Objective To investigate the therapeutic mechanism of Shengjiang Powder(SP)on acute lung injury.Methods Forty healthy SD male rats were divided into 3 groups randomly: normal group,model group,and SP group.After administration for 6 days,acute lung injury rat models were established by injecting lipopolysaccharide(LPS)into sublingual vein.Lung histological sections were prepared for the detection of NF-?B expression by immmunohistochemistry.The images of the lung histological sections were captured and the average gray values of nucleus of endothelial cells in lung micrangium were measured.Results Compared with the model group,SP could depress the expression of NF-?B in the nucleus of lung microvascular endothelial cells,and statistical difference existed between the two groups(P

OBJECTIVETo study the relationship between donor TNFalpha - 308 (G/A) genotype and recipient acute graft versus host disease (aGVHD).

METHODSTNFalpha - 308 (G/A) and TNFalpha - 308 (G/G) genotypes were analyzed by DHPLC and DNA sequence in twenty-one degree III/IV (III/IV) aGVHD patients, and twenty-eight degree 0/I (0/I) aGVHD patients. Serum TNFalpha levels were determined by ELISA.

RESULTSThe frequency of TNFalpha - 308 (G/A) genotype was significantly higher in III/IV aGVHD patients than in 0/I aGVHD patients (8/21 vs 1/28) (P < 0.05); and so did for higher serum TNFalpha levels (P < 0.05). TNFalpha levels were higher in III/IV aGVHD patients than in 0/I aGVHD patients in TNFalpha - 308 (G/G) patients (P < 0.01).

CONCLUSIONTNFalpha - 308 (G/A) is positively related to serum TNFalpha levels and most likely contributes to high risk for III/IV aGVHD.

Acute Disease ; Base Sequence ; Blood Donors ; Chromatography, High Pressure Liquid ; methods ; DNA ; chemistry ; genetics ; Enzyme-Linked Immunosorbent Assay ; Gene Frequency ; Genotype ; Graft vs Host Disease ; blood ; genetics ; Hematopoietic Stem Cell Transplantation ; Humans ; Molecular Sequence Data ; Sequence Analysis, DNA ; Tumor Necrosis Factor-alpha ; genetics ; metabolism

Objective:To evaluate the association between RUNX3 expression and Helicobacter pylori(H.pylori)-associated precancerous gastric lesions.Methods: A population-based study was conducted in Linqu County,Shandong Province,a high-risk area of gastric cancer in China.RUNX3 expression was determined by immunohistochemistry analysis in 1 026 H.pylori infected subjects with different gastric lesions.Results: Among 1 026 subjects,359(35.0%,359/1 026) was positive.The prevalence rates of RUNX3 expression decreased steadily with severity of gastric lesions,65.6%(40/61) among those with superficial gastritis /normal(SG/N),and 22.4%(60/268) among those with dysplasia(DYS)(P

PURPOSE: This study is intended to investigate the effects of plants or plant-derived antioxidants on prevention of osteoporosis through the maintenance of reactive oxygen species (ROS) at a favorable level. MATERIALS AND METHODS: In this study, a novel antioxidant, namely 3,4,5-Trihydroxy-N-[4-(5-hydroxy-6-methoxy-pyrimidin-4-ylsulfamoyl)-phenyl]-benzamide (ZXHA-TC) was synthesized from gallic acid and sulfadimoxine. Its effect on osteoblast metabolism was investigated via the detection of cell proliferation, cell viability, production of ROS, and expression of osteogenic-specific genes including runt-related transcription factor 2 (RUNX2), bone sialoprotein (BSP), osteocalcin (OCN), alpha-1 type I collagen (COL1A1), and osteogenic-related proteins after treatment for 2, 4, and 6 days respectively. RESULTS: The results showed that ZXHA-TC has a stimulating effect on the proliferation and osteogenic differentiation of primary osteoblasts by promoting cell proliferation, cell viability, and the expression of genes BSP and OCN. Productions of bone matrix and mineralization were also increased by ZXHA-TC treatment as a result of up-regulation of COL1A1 and alkaline phosphatase (ALP) at the early stage and down-regulation of both genes subsequently. A range of 6.25x10(-3) microg/mL to 6.25x10(-1) microg/mL is the recommended dose for ZXHA-TC, within which 6.25x10(-2) microg/mL showed the best performance. CONCLUSION: This study may hold promise for the development of a novel agent for the treatment of osteoporosis.
Alkaline Phosphatase/metabolism ; Bone Morphogenetic Proteins/pharmacology ; Cell Differentiation/*drug effects ; Cell Proliferation/*drug effects ; Collagen Type I/genetics ; Core Binding Factor Alpha 1 Subunit ; Down-Regulation ; Gallic Acid ; Osteoblasts/*drug effects ; Osteocalcin/metabolism ; Osteogenesis/drug effects ; Osteoporosis/*prevention & control ; Reactive Oxygen Species ; Up-Regulation

