroxybenzaldehyde for ferulic acid. Results The linear ranges for spinosin and ferulic acid were 0.960—13.44 ?g/mL (r=0.999 8) and 2.04—27.2 ?g/mL (r=0.999 7), respectively. The recoveries for them were 98.4% and 98.8%, respectively. The RSD were 2.5% and 2.8%, respectively. Conclusion This method is simple, rapids and accurate, and can be used for the determination of spinosin and ferulic acid in SZRD.

Objective: To establish a RP HPLC for determination of puerarin in Heat clearing Toxin resolving Decoction. Methods: A Hypersil C 18 column with acetonitrile 2% glacial acetic acid (8.5:91.5) as a mobile phase was used. The flow rate was 0.8ml?min 1 and the detective wavelength was 250nm. Results: The calibration curve was linear at a range of 0.424 4.240?g for the puerarin (r=0.9998). The average recovery was 101.3% and the relative standard deviation (RSD) was 1.4%. Conclusion: The methld is simple, accurate with a good reproducibility and can be used as a quantitative analysis method for this preparation.

Object To study the pharmacokinetics of safflower yellow A, a major ingredient in the crude drug of Carthamus tinctorius L., in rats in vivo. Methods RP HPLC method was set up used to determine the content of safflower yellow A in plasma of rat. The chromatographic condition included Hypersil ODS2 column (200 mm?4.6 mm, 5 ?m); the mobile phase consisting of methanol 0.2% acetic acid (24∶76); the flow rate at 0.8 mL/min; the detective wavelength at 410 nm. Riboflavin was used as internal standard. Safflower yellow A was injected into the rats through caudal vein, and its concentrations at different time were determined in healthy rat after 24 h fasting. The data were processed with the software 3P87. Results The primary pharmacokinetic parameters were: V c (0 30?0 04) L/kg, CL (1.12?0.33) mL/min, K e (0.91?0.19) h -1 , t 1/2 (0 76?0 10) h, AUC (24.97?4.83) (?g?h)/mL, respectively in iv safflower yellow A to the rats. Conclusion The safflower yellow A in rats is fited to one compartment model, and it is distributed and eliminated quickly.

AIM: To establish a novel method for the overall quality control of Radix et rhizoma seu caulis Acanthopanacis senticosi on the ground of HPLC digitized fingerprint.METHODS: The chromatographic fingerprints were obtained by injecting 10 ?L of the sample solution each time on a Century SIL BDS column(20 cm?4.6 mm,5 ?m) with the gradient elution solvent system composed of 1% acetate acid water and 1% acetate acid acetonitrile.The flow rate was 1 mL/min,the colunm temperature was maintained at(30?0.15)℃ and the detection wavelength was set at 265 nm. RESULTS: 31 co-possessing peaks were selected as the fingerprint peaks of Radix et rhizoma seu caulis Acanthopanacis senticosi and the similarities between the chromatographic fingerprints and the herbal drugs were calculated taking chlorogenic acid peak as the reference peak.The chromatographic fingerprints also were evaluated by the chromatographic fingerprint index(F),the relative index(Fr). CONCLUSION: This method with good precision and reproducibility can be useful for the quality control of Radix et rhizoma seu caulis Acanthopanacis senticosi.

OBJECTIVE: To compare the dissolution rate between Sanhuang Rapid-disintegrate tablets and Sanhuang sugar-coated tablets. METHODS: The dissolution rates of two preparations of Sanhuang tablets were determined with baicalin as index in accordance with the dissolution determination method stated in China Pharmacopeia, with determination results subjected to statistical treatment. RESULTS: Significant differences were noted in the comparison of dissolution parameter between the two dosage forms. In aqueous medium, the cumulative dissolution rate of Sanhuang sugar-coated tablets at 45 min was 43%～73%, whereas that of the Rapid-disintegrate was above 90%. CONCLUSION: The dissolution rate of Sanhuang Rapid-disintegrate tablets is higher than that of Sanhuang sugar-coated tablets.

Object To develop the analysis method to determine the content of cannabidiol in the hemp seed oil by HPLC. Methods The chromatographic condition was Irregular-H-C 18 column (250 mm? 4.6 mm, 10 ?m). A mixture of methanol-acetonitrile-water-acetic acid (25∶50∶25∶0.4) was used as the mobile phase with a flow rate of 0.8 mL/min and the detection wavelength was 220 nm at room temperature. Results The calibration curve for cannabidiol showed good linear correlation within the concentration range of 1.2 — 9.6 ?g/mL (r=0.999 4). The average recovery and RSD was 94.6% and 1.9% (n=9) respectively. Conclusion The method is convenient, reliable and with good reappearance.