Objective To investigate the cause of shallow anterior chamber after filtered operation and determine how to treat shallow anterior chamber.Methods 196 eyes of 168 cases who had suffered from glaucoma underwent trabeculectomy.The number of eyes and the extent of shallow anterior chamber were observed and treated correspondently.Results There were 39 eyes with shallow anterior chamber among 196 eyes,and the percentage was 20%.Over fluent drainage,aqueous humor leaking,choroidal detachment and malignant glaucoma were the main causes of shallow anterior chamber after trabculectomy.Careful operation good,good reconstruction and timely management were crucial to decrease the complications.Conclusion Shallow anterior chamber can not be fully preventable,but its happening can be minimized and cured effectively.It is significant to protect visual function.

Objective To investigate the characteristics and influencing factors leading to sudden cardiac death (SCD) of incarcerated prisoners. Methods Seventy-five SCD cases of prisoners between 2000 and 2013 in Henan province were collected, and environment, psychological and physical factors were retro-spectively analyzed. Combined with histopathological results, specific factors of SCD were also studied. Results In the 75 cases, 21 cases (28%) had definite chronic past medical histories, and 75 cases (100%) had cardiovascular disease confirmed by autopsy. Conclusion Due to presence of the potential cardiac diseases, special incarcerated environment, psychological stress, and body-restraint might be the precipitat-ing factors in SCD of those prisoners.

BACKGROUND: The selection of carrier plays an essential role in the research of applying tissue-engineering to fix the peripheral nerves. An ideal carrier would be one that is similar to extracellular matrix and that it has biocompatibility with in vivo cells.OBJECTIVE: To evaluate the biocompatibility of hippocampal neural stem cells with collagen and gelatin sponge in vitro and to probe into the feasibility of using the materials as biomaterial scaffold in peripheral nerve tissue engineering.DESIGN: A randomized and controlled trial.SETTING: Department of Otorhinolaryngology of the First Affiliated Hospital of Zhengzhou University; Department of Anatomy of the School of Basic Medical Sciences of Zhengzhou University.MATERIALS: The experiment was carried out from July to December 2005 at the Laboratory of Neurobiology of the Department of Anatomy of the School College of Basic Medical Sciences of Zhengzhou University.Twelve New born (＜ 24 hours) clean grade guinea pigs of either gender with a body mass of 50-70 g, were provided by the Experimental Animals Center of Zhengzhou University School of Medicine.METHODS: The new born (＜ 24 hours) guinea pigs were anesthetized intraperitoneally with 10 g/L chloral hydrate and sterilized in 0.75 volume fraction of alcohol. Hippocampal tissue was resected from the brain under a surgical microscope. Hippocampus neural stem cells were cultured in vitro. The cultured cells of two generations were suspended at a density of 1 ×1010L-1 and respectively combined with collagen and gelatin sponge.The number of cells was counted and histological changes were observed under an inverted phase microscope and scanning electron microscope after 7 days, and the adhesion rate of the two materials to the cells were measured.MAIN OUTCOME MEASURES: ①Growth of the neural stem cells and their adhesion to the collagen and gelatin sponge were observed and the total number of the cells and adhesion rate with carrier were measured. ②The morphological changes of differentiation cells by immunocytochemical staining.RESULTS: ①Hippocampus neural stem cells could grew on the collagen and gelatin sponge and attached to them gradually. The adsorption rate of collagen was higher than thai of gelatin sponge (37.17 % and 14.87 %,x2=4.819,P ＜ 0.05). ② There was no significant difference in the total number of the cells in the control group, collagen group and gelatin sponge group [(53.17±3.5)×104,(53.25±2.6)×104, (52.04±4.05)×104,F=0.233,P ＞ 0.05].CONCLUSION: Two biomaterials (collagen and glutin sponge), especially collagen, have good biocompatibility with hippocampus neural stem cells from the guinea pigs and can be used safely as scaffold materials in peripheral nerve tissue engineering.

Objective:To evaluate the effect of concentrate growth factor extract(CGFe)on the proliferation and differentiation of MC3T3-E1 osteoblasts.Methods:MC3T3-1 cells were cultured with and without CGFe respectively.At 1 ,3,5 d of culture the pro-liferation of the cells was detected by MTT assay,the activity of alkaline phosphatase (ALP)of the cells was examined by ALP kit;at 1 4,21 d of culture the calcium nodus formation was observed by Alizarin red staining;RUNX2 and OSX expression was quantified by real-time PCR.Results:With the extension of culture time,the proliferation,ALP activity,the calcium nodus and the level of RUNX2 and OSX mRNA of the experimental group were increased more than those of the control group.Conclusion:CGFe may pro-mote the proliferation and differentiation of osteoblasts.