Objective To understand the application and research progress of Mendelian randomization (MR) studies related to gastric cancer and provide a scientific basis for gastric cancer prevention. Methods Published studies on risk factors of gastric cancer based on MR methods were searched in PubMed, Web of Science, EMBASE, CNKI, and WANFANG DATA from the establishment of each database to November 1^9th, 2022. Two researchers examined the eligibility of studies, extracted key information, and assessed the research quality independently. Results A total of 30 publications published from 2016 to 2022 were included in the study, and 20 were judged to be of high quality. These studies examined the relationship between behaviors and lifestyle factors, anthropometric characteristics, indicators of biological exposure, and other pathological conditions and gastric cancer, and the results suggest potential causal associations between smoking and other factors and the risk of gastric cancer. Conclusion Previous MR studies extensively investigated the causal association between internal and external exposures or traits and gastric cancer and provided positive evidence of gastric cancer etiology. However, MR studies may be subject to methodological limitations. Interpretation of results needs to be approached with caution, which necessitates the integration with biological plausibility and evidence from observation studies.

Objective To deepen the understanding of curative effect and adverse drug reaction of brentuximab vedotin in the treatment of Hodgkin's lymphoma.Methods One case of newly diagnosed Hodgkin's lymphoma who treated with brentuximab vedotin was collected.The clinical features,laboratory examination,treatment procedure,prognosis were analyzed,and the relevant literature was reviewed.Results The patient was twenty years old,female,found the right neck masses for five days,diagnosed with Hodgkin's lymphoma,mixed cell type,stage Ⅳ group B.Chose the chemotherapy regimen BV + GABVD for 4 courses,the PET-CT indicated CR,then used GABVD 4 courses,now in the stage of clinical observation and followed up.Conclusion BV is a kind of antibody-drug conjugate which targeted on CD30 protein.The FDA approved for the treatment of Hodgkin's lymphoma and systemic anaplastic large cell lymphoma.The common adverse reactions are granulocyte,peripheral neuropathy,fatigue,nausea and vomiting.BV can significantly improve the prognosis of CD30 positive Hodgkin's lymphoma,but need to pay attention to the prevention of adverse reactions.

Objective:To analyze some problems identified in the transformation of scientific and technological achievements in hospitals and put forward corresponding strategies in order to provide reference for other hospitals.Methods:This paper analyzes some problems identified in the transformation of scientific and technological achievements in hospitals.Results:The common problems identified in the research include: too many steps in the transformation of scientific and technological achievements, market information is asymmetry, there is also risks of leakage and infringement, lack of professional institutions and specialized talents. Several strategies to solve these problems were proposed.Conclusions:The management department needs to create a good external environment to adapt to the transformation of achievements. That is to integrate the intellectual property layout and the transformation of achievements; optimize the transformation model; implement the whole process of security supervision to protect the confidentiality; improve the construction of transformation platform; strengthen the construction of professional talent team. This can promote the "bench-to-bed" transition of medical scientific research, as well as assure the long-term development of scientific and technological innovation.

