1.Levels of sTNFR、 TNF? in Serum and PBMC Culture Medium Supernatants of Systemic Lupus Erythematosus
Kaiming ZHANG ; Xiaoying YANG ; Xingping YIN
Chinese Journal of Dermatology 1995;0(01):-
Objective To study the levels of soluble tumor necrosis factor receptor (sTNFR)、 tumor necrosis factor alpha(TNF? ) in systemic lupus erythematosus(SLE) patients and its clinical significance. Methods Double antibody sandwich ELISA was used to determine soluble tumor necrosis factor receptor typeⅠ (sTNFRⅠ )、 soluble tumor necrosis factor receptor typeⅡ (sTNFRⅡ ) and TNF? levels in patient serum and peripheral blood mononuclear cell(PBMC) culture supernatants. Other immunological tests were carried out simultaneously. Results① The serum sTNFRⅠ、 sTNFRⅡ、 TNF? sTNFRⅠ /TNF? and sTNFRⅡ /TNF? ratios were significantly higher in SLE patients than those in normal control group(P
2.Puerarin reduces ethanol-induced-apoptosis of spermatogenic cells in rat testis
Hongyun HE ; Yihao DENG ; Xinwen YANG ; Kaiming YANG
Basic & Clinical Medicine 2006;0(12):-
Objective To investigate potential protection by protect against ethanol-induced apoptosis of spermatogenic cells in rat testis.Methods Thirty SD adult male rats were randomly divided into three groups: normal group,alcohol group and puerarin group.At 40th day,BCL-2 and BAX of spermatogenic cells of testis tissue were checked by RT-PCR and immunohistochemistry;Apoptosis of spermatogenic cells was determined by TUNEL.Results The results of RT-PCR and immunohistochemistry indicated that BCL-2 and BAX of spermatogenic cells were not significanty different between puerarin group and normal group,but there was the significant difference between alcohol group and puerarin group(P
3.Puerarin reduces ethanol-induced-apoptosis of spermatogenic cells in rat testis
Hongyun HE ; Yihao DENG ; Xinwen YANG ; Kaiming YANG
Basic & Clinical Medicine 2009;29(12):1291-1295
Objective To investigate potential protection by protect against ethanol-induced apoptosis of spermato-genie cells in rat testis. Methods Thirty SD adult male rats were randomly divided into three groups: normal group, alcohol group and puerarin group. At 40th day, BCL-2 and BAX of spermatogenic cells of testis tissue were checked by RT-PCR and immunohistochemistry; Apoptosis of spermatogenic cells was determined by TUNEL. Re-suits The results of RT-PCR and immunohistochemistry indicated that BCL-2 and BAX of spermatogenie cells were not significanty different between puerarin group and normal group, but there was the significant difference between alcohol group and puerarin group (P <0.01). Apoptosis of spermatogenic cells in alcohol group was significantly higher than normal group. Conclusion Spermatogenic cells could generate apoptosis by changing the expression of BCL-2 and BAX. Puerarin could inhibit this damage of didymus by alcohol.
4.Apoptosis in Adult Mouse Brain after Chronic Poisoning of Ketamine
Ju YANG ; Xiaojing LI ; Zhixiang ZHANG ; Kaiming LU ; Shizhong BIAN
Journal of Forensic Medicine 2013;(5):325-329
Objective To study the effect of chronic poisoning of ketamine on brain cell apoptosis in adult mouse under different duration and doses. Methods The mouse model of chronic poisoning of ketamine was established on adult mouse by tail vein injection of ketamine twice every week with different doses (4, 10, 20 and 30 m g/kg). The mice were sacrificed after continuous injection of ketamine of 1, 2, 4, 8 and 12 weeks. The qualitative assessment of apoptosis was made by transmission electron microscope and the quantitative assessment was made by Caspase-3 im m umofluorescence staining method and terminal deoxynucleotidyl transferase-mediated dU TP nick end labeling (TUNEL ) to estimate the time point of apoptosis. All the experimental results were statistically analyzed. Results The neuron apoptosis was ob-served in hippocam pus and corpus striatum by transmission electron microscope one week after adminis-tration, and continued for eight weeks. High level of Caspase-3 expression was observed one week after administration, but with a lowlevel expression after 4 weeks. The num ber of TUNEL positive cells ob-viously increased one week after administration and maintained in ahigh num ber at 4 weeks. Conclu-sion Ketamine by tail vein injection could induce neuron apoptosis in adult mouse.
