OBJECTIVE: To retrospectively analyze the long-term curative effects and prognostic factors of 137 cases of NPC patients treated with intensity modulated radiation therapy. METHOD: The three-dimensional conformal intensity modulated radiation therapy adopted for the design plan of 137 cases of newly diagnosed and no distant metastatic NPC patients. Evaluation of short-term clinical efficacy was made according to the efficacy evaluation criteria of WHO solid tumor one month after radiotherapy. During the 2 years after radiotherapy, re-examinations were made every 3 months. Two years later, re-examinations were made every 6 to 12 months, and the routine examinations included clinical body check, nasopharyngoscope, CT, B Chao, MRI, chest X-ray, bone scan, so as to understand the condition of cavum nasopharyngeum, cervical lymph nodes and cranial nerves. Life table method was adopted to calculate the overall survival rate, Kaplan-Meier method was used to calculate relapse-free survival rate, distant metasis-free survival rate and disease-free survival rate. Log-rank test was used for univariate analysis of 14 clinical factors which may exert influences on prognosis. Multivariate analysis was performed with the Cox regression model, P < 0.05 is of statistical significance. RESULT: (1) Of the 137 cases, the overall survival rates of 1-year, 3-year and 5-year were 98.5%, 90.3%, 74.6% respectively, relapse-free survival rates were 97.0%, 81.9%, 66.7% respectively; distant metasis-free survival rates were 96.3%, 80.5%, 56.0% respectively; disease free survival rates were 95.6%, 76.9%, 43.8% respectively. (2) Univariate analysis revealed that the influences of T-staging,N-staging, 92 Fuzhou clinical staging, combined chemotherapy, skull base bone destruction, injury of cranial nerve, retropharyngeal lymph node metastasis, residual disease, total time of IMRT on prognosis were of no statistical significance(with all the P values lower than 0.05) The influences of gender, nationality, age, pathological type and anemia or on prognosis were of no statistical significance(with all the P values higher than 0.05). (3) T-staging, skull base bone destruction, injury of cranial nerve, retropharyngeal lymph node metastasis, N-staging, combined chemotherapy, residual disease, total time of IMRT, and anemia were drawn into Cox model, and the results showed that N-staging, combined chemotherapy, injury of cranial nerve, residual disease, and total time of IMRT were independent factors that affect prognosis (with all P values lower than 0.05). CONCLUSION IMRT obviously has advantages than conventional radiotheraphy in treating NPC patients. N-staging, injury of cranial nerve, combined chemotherapy, residual disease, and total time of IMRT are the main factors that affect prognosis.
Adult ; Aged ; Carcinoma ; Disease-Free Survival ; Female ; Follow-Up Studies ; Humans ; Lymph Nodes ; Lymphatic Metastasis ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms ; mortality ; radiotherapy ; Neoplasm Recurrence, Local ; Prognosis ; Radiotherapy, Intensity-Modulated ; Retrospective Studies ; Skull Base ; pathology ; Survival Rate

Objective To study the optimum inclusion process for volatile oil from Tongketing capsules. Methods The study was carried out with orthogonal test at different levels. The utilization ratio of volatile oil was used as the index to optimize the inclusion process. Results The optimum preparation conditions for inclusion were: oil: ?-cyclodextrin: water was 1: 4: 40 (V: V: V). The inclusion time was 10rain and inclusion complexes was dried at 70℃. Conclusion This optimized process is simple, efficient and can be used for the production of Tongketing Capsule on a large scale.

[Objective]To study the expression of Cathepsin B,Caspase-3 and to explore the significance of their expression initially after acute spinal cord injury in rats.[Method]The spinal cord injury of the healthy adult SD rats (78) was induced with Nystrom’s way by the moderate compression at the level of T8 and T9 spinal cord. HE methods were used to detect the pathologic change of spinal cord. The expression of Cathepsin B and Caspase-3 were detected by immunohistochemical study. Besides,using the terminal deoxynucleotidyl transferase mediated DUTP nick and labeling (TUNEL) methods to detect the level of the apoptosis of neural cells.[Result]There were few expressions of Cathepsin B, Caspase-3 and TUNEL positive cells in normal and sham operated spinal cord as detected by immunohistochemical study. The number of positive cells of Cathepsin B increased clearly at 3 days,reached a peak at 5 days,and was constant at 7 days after injury. While the expression of Caspase-3 increased obviously at 8 hours,got to a peak at 3 days,and was lowest at 7 days. And the number of positive cells of TUNEL also increased obviously at 8 hours,got to a peak at 3 days,and was lowest at 7 days. There were significance differences of morphological and position of positive cells between Cathepsin B and Caspase-3 or Tunel. [Conclusion]Caspase-3 protein expressions are enhanced in combination with neuronal apoptosis,while Cathepsin B may involve in the secondary injury by inflammatory cells after acute spinal cord injury.

Objective To evaluate the application of chromogranin A (CgA) as a marker in the diagnosis of prostate cancer. Methods Serum chromogranin A were detected by means of ELISA technique in 35 cases of prostate carcinoma,10 cases of benign prostate hyperplasia and 30 cases of healthy subjects. Results Serum CgA [(162?12.5)ng/ml] of patients with prostatic carcinoma was significantly higher than those of healthy subjects and of BPH( P

Objective To study the function difference of T helper cells(Th)and dendritic cells (DCs) in patients with different hepatitis B virus (HBV) genotypes. Methods IL-12,IL-2 and IL-4 released by Th was measured in 30 patients with different HBV genotypes. After DCs were differentiated and proliferated, DCs’ configuration and amounts were observed, and IL-12 released by DCs was measured in 20 patients. Then the data were statistical analyzed. Results IL-12, IL-2 and IL-4 were significantly higher in patients with hepatitis B than the controls (t=2.9272,t=2.7350,P

[Objective]To explore the effect of combined therapy of Chinese and western medicine on Bell paralysis.[Methods] Randomly divide 62 cases of Bell paralysis into 2 groups who were treated respectively with combined therapy of Chinese and western medicine and western medicine.[Results] For effective rate,the treatment group(91.1%) was markedly higher than control group(67.8%),there was obvious difference between the 2 groups.[Conclusions] Combined therapy of Chinese and western medicine has marked therapeutic effect on Bell paralysis.

[Objective]To evaluate the clinical and radiological findings in 56 patients with multilevel lumbar spinal stenosis in pre-and post-surgical treatment. [Method]From 1998 and 2002,56 patients suffering from multilevel lumbar spinal stenosis were included in a retrospective clinical study.Plain radiographs,MRI and/or CT myelograms were obtained preoperatively.The patients were assessed clinically with Oswestry disability index(ODI),visual analog scale(VAS) and the Beaujon functional score.Surgical procedure included wide posterior decompression and fusion using a transpedicular instrumentation system and bone grafts. [Result]After a mean follow-up of 2.2(2~2.6) years,the patients' clinical improvement by ODI,VAS was statistically significant and the Beaujon score was significantly improved(P

AIM: To observe the clinical effecacy of Aidi Injection (Radix Ginseng, Radix Astragali, Mylabris, Radix et Caulis Acanthopanacis Senticosi) combined with chemotherapy in the treatment of malignant advanced tumor. METHODS: Patientes (106 cases) with advanced malignant tumor were randomized into two groups: therapeutic group (56 cases) and control group (50 cases). The therapeutic group:the chemotherapy combined with Aidi Injection (60～80mL), once a day, for 10 days. The control group:the chemotherapy alone. Two groups mainly used PTX、DDP、EPI、5 FU、VP16 etc for chemotherapy. RESULTS: The therapeutic group has different degrees of improvement in life quality, short term efficacy, leukooytai drop and T cellular subfamily, etc. There was markedly difference between the therapeutic group and the control group ( P

Objective To study the clinical efficacy of electromyography biofeedback on dystonia after hepatolenticular degeneration.Methods 40 patients with dystonia after hepatolenticular degeneration were divided into treatment group (n=20) and control group (n=20).All patients were treated with copper-cleaning, clonazepam and benzhexol hydrochloride etc., and acupuncture. The treatment group wastreated with electromyographic biofeedback in addition. They were assessed with modified Ashworth scale, modified Barthel index, and ankledorsiflexion active range of motion. Results The lower extremities function of all patients improved after treatment (P<0.05) and thetreatment group improved more than the control group (P<0.05). Conclusion Electromyography biofeedback is more effective to improvethe lower extremities function and activity of daily living in patients with hepatolenticular degeneration following dystonia.

Flammulin, an anti-tumor protein, was purified from the aqueous extract of basidiomes of Flammulina Velutipes. Purified flammulin emulsified with Freund's adjuvant was injected subcutaneously into New Zealand white rabbits. After several immune enhancements, these animals were bled and sera were separated. Antiserum against flammulin in Western blots were applied to determine if flammulin be present in the liquid state culture or fruiting body. The result showed that anti-flammulin serum could recognize the aqueous extract of fruiting body in SDS-PAGE gels under the reducing conditions, no flammulin was detected in mycelia of Flammulina Velutipes.