Objective:Reflux esophagitis (RE) may be negatively correlated with Helicobacter pylori ( H. pylori) infection, but the conclusion and relevant mechanism is still controversial. This study proposed to explore the correlation between RE and H. pylori infection based on natural population. Methods:From July 2013 to December 2014, 3 940 residents aged 40-69 years were recruited in Linqu County of Shandong Province and Hua County of Henan Province by the whole sampling method. All the subjects underwent gastroscopy, and gastric mucosa biopsy specimens were collected for pathological diagnosis and Warthin-Starry (WS) staining to identify H. pylori infection. Venous blood samples of some subjects were collected for H. pylori immunoglobulin G ( H. pylori-IgG) detection. Also, demographic and sociological data were collected. Chi-square test and logistic regression were used to analyze the correlation between RE and H. pylori infection. Results:A total of 359 cases of RE were detected. Excluding RE and other upper gastrointestinal organic diseases, 3 382 cases were considered as controls. Chi-square test showed that WS staining positive rate in RE group was significantly lower than that in control group ( P=0.023), but there was no significant difference in the positive rate of H. pylori-IgG between the two groups ( P=0.281). There were significant differences between RE group and control group in gender composition, age, body mass index (BMI), smoking, alcohol consumption, education level and mucosal active inflammation. Multivariate regression analysis showed that RE was negatively correlated with gastric mucosa active inflammation [ OR=0.754 (95% CI 0.600-0.949), P=0.016], and positively correlated with male [ OR=4.231 (95% CI 3.263-5.486), P<0.001], age ≥60 years, BMI≥24 kg/m 2 [ OR=1.540 (95% CI 1.220-1.945), P<0.001]. Compared to those aged 40-49 years and 50-59 years, the odds ratio ( OR) of RE in these aged ≥60 years were 1.566 (95% CI 1.144-2.143, P=0.005) and 1.405 (95% CI 1.093-1.805, P=0.008). Conclusion:RE is more closely related to H. pylori present infection. Multivariate analysis showed that RE is negatively correlated with active inflammation of gastric mucosa caused by H. pylori infection, and positively correlated with male, overweight and aged ≥60 years.

Multidrug resistance (MDR) is one of the main causes leading to the failure in cancer treatment. Differential proteins between esophageal squamous cell carcinoma (ESCC) cell line EC9706 and its cisdiamminedichloroplatinum (CDDP)-resistant subline EC9706/CDDP revealed by quantitative analysis may provide deeper insights into the molecular mechanisms of MDR implicated in ESCC. EC9706/CDDP was generated by exposure of its parental sensitive EC9706 to a step-wise increase of CDDP concentration during EC9706 cultivation. The stable isotope labeling with amino acids in cell culture (SILAC) was used to label EC9706 and EC9706/CDDP with heavy and light medium, separately. Mixed peptides derived from EC9706 and EC9706/CDDP were analyzed by high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS/MS) and subsequently subjected to bioinformatics analysis to identify differential proteins between EC9706 and EC9706/CDDP. Compared to parental EC9706, EC9706/CDDP manifested phenotypes of slow proliferation, cell pleomorphology, atypia and increased resistant-index 3.23. Seventy-four differential proteins identified in the present study belongs to various families with multiple functions, such as cytoskeleton (20%), energy metabolism (11%), transcription regulation and DNA repair (11%), redox homeostasis (9.5%), protein biosynthesis and mRNA processing (12%), ribosome constituent (8.1%), molecular chaperone (8.1%), immunity/inflammation (5.4%), intracellular transport (5.4%) and nucleosome assembly (2.7%), which indicated that development of MDR is a complicated process involving dysregulation of multiple molecules and pathways. The data is of great value for in-depth elucidation of molecular mechanisms of the MDR implicated in ESCC and may represent potential molecular targets for future therapeutic development.
Carcinoma, Squamous Cell ; metabolism ; pathology ; Cell Line, Tumor ; Chromatography, High Pressure Liquid ; Cisplatin ; pharmacology ; Drug Resistance, Multiple ; Drug Resistance, Neoplasm ; Esophageal Neoplasms ; metabolism ; pathology ; HSP70 Heat-Shock Proteins ; metabolism ; Humans ; Intramolecular Oxidoreductases ; metabolism ; Isotope Labeling ; Macrophage Migration-Inhibitory Factors ; metabolism ; Proteome ; metabolism ; Proteomics ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry ; Thioredoxins ; metabolism

Gastric cancer is a malignant tumor characterized by high morbidity and mortality. With the development of molecular biol-ogy technology and the emergence of various new omics detection techniques in recent years, molecular epidemiologists of gastric cancer have conducted extensive studies on the genetic and host factors, as well as gene-environment interactions associated with ex-posure to environmental factors in gastric cancer. In addition, epidemiologists have studied the evolution of precancerous gastric le-sions, the development of gastric cancer, and explored relevant biomarkers to provide major evidence for the prevention and control of gastric cancer. This review summarizes the latest advances in the molecular epidemiology of gastric cancer, including existing evi-dence in studies for candidate-approach-based serum/plasma biomarkers, genome-wide association, whole-exome sequencing, tissue microarrays, as well as studies on metabolomics and microbiomes. We expect to provide insights into the future of molecular epidemi-ology studies in gastric cancer, promoting etiologic research, and the precise prevention and control of gastric cancer.