5.Percutaneous kyphoplasty for treatment of osteoporotic vertebral body compression fractures in the elderly
Zhiyong YANG ; Kaiming WANG ; Bingjun LEI ; Qing ZHANG
Chinese Journal of Geriatrics 2017;36(7):788-790
Objectives To study the clinical effects of percutaneous kyphoplasty on senile osteoporotic vertebral body compression fractures.Methods The 87 elderly patients with osteoporotic vertebral body compression fractures were randomly grouped into control group (n=42) treated with percutaneous vertebroplasty and observation group (n=45) treated with percutaneous kyphoplasty in our hospital from June 2013-March 2016.And the clinical curative effect before and after treatment was compared and analyzed.Results The visual analog scale (VAS) pain score in observation group versus control group was (8.49±2.86) vs.(8.56±2.98),(t=0.11,P>0.05) before treatment,and[(2.58±1.34) vs.(3.34±2.01),t=2.09,P< 0.05],[(2.06±0.97) vs (3.87±1.96),t=5.51,P<0.05]and[(1.09±0.89) vs (2.37±1.29)],t=5.42,P<0.05]at 1 week,4 weeks,and 12 weeks after treatment respectively,showing statistically significant improvement after treatment.The data of kyphosis angle by using Cobb angle,Oswestry lumbar dysfunction index (ODI),mean time of postoperative ambulation,and mean postoperative hospital stay were similar to that of the visual analog scale (VAS) pain score,showing statistically significant improvement after treatment (all P<0.05).The incidence[cases (%)]of bone cement leakage was lower in observation group[3(6.7)]than in control[9(21.4)](χ2=3.98,P<0.05).Conclusions Clinical treatment effects of percutaneous kyphoplasty on the elderly patients with osteoporotic vertebral body compression fractures are superior to that of percutaneous vertebroplasty.Percutaneous kyphoplasty can effectively relieve pain,and improve body function disorders and the quality of life.
6.The effects of different modes of mechanical ventilation under general anesthesia on children undergoing removal of foreign body with bronchofiberscopy
Kaiming FAN ; Zhenwei WAN ; Changke LI ; Yinlou YANG ; Bing CHEN
Journal of Chinese Physician 2015;17(7):1009-1012
Objective To explore the effects of different ventilation modes of tracheobronchial foreign body in children with fiberoptic operation under general anesthesia.Methods Sixty children (1ys≤ age≤3ys) undergoing fiberoptic bronchoscopy tracheal foreign body removal according to the combinations of different ventilation modes during and after fiberoptic bronchoscopy (FOB) procedures were divided into group A [volume control ventilation (VCV) + VCV,n =20],group B [pressure control ventilation (PCV) + VCV,n =20] and group C (PCV + PCV,n =20) randomly.The P mean,Pmax,and PetCO2 during and after fiberoptic bronchoscopy procedures were monitored.The SpO2,PaO2,and PaCO2 after mechanical ventilation 1.5 hours were recorded.Results Compared to group A,groups B and C had lower P max and P mean (P < 0.05) during the FOB procedures.Compared to groups A and B,group C had a lower P max and P mean (P <0.01) after the FOB procedures.At the 1.5 hours after the procedure,all the children showed significant increase in SpO2 and PaO2 (P < 0.05) and decrease in PaCO2 (P < 0.05) in groups A,B,and C.Conclusions When fiberoptic bronchoscopy in tracheobronchial foreign body operation is applied in children undergoing general anesthesia,the pressure control ventilation (VCV) mode can decrease the pressure of airway (Paw) and PaCO2 than volume control mode during procedure.
