Objective] Summarize clinical experience about treatment of scapulohumeral periarthritis with neck rotation and shoulder movement proposed by the well-known doctor Wang Youren from Beijing Massage Hospital. [Method] Observe the clinical treatment of scapulohumeral periarthrit, organize the related medical records and draw on the treatment thoughts and operational method.[Result] Mr. Wang thinks that there is close connection between the scapulohumeral periarthriti and neck in the perspective of pathogenesis and anatomy and physiology. It will have better treatment effect if giving the fine-tuning to the neck while treating the scapulohumeral periarthritis. [Conclusion] Mr. Wang Youren has unique clinical treatment experience in the area of scapulohumeral periarthritis, and this treatment has significant effect. Thus it is worth of spreading.

OBJECTIVE:To discuss the choice and the route for the realization of brand strategy feasible to Chinese pharmaceutical industry(CPI)in the current and future competition environment.METHODS:Reasons for the lack of influential brands were analyzed based on the current practical situation of CPI,then some countermeasures were put forward.RESULTS&CONCLUSIONS:Knowing the importance of brands and strengthening brands construction are the key issues for CPI to consider in the market competition,however,establishing national or even international brands is the long-term target of the future development of CPI.

Objective: To compare the expression levels of FOXP3 mRNA in the peripheral blood mononuclear cells(PBMC) of hepatitis B patients and healthy persons.Methods: Real-time fluorescence relative quantitative RT-PCR was used to determine the FOXP3 mRNA levels in the PBMCs from 25 patients with chronic hepatitis B and 11 healthy subjects.Results:The FOXP3 mRNA levels were significantly higher in the hepatitis B patients than in the normal subjects(P0.05).Conclusion: The high expression of FOXP3 might be an important factor for the persistence of HBV infection.

Thyroid papillary carcinoma harbors higher rate of lymph node metastasis, so neck dissection is an important part of the treatment. Due to better prognosis, attention to postoperative function and appearance of the patients increased. Low-collar incision with cervical sensory nerve plexus preservation modiifed neck dissection can improve appearance and function in postoperative patients and is gradually applied in clinic. This review mainly examines the thoroughness and safety of this new neck dissection in thyroid papillary carcinoma patients.

Objective To develop a quick, simple and sensitive method for determination of sulfuric acid mist in the air by ion chromatography. Methods The samples were collected by Nillipore filter, Na2CO3-NaHCO3 solution was used and the samples were analyzed by using ion-chromatography as well as external standard curve. Results The linear range of the method was 0.5-10.0 ?g/ml and the correlation coefficient was 0.999 8. The RSD for the test accuracy and variation of repeat tests within one day or between the different days was

Adult ; Facial Nerve Injuries ; etiology ; Foreign Bodies ; complications ; Humans ; Male ; Neck ; Parotid Gland

In order to promote the concept of animal welfare teaching in medical university,this paper introduces animal welfare,3R education and our current status of animal experiments ; taking the practice of animal welfare teaching in southern medical university,focusing on the teaching content,teaching methods,teaching resources development and other aspects of the animal welfare teaching reform.

BACKGROUND: The selection of carrier plays an essential role in the research of applying tissue-engineering to fix the peripheral nerves. An ideal carrier would be one that is similar to extracellular matrix and that it has biocompatibility with in vivo cells.OBJECTIVE: To evaluate the biocompatibility of hippocampal neural stem cells with collagen and gelatin sponge in vitro and to probe into the feasibility of using the materials as biomaterial scaffold in peripheral nerve tissue engineering.DESIGN: A randomized and controlled trial.SETTING: Department of Otorhinolaryngology of the First Affiliated Hospital of Zhengzhou University; Department of Anatomy of the School of Basic Medical Sciences of Zhengzhou University.MATERIALS: The experiment was carried out from July to December 2005 at the Laboratory of Neurobiology of the Department of Anatomy of the School College of Basic Medical Sciences of Zhengzhou University.Twelve New born (＜ 24 hours) clean grade guinea pigs of either gender with a body mass of 50-70 g, were provided by the Experimental Animals Center of Zhengzhou University School of Medicine.METHODS: The new born (＜ 24 hours) guinea pigs were anesthetized intraperitoneally with 10 g/L chloral hydrate and sterilized in 0.75 volume fraction of alcohol. Hippocampal tissue was resected from the brain under a surgical microscope. Hippocampus neural stem cells were cultured in vitro. The cultured cells of two generations were suspended at a density of 1 ×1010L-1 and respectively combined with collagen and gelatin sponge.The number of cells was counted and histological changes were observed under an inverted phase microscope and scanning electron microscope after 7 days, and the adhesion rate of the two materials to the cells were measured.MAIN OUTCOME MEASURES: ①Growth of the neural stem cells and their adhesion to the collagen and gelatin sponge were observed and the total number of the cells and adhesion rate with carrier were measured. ②The morphological changes of differentiation cells by immunocytochemical staining.RESULTS: ①Hippocampus neural stem cells could grew on the collagen and gelatin sponge and attached to them gradually. The adsorption rate of collagen was higher than thai of gelatin sponge (37.17 % and 14.87 %,x2=4.819,P ＜ 0.05). ② There was no significant difference in the total number of the cells in the control group, collagen group and gelatin sponge group [(53.17±3.5)×104,(53.25±2.6)×104, (52.04±4.05)×104,F=0.233,P ＞ 0.05].CONCLUSION: Two biomaterials (collagen and glutin sponge), especially collagen, have good biocompatibility with hippocampus neural stem cells from the guinea pigs and can be used safely as scaffold materials in peripheral nerve tissue engineering.

Background Retinopathy of prematurity (ROP) causes blindness due to retinal vasculopathy caused by abnormal oxygen dynamics.Studies have clarified the pivotal role of vascular endothelial growth factor (VEGF) in the development of ROP,while the studies on the role of VEGF receptor (VEGFR) in ROP are fewer.Objective This study was to evaluate the relationship between the expressions of VEGFR-1 and VEGFR-2 in retina and post-birth time in the mice with oxygen-induced retinopathy (OIR).Methods Sixty 7-day-old (P7) SPF C57BL/6J mice together with lactating female mice were fed in the environment with (75±2)％ oxygen concentration for 5 days and then returned to normal air to establish OIR models,and other 60 matched mice were kept in normal air environment as the control group.At P8,P11,P12,P13,P14,and P17,both eyes of each mouse were enucleated to prepare the retinal sections.Retinal blood vessels were examined by hematoxylin and eosin stain under the light microscope.Real-time fluorescence quantitative PCR and immunohistochemistry were used to detect the expressions of VEGFR1 mRNA and VEGFR2 mRNA and their proteins in mouse retinas,respectively.The use and care of the animals complied with the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission.Results Many vascular endothelial cell nucleus broke through the internal limiting membrane were seen with disorganized cell proliferation under the internal limiting membrane and retinal neovascularization bud in the P13,P14 and P18 mice in the OIR group,but no similar manifestation was found in the mice of the normal control group.The relative expression values of VEGFR-1 mRNA in retinas were significantly higher only in P12,P13,P14 and P18 mice in the OIR group than those in the normal control group (P=0.046,0.000,0.000,0.042),but the expression values of VEGFR-2 mRNA were all increased in the retinas of P8,P11,P12,P13,P14 and P18 mice in the OIR group,showing significant differences in comparison with the normal control group (all at P=0.000).No considerable difference was found in the expression level of VEGFR-1 protein in P8,P11,P12,P13,P14 and P18 mice between the two groups (M=50.00,36.00,41.00,31.00,28.00,36.00,all at P＞ 0.05).The expression levels of VEGFR-2 protein in retinas of P8 and P11 were close between the two groups (all at P＞0.05).Gradually attenuated expressions were seen in VEGFR-2 protein in P12,P13 mice of the normal control group,however,the expressions were enhanced in the OIR group,with significant differences between the two groups (all at P＜0.01).Enhanced expressions of VEGFR-2 protein were found in P14 mice in both groups,but stronger expressions were in the OIR group (P＜0.01).The positive response of the expression of VEGFR-2 protein was weaker in P18 mice in the normal control group but peaked in the OIR group,showing significant difference between them (M=20.11,P＜0.01).Conclusions VEGFR participates in the neovascularization of OIR mouse,and the effect of VEGFR-2 is stronger than that of VEGFR-1 in the development of OIR neovascularization.

Background Neovascular diseases such as retinopathy of prematurity often leads to irreversible vision loss.The study on oxidative damage mechanism is becoming more and more important.Whether brain derived neurotrophic factor (BNDF) has protection to retinal ganglion cells(RGCs) has few research reports.Objective The study was to investigate the expression changes of BDNFin mouse retinas of oxygen-induced retinopathy (OIR).Methods Thirty SPF C57BL/6J immature rats were divided into OIR group and normal control group and to fifteen rats for each group.OIR models were established by raising 7-day-old (P7) mice together with maternal mouse in (75±3) ％ oxygen environment for 5 days and then returned to the normal air environment,and the mice of the normal control group were raised in the normal air environment.The P17 mice were sacrificed for retinal histopathological examination by hematoxylin and eosin staining, and the number of vascular endothelial cell nucleus extending the inner limiting membrane was counted.Whole retinal mounts were prepared after fluorescein isothiacyanate-dextran (FITC-dextran) (9 ml/kg) was retrosorbitally injected,and the distribution of retinal vessels was observed in P17 mice.The relative expression levels of BNDF in retinas were detected in P13, P15, P17 mice, and the results were compared between the normal control group and the OIR group.Results Histopathological examination showed that retinal inner limiting membrane was smooth in the normal control group, but a lots of vascular endothelial cell nucleus extending the inner limiting membrane were seen under the optical microscope in the OIR group.The number of the vascular endothelial cell nucleus extending the inner limiting membrane was 1.70±0.68 in the normal group and 45.3±3.13 in the OIR group, showing significant difference between them (t =86.5, P =0.00).Whole retinal mount revealed that normal retinal vessels and network-like capillaries were exhibited in the mice in the normal group, while tortuous vessels,capillary loss and non-perfusion areas were revealed in the OIR group on the whole retina mounts.The relative expressing levels of BDNF in retinas were 263.992±9.451 and 218.432±9.710 in P15 and P17 mice in the OIR group,which were significantly higher than 230.324±7.779 and 115.846±7.305 in the normal control group (t=14.2,42.3 ,P＜0.05).Conclusions OIR can be inhibited by increasing the expression of BNDF.