“Exercise Guideline 2006” was published by Ministry of Health, Labour and Welfare of Japan to maintain and promote the health of people and to prevent lifestyle-related diseases through improving their habits of physical activity and exercise. In “Exercise Guideline 2006”, define ‘Ex’ as a unit of quantity of physical activity, and recommend to increase the ‘Ex’ value for physical activity to maintain the health of people. We investigated the effect of physical activity instruction using this new guideline on the profiles of dyslipidemia outpatients.We gave monthly physical activity instructions to 25 outpatients with dyslipidemia according to “Exercise Guideline 2006” and evaluated body composition and blood profiles 6 months after the instruction was started.Weekly physical activities were significantly increased by the instruction, from 7.1±4.1 Ex (pre) to 15.5±10.6 Ex (post). As the increased amount of physical activity in each subject was varied with individuals, we classified the subjects into two groups (increased group, or non-increased group) according to the increased amount of activity. In the subjects in increased group, significant decrease in body weight and serum triglyceride, and significant increase in serum HDL cholesterol were observed at 6-month point. There is no correlation between the variations in body compositions and the variations in triglyceride or HDL cholesterol levels.In conclusion, we suggest that physical activity instruction using “Exercise Guideline 2006” might be a useful and convenient strategy to improve lipid profiles in dyslipidemia patients.