7.Diminished capacity of mesenchymal stem cells in lesions of patients with psoriasis to inhibit T cell proliferation
Ruifeng LIU ; Xincheng ZHAO ; Yuanwen YANG ; Kaiming ZHANG
Chinese Journal of Dermatology 2013;46(11):779-783
Objective To evaluate the inhibitory effect of mesenchymal stem cells (MSCs) in lesions of patients with psoriasis on T lymphocyte proliferation.Methods Tissue specimens were obtained from the lesions of 15 patients with psoriasis vulgaris (7 at progressive stage and 8 at resting stage) and normal skin of 15 human controls from the Department of Urology and Plastic Surgery,Taiyuan City Centre Hospital.MSCs were isolated from these skin specimens,cultured,and identified using flow cytometry and in vitro differentiation assay.Enzyme-linked immunosorbent assay (ELISA) was performed to detect the concentration of interleukin (IL)-6,IL-1 1,hepatocyte growth factor (HGF) and transforming growth factor (TGF)-β1 in the culture supernatant of third-passage MSCs.Peripheral blood T cells were obtained from a healthy adult and cocultured with the third-passage MSCs for four days.Then,cells were counted and methyl thiazolyl tetrazolium (MTT) assay was conducted to evaluate the proliferation of T cells.One-way analysis of variance (ANOVA) and Student-Newman-Keuls (SNK) test were carried out to compare the proliferation of T lymphocytes,and two independent samples t test to compare the concentrations of cytokines.Results Inverted microscopy revealed that the patient-and control-derived MSCs shared similar morphological properties and multi-directional differentiation capacity,along with the expression of CD29,CD44,CD73,CD90 and CD105,but absence of CD34,CD45 and HLA-DR on cell surface.After coculture with MSCs from the patients and controls for four days,the count of T lymphocytes per milliliter was (1.67 ± 0.34) × 105 and (1.04 ± 0.29) × 105 respectively (P< 0.01),and the proliferative activity (expressed as absorbence at 492 nm)was 0.317 ± 0.021 and 0.275 ± 0.007 respectively (P < 0.01).Compared with the control-derived MSCs,the patient-derived MSCs showed a significantly higher level of IL-1 1 ((181.37 ± 31.74) vs.(130.07 ± 29.20) ng/L,t =5.32,P < 0.01),but a lower level of lL-6 ((61.67±17.53) vs.(76.74±18.96) ng/L,t=2.61,P<0.05)and HGF ((319.24 ± 41.03) vs.(352.35 ± 51.47) ng/L,t =2.25,P< 0.05),as well as a similar level of TFG-β1,in the culture supernatant.Conclusions The inhibitory effect of MSCs in psoriatic lesions on T lymphocyte proliferation is diminished,which may contribute to the pathogenesis of psoriasis.
8.Effects of dexmedetomidine on postoperative cognitive function and monocytes Toll-like receptor 2 and Tolllike receptor 4 expression in elderly patients
Chengxuan QUAN ; Xiaoyu YANG ; Minghua CHEN ; Zhonghua HU ; Kaiming DUAN ; Qin LIAO ; Wen OUYANG
Chinese Journal of Anesthesiology 2011;31(11):1331-1334
ObjectiveTo investigate the effects of dexmedetomidine on postoperative cognitive function and monocytes Toll-like receptor 2 (TLR2)and TLR 4 expression in elderly patients.MethodsForty-five ASA Ⅰ or Ⅱ elderly patients aged ≥65 yr weighing 53-72 kg were randomly divided into 3 groups: control group (group Ⅰ ) and different doses of dexmedetomidine groups(groups Ⅱ and Ⅲ ).Dexmedetomidine 1.0 μg/kg was injected iv over 15 min after anesthesia induction,and then was infused at a rate of 0.5 μg·kg-1 ·h-1 (group Ⅱ ) or 1.0 μg· kg-1 ·h-1 (group Ⅲ ) untile the end of operation.Group Ⅰ received equal volume of normal saline.Blood samples were taken before anesthesia induction,at 1.5 h after the beginning of operation,at the end of operation and at 24 h after operation(T,-T5 ) for determination of monocytes TLR2 and TLR4 expression by flow cytometrybased method.Postoperative cognitive function was evaluated at 1 d before and 7 d after operation with Mini-mental state examination and Wechsler memory scale and Wechsler adult intelligence scale,and the postoperative cognitive dysfunction was recorded.ResultsThe incidence of postoperative cognitive dysfunction and monocytes TLR2 and TLR4 expression were significantly lower in groups Ⅱ and Ⅲ than in group Ⅰ,and in group Ⅲ than in group Ⅱ (P < 0.05).ConclusionDexmedetomidine can prevent postoperative cognitive dysfunction in elderly patients,and the mechanism may be related to down-regulation of monocytes TLR2 and TLR4 expression.
9.Correlations between BDNF genetic polymorphism and postpartum depression in cesarean section parturient
Yingyong ZHOU ; Saiying WANG ; Mi YANG ; Zhendong HUANG ; Jiahui MA ; Kaiming DUAN
Medical Journal of Chinese People's Liberation Army 2017;42(6):538-544
Objective To study the correlations between the genetic polymorphism of brain-derived neurotrophic factor (BDNF) and the postpartum depression (PPD) in cesarean section parturient. Methods Three hundred and sixty parturients, who underwent cesarean section under spinal anesthesia from Feb. 2014 to Feb. 2015 in Third Xiangya Hospital of Central South University or Hunan Maternal and Child Health Hospital, were selected as subjects. The general information of parturients was recorded and Edinburgh Postnatal Depression Scale (EPDS) was used to evaluate the depression condition of parturients at the prenatal 1 day and the 42th day postpartum, and with a cut-off point of 12/13 for identifying PPD. The genotypes of BDNF gene locus G712A, rs56164415, rs11030100, rs11030101 and rs6265 were measured by Sequenom? Mass Array SNP. Finally, the correlations of PPD to different genotypes and general information of parturients were statistically analyzed. Results The incidence of PPD among the selected subjects was 7.2%. Pregnancy mental stress, poor pregnancy mood, perinatal elevated monocyte count, prenatal depression mood and BDNF gene locus rs6265 mutation all could affect the incidence of PPD in cesarean section parturients (P<0.05). No statistically significant difference existed between BDNF gene G712A, rs11030101, rs11030100 and rs56164415 locus mutation and PPD (P>0.05), and their haploid forms were not related to PPD also. Conclusion BDNF rs6265CC genotype, pregnancy mental stress, poor pregnancy mood, perinatal elevated monocyte count and prenatal depression mood are the risk factors for postpartum depression.
10.Aberrant expression of circular RNA in mesenchymal stem cells from psoriatic skin lesions
Ruifeng LIU ; Xiaohong YANG ; Jiannan LIANG ; Kaiming ZHANG
Chinese Journal of Dermatology 2018;51(10):723-728
Objective To investigate the role of circular RNA (circRNA) in the occurrence of psoriasis.Methods Mesenchymal stem cells were isolated from skin lesions of 15 patients with psoriasis (psoriasis group) and skin tissues of 15 healthy human controls (control group) separately,and then subjected to cultivation.Flow cytometry and cell differentiation assay were performed to identify these mesenchymal stem cells.RNA sequencing was conducted to measure the expression of circRNA,and detailed bioinformatics analysis was performed.Then,7 differentially expressed circRNAs were selected,and a circRNA-microRNA interaction network was established.In this network,3 related microRNAs were selected and validated by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR).Statistical analysis was carried out by a two-sample t-test for comparison of qRT-PCR results between the psoriasis group and control group.Results As RNA sequencing showed,a total of 6 323 circRNAs were identified,and 3 227 out of these circRNAs were first reported in this study.Compared with the control group,129 circRNAs were differentially expressed in the psoriasis group,including 123 up-regulated circRNAs and 6 down-regulated circRNAs.The predicted circRNA-microRNA interaction network showed that several psoriasis-related microRNAs were associated with the 7 circRNAs,such as miR-17-5p,miR-30e-5p,miR-142-3p/5p,miR-369-3p,miR-184,miR-4490,miR-654-3p and miR-423-5p.qRT-PCR also confirmed that the 7 differentially expressed circRNAs were up-regulated in the psoriasis group.Compared with the control group,the expression of the 3 selected microRNAs significantly decreased in the psoriasis group (t =3.993,3.217,2.918,respectively,all P < 0.05).Conclusion There is aberrant expression of circular RNAs in the mesenchymal stem cells from psoriatic skin lesions,which may take part in the occurrence of psoriasis.