AIM: To investigate the changes of first order kernel ( FOK ) and second order kernel ( SOK ) of multifocal electroretinogram( mf-ERG) in detecting the early retinal abnormalities in sub-clinical stage of diabetic retinopathy. ·METHODS: Totally 32 patients ( 58 eyes ) with type 2 diabetes mellitus ( DM ) without apparent diabetic retinopathy( DR) were detected by mf-ERG, from June 2014 to May 2015. Thirty cases ( 60 eyes ) of normal control group had also been taken to compare the difference of the amplitude and latency between the two groups. ·RESULTS: Compared with the control group: there was no statistical difference in the FOK b-wave latency of the diabetic group, and the FOK b-wave amplitude was significantly decreased (t=3. 099, P=0. 012). The SOK b-wave latency in diabetes group was statistically delayed (t=2. 643, P=0. 025), and the SOK b-wave amplitude statistically decreased (t=4. 833, P<0. 01). There was no statistically difference in the a-wave latency and amplitude of FOK and SOK. The b-wave amplitude of FOK and SOK was negatively correlated with the course of the disease. However, the b-wave amplitude was not statistically correlated with the duration of diabetes mellitus. ·CONCLUSION: The function of the post-polar outer and inner retina detected by FOK and SOK had been damage even before retinopathy. The anomaly is mainly reflected by the decreasing amplitude of b-wave.

0. 05). Level of sCD40L was significantly decreased in the tirofiban group after PCI compared with the control (3.17?1.01 ?g/L vs 4.05?0.96 ?g/L, P

Objective To investigate the difference on postoperative hepatic functions between laparoscopic and conventional surgery for colorectal cancers. Methods In this prospective study, a laparoscopic group ( n =20) was compared with an open group ( n =20). Blood samples were obtained at 1, 24, 48, and 72 hours postoperation respectively to perform liver function tests, including total bilirubin (TBil), albumin (Alb), gamma-glutamyl transpeptidase (?-GT), alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Results Of the both groups, serum Alb and ALP levels decreased, and TBil and GGT levels remained unchanged. Postoperative ALT and AST levels transiently increased by threefold and returned to near baseline levels at 72 hours. There was no postoperative liver failure or mortality in both groups. Conclusions Laparoscopic colorectal surgery results in postoperative elevation of hepatic transaminases but does not adversely alter hepatic functions to any greater extent than open colorectal surgery.

Objective To evaluate the changes of some humoral immune indices of patients with hypersplenism treated by radiofrequency ablation (RFA). Methods Totally 33 patients met inclusion criteria were randomly assigned to receive RFA (17 patients) as trial group and splenectomy (16 patients) as control group. The level of serum immune globulin,complement and tumor necrosis factor (TNF) in venous blood was determined preoperatively and postoperative 1 week,1 month,and 6 months. We evaluated intergroup differences in immune function by comparing the indices. Results In the trial group,no significant differences were found in humoral immune indices between postoperative and preoperative values. Although the level of postoperative serum complement did not change significantly,serum IgM was lowered significantly (P0.05). Conclusion Because part of the spleen is remained in RFA,the humoral immune function is reserved better than that of splenectomy.

Objective To examine the serum Tuftsin content of hypersplenism patients treated by radiofrequency ablation(RFA) or splenectomy, and compare the influence of two curative methods.Methods Totally 33 patients of hypersplenism were randomly assigned to receive RFA(n=17) and splenectomy(n=16).Before and after operation,the serum Tuftsin content was detected by reverse phase-high performance liquid chromatography.Results Relief of hypersplenism was achieved in both group.Because the spleen was resected,serum Tuftsin content in splenectomy group significantly decreased(P0.05).Conclusion Radiofrequency ablation for hypersplenism has good curative effect and significantly preserves body's resistance to infections and tumors.

Objective: To evaluate the application value of large flat-panel multifunction digital machine in digestive system. Methods: Exam the gastrointestinal tract and biliary with dynamic detector, and then observe its advantages and disadvantages. Results: All the 13 gastrointestinal cancer lesions and 15 inflammatory lesions were shown clearly, including mucosa. Biliary tomography found two small stones, which were undetected by the general X-ray photography. The excellent rate of the photo was improved to 98%. Conclusion: The advantages of the flat panel detector include the wide range of display, clear image of digital pulse fluoroscopy spot film, the improvement of the diagnostic accuracy and the reduction in radiation dose. The disadvantage is that it is more inconvenient than the remote cradle gastrointestinal machine in rotating the position of